(Circular) (Six-base) MAPSORT of: pFN21AB9874.seq Check: 8931 from: 1 to: 6250 pFN21AB9874 6250 bp With 182 enzymes: SgfI * February 27, 2009 14:45 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2236 2236 Size: 6250 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4060 278 Size: 53 83 186 936 2524 2468 Fragments arranged by size: 2524 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2277 3593 1937 Size: 340 1316 4594 Fragments arranged by size: 4594 1316 340 Acc65I G'GTAC_C Cuts at: 3571 3571 Size: 6250 AclI AA'CG_TT Cuts at: 1563 4377 4750 1563 Size: 2814 373 3063 Fragments arranged by size: 3063 2814 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2148 2849 3144 3527 4313 5697 5965 1632 Size: 516 701 295 383 786 1384 268 1917 Fragments arranged by size: 1917 1384 786 701 516 383 295 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6053 AflIII A'CryG_T Cuts at: 1203 5149 6126 1203 Size: 3946 977 1327 Fragments arranged by size: 3946 1327 977 AgeI A'CCGG_T Cuts at: 5081 5081 Size: 6250 AhdI GACnn_n'nnGTC Cuts at: 4979 4979 Size: 6250 AleI CACnn'nnGTG Cuts at: 1166 2474 2888 1166 Size: 1308 414 4528 Fragments arranged by size: 4528 1308 414 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6250 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3355 3387 3355 Size: 32 6218 AlwNI CAG_nnn'CTG Cuts at: 3462 3993 5565 3462 Size: 531 1572 4147 Fragments arranged by size: 4147 1572 531 ApaLI G'TGCA_C Cuts at: 4307 5463 4307 Size: 1156 5094 ApoI r'AATT_y Cuts at: 1493 2691 2765 2821 2905 3559 3785 3937 6046 Size: 1198 74 56 84 654 226 152 2109 Cuts at: 6046 1493 Size: 1697 Fragments arranged by size: 2109 1697 1198 654 226 152 84 74 56 AseI AT'TA_AT Cuts at: 160 1032 4804 160 Size: 872 3772 1606 Fragments arranged by size: 3772 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6250 AvaI C'yCGr_G Cuts at: 1870 1943 3575 1870 Size: 73 1632 4545 Fragments arranged by size: 4545 1632 73 AvrII C'CTAG_G Cuts at: 2381 2701 2381 Size: 320 5930 BamHI G'GATC_C Cuts at: 3580 3947 3580 Size: 367 5883 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3571 5026 618 Size: 325 217 627 1784 1455 1842 Fragments arranged by size: 1842 1784 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3569 1391 Size: 2178 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2253 928 Size: 971 354 4925 Fragments arranged by size: 4925 971 354 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4441 4475 1903 Size: 34 2504 34 3678 Fragments arranged by size: 3678 2504 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4142 5352 4142 Size: 1210 5040 BclI T'GATC_A Cuts at: 1548 1548 Size: 6250 BfrBI ATG'CAT Cuts at: 2291 2964 2291 Size: 673 5577 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4861 136 Size: 107 122 71 631 605 3189 1525 Fragments arranged by size: 3189 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2073 3538 6245 2073 Size: 1465 2707 2078 Fragments arranged by size: 2707 2078 1465 BlpI GC'TnA_GC Cuts at: 3667 3667 Size: 6250 Bme1580I G_kGCm'C Cuts at: 1790 3356 4311 5467 1790 Size: 1566 955 1156 2573 Fragments arranged by size: 2573 1566 1156 955 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6250 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2245 3981 4939 Size: 416 53 83 399 24 828 1736 958 Cuts at: 4939 442 Size: 1753 Fragments arranged by size: 1753 1736 958 828 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6250 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2057 2378 2441 4910 1239 Size: 818 321 63 2469 2579 Fragments arranged by size: 2579 2469 818 321 63 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2333 2596 3317 4375 5240 5538 2333 Size: 263 721 1058 865 298 3045 Fragments arranged by size: 3045 1058 865 721 298 263 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4913 882 Size: 617 3414 2219 Fragments arranged by size: 3414 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3493 4041 493 Size: 711 2289 548 2702 Fragments arranged by size: 2702 2289 711 548 BsaBI GATnn'nnATC Cuts at: 2856 6244 2856 Size: 3388 2862 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4057 4439 5074 275 Size: 53 83 186 936 2524 382 635 1451 Fragments arranged by size: 2524 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4683 5081 5355 5502 5913 4683 Size: 398 274 147 411 5020 Fragments arranged by size: 5020 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2039 2069 2354 2384 3355 Size: 30 165 30 636 30 285 30 971 Cuts at: 3355 3385 1178 Size: 30 4043 Fragments arranged by size: 4043 971 636 285 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2375 2407 1186 Size: 1189 32 5029 Fragments arranged by size: 5029 1189 32 BseYI C'CCAG_C Cuts at: 5453 5949 5453 Size: 496 5754 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5677 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3700 4461 5489 1540 Size: 275 186 1699 761 1028 2301 Fragments arranged by size: 2301 1699 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2813 4311 4396 5467 2813 Size: 1498 85 1071 3596 Fragments arranged by size: 3596 1498 1071 85 BsmI GAATG_Cn' Cuts at: 2109 3761 3854 2109 Size: 1652 93 4505 Fragments arranged by size: 4505 1652 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2813 3356 3569 4311 4396 5467 1391 Size: 399 1023 543 213 742 85 1071 2174 Fragments arranged by size: 2174 1071 1023 742 543 399 213 85 BspEI T'CCGG_A Cuts at: 5913 5913 Size: 6250 BspHI T'CATG_A Cuts at: 2517 4139 5869 2517 Size: 1622 1730 2898 Fragments arranged by size: 2898 1730 1622 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2236 3605 844 Size: 1392 1369 3489 Fragments arranged by size: 3489 1392 1369 BsrBI CCG'CTC Cuts at: 1394 1823 3168 4137 1394 Size: 429 1345 969 3507 Fragments arranged by size: 3507 1345 969 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4745 4919 65 Size: 1183 3497 174 1396 Fragments arranged by size: 3497 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4894 5081 1700 Size: 110 3084 187 2869 Fragments arranged by size: 3084 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6250 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6250 BssSI C'ACGA_G Cuts at: 4310 5322 4310 Size: 1012 5238 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6250 BstBI TT'CG_AA Cuts at: 2101 5054 2101 Size: 2953 3297 BstEII G'GTnAC_C Cuts at: 1184 3095 3161 3312 3326 1184 Size: 1911 66 151 14 4108 Fragments arranged by size: 4108 1911 151 66 14 BstYI r'GATC_y Cuts at: 1972 2073 3538 3580 3947 4332 4349 5801 5887 Size: 101 1465 42 367 385 17 1452 86 Cuts at: 5887 5899 6245 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1465 1452 385 367 346 101 86 42 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2572 3260 513 Size: 552 939 568 688 3503 Fragments arranged by size: 3503 939 688 568 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5511 Fragments arranged by size: 5511 683 56 BtsI GCAGTG_nn' Cuts at: 2883 2895 3411 3766 4560 4580 2883 Size: 12 516 355 794 20 4553 Fragments arranged by size: 4553 794 516 355 20 12 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6250 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2047 2082 529 Size: 35 1483 35 4697 Fragments arranged by size: 4697 1483 35 35 DraI TTT'AAA Cuts at: 2710 2904 3554 2710 Size: 194 650 5406 Fragments arranged by size: 5406 650 194 DraIII CAC_nnn'GTG Cuts at: 3496 3496 Size: 6250 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2606 5257 809 Size: 507 1290 2651 1802 Fragments arranged by size: 2651 1802 1290 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2575 3697 4586 Size: 54 1425 50 275 18 745 1122 889 Cuts at: 4586 6215 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1122 889 745 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3697 1537 Size: 275 1885 4090 Fragments arranged by size: 4090 1885 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2313 4180 1353 Size: 960 1867 3423 Fragments arranged by size: 3423 1867 960 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4837 5211 5357 387 Size: 1064 138 3248 374 146 1280 Fragments arranged by size: 3248 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2057 2148 2378 2441 2849 3144 3527 Size: 393 425 91 230 63 408 295 383 Cuts at: 3527 4313 4910 5697 5965 1239 Size: 786 597 787 268 1524 Fragments arranged by size: 1524 787 786 597 425 408 393 383 295 268 230 91 63 EcoNI CCTnn'n_nnAGG Cuts at: 3321 3378 3321 Size: 57 6193 EcoRI G'AATT_C Cuts at: 2765 3559 3937 2765 Size: 794 378 5078 Fragments arranged by size: 5078 794 378 FspI TGC'GCA Cuts at: 4756 4756 Size: 6250 HaeII r_GCGC'y Cuts at: 1678 5397 1678 Size: 3719 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2135 2167 2323 2355 3377 Size: 32 135 32 563 32 156 32 1022 Cuts at: 3377 3409 4891 4923 4965 4997 1373 Size: 32 1482 32 42 32 2626 Fragments arranged by size: 2626 1482 1022 563 156 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3594 3840 669 Size: 988 281 1656 246 3079 Fragments arranged by size: 3079 1656 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6250 HpaI GTT'AAC Cuts at: 3840 3840 Size: 6250 KpnI G_GTAC'C Cuts at: 3575 3575 Size: 6250 MfeI C'AATT_G Cuts at: 3849 3849 Size: 6250 MluI A'CGCG_T Cuts at: 6126 6126 Size: 6250 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2491 5364 5548 1305 Size: 1186 2873 184 2007 Fragments arranged by size: 2873 2007 1186 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2577 10 Size: 54 1425 343 745 3683 Fragments arranged by size: 3683 1425 745 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2474 2888 4208 4567 4726 518 Size: 648 1308 414 1320 359 159 2042 Fragments arranged by size: 2042 1320 1308 648 414 359 159 MspA1I CmG'CkG Cuts at: 1606 2126 2419 3262 3666 4343 5491 5736 1606 Size: 520 293 843 404 677 1148 245 2120 Fragments arranged by size: 2120 1148 843 677 520 404 293 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2572 513 Size: 552 939 568 4191 Fragments arranged by size: 4191 939 568 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6250 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6250 NotI GC'GGCC_GC Cuts at: 3697 3697 Size: 6250 NsiI A_TGCA'T Cuts at: 2293 2966 2293 Size: 673 5577 NspI r_CATG'y Cuts at: 1132 1229 3608 5153 1132 Size: 97 2379 1545 2229 Fragments arranged by size: 2379 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6250 PciI A'CATG_T Cuts at: 5149 5149 Size: 6250 PflMI CCAn_nnn'nTGG Cuts at: 1108 3106 1108 Size: 1998 4252 PmeI GTTT'AAAC Cuts at: 3554 3554 Size: 6250 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3137 3169 3355 3387 4319 4351 5832 5864 3137 Size: 32 186 32 932 32 1481 32 3523 Fragments arranged by size: 3523 1481 932 186 32 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6250 PsiI TTA'TAA Cuts at: 2856 3820 2856 Size: 964 5286 PstI C_TGCA'G Cuts at: 830 2189 2247 2890 3602 830 Size: 1359 58 643 712 3478 Fragments arranged by size: 3478 1359 712 643 58 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6250 PvuII CAG'CTG Cuts at: 2126 2419 2126 Size: 293 5957 SacII CC_GC'GG Cuts at: 3263 3263 Size: 6250 SalI G'TCGA_C Cuts at: 1936 3592 1936 Size: 1656 4594 SbfI CC_TGCA'GG Cuts at: 3602 3602 Size: 6250 ScaI AGT'ACT Cuts at: 3016 4498 6210 3016 Size: 1482 1712 3056 Fragments arranged by size: 3056 1712 1482 SfcI C'TryA_G Cuts at: 826 1045 2185 2243 2655 2886 3186 3291 3598 Size: 219 1140 58 412 231 300 105 307 Cuts at: 3598 4733 5414 5605 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1140 1135 681 412 307 300 231 219 191 105 58 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6250 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6250 SmaI CCC'GGG Cuts at: 1872 3577 1872 Size: 1705 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2312 2611 3332 4354 5255 5517 Size: 197 926 369 299 721 1022 901 262 Cuts at: 5517 820 Size: 1553 Fragments arranged by size: 1553 1022 926 901 721 369 299 262 197 SnaBI TAC'GTA Cuts at: 493 4041 493 Size: 3548 2702 SpeI A'CTAG_T Cuts at: 152 3017 152 Size: 2865 3385 SphI G_CATG'C Cuts at: 1132 3608 1132 Size: 2476 3774 SspI AAT'ATT Cuts at: 5 52 2634 3075 4174 5 Size: 47 2582 441 1099 2081 Fragments arranged by size: 2582 2081 1099 441 47 StuI AGG'CCT Cuts at: 1517 2210 1517 Size: 693 5557 StyI C'CwwG_G Cuts at: 513 1065 2004 2023 2381 2572 2701 3689 513 Size: 552 939 19 358 191 129 988 3074 Fragments arranged by size: 3074 988 939 552 358 191 129 19 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2105 4277 4294 4447 4632 5077 325 Size: 1255 525 2172 17 153 185 445 1498 Fragments arranged by size: 2172 1498 1255 525 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2986 3014 4496 Size: 274 80 33 51 1443 1009 28 1482 Cuts at: 4496 6208 96 Size: 1712 138 Fragments arranged by size: 1712 1482 1443 1009 274 138 80 51 33 28 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2039 2071 3482 3514 6210 6242 2039 Size: 32 1411 32 2696 32 2047 Fragments arranged by size: 2696 2047 1411 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6250 XbaI T'CTAG_A Cuts at: 3155 3586 3155 Size: 431 5819 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6250 XmaI C'CCGG_G Cuts at: 1870 3575 1870 Size: 1705 4545 XmnI GAAnn'nnTTC Cuts at: 4379 4379 Size: 6250 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4058 276 Size: 53 83 186 936 2524 2468 Fragments arranged by size: 2524 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI