(Circular) (Six-base) MAPSORT of: pFN21AE1157.seq Check: 3481 from: 1 to: 6400 pFN21AE1157 6400 bp With 182 enzymes: SgfI * August 16, 2010 14:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4210 278 Size: 53 83 186 936 2674 2468 Fragments arranged by size: 2674 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3743 1937 Size: 1806 4594 Acc65I G'GTAC_C Cuts at: 3721 3721 Size: 6400 AclI AA'CG_TT Cuts at: 1563 3166 4527 4900 1563 Size: 1603 1361 373 3063 Fragments arranged by size: 3063 1603 1361 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2004 2299 2430 2682 2919 3083 4463 5847 Size: 372 295 131 252 237 164 1380 1384 Cuts at: 5847 6115 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 1380 372 295 268 252 237 164 131 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6203 AflIII A'CryG_T Cuts at: 1203 3147 5299 6276 1203 Size: 1944 2152 977 1327 Fragments arranged by size: 2152 1944 1327 977 AgeI A'CCGG_T Cuts at: 5231 5231 Size: 6400 AhdI GACnn_n'nnGTC Cuts at: 5129 5129 Size: 6400 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6400 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6400 AlwNI CAG_nnn'CTG Cuts at: 2101 2487 2800 4143 5715 2101 Size: 386 313 1343 1572 2786 Fragments arranged by size: 2786 1572 1343 386 313 ApaLI G'TGCA_C Cuts at: 4457 5613 4457 Size: 1156 5244 ApoI r'AATT_y Cuts at: 1493 2225 2736 3046 3641 3671 3709 3935 4087 Size: 732 511 310 595 30 38 226 152 Cuts at: 4087 6196 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 732 595 511 310 226 152 38 30 AseI AT'TA_AT Cuts at: 160 1032 3441 4954 160 Size: 872 2409 1513 1606 Fragments arranged by size: 2409 1606 1513 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6400 AvaI C'yCGr_G Cuts at: 1870 1943 3725 1870 Size: 73 1782 4545 Fragments arranged by size: 4545 1782 73 BamHI G'GATC_C Cuts at: 3730 4097 3730 Size: 367 6033 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3721 5176 618 Size: 325 217 627 1934 1455 1842 Fragments arranged by size: 1934 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2720 3719 1391 Size: 1329 999 4072 Fragments arranged by size: 4072 1329 999 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2544 2697 3242 928 Size: 971 645 153 545 4086 Fragments arranged by size: 4086 971 645 545 153 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4591 4625 1903 Size: 34 2654 34 3678 Fragments arranged by size: 3678 2654 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3481 4292 5502 3481 Size: 811 1210 4379 Fragments arranged by size: 4379 1210 811 BclI T'GATC_A Cuts at: 1548 2791 1548 Size: 1243 5157 BfrBI ATG'CAT Cuts at: 2855 2855 Size: 6400 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5011 136 Size: 107 122 71 631 605 3339 1525 Fragments arranged by size: 3339 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2332 6395 2332 Size: 4063 2337 BlpI GC'TnA_GC Cuts at: 3183 3817 3183 Size: 634 5766 Bme1580I G_kGCm'C Cuts at: 1790 4461 5617 1790 Size: 2671 1156 2573 Fragments arranged by size: 2671 2573 1156 BmgBI CAC'GTC Cuts at: 1318 2118 2615 1318 Size: 800 497 5103 Fragments arranged by size: 5103 800 497 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2089 4131 5089 Size: 416 53 83 399 24 672 2042 958 Cuts at: 5089 442 Size: 1753 Fragments arranged by size: 2042 1753 958 672 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6400 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 5060 1239 Size: 3821 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2571 4525 5390 5688 2571 Size: 1954 865 298 3283 Fragments arranged by size: 3283 1954 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3577 5063 882 Size: 617 2078 1486 2219 Fragments arranged by size: 2219 2078 1486 617 BsaAI yAC'GTr Cuts at: 493 1204 4191 493 Size: 711 2987 2702 Fragments arranged by size: 2987 2702 711 BsaBI GATnn'nnATC Cuts at: 6394 6394 Size: 6400 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4207 4589 5224 275 Size: 53 83 186 936 2674 382 635 1451 Fragments arranged by size: 2674 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4833 5231 5505 5652 6063 4833 Size: 398 274 147 411 5170 Fragments arranged by size: 5170 