MIER1 ORF clone pFN21AE1157

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE1157

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Clone info.

Species

human

Clone Name

pFN21AE1157 Sequence (with vector) Restriction Map

Product ID

FHC10824

ORF Sequence

DNA sequence (1695 bp)
Protein sequence (565 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB590466]

Gene

KIEE1157

Symbol

MIER1

Alias : ER1, MI-ER1

Description

mesoderm induction early response 1, transcriptional regulator, transcript variant 2

Original Clone ID

cs03030

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1695 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 565 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS53326.1 5.6e-140 100.0 MIER1 CCDS41348.1 3.3e-125 100.0 MIER1 CCDS53329.1 3.4e-125 100.0 MIER1 CCDS53325.1 3e-114 100.0 MIER1 CCDS53328.1 2.1e-104 100.0 MIER1 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR000949 235 287 PF01448 ELM2 domain HMMSmart IPR001005 337 386 SM00717 SANT domain ProfileScan IPR000949 233 331 PS51156 ELM2 domain IPR017884 336 388 PS51293 SANT
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_001071168 565 616848 100.0 100.0 XP_016857416 511 616848 100.0 90.4 XP_016857417 511 616848 100.0 90.4 NP_001071169 512 616848 100.0 90.1 XP_016857413 529 616848 100.0 90.1 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE1157 Download

>KIEE1157  1695 bp
ATGGATGGGGCTTCTTCAGGCGGTGGCGGCAGCAGCGAAGGTGGCGGCGGCAGCAGCGGC
AGCGGCTATGGTGTGGTCGCTCGATTCTCCCAGTGCCTGGCTGAGTTTCGGACGTGGTTA
AGAACCAACTGGTTGAGGTTCAATGCAGACAAGACGGATGTGATGCTGCCATCTGTTGAA
TCTTCAAGTCCAGGAGGTTCAGCAACATCAGATGACCATGAATTTGATCCATCAGCTGAC
ATGCTGGTTCATGATTTTGATGATGAACGAACATTAGAAGAGGAAGAAATGATGGAAGGA
GAAACAAACTTCAGCTCTGAAATAGAAGATCTTGCAAGGGAAGGCGACATGCCAATTCAT
GAACTTCTCAGCCTTTATGGTTATGGTAGTACTGTTCGACTACCTGAAGAAGATGAGGAA
GAGGAAGAAGAGGAAGAAGAAGGTGAAGATGATGAAGATGCTGATAATGATGACAACAGT
GGCTGTAGTGGGGAAAATAAAGAGGAGAATATAAAGGATTCATCAGGTCAGGAGGATGAA
ACTCAGTCTTCCAATGATGATCCATCACAATCTGTTGCTTCTCAAGATGCCCAGGAAATA
ATCCGCCCACGTCGATGTAAATATTTTGATACAAATAGTGAAGTAGAAGAAGAATCTGAA
GAAGATGAAGATTATATTCCATCAGAAGACTGGAAAAAGGAGATTATGGTGGGCTCCATG
TTTCAAGCAGAAATTCCAGTTGGCATTTGTAGATACAAAGAAAATGAAAAAGTATATGAA
AATGATGATCAGCTCCTGTGGGACCCTGAGTACTTACCAGAAGATAAAGTGATTATATTT
CTTAAAGATGCATCTAGAAGAACAGGTGATGAGAAGGGTGTAGAAGCAATTCCTGAAGGA
TCTCACATAAAAGACAATGAACAGGCTTTATATGAATTGGTTAAATGCAATTTTGATACA
GAAGAAGCATTGAGAAGATTAAGATTTAATGTAAAAGCAGCTAGAGAGGAATTATCTGTT
TGGACAGAGGAAGAGTGTAGAAATTTTGAACAAGGGCTGAAGGCCTATGGAAAGGATTTT
CATTTGATTCAGGCTAATAAAGTCCGAACAAGGTCAGTTGGTGAATGTGTAGCATTCTAT
TACATGTGGAAAAAATCTGAACGTTATGATTTCTTTGCTCAGCAAACACGATTTGGAAAG
AAGAAATATAATCTTCATCCTGGTGTAACGGATTACATGGATCGTCTTCTAGACGAAAGT
GAAAGTGCTGCATCTAGTCGAGCACCATCCCCTCCCCCAACTGCATCAAACAGTAGTAAC
AGCCAGTCTGAGAAAGAAGATGGCACTGTAAGCACTGCTAATCAAAATGGAGTGTCATCT
AATGGACCAGGTGAAATATTAAACAAAGAGGAAGTAAAAGTTGAAGGGTTACACATTAAT
GGACCAACAGGTGGAAATAAGAAACCACTTCATGCAGATATGGATACTAATGGTTATGAA
ACAGATAACCTTACCACTGACCCAAAACTTGCCCATATGACTGCAAGAAATGAAAATGAT
TTTGATGAAAAAAGTGAGAGACCTGCCAAAAGGCGAAGGGTAAACAGCAATGGAAAAGAA
AGTCCAGGTTCTTCTGAATTTTTCCAAGAAGCAGTCTCACATGGGAAATTTGAAGAACTT
GAAAACACAGATGAC

ORF Protein

Cloned ORF protein sequence for pFN21AE1157 Download

>KIEE1157  565 aa
MDGASSGGGGSSEGGGGSSGSGYGVVARFSQCLAEFRTWLRTNWLRFNADKTDVMLPSVE
SSSPGGSATSDDHEFDPSADMLVHDFDDERTLEEEEMMEGETNFSSEIEDLAREGDMPIH
ELLSLYGYGSTVRLPEEDEEEEEEEEEGEDDEDADNDDNSGCSGENKEENIKDSSGQEDE
TQSSNDDPSQSVASQDAQEIIRPRRCKYFDTNSEVEEESEEDEDYIPSEDWKKEIMVGSM
FQAEIPVGICRYKENEKVYENDDQLLWDPEYLPEDKVIIFLKDASRRTGDEKGVEAIPEG
SHIKDNEQALYELVKCNFDTEEALRRLRFNVKAAREELSVWTEEECRNFEQGLKAYGKDF
HLIQANKVRTRSVGECVAFYYMWKKSERYDFFAQQTRFGKKKYNLHPGVTDYMDRLLDES
ESAASSRAPSPPPTASNSSNSQSEKEDGTVSTANQNGVSSNGPGEILNKEEVKVEGLHIN
GPTGGNKKPLHADMDTNGYETDNLTTDPKLAHMTARNENDFDEKSERPAKRRRVNSNGKE
SPGSSEFFQEAVSHGKFEELENTDD

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE1157 Download

>pFN21AE1157  6400 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGATGGGGCTTCTTCAGGCGGTGGCGGCAGCAG
CGAAGGTGGCGGCGGCAGCAGCGGCAGCGGCTATGGTGTGGTCGCTCGATTCTCCCAGTG
CCTGGCTGAGTTTCGGACGTGGTTAAGAACCAACTGGTTGAGGTTCAATGCAGACAAGAC
GGATGTGATGCTGCCATCTGTTGAATCTTCAAGTCCAGGAGGTTCAGCAACATCAGATGA
CCATGAATTTGATCCATCAGCTGACATGCTGGTTCATGATTTTGATGATGAACGAACATT
AGAAGAGGAAGAAATGATGGAAGGAGAAACAAACTTCAGCTCTGAAATAGAAGATCTTGC
AAGGGAAGGCGACATGCCAATTCATGAACTTCTCAGCCTTTATGGTTATGGTAGTACTGT
TCGACTACCTGAAGAAGATGAGGAAGAGGAAGAAGAGGAAGAAGAAGGTGAAGATGATGA
AGATGCTGATAATGATGACAACAGTGGCTGTAGTGGGGAAAATAAAGAGGAGAATATAAA
GGATTCATCAGGTCAGGAGGATGAAACTCAGTCTTCCAATGATGATCCATCACAATCTGT
TGCTTCTCAAGATGCCCAGGAAATAATCCGCCCACGTCGATGTAAATATTTTGATACAAA
TAGTGAAGTAGAAGAAGAATCTGAAGAAGATGAAGATTATATTCCATCAGAAGACTGGAA
AAAGGAGATTATGGTGGGCTCCATGTTTCAAGCAGAAATTCCAGTTGGCATTTGTAGATA
CAAAGAAAATGAAAAAGTATATGAAAATGATGATCAGCTCCTGTGGGACCCTGAGTACTT
ACCAGAAGATAAAGTGATTATATTTCTTAAAGATGCATCTAGAAGAACAGGTGATGAGAA
GGGTGTAGAAGCAATTCCTGAAGGATCTCACATAAAAGACAATGAACAGGCTTTATATGA
ATTGGTTAAATGCAATTTTGATACAGAAGAAGCATTGAGAAGATTAAGATTTAATGTAAA
AGCAGCTAGAGAGGAATTATCTGTTTGGACAGAGGAAGAGTGTAGAAATTTTGAACAAGG
GCTGAAGGCCTATGGAAAGGATTTTCATTTGATTCAGGCTAATAAAGTCCGAACAAGGTC
AGTTGGTGAATGTGTAGCATTCTATTACATGTGGAAAAAATCTGAACGTTATGATTTCTT
TGCTCAGCAAACACGATTTGGAAAGAAGAAATATAATCTTCATCCTGGTGTAACGGATTA
CATGGATCGTCTTCTAGACGAAAGTGAAAGTGCTGCATCTAGTCGAGCACCATCCCCTCC
CCCAACTGCATCAAACAGTAGTAACAGCCAGTCTGAGAAAGAAGATGGCACTGTAAGCAC
TGCTAATCAAAATGGAGTGTCATCTAATGGACCAGGTGAAATATTAAACAAAGAGGAAGT
AAAAGTTGAAGGGTTACACATTAATGGACCAACAGGTGGAAATAAGAAACCACTTCATGC
AGATATGGATACTAATGGTTATGAAACAGATAACCTTACCACTGACCCAAAACTTGCCCA
TATGACTGCAAGAAATGAAAATGATTTTGATGAAAAAAGTGAGAGACCTGCCAAAAGGCG
AAGGGTAAACAGCAATGGAAAAGAAAGTCCAGGTTCTTCTGAATTTTTCCAAGAAGCAGT
CTCACATGGGAAATTTGAAGAACTTGAAAACACAGATGACGTTTAAACGAATTCGGGCTC
GGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACA
AAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCC
TTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACA
ACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTT
GTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTT
CAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGT
AAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAG
TAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTT
TCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTT
TTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAA
TAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTT
TTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGAT
GCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAG
ATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTG
CTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATA
CACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGAT
GGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCC
AACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATG
GGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAAC
GACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACT
GGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAA
GTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCT
GGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCC
TCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGA
CAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAA
GCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAG
GGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAA
AGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATC
GACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCC
CTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCG
CCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTT
CGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACC
GCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGC
CACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAG
AGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCG
CTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAA
CCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAG
GATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACT
CACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAT
AGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAA
AAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAG
GTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTG
CCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATT
AAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGT
ACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT