(Circular) (Six-base) MAPSORT of: pFN21AB8142.seq Check: 2348 from: 1 to: 5887 pFN21AB8142 5887 bp With 182 enzymes: SgfI * February 27, 2009 14:40 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2620 2620 Size: 5887 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3697 278 Size: 53 83 186 936 2161 2468 Fragments arranged by size: 2468 2161 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2100 3230 1937 Size: 163 1130 4594 Fragments arranged by size: 4594 1130 163 Acc65I G'GTAC_C Cuts at: 2511 2550 3208 2511 Size: 39 658 5190 Fragments arranged by size: 5190 658 39 AclI AA'CG_TT Cuts at: 1563 4014 4387 1563 Size: 2451 373 3063 Fragments arranged by size: 3063 2451 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2549 3950 5334 5602 1632 Size: 917 1401 1384 268 1917 Fragments arranged by size: 1917 1401 1384 917 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5690 AflIII A'CryG_T Cuts at: 1203 2952 4786 5763 1203 Size: 1749 1834 977 1327 Fragments arranged by size: 1834 1749 1327 977 AgeI A'CCGG_T Cuts at: 4718 4718 Size: 5887 AhdI GACnn_n'nnGTC Cuts at: 4616 4616 Size: 5887 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5887 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5887 AlwNI CAG_nnn'CTG Cuts at: 2046 2527 2947 3630 5202 2046 Size: 481 420 683 1572 2731 Fragments arranged by size: 2731 1572 683 481 420 ApaI G_GGCC'C Cuts at: 2897 3114 2897 Size: 217 5670 ApaLI G'TGCA_C Cuts at: 3944 5100 3944 Size: 1156 4731 ApoI r'AATT_y Cuts at: 1493 3196 3422 3574 5683 1493 Size: 1703 226 152 2109 1697 Fragments arranged by size: 2109 1703 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4441 160 Size: 872 3409 1606 Fragments arranged by size: 3409 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5887 AvaI C'yCGr_G Cuts at: 1870 1943 3212 1870 Size: 73 1269 4545 Fragments arranged by size: 4545 1269 73 BamHI G'GATC_C Cuts at: 3217 3584 3217 Size: 367 5520 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2488 2511 2550 3208 4663 Size: 325 217 627 701 23 39 658 1455 Cuts at: 4663 618 Size: 1842 Fragments arranged by size: 1842 1455 701 658 627 325 217 39 23 BanII G_rGCy'C Cuts at: 1391 2298 2897 3114 3206 1391 Size: 907 599 217 92 4072 Fragments arranged by size: 4072 907 599 217 92 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2088 2809 928 Size: 971 189 721 4006 Fragments arranged by size: 4006 971 721 189 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4078 4112 1903 Size: 34 50 34 2057 34 3678 Fragments arranged by size: 3678 2057 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3779 4989 3779 Size: 1210 4677 BclI T'GATC_A Cuts at: 1548 1548 Size: 5887 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4498 136 Size: 107 122 71 631 605 2826 1525 Fragments arranged by size: 2826 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5882 5882 Size: 5887 BlpI GC'TnA_GC Cuts at: 3304 3304 Size: 5887 Bme1580I G_kGCm'C Cuts at: 1790 2283 2491 2897 3114 3948 5104 1790 Size: 493 208 406 217 834 1156 2573 Fragments arranged by size: 2573 1156 834 493 406 217 208 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5887 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2314 3618 4576 Size: 416 53 83 399 24 897 1304 958 Cuts at: 4576 442 Size: 1753 Fragments arranged by size: 1753 1304 958 897 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5887 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2276 2437 2762 3034 3126 4547 1239 Size: 1037 161 325 272 92 1421 2579 Fragments arranged by size: 2579 1421 1037 325 272 161 92 Bpu10I CC'TnA_GC Cuts at: 2195 2195 Size: 5887 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4012 4877 5175 4012 Size: 865 298 4724 Fragments arranged by size: 4724 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4550 882 Size: 617 3051 2219 Fragments arranged by size: 3051 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2067 2953 3678 493 Size: 711 863 886 725 2702 Fragments arranged by size: 2702 886 863 725 711 BsaBI GATnn'nnATC Cuts at: 5881 5881 Size: 5887 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2365 3157 3694 4076 Size: 53 83 186 936 832 792 537 382 Cuts at: 4076 4711 275 Size: 635 1451 Fragments arranged by size: 1451 936 832 792 635 537 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2358 4320 4718 4992 5139 5550 2358 Size: 1962 398 274 147 411 2695 Fragments arranged by size: 2695 1962 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2423 2453 1178 Size: 30 165 30 1020 30 4612 Fragments arranged by size: 4612 1020 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3071 1186 Size: 1885 4002 BseYI C'CCAG_C Cuts at: 2206 2298 2346 2385 2502 2563 5090 5586 2206 Size: 92 48 39 117 61 2527 496 2507 Fragments arranged by size: 2527 2507 496 117 92 61 48 39 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2933 1163 Size: 573 1197 4117 Fragments arranged by size: 4117 1197 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3337 4098 5126 1540 Size: 275 186 1336 761 1028 2301 Fragments arranged by size: 2301 1336 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3948 4033 5104 3948 Size: 85 1071 4731 Fragments arranged by size: 4731 1071 85 BsmI GAATG_Cn' Cuts at: 3398 3491 3398 Size: 93 5794 BsmBI CGTCTCn'nnnn_ Cuts at: 2356 2356 Size: 5887 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2283 2298 2491 2897 3114 3206 3948 Size: 399 493 15 193 406 217 92 742 Cuts at: 3948 4033 5104 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 493 406 399 217 193 92 85 15 BspEI T'CCGG_A Cuts at: 2358 5550 2358 Size: 3192 2695 BspHI T'CATG_A Cuts at: 2994 3776 5506 2994 Size: 782 1730 3375 Fragments arranged by size: 3375 1730 782 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2620 2694 2745 3242 844 Size: 1776 74 51 497 3489 Fragments arranged by size: 3489 1776 497 74 51 BsrBI CCG'CTC Cuts at: 1394 1823 3774 1394 Size: 429 1951 3507 Fragments arranged by size: 3507 1951 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4382 4556 65 Size: 1183 3134 174 1396 Fragments arranged by size: 3134 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2199 4531 4718 1700 Size: 110 389 2332 187 2869 Fragments arranged by size: 2869 2332 389 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5887 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5887 BssSI C'ACGA_G Cuts at: 2471 3947 4959 2471 Size: 1476 1012 3399 Fragments arranged by size: 3399 1476 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2947 794 Size: 2153 3734 BstBI TT'CG_AA Cuts at: 4691 4691 Size: 5887 BstEII G'GTnAC_C Cuts at: 1184 2049 1184 Size: 865 5022 BstYI r'GATC_y Cuts at: 1972 2853 3217 3584 3969 3986 5438 5524 5536 Size: 881 364 367 385 17 1452 86 12 Cuts at: 5536 5882 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 881 385 367 364 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5335 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2365 487 Size: 683 56 1139 4009 Fragments arranged by size: 4009 1139 683 56 BtsI GCAGTG_nn' Cuts at: 2579 3403 4197 4217 2579 Size: 824 794 20 4249 Fragments arranged by size: 4249 824 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5887 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5852 DraI TTT'AAA Cuts at: 3191 3191 Size: 5887 DraIII CAC_nnn'GTG Cuts at: 2647 2647 Size: 5887 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4894 809 Size: 507 3578 1802 Fragments arranged by size: 3578 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2092 2154 2313 Size: 54 1425 50 275 18 262 62 159 Cuts at: 2313 3150 3334 4223 5852 8 Size: 837 184 889 1629 43 Fragments arranged by size: 1629 1425 889 837 275 262 184 159 62 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3334 1537 Size: 275 1522 4090 Fragments arranged by size: 4090 1522 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2432 2801 3817 1353 Size: 1079 369 1016 3423 Fragments arranged by size: 3423 1079 1016 369 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4474 4848 4994 387 Size: 1064 138 2885 374 146 1280 Fragments arranged by size: 2885 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2276 2437 2549 2762 3034 3126 3950 Size: 393 644 161 112 213 272 92 824 Cuts at: 3950 4547 5334 5602 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 824 787 644 597 393 272 268 213 161 112 92 EcoNI CCTnn'n_nnAGG Cuts at: 2741 2741 Size: 5887 EcoO109I rG'GnC_Cy Cuts at: 2893 2894 3110 2893 Size: 1 216 5670 Fragments arranged by size: 5670 216 1 EcoRI G'AATT_C Cuts at: 3196 3574 3196 Size: 378 5509 FspI TGC'GCA Cuts at: 2693 2978 4393 2693 Size: 285 1415 4187 Fragments arranged by size: 4187 1415 285 HaeII r_GCGC'y Cuts at: 1678 2144 5034 1678 Size: 466 2890 2531 Fragments arranged by size: 2890 2531 466 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2823 2855 4528 4560 4602 Size: 32 135 32 1251 32 1673 32 42 Cuts at: 4602 4634 1373 Size: 32 2626 Fragments arranged by size: 2626 1673 1251 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2761 3231 3477 669 Size: 988 281 823 470 246 3079 Fragments arranged by size: 3079 988 823 470 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5887 HpaI GTT'AAC Cuts at: 3477 3477 Size: 5887 KpnI G_GTAC'C Cuts at: 2515 2554 3212 2515 Size: 39 658 5190 Fragments arranged by size: 5190 658 39 MfeI C'AATT_G Cuts at: 3486 3486 Size: 5887 MluI A'CGCG_T Cuts at: 5763 5763 Size: 5887 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5001 5185 1305 Size: 3696 184 2007 Fragments arranged by size: 3696 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2156 10 Size: 54 1425 343 324 3741 Fragments arranged by size: 3741 1425 343 324 54 MslI CAynn'nnrTG Cuts at: 518 1166 2482 2660 3845 4204 4363 518 Size: 648 1316 178 1185 359 159 2042 Fragments arranged by size: 2042 1316 1185 648 359 178 159 MspA1I CmG'CkG Cuts at: 1606 2302 2389 2415 2944 3104 3303 3980 5128 Size: 696 87 26 529 160 199 677 1148 Cuts at: 5128 5373 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 696 677 529 245 199 160 87 26 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5335 NdeI CA'TA_TG Cuts at: 387 387 Size: 5887 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5887 NotI GC'GGCC_GC Cuts at: 3334 3334 Size: 5887 NspI r_CATG'y Cuts at: 1132 1229 2770 2983 3245 4790 1132 Size: 97 1541 213 262 1545 2229 Fragments arranged by size: 2229 1545 1541 262 213 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5887 PciI A'CATG_T Cuts at: 4786 4786 Size: 5887 PflMI CCAn_nnn'nTGG Cuts at: 1108 2647 1108 Size: 1539 4348 PmeI GTTT'AAAC Cuts at: 3191 3191 Size: 5887 PmlI CAC'GTG Cuts at: 2067 2953 2067 Size: 886 5001 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3956 3988 5469 5501 3956 Size: 32 1481 32 4342 Fragments arranged by size: 4342 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5887 PsiI TTA'TAA Cuts at: 3457 3457 Size: 5887 PspOMI G'GGCC_C Cuts at: 2893 3110 2893 Size: 217 5670 PstI C_TGCA'G Cuts at: 830 2340 2943 3087 3239 830 Size: 1510 603 144 152 3478 Fragments arranged by size: 3478 1510 603 152 144 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5887 PvuII CAG'CTG Cuts at: 2302 2389 2415 2944 3104 2302 Size: 87 26 529 160 5085 Fragments arranged by size: 5085 529 160 87 26 SalI G'TCGA_C Cuts at: 1936 3229 1936 Size: 1293 4594 SbfI CC_TGCA'GG Cuts at: 3239 3239 Size: 5887 ScaI AGT'ACT Cuts at: 4135 5847 4135 Size: 1712 4175 SfcI C'TryA_G Cuts at: 826 1045 2336 2674 2939 2986 3083 3235 4370 Size: 219 1291 338 265 47 97 152 1135 Cuts at: 4370 5051 5242 826 Size: 681 191 1471 Fragments arranged by size: 1471 1291 1135 681 338 265 219 191 152 97 47 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5887 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5887 SmaI CCC'GGG Cuts at: 1872 3214 1872 Size: 1342 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3991 4892 5154 820 Size: 197 926 2048 901 262 1553 Fragments arranged by size: 2048 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3678 493 Size: 3185 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5887 SphI G_CATG'C Cuts at: 1132 2983 3245 1132 Size: 1851 262 3774 Fragments arranged by size: 3774 1851 262 SspI AAT'ATT Cuts at: 5 52 3811 5 Size: 47 3759 2081 Fragments arranged by size: 3759 2081 47 StuI AGG'CCT Cuts at: 1517 2747 1517 Size: 1230 4657 StyI C'CwwG_G Cuts at: 513 1065 2289 3326 513 Size: 552 1224 1037 3074 Fragments arranged by size: 3074 1224 1037 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2071 2198 2449 2864 3914 3931 4084 Size: 1255 491 127 251 415 1050 17 153 Cuts at: 4084 4269 4714 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 1050 491 445 415 251 185 153 127 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2541 4133 5845 Size: 274 80 33 51 1443 564 1592 1712 Cuts at: 5845 96 Size: 138 Fragments arranged by size: 1712 1592 1443 564 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5847 5879 5847 Size: 32 5855 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5887 XbaI T'CTAG_A Cuts at: 3223 3223 Size: 5887 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2251 2499 2541 3058 2251 Size: 248 42 517 5080 Fragments arranged by size: 5080 517 248 42 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5887 XmaI C'CCGG_G Cuts at: 1870 3212 1870 Size: 1342 4545 XmnI GAAnn'nnTTC Cuts at: 2264 4016 2264 Size: 1752 4135 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3695 276 Size: 53 83 186 936 2161 2468 Fragments arranged by size: 2468 2161 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI