CERS4 ORF clone pFN21AB8142

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Halotag ORF Clone pFN21AB8142

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Clone info.

Species

human

Clone Name

pFN21AB8142 Sequence (with vector) Restriction Map

Product ID

FHC02612

Flexi Clone PID

FXC02612

Original Clone PID

ORK02612

ORF Sequence

DNA sequence (1182 bp)
Protein sequence (394 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB463826]

Gene

KIBB8142

Symbol

CERS4

Alias : LASS4, Trh1

Description

ceramide synthase 4

Original Clone ID

cp00502

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1182 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 394 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS12197.1 6.1e-156 99.2 CERS4 CCDS973.1 2.7e-71 49.6 CERS2 CCDS10384.1 1.9e-64 46.5 CERS3 CCDS8801.1 1.8e-60 43.9 CERS5 CCDS2228.1 7.4e-59 43.1 CERS6 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR001356 82 126 PF00046 Homeobox IPR006634 132 325 PF03798 TRAM/LAG1/CLN8 homology domain HMMSmart IPR006634 131 332 SM00724 TRAM/LAG1/CLN8 homology domain ProfileScan IPR001356 84 128 PS50071 Homeobox IPR006634 131 332 PS50922 TRAM/LAG1/CLN8 homology domain
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 134 CEASWRFLFYLSSFVGGLSVLY 155 SECONDARY 22 2 179 LYWWYLLELGFYLSLLIRLPFDV 201 SECONDARY 23 3 206 FKEQVIHHFVAVILMTFSYSANL 228 SECONDARY 23 4 261 CDALFLIFSFVFFYTRLVLFPTQ 283 PRIMARY 23 5 308 LLMLLQLLHVFWSCLILRMLYSF 330 PRIMARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) XP_011526595 394 615334 99.2 100.0 XP_011526592 394 615334 99.2 100.0 XP_011526596 394 615334 99.2 100.0 XP_011526594 394 615334 99.2 100.0 NP_078828 394 615334 99.2 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB8142 Download

>KIBB8142  1182 bp
ATGCTGTCCAGTTTCAACGAGTGGTTTTGGCAGGACAGGTTCTGGTTACCACCCAATGTC
ACGTGGACAGAGCTAGAAGACCGGGATGGCCGTGTCTACCCCCACCCCCAGGACTTGTTG
GCAGCCCTGCCCCTGGCGCTGGTCCTCCTGGCCATGCGCCTTGCCTTTGAGAGATTCATT
GGCCTGCCCCTGAGCCGGTGGCTGGGTGTGAGGGATCAGACCAGGAGGCAAGTGAAGCCC
AACGCCACGCTGGAGAAACACTTCCTCACGGAAGGGCACAGGCCCAAGGAGCCCCAGCTG
TCTCTCCTGGCCGCCCAGTGTGGCCTCACGCTGCAGCAGACCCAGCGATGGTTCCGGAGA
CGCCGGAACCAGGATCGACCCCAGCTGACCAAGAAGTTCTGTGAGGCCAGCTGGAGGTTT
CTCTTCTACCTGTCCTCCTTCGTGGGCGGCCTCTCGGTCCTGTACCACGAGTCATGGCTG
TGGGCACCAGTAATGTGCTGGGACAGGTACCCAAACCAGACTCTGAAGCCATCCCTGTAC
TGGTGGTACCTCTTGGAGCTGGGTTTCTACCTCTCACTGCTAATCAGGCTGCCCTTTGAT
GTCAAGCGCAAGGATTTCAAGGAGCAGGTGATACACCACTTCGTGGCGGTCATCCTGATG
ACCTTCTCCTACAGTGCCAACCTGCTGCGCATTGGCTCTCTGGTGCTGCTGTTACATGAT
TCCTCTGACTACCTGCTGGAGGCCTGTAAGATGGTCAACTACATGCAGTATCAGCAAGTG
TGCGACGCTCTCTTCCTCATCTTCTCCTTTGTCTTCTTCTACACCCGACTGGTCCTCTTT
CCCACCCAGATCCTCTACACCATATACTACGAGTCCATCAGCAACAGGGGCCCCTTCTTC
GGCTACTACTTCTTCAACGGGCTTCTGATGTTGCTGCAGCTGCTGCACGTGTTCTGGTCT
TGCCTCATTCTGCGCATGCTCTATAGCTTCATGAAGAAGGGCCAGATGGAGAAGGACATT
CGTAGTGATGTAGAAGAATCAGACTCCAGTGAGGAGGTGGCGGCGGCCCAGGAACCTCTG
CAGCTAAAGAACGGGGCAGCTGGAGGGCCCAGGCCAGCCCCCACTGATGGCCCTCAGAGC
CGGGTGGCCGGGCGTCTGACCAACAGGCACACAACAGCCACA

ORF Protein

Cloned ORF protein sequence for pFN21AB8142 Download

>KIBB8142  394 aa
MLSSFNEWFWQDRFWLPPNVTWTELEDRDGRVYPHPQDLLAALPLALVLLAMRLAFERFI
GLPLSRWLGVRDQTRRQVKPNATLEKHFLTEGHRPKEPQLSLLAAQCGLTLQQTQRWFRR
RRNQDRPQLTKKFCEASWRFLFYLSSFVGGLSVLYHESWLWAPVMCWDRYPNQTLKPSLY
WWYLLELGFYLSLLIRLPFDVKRKDFKEQVIHHFVAVILMTFSYSANLLRIGSLVLLLHD
SSDYLLEACKMVNYMQYQQVCDALFLIFSFVFFYTRLVLFPTQILYTIYYESISNRGPFF
GYYFFNGLLMLLQLLHVFWSCLILRMLYSFMKKGQMEKDIRSDVEESDSSEEVAAAQEPL
QLKNGAAGGPRPAPTDGPQSRVAGRLTNRHTTAT

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB8142 Download

>pFN21AB8142  5887 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGCTGTCCAGTTTCAACGAGTGGTTTTGGCAGGA
CAGGTTCTGGTTACCACCCAATGTCACGTGGACAGAGCTAGAAGACCGGGATGGCCGTGT
CTACCCCCACCCCCAGGACTTGTTGGCAGCCCTGCCCCTGGCGCTGGTCCTCCTGGCCAT
GCGCCTTGCCTTTGAGAGATTCATTGGCCTGCCCCTGAGCCGGTGGCTGGGTGTGAGGGA
TCAGACCAGGAGGCAAGTGAAGCCCAACGCCACGCTGGAGAAACACTTCCTCACGGAAGG
GCACAGGCCCAAGGAGCCCCAGCTGTCTCTCCTGGCCGCCCAGTGTGGCCTCACGCTGCA
GCAGACCCAGCGATGGTTCCGGAGACGCCGGAACCAGGATCGACCCCAGCTGACCAAGAA
GTTCTGTGAGGCCAGCTGGAGGTTTCTCTTCTACCTGTCCTCCTTCGTGGGCGGCCTCTC
GGTCCTGTACCACGAGTCATGGCTGTGGGCACCAGTAATGTGCTGGGACAGGTACCCAAA
CCAGACTCTGAAGCCATCCCTGTACTGGTGGTACCTCTTGGAGCTGGGTTTCTACCTCTC
ACTGCTAATCAGGCTGCCCTTTGATGTCAAGCGCAAGGATTTCAAGGAGCAGGTGATACA
CCACTTCGTGGCGGTCATCCTGATGACCTTCTCCTACAGTGCCAACCTGCTGCGCATTGG
CTCTCTGGTGCTGCTGTTACATGATTCCTCTGACTACCTGCTGGAGGCCTGTAAGATGGT
CAACTACATGCAGTATCAGCAAGTGTGCGACGCTCTCTTCCTCATCTTCTCCTTTGTCTT
CTTCTACACCCGACTGGTCCTCTTTCCCACCCAGATCCTCTACACCATATACTACGAGTC
CATCAGCAACAGGGGCCCCTTCTTCGGCTACTACTTCTTCAACGGGCTTCTGATGTTGCT
GCAGCTGCTGCACGTGTTCTGGTCTTGCCTCATTCTGCGCATGCTCTATAGCTTCATGAA
GAAGGGCCAGATGGAGAAGGACATTCGTAGTGATGTAGAAGAATCAGACTCCAGTGAGGA
GGTGGCGGCGGCCCAGGAACCTCTGCAGCTAAAGAACGGGGCAGCTGGAGGGCCCAGGCC
AGCCCCCACTGATGGCCCTCAGAGCCGGGTGGCCGGGCGTCTGACCAACAGGCACACAAC
AGCCACAGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAG
GCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCA
CCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGA
TACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGT
GAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAAC
AACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTA
AGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTT
GCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTC
TGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAAT
GTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATG
AGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAA
CATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCAC
CCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTAC
ATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTT
CCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCC
GGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCA
CCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCC
ATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAG
GAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAA
CCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATG
GCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAA
TTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCG
GCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATT
GCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGT
CAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAG
CATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGG
CGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAG
GCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCC
GCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAG
GACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGA
CCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTC
ATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTG
TGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGT
CCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCA
GAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACA
CTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAG
TTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCA
AGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGG
GGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAA
AAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGC
CCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAA
TGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGAT
TGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCT
GAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCC
CTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATC
GAGATCT