GJA9 ORF clone pFN21AB9874

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AB9874

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Clone info.

Species

human

Clone Name

pFN21AB9874 Sequence (with vector) Restriction Map

Product ID

FHC03376

Flexi Clone PID

FXC03376

ORF Sequence

DNA sequence (1545 bp)
Protein sequence (515 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB464552]

Gene

KIBB9874

Symbol

GJA9

Alias : CX58, CX59, GJA10

Description

gap junction protein, alpha 9, 59kDa

Original Clone ID

cp00443

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1545 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 515 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS432.1 6.4e-207 100.0 GJA9 CCDS5025.1 1.9e-67 47.7 GJA10 CCDS9289.1 1.7e-51 54.5 GJA3 CCDS30834.1 3e-51 39.8 GJA8 CCDS929.1 3.8e-49 45.9 GJA5 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR000500 21 45 PR00206 Connexin IPR000500 52 74 PR00206 Connexin IPR000500 77 97 PR00206 Connexin IPR000500 153 179 PR00206 Connexin IPR000500 189 209 PR00206 Connexin IPR000500 210 233 PR00206 Connexin HMMPfam IPR013092 3 107 PF00029 Connexin IPR019570 167 233 PF10582 Gap junction protein HMMSmart IPR013092 43 76 SM00037 Connexin
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 22 GKIWLTILFIFRMLVLGVAAEDV 44 PRIMARY 23 2 74 LIRYWVLQVIFVSSPSLVYMGHA 96 PRIMARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_110399 515 611923 100.0 100.0 NP_115991 543 611924 47.7 88.0 XP_011507719 427 116200,600897,612474 39.8 80.2 NP_005258 433 116200,600897,612474 39.8 80.2 XP_011507718 433 116200,600897,612474 39.8 80.2 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB9874 Download

>KIBB9874  1545 bp
ATGGGGGACTGGAATCTCCTTGGAGATACTCTGGAGGAAGTTCACATCCACTCCACCATG
ATTGGAAAGATCTGGCTCACCATCCTGTTCATATTTCGAATGCTTGTTCTGGGTGTAGCA
GCTGAAGATGTCTGGAATGATGAGCAGTCTGGCTTCATCTGCAATACAGAACAACCAGGC
TGCAGAAATGTATGCTACGACCAGGCCTTTCCTATCTCCCTCATTAGATACTGGGTTCTG
CAGGTGATATTTGTGTCTTCACCATCCCTGGTCTACATGGGCCATGCATTGTACCGACTG
AGAGTTCTTGAGGAAGAGAGGCAAAGGATGAAAGCTCAGTTAAGAGTAGAACTGGAGGAG
GTAGAGTTTGAAATGCCTAGGGATCGGAGGAGATTGGAGCAAGAGCTTTGTCAGCTGGAG
AAAAGGAAACTAAATAAAGCTCCACTCAGAGGAACCTTGCTTTGCACTTATGTGATACAC
ATTTTCACTCGCTCTGTGGTTGAAGTTGGATTCATGATTGGACAGTACCTTTTATATGGA
TTTCACTTAGAGCCGCTATTTAAGTGCCATGGCCACCCGTGTCCAAATATAATCGACTGT
TTTGTCTCAAGACCAACAGAAAAGACAATATTCCTATTATTTATGCAATCTATAGCCACT
ATTTCACTTTTCTTAAACATTCTTGAAATTTTCCACCTAGGTTTTAAAAAGATTAAAAGA
GGGCTTTGGGGAAAATACAAGTTGAAGAAGGAACATAATGAATTCCATGCAAACAAGGCA
AAACAAAATGTAGCCAAATACCAGAGCACATCTGCAAATTCACTGAAGCGACTCCCTTCT
GCCCCTGATTATAATCTGTTAGTGGAAAAGCAAACACACACTGCAGTGTACCCTAGTTTA
AATTCATCTTCTGTATTCCAGCCAAATCCTGACAATCATAGTGTAAATGATGAGAAATGC
ATTTTGGATGAACAGGAAACTGTACTTTCTAATGAGATTTCCACACTTAGTACTAGTTGT
AGTCATTTTCAACACATCAGTTCAAACAATAACAAAGACACTCATAAAATATTTGGAAAA
GAACTTAATGGTAACCAGTTAATGGAAAAAAGAGAAACTGAAGGCAAAGACAGCAAAAGG
AACTACTACTCTAGAGGTCACCGCTCTATTCCAGGTGTTGCTATAGATGGAGAGAACAAC
ATGAGGCAGTCACCCCAAACAGTTTTCTCCTTGCCAGCTAACTGCGATTGGAAACCGCGG
TGGCTTAGAGCTACATGGGGTTCCTCTACAGAACATGAAAACCGGGGGTCACCTCCTAAA
GGTAACCTCAAGGGCCAGTTCAGAAAGGGCACAGTCAGAACCCTTCCTCCTTCACAAGGA
GATTCTCAATCACTTGACATTCCAAACACTGCTGATTCTTTGGGAGGGCTGTCCTTTGAG
CCAGGGTTGGTCAGAACCTGTAATAATCCTGTTTGTCCTCCAAATCACGTAGTGTCCCTA
ACGAACAATCTCATTGGTAGGCGGGTTCCCACAGATCTTCAGATC

ORF Protein

Cloned ORF protein sequence for pFN21AB9874 Download

>KIBB9874  515 aa
MGDWNLLGDTLEEVHIHSTMIGKIWLTILFIFRMLVLGVAAEDVWNDEQSGFICNTEQPG
CRNVCYDQAFPISLIRYWVLQVIFVSSPSLVYMGHALYRLRVLEEERQRMKAQLRVELEE
VEFEMPRDRRRLEQELCQLEKRKLNKAPLRGTLLCTYVIHIFTRSVVEVGFMIGQYLLYG
FHLEPLFKCHGHPCPNIIDCFVSRPTEKTIFLLFMQSIATISLFLNILEIFHLGFKKIKR
GLWGKYKLKKEHNEFHANKAKQNVAKYQSTSANSLKRLPSAPDYNLLVEKQTHTAVYPSL
NSSSVFQPNPDNHSVNDEKCILDEQETVLSNEISTLSTSCSHFQHISSNNNKDTHKIFGK
ELNGNQLMEKRETEGKDSKRNYYSRGHRSIPGVAIDGENNMRQSPQTVFSLPANCDWKPR
WLRATWGSSTEHENRGSPPKGNLKGQFRKGTVRTLPPSQGDSQSLDIPNTADSLGGLSFE
PGLVRTCNNPVCPPNHVVSLTNNLIGRRVPTDLQI

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB9874 Download

>pFN21AB9874  6250 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGGGGACTGGAATCTCCTTGGAGATACTCTGGA
GGAAGTTCACATCCACTCCACCATGATTGGAAAGATCTGGCTCACCATCCTGTTCATATT
TCGAATGCTTGTTCTGGGTGTAGCAGCTGAAGATGTCTGGAATGATGAGCAGTCTGGCTT
CATCTGCAATACAGAACAACCAGGCTGCAGAAATGTATGCTACGACCAGGCCTTTCCTAT
CTCCCTCATTAGATACTGGGTTCTGCAGGTGATATTTGTGTCTTCACCATCCCTGGTCTA
CATGGGCCATGCATTGTACCGACTGAGAGTTCTTGAGGAAGAGAGGCAAAGGATGAAAGC
TCAGTTAAGAGTAGAACTGGAGGAGGTAGAGTTTGAAATGCCTAGGGATCGGAGGAGATT
GGAGCAAGAGCTTTGTCAGCTGGAGAAAAGGAAACTAAATAAAGCTCCACTCAGAGGAAC
CTTGCTTTGCACTTATGTGATACACATTTTCACTCGCTCTGTGGTTGAAGTTGGATTCAT
GATTGGACAGTACCTTTTATATGGATTTCACTTAGAGCCGCTATTTAAGTGCCATGGCCA
CCCGTGTCCAAATATAATCGACTGTTTTGTCTCAAGACCAACAGAAAAGACAATATTCCT
ATTATTTATGCAATCTATAGCCACTATTTCACTTTTCTTAAACATTCTTGAAATTTTCCA
CCTAGGTTTTAAAAAGATTAAAAGAGGGCTTTGGGGAAAATACAAGTTGAAGAAGGAACA
TAATGAATTCCATGCAAACAAGGCAAAACAAAATGTAGCCAAATACCAGAGCACATCTGC
AAATTCACTGAAGCGACTCCCTTCTGCCCCTGATTATAATCTGTTAGTGGAAAAGCAAAC
ACACACTGCAGTGTACCCTAGTTTAAATTCATCTTCTGTATTCCAGCCAAATCCTGACAA
TCATAGTGTAAATGATGAGAAATGCATTTTGGATGAACAGGAAACTGTACTTTCTAATGA
GATTTCCACACTTAGTACTAGTTGTAGTCATTTTCAACACATCAGTTCAAACAATAACAA
AGACACTCATAAAATATTTGGAAAAGAACTTAATGGTAACCAGTTAATGGAAAAAAGAGA
AACTGAAGGCAAAGACAGCAAAAGGAACTACTACTCTAGAGGTCACCGCTCTATTCCAGG
TGTTGCTATAGATGGAGAGAACAACATGAGGCAGTCACCCCAAACAGTTTTCTCCTTGCC
AGCTAACTGCGATTGGAAACCGCGGTGGCTTAGAGCTACATGGGGTTCCTCTACAGAACA
TGAAAACCGGGGGTCACCTCCTAAAGGTAACCTCAAGGGCCAGTTCAGAAAGGGCACAGT
CAGAACCCTTCCTCCTTCACAAGGAGATTCTCAATCACTTGACATTCCAAACACTGCTGA
TTCTTTGGGAGGGCTGTCCTTTGAGCCAGGGTTGGTCAGAACCTGTAATAATCCTGTTTG
TCCTCCAAATCACGTAGTGTCCCTAACGAACAATCTCATTGGTAGGCGGGTTCCCACAGA
TCTTCAGATCGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTG
CAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTG
CCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATA
AGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATT
TGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTT
AACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTT
TTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCG
CTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGT
TTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGA
AATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTC
ATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATT
CAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCT
CACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGT
TACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGT
TTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGAC
GCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTAC
TCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCT
GCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCG
AAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGG
GAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCA
ATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAA
CAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTT
CCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATC
ATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGG
AGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATT
AAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAA
AGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAA
AAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGC
TCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGA
CAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTC
CGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTT
CTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCT
GTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTG
AGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTA
GCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCT
ACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAA
GAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTT
GCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTA
CGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTAT
CAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGAT
GGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGG
TAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGC
GATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGC
GCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCT
GCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGG
ATCGAGATCT