EIF3E ORF clone pFN21AB5352

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Halotag ORF Clone pFN21AB5352

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Clone info.

Species

human

Clone Name

pFN21AB5352 Sequence (with vector) Restriction Map

Product ID

FHC10395

Flexi Clone PID

FXC10395

Original Clone PID

ORK10395

ORF Sequence

DNA sequence (1335 bp)
Protein sequence (445 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB527268]

Gene

KIBB5352

Symbol

EIF3E

Alias : EIF3-P48, EIF3S6, INT6, eIF3-p46

Description

eukaryotic translation initiation factor 3, subunit E

Original Clone ID

bm04118

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1335 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 445 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS6308.1 2.5e-192 100.0 EIF3E The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR019010 5 138 PF09440 Eukaryotic translation initiation factor 3 (eIF3) IPR000717 292 394 PF01399 Proteasome component (PCI) domain HMMSmart IPR000717 327 411 SM00088 Proteasome component (PCI) domain
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_001559 445 602210 100.0 100.0 XP_016868887 480 602210 100.0 97.3 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB5352 Download

>KIBB5352  1335 bp
ATGGCGGAGTACGACTTGACTACTCGCATCGCGCACTTTTTGGATCGGCATCTAGTCTTT
CCGCTTCTTGAATTTCTCTCTGTAAAGGAGATATATAATGAAAAGGAATTATTACAAGGT
AAATTGGACCTTCTTAGTGATACCAACATGGTAGACTTTGCTATGGATGTATACAAAAAC
CTTTATTCTGATGATATTCCTCATGCTTTGAGAGAGAAAAGAACCACAGTGGTTGCACAA
CTGAAACAGCTTCAGGCAGAAACAGAACCAATTGTGAAGATGTTTGAAGATCCAGAAACT
ACAAGGCAAATGCAGTCAACCAGGGATGGTAGGATGCTCTTTGACTACCTGGCGGACAAG
CATGGTTTTAGGCAGGAATATTTAGATACACTCTACAGATATGCAAAATTCCAGTACGAA
TGTGGGAATTACTCAGGAGCAGCAGAATATCTTTATTTTTTTAGAGTGCTGGTTCCAGCA
ACAGATAGAAATGCTTTAAGTTCACTCTGGGGAAAGCTGGCCTCTGAAATCTTAATGCAG
AATTGGGATGCAGCCATGGAAGACCTTACACGGTTAAAAGAGACCATAGATAATAATTCT
GTGAGTTCTCCACTTCAGTCTCTTCAGCAGAGAACATGGCTCATTCACTGGTCTCTGTTT
GTTTTCTTCAATCACCCCAAAGGTCGCGATAATATTATTGACCTCTTCCTTTATCAGCCA
CAATATCTTAATGCAATTCAGACAATGTGTCCACACATTCTTCGCTATTTGACTACAGCA
GTCATAACAAACAAGGATGTTCGAAAACGTCGGCAGGTTCTAAAAGATCTAGTTAAAGTT
ATTCAACAGGAGTCTTACACATATAAAGACCCAATTACAGAATTTGTTGAATGTTTATAT
GTTAACTTTGACTTTGATGGGGCTCAGAAAAAGCTGAGGGAATGTGAATCAGTGCTTGTG
AATGACTTCTTCTTGGTGGCTTGTCTTGAGGATTTCATTGAAAATGCCCGTCTCTTCATA
TTTGAGACTTTCTGTCGCATCCACCAGTGTATCAGCATTAACATGTTGGCAGATAAATTG
AACATGACTCCAGAAGAAGCTGAAAGGTGGATTGTAAATTTGATTAGAAATGCAAGACTG
GATGCCAAGATTGATTCTAAATTAGGTCATGTGGTTATGGGTAACAATGCAGTCTCACCC
TATCAGCAAGTGATTGAAAAGACCAAAAGCCTTTCCTTTAGAAGCCAGATGTTGGCCATG
AATATTGAGAAGAAACTTAATCAGAATAGCAGGTCAGAGGCTCCTAACTGGGCAACTCAA
GATTCTGGCTTCTAC

ORF Protein

Cloned ORF protein sequence for pFN21AB5352 Download

>KIBB5352  445 aa
MAEYDLTTRIAHFLDRHLVFPLLEFLSVKEIYNEKELLQGKLDLLSDTNMVDFAMDVYKN
LYSDDIPHALREKRTTVVAQLKQLQAETEPIVKMFEDPETTRQMQSTRDGRMLFDYLADK
HGFRQEYLDTLYRYAKFQYECGNYSGAAEYLYFFRVLVPATDRNALSSLWGKLASEILMQ
NWDAAMEDLTRLKETIDNNSVSSPLQSLQQRTWLIHWSLFVFFNHPKGRDNIIDLFLYQP
QYLNAIQTMCPHILRYLTTAVITNKDVRKRRQVLKDLVKVIQQESYTYKDPITEFVECLY
VNFDFDGAQKKLRECESVLVNDFFLVACLEDFIENARLFIFETFCRIHQCISINMLADKL
NMTPEEAERWIVNLIRNARLDAKIDSKLGHVVMGNNAVSPYQQVIEKTKSLSFRSQMLAM
NIEKKLNQNSRSEAPNWATQDSGFY

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB5352 Download

>pFN21AB5352  6040 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCGGAGTACGACTTGACTACTCGCATCGCGCA
CTTTTTGGATCGGCATCTAGTCTTTCCGCTTCTTGAATTTCTCTCTGTAAAGGAGATATA
TAATGAAAAGGAATTATTACAAGGTAAATTGGACCTTCTTAGTGATACCAACATGGTAGA
CTTTGCTATGGATGTATACAAAAACCTTTATTCTGATGATATTCCTCATGCTTTGAGAGA
GAAAAGAACCACAGTGGTTGCACAACTGAAACAGCTTCAGGCAGAAACAGAACCAATTGT
GAAGATGTTTGAAGATCCAGAAACTACAAGGCAAATGCAGTCAACCAGGGATGGTAGGAT
GCTCTTTGACTACCTGGCGGACAAGCATGGTTTTAGGCAGGAATATTTAGATACACTCTA
CAGATATGCAAAATTCCAGTACGAATGTGGGAATTACTCAGGAGCAGCAGAATATCTTTA
TTTTTTTAGAGTGCTGGTTCCAGCAACAGATAGAAATGCTTTAAGTTCACTCTGGGGAAA
GCTGGCCTCTGAAATCTTAATGCAGAATTGGGATGCAGCCATGGAAGACCTTACACGGTT
AAAAGAGACCATAGATAATAATTCTGTGAGTTCTCCACTTCAGTCTCTTCAGCAGAGAAC
ATGGCTCATTCACTGGTCTCTGTTTGTTTTCTTCAATCACCCCAAAGGTCGCGATAATAT
TATTGACCTCTTCCTTTATCAGCCACAATATCTTAATGCAATTCAGACAATGTGTCCACA
CATTCTTCGCTATTTGACTACAGCAGTCATAACAAACAAGGATGTTCGAAAACGTCGGCA
GGTTCTAAAAGATCTAGTTAAAGTTATTCAACAGGAGTCTTACACATATAAAGACCCAAT
TACAGAATTTGTTGAATGTTTATATGTTAACTTTGACTTTGATGGGGCTCAGAAAAAGCT
GAGGGAATGTGAATCAGTGCTTGTGAATGACTTCTTCTTGGTGGCTTGTCTTGAGGATTT
CATTGAAAATGCCCGTCTCTTCATATTTGAGACTTTCTGTCGCATCCACCAGTGTATCAG
CATTAACATGTTGGCAGATAAATTGAACATGACTCCAGAAGAAGCTGAAAGGTGGATTGT
AAATTTGATTAGAAATGCAAGACTGGATGCCAAGATTGATTCTAAATTAGGTCATGTGGT
TATGGGTAACAATGCAGTCTCACCCTATCAGCAAGTGATTGAAAAGACCAAAAGCCTTTC
CTTTAGAAGCCAGATGTTGGCCATGAATATTGAGAAGAAACTTAATCAGAATAGCAGGTC
AGAGGCTCCTAACTGGGCAACTCAAGATTCTGGCTTCTACGTTTAAACGAATTCGGGCTC
GGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACA
AAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCC
TTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACA
ACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTT
GTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTT
CAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGT
AAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAG
TAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTT
TCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTT
TTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAA
TAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTT
TTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGAT
GCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAG
ATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTG
CTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATA
CACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGAT
GGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCC
AACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATG
GGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAAC
GACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACT
GGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAA
GTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCT
GGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCC
TCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGA
CAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAA
GCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAG
GGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAA
AGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATC
GACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCC
CTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCG
CCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTT
CGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACC
GCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGC
CACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAG
AGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCG
CTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAA
CCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAG
GATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACT
CACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAT
AGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAA
AAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAG
GTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTG
CCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATT
AAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGT
ACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT