(Circular) (Six-base) MAPSORT of: pFN21AF0417.seq Check: 2074 from: 1 to: 6322 pFN21AF0417 6322 bp With 182 enzymes: SgfI * October 20, 2009 10:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2073 3520 2073 Size: 1447 4875 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4132 278 Size: 53 83 186 936 2596 2468 Fragments arranged by size: 2596 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3665 1937 Size: 1728 4594 Acc65I G'GTAC_C Cuts at: 3643 3643 Size: 6322 AclI AA'CG_TT Cuts at: 1563 4449 4822 1563 Size: 2886 373 3063 Fragments arranged by size: 3063 2886 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2536 4385 5769 6037 1632 Size: 904 1849 1384 268 1917 Fragments arranged by size: 1917 1849 1384 904 268 AfeI AGC'GCT Cuts at: 2324 2324 Size: 6322 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6125 AflIII A'CryG_T Cuts at: 1203 2578 2580 5221 6198 1203 Size: 1375 2 2641 977 1327 Fragments arranged by size: 2641 1375 1327 977 2 AgeI A'CCGG_T Cuts at: 5153 5153 Size: 6322 AhdI GACnn_n'nnGTC Cuts at: 5051 5051 Size: 6322 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6322 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2579 1184 Size: 1395 4927 AlwNI CAG_nnn'CTG Cuts at: 2662 4065 5637 2662 Size: 1403 1572 3347 Fragments arranged by size: 3347 1572 1403 ApaI G_GGCC'C Cuts at: 2892 2928 3163 3184 3373 2892 Size: 36 235 21 189 5841 Fragments arranged by size: 5841 235 189 36 21 ApaLI G'TGCA_C Cuts at: 2462 4379 5535 2462 Size: 1917 1156 3249 Fragments arranged by size: 3249 1917 1156 ApoI r'AATT_y Cuts at: 1493 3631 3857 4009 6118 1493 Size: 2138 226 152 2109 1697 Fragments arranged by size: 2138 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4876 160 Size: 872 3844 1606 Fragments arranged by size: 3844 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6322 AvaI C'yCGr_G Cuts at: 1870 1943 2885 2943 3017 3115 3156 3162 3214 Size: 73 942 58 74 98 41 6 52 Cuts at: 3214 3313 3647 1870 Size: 99 334 4545 Fragments arranged by size: 4545 942 334 99 98 74 73 58 52 41 6 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2567 2600 2567 Size: 33 6289 BamHI G'GATC_C Cuts at: 2071 2760 3062 3652 4019 2071 Size: 689 302 590 367 4374 Fragments arranged by size: 4374 689 590 367 302 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2135 2878 2953 3350 3643 Size: 325 217 627 348 743 75 397 293 Cuts at: 3643 5098 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 743 627 397 348 325 293 217 75 BanII G_rGCy'C Cuts at: 1391 2376 2892 2928 2981 3163 3184 3373 3641 Size: 985 516 36 53 182 21 189 268 Cuts at: 3641 1391 Size: 4072 Fragments arranged by size: 4072 985 516 268 189 182 53 36 21 BbeI G_GCGC'C Cuts at: 2957 2957 Size: 6322 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2308 928 Size: 971 409 4942 Fragments arranged by size: 4942 971 409 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4513 4547 1903 Size: 34 2576 34 3678 Fragments arranged by size: 3678 2576 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3579 4214 5424 3579 Size: 635 1210 4477 Fragments arranged by size: 4477 1210 635 BclI T'GATC_A Cuts at: 1548 1548 Size: 6322 BfrBI ATG'CAT Cuts at: 3045 3045 Size: 6322 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2673 2814 Size: 107 122 71 631 605 337 664 141 Cuts at: 2814 2887 4933 136 Size: 73 2046 1525 Fragments arranged by size: 2046 1525 664 631 605 337 141 122 107 73 71 BglII A'GATC_T Cuts at: 2265 6317 2265 Size: 4052 2270 BlpI GC'TnA_GC Cuts at: 3739 3739 Size: 6322 Bme1580I G_kGCm'C Cuts at: 1790 2466 2883 2892 2928 3163 3184 3373 4383 Size: 676 417 9 36 235 21 189 1010 Cuts at: 4383 5539 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 1010 676 417 235 189 36 21 9 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6322 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3185 3614 4053 Size: 416 53 83 399 24 1768 429 439 Cuts at: 4053 5011 442 Size: 958 1753 Fragments arranged by size: 1768 1753 958 439 429 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6322 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2236 2236 Size: 6322 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2254 3274 3280 3431 4982 1239 Size: 1015 1020 6 151 1551 2579 Fragments arranged by size: 2579 1551 1020 1015 151 6 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2116 4447 5312 5610 2116 Size: 2331 865 298 2828 Fragments arranged by size: 2828 2331 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2438 4985 882 Size: 617 939 2547 2219 Fragments arranged by size: 2547 2219 939 617 BsaAI yAC'GTr Cuts at: 493 1204 2581 4113 493 Size: 711 1377 1532 2702 Fragments arranged by size: 2702 1532 1377 711 BsaBI GATnn'nnATC Cuts at: 2270 6316 2270 Size: 4046 2276 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2954 4129 4511 5146 Size: 53 83 186 936 1421 1175 382 635 Cuts at: 5146 275 Size: 1451 Fragments arranged by size: 1451 1421 1175 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2074 2902 4755 5153 5427 5574 5985 2074 Size: 828 1853 398 274 147 411 2411 Fragments arranged by size: 2411 1853 828 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2069 2099 2837 2867 3247 Size: 30 165 30 666 30 738 30 380 Cuts at: 3247 3277 1178 Size: 30 4223 Fragments arranged by size: 4223 738 666 380 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2456 3428 1186 Size: 1270 972 4080 Fragments arranged by size: 4080 1270 972 BseYI C'CCAG_C Cuts at: 2041 2723 2911 3026 3110 3497 5525 6021 2041 Size: 682 188 115 84 387 2028 496 2342 Fragments arranged by size: 2342 2028 682 496 387 188 115 84 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5749 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3772 4533 5561 1540 Size: 275 186 1771 761 1028 2301 Fragments arranged by size: 2301 1771 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2217 2466 3552 3558 4383 4468 5539 2217 Size: 249 1086 6 825 85 1071 3000 Fragments arranged by size: 3000 1086 1071 825 249 85 6 BsmI GAATG_Cn' Cuts at: 3833 3926 3833 Size: 93 6229 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2217 2376 2466 2883 2892 2928 2981 Size: 399 427 159 90 417 9 36 53 Cuts at: 2981 3163 3184 3373 3552 3558 3641 4383 4468 Size: 182 21 189 179 6 83 742 85 Cuts at: 4468 5539 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 427 417 399 189 182 179 159 90 85 83 53 36 21 9 6 BspEI T'CCGG_A Cuts at: 2074 2902 5985 2074 Size: 828 3083 2411 Fragments arranged by size: 3083 2411 828 BspHI T'CATG_A Cuts at: 4211 5941 4211 Size: 1730 4592 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2073 2905 3520 3677 844 Size: 1229 832 615 157 3489 Fragments arranged by size: 3489 1229 832 615 157 BsrBI CCG'CTC Cuts at: 1394 1823 2125 2220 3103 4209 1394 Size: 429 302 95 883 1106 3507 Fragments arranged by size: 3507 1106 883 429 302 95 BsrDI GCAATG_nn' Cuts at: 65 1248 4817 4991 65 Size: 1183 3569 174 1396 Fragments arranged by size: 3569 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2024 3071 3446 4966 5153 1700 Size: 110 214 1047 375 1520 187 2869 Fragments arranged by size: 2869 1520 1047 375 214 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6322 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6322 BssSI C'ACGA_G Cuts at: 3543 3551 3557 4382 5394 3543 Size: 8 6 825 1012 4471 Fragments arranged by size: 4471 1012 825 8 6 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6322 BstBI TT'CG_AA Cuts at: 5126 5126 Size: 6322 BstEII G'GTnAC_C Cuts at: 1184 3507 1184 Size: 2323 3999 BstXI CCAn_nnnn'nTGG Cuts at: 2188 2863 2188 Size: 675 5647 BstYI r'GATC_y Cuts at: 1972 2071 2265 2760 3062 3652 4019 4404 4421 Size: 99 194 495 302 590 367 385 17 Cuts at: 4421 5873 5959 5971 6317 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 590 495 385 367 346 302 194 99 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2055 3345 2055 Size: 1290 5032 BtgI C'CryG_G Cuts at: 513 1065 2115 3572 3586 513 Size: 552 1050 1457 14 3249 Fragments arranged by size: 3249 1457 1050 552 14 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5583 Fragments arranged by size: 5583 683 56 BtsI GCAGTG_nn' Cuts at: 3838 4632 4652 3838 Size: 794 20 5508 Fragments arranged by size: 5508 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6322 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6287 DraI TTT'AAA Cuts at: 3626 3626 Size: 6322 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5329 809 Size: 507 4013 1802 Fragments arranged by size: 4013 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2533 2775 3033 Size: 54 1425 50 275 18 703 242 258 Cuts at: 3033 3104 3769 4658 6287 8 Size: 71 665 889 1629 43 Fragments arranged by size: 1629 1425 889 703 665 275 258 242 71 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3769 1537 Size: 275 1957 4090 Fragments arranged by size: 4090 1957 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2052 2595 2684 2969 4252 1353 Size: 699 543 89 285 1283 3423 Fragments arranged by size: 3423 1283 699 543 285 89 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2040 3433 4909 5283 5429 387 Size: 1064 138 451 1393 1476 374 146 1280 Fragments arranged by size: 1476 1393 1280 1064 451 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2254 2536 3274 3280 3431 4385 4982 Size: 393 622 282 738 6 151 954 597 Cuts at: 4982 5769 6037 1239 Size: 787 268 1524 Fragments arranged by size: 1524 954 787 738 622 597 393 282 268 151 6 EcoO109I rG'GnC_Cy Cuts at: 2059 2185 2238 2924 3088 3119 3180 3181 3232 Size: 126 53 686 164 31 61 1 51 Cuts at: 3232 3242 3249 3330 3369 3370 2059 Size: 10 7 81 39 1 5011 Fragments arranged by size: 5011 686 164 126 81 61 53 51 39 31 10 7 1 1 EcoRI G'AATT_C Cuts at: 3631 4009 3631 Size: 378 5944 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3475 3475 Size: 6322 FspI TGC'GCA Cuts at: 4828 4828 Size: 6322 HaeII r_GCGC'y Cuts at: 1678 2103 2326 2784 2957 5469 1678 Size: 425 223 458 173 2512 2531 Fragments arranged by size: 2531 2512 458 425 223 173 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2837 2869 3247 3279 4963 Size: 32 135 32 1265 32 378 32 1684 Cuts at: 4963 4995 5037 5069 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1684 1265 378 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3666 3912 669 Size: 988 281 1728 246 3079 Fragments arranged by size: 3079 1728 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6322 HpaI GTT'AAC Cuts at: 3912 3912 Size: 6322 KasI G'GCGC_C Cuts at: 2953 2953 Size: 6322 KpnI G_GTAC'C Cuts at: 3647 3647 Size: 6322 MfeI C'AATT_G Cuts at: 3921 3921 Size: 6322 MluI A'CGCG_T Cuts at: 6198 6198 Size: 6322 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2289 5436 5620 1305 Size: 984 3147 184 2007 Fragments arranged by size: 3147 2007 984 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2535 10 Size: 54 1425 343 703 3797 Fragments arranged by size: 3797 1425 703 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2615 3044 4280 4639 4798 518 Size: 648 1449 429 1236 359 159 2042 Fragments arranged by size: 2042 1449 1236 648 429 359 159 MspA1I CmG'CkG Cuts at: 1606 2232 2643 2758 2934 2960 3339 3738 4415 Size: 626 411 115 176 26 379 399 677 Cuts at: 4415 5563 5808 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 626 411 399 379 245 176 115 26 NaeI GCC'GGC Cuts at: 2026 3073 2026 Size: 1047 5275 NarI GG'CG_CC Cuts at: 2954 2954 Size: 6322 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5770 NdeI CA'TA_TG Cuts at: 387 387 Size: 6322 NgoMIV G'CCGG_C Cuts at: 2024 3071 2024 Size: 1047 5275 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6322 NotI GC'GGCC_GC Cuts at: 3769 3769 Size: 6322 NsiI A_TGCA'T Cuts at: 3047 3047 Size: 6322 NspI r_CATG'y Cuts at: 1132 1229 2470 2624 3045 3680 5225 1132 Size: 97 1241 154 421 635 1545 2229 Fragments arranged by size: 2229 1545 1241 635 421 154 97 PasI CC'CwG_GG Cuts at: 1348 2063 1348 Size: 715 5607 PciI A'CATG_T Cuts at: 5221 5221 Size: 6322 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6322 PfoI T'CCnGG_A Cuts at: 2283 2820 3006 2283 Size: 537 186 5599 Fragments arranged by size: 5599 537 186 PmeI GTTT'AAAC Cuts at: 3626 3626 Size: 6322 PmlI CAC'GTG Cuts at: 2581 2581 Size: 6322 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4391 4423 5904 5936 4391 Size: 32 1481 32 4777 Fragments arranged by size: 4777 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2059 3242 3330 2059 Size: 1183 88 5051 Fragments arranged by size: 5051 1183 88 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6322 PsiI TTA'TAA Cuts at: 3892 3892 Size: 6322 PspOMI G'GGCC_C Cuts at: 2888 2924 3159 3180 3369 2888 Size: 36 235 21 189 5841 Fragments arranged by size: 5841 235 189 36 21 PstI C_TGCA'G Cuts at: 830 2390 3096 3359 3378 3674 830 Size: 1560 706 263 19 296 3478 Fragments arranged by size: 3478 1560 706 296 263 19 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6322 PvuII CAG'CTG Cuts at: 2960 2960 Size: 6322 SalI G'TCGA_C Cuts at: 1936 3664 1936 Size: 1728 4594 SapI GCTCTTCn'nnn_ Cuts at: 2052 2969 2052 Size: 917 5405 SbfI CC_TGCA'GG Cuts at: 3359 3378 3674 3359 Size: 19 296 6007 Fragments arranged by size: 6007 296 19 ScaI AGT'ACT Cuts at: 4570 6282 4570 Size: 1712 4610 SfcI C'TryA_G Cuts at: 826 1045 2386 2572 3092 3355 3374 3670 4805 Size: 219 1341 186 520 263 19 296 1135 Cuts at: 4805 5486 5677 826 Size: 681 191 1471 Fragments arranged by size: 1471 1341 1135 681 520 296 263 219 191 186 19 SfiI GGCCn_nnn'nGGCC Cuts at: 2673 2673 Size: 6322 SfoI GGC'GCC Cuts at: 2955 2955 Size: 6322 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6322 SgrAI Cr'CCGG_yG Cuts at: 1700 3071 1700 Size: 1371 4951 SmaI CCC'GGG Cuts at: 1872 2887 3019 3117 3158 3164 3216 3315 3649 Size: 1015 132 98 41 6 52 99 334 Cuts at: 3649 1872 Size: 4545 Fragments arranged by size: 4545 1015 334 132 99 98 52 41 6 SmlI C'TyrA_G Cuts at: 820 1017 1943 2095 4426 5327 5589 820 Size: 197 926 152 2331 901 262 1553 Fragments arranged by size: 2331 1553 926 901 262 197 152 SnaBI TAC'GTA Cuts at: 493 4113 493 Size: 3620 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6322 SphI G_CATG'C Cuts at: 1132 3680 1132 Size: 2548 3774 SrfI GCCC'GGGC Cuts at: 3164 3315 3164 Size: 151 6171 SspI AAT'ATT Cuts at: 5 52 4246 5 Size: 47 4194 2081 Fragments arranged by size: 4194 2081 47 StuI AGG'CCT Cuts at: 1517 2606 2676 1517 Size: 1089 70 5163 Fragments arranged by size: 5163 1089 70 StyI C'CwwG_G Cuts at: 513 1065 2181 2856 3237 3602 3761 513 Size: 552 1116 675 381 365 159 3074 Fragments arranged by size: 3074 1116 675 552 381 365 159 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2123 3124 4349 4366 4519 4704 5149 Size: 1255 543 1001 1225 17 153 185 445 Cuts at: 5149 325 Size: 1498 Fragments arranged by size: 1498 1255 1225 1001 543 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4568 6280 96 Size: 274 80 33 51 1443 2591 1712 138 Fragments arranged by size: 2591 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2069 2101 6282 6314 2069 Size: 32 4181 32 2077 Fragments arranged by size: 4181 2077 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3535 1316 Size: 2219 4103 XbaI T'CTAG_A Cuts at: 3658 3658 Size: 6322 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2513 2513 Size: 6322 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6322 XmaI C'CCGG_G Cuts at: 1870 2885 3017 3115 3156 3162 3214 3313 3647 Size: 1015 132 98 41 6 52 99 334 Cuts at: 3647 1870 Size: 4545 Fragments arranged by size: 4545 1015 334 132 99 98 52 41 6 XmnI GAAnn'nnTTC Cuts at: 4451 4451 Size: 6322 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4130 276 Size: 53 83 186 936 2596 2468 Fragments arranged by size: 2596 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BbvCI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI NruI PacI PspXI PsrI RsrII SacI SacII SanDI SexAI SwaI