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6175 Fragments arranged by size: 6175 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2522 1186 Size: 1336 5064 BseYI C'CCAG_C Cuts at: 5603 6099 5603 Size: 496 5904 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5827 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3850 4611 5639 1540 Size: 275 186 1849 761 1028 2301 Fragments arranged by size: 2301 1849 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3289 4461 4546 5617 3289 Size: 1172 85 1071 4072 Fragments arranged by size: 4072 1172 1071 85 BsmI GAATG_Cn' Cuts at: 3137 3911 4004 3137 Size: 774 93 5533 Fragments arranged by size: 5533 774 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2720 3289 3719 4461 4546 5617 1391 Size: 399 930 569 430 742 85 1071 2174 Fragments arranged by size: 2174 1071 930 742 569 430 399 85 BspEI T'CCGG_A Cuts at: 6063 6063 Size: 6400 BspHI T'CATG_A Cuts at: 2254 2362 4289 6019 2254 Size: 108 1927 1730 2635 Fragments arranged by size: 2635 1927 1730 108 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3595 3755 844 Size: 2751 160 3489 Fragments arranged by size: 3489 2751 160 BsrBI CCG'CTC Cuts at: 1394 1823 4287 1394 Size: 429 2464 3507 Fragments arranged by size: 3507 2464 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3619 4895 5069 65 Size: 1183 2371 1276 174 1396 Fragments arranged by size: 2371 1396 1276 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5044 5231 1700 Size: 110 3234 187 2869 Fragments arranged by size: 3234 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6400 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6400 BssSI C'ACGA_G Cuts at: 4460 5472 4460 Size: 1012 5388 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6400 BstBI TT'CG_AA Cuts at: 5204 5204 Size: 6400 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6400 BstYI r'GATC_y Cuts at: 1972 2332 2903 3730 4097 4482 4499 5951 6037 Size: 360 571 827 367 385 17 1452 86 Cuts at: 6037 6049 6395 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 827 571 385 367 360 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4909 Fragments arranged by size: 4909 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5661 Fragments arranged by size: 5661 683 56 BtsI GCAGTG_nn' Cuts at: 3357 3916 4710 4730 3357 Size: 559 794 20 5027 Fragments arranged by size: 5027 794 559 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6400 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6365 DraI TTT'AAA Cuts at: 3704 3704 Size: 6400 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5407 809 Size: 507 4091 1802 Fragments arranged by size: 4091 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3847 4736 6365 Size: 54 1425 50 275 18 2017 889 1629 Cuts at: 6365 8 Size: 43 Fragments arranged by size: 2017 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3847 1537 Size: 275 2035 4090 Fragments arranged by size: 4090 2035 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2277 2418 2427 3030 4330 1353 Size: 924 141 9 603 1300 3423 Fragments arranged by size: 3423 1300 924 603 141 9 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2597 4987 5361 5507 387 Size: 1064 138 1008 2390 374 146 1280 Fragments arranged by size: 2390 1280 1064 1008 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2004 2299 2430 2682 2919 3083 4463 Size: 393 372 295 131 252 237 164 1380 Cuts at: 4463 5060 5847 6115 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1380 787 597 393 372 295 268 252 237 164 131 EcoO109I rG'GnC_Cy Cuts at: 2806 2806 Size: 6400 EcoRI G'AATT_C Cuts at: 3709 4087 3709 Size: 378 6022 FspI TGC'GCA Cuts at: 4906 4906 Size: 6400 HaeII r_GCGC'y Cuts at: 1678 5547 1678 Size: 3869 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2552 2584 3002 3034 5041 Size: 32 135 32 980 32 418 32 2007 Cuts at: 5041 5073 5115 5147 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2007 980 418 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3744 3990 669 Size: 988 281 1806 246 3079 Fragments arranged by size: 3079 1806 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6400 HpaI GTT'AAC Cuts at: 3990 3990 Size: 6400 KpnI G_GTAC'C Cuts at: 3725 3725 Size: 6400 MfeI C'AATT_G Cuts at: 3999 3999 Size: 6400 MluI A'CGCG_T Cuts at: 6276 6276 Size: 6400 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5514 5698 1305 Size: 4209 184 2007 Fragments arranged by size: 4209 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4578 Fragments arranged by size: 4578 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2617 3225 3441 4358 4717 4876 518 Size: 648 1451 608 216 917 359 159 2042 Fragments arranged by size: 2042 1451 917 648 608 359 216 159 MspA1I CmG'CkG Cuts at: 1606 2061 2067 2240 3816 4493 5641 5886 1606 Size: 455 6 173 1576 677 1148 245 2120 Fragments arranged by size: 2120 1576 1148 677 455 245 173 6 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4909 Fragments arranged by size: 4909 939 552 NdeI CA'TA_TG Cuts at: 387 3540 387 Size: 3153 3247 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6400 NotI GC'GGCC_GC Cuts at: 3847 3847 Size: 6400 NsiI A_TGCA'T Cuts at: 2857 2857 Size: 6400 NspI r_CATG'y Cuts at: 1132 1229 2248 2356 3151 3758 5303 1132 Size: 97 1019 108 795 607 1545 2229 Fragments arranged by size: 2229 1545 1019 795 607 108 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6400 PciI A'CATG_T Cuts at: 3147 5299 3147 Size: 2152 4248 PflMI CCAn_nnn'nTGG Cuts at: 1108 2101 3455 1108 Size: 993 1354 4053 Fragments arranged by size: 4053 1354 993 PfoI T'CCnGG_A Cuts at: 2194 2194 Size: 6400 PmeI GTTT'AAAC Cuts at: 3704 3704 Size: 6400 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4469 4501 5982 6014 4469 Size: 32 1481 32 4855 Fragments arranged by size: 4855 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2806 2806 Size: 6400 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6400 PsiI TTA'TAA Cuts at: 3970 3970 Size: 6400 PstI C_TGCA'G Cuts at: 830 3752 830 Size: 2922 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6400 PvuII CAG'CTG Cuts at: 2240 2240 Size: 6400 SalI G'TCGA_C Cuts at: 1936 3742 1936 Size: 1806 4594 SanDI GG'GwC_CC Cuts at: 2806 2806 Size: 6400 SbfI CC_TGCA'GG Cuts at: 3752 3752 Size: 6400 ScaI AGT'ACT Cuts at: 2395 2816 4648 6360 2395 Size: 421 1832 1712 2435 Fragments arranged by size: 2435 1832 1712 421 SexAI A'CCwGG_T Cuts at: 3391 3391 Size: 6400 SfcI C'TryA_G Cuts at: 826 1045 2488 3748 4883 5564 5755 826 Size: 219 1443 1260 1135 681 191 1471 Fragments arranged by size: 1471 1443 1260 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6400 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6400 SmaI CCC'GGG Cuts at: 1872 3727 1872 Size: 1855 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2586 4504 5405 5667 820 Size: 197 926 643 1918 901 262 1553 Fragments arranged by size: 1918 1553 926 901 643 262 197 SnaBI TAC'GTA Cuts at: 493 4191 493 Size: 3698 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6400 SphI G_CATG'C Cuts at: 1132 3758 1132 Size: 2626 3774 SspI AAT'ATT Cuts at: 5 52 2627 3402 4324 5 Size: 47 2575 775 922 2081 Fragments arranged by size: 2575 2081 922 775 47 StuI AGG'CCT Cuts at: 1517 3068 1517 Size: 1551 4849 StyI C'CwwG_G Cuts at: 513 1065 2004 3839 513 Size: 552 939 1835 3074 Fragments arranged by size: 3074 1835 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4427 4444 4597 4782 5227 325 Size: 1255 2847 17 153 185 445 1498 Fragments arranged by size: 2847 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2393 2814 4646 Size: 274 80 33 51 1443 416 421 1832 Cuts at: 4646 6358 96 Size: 1712 138 Fragments arranged by size: 1832 1712 1443 421 416 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6360 6392 6360 Size: 32 6368 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6400 XbaI T'CTAG_A Cuts at: 2858 3253 3736 2858 Size: 395 483 5522 Fragments arranged by size: 5522 483 395 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2691 2691 Size: 6400 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6400 XmaI C'CCGG_G Cuts at: 1870 3725 1870 Size: 1855 4545 XmnI GAAnn'nnTTC Cuts at: 2892 4529 2892 Size: 1637 4763 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4208 276 Size: 53 83 186 936 2674 2468 Fragments arranged by size: 2674 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI SfiI SfoI SrfI SwaI