(Circular) (Six-base) MAPSORT of: pFN21AB5352.seq Check: 8173 from: 1 to: 6040 pFN21AB5352 6040 bp With 182 enzymes: SgfI * October 20, 2009 10:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3850 278 Size: 53 83 186 936 2314 2468 Fragments arranged by size: 2468 2314 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2157 2175 3383 1937 Size: 220 18 1208 4594 Fragments arranged by size: 4594 1208 220 18 Acc65I G'GTAC_C Cuts at: 3361 3361 Size: 6040 AclI AA'CG_TT Cuts at: 1563 4167 4540 1563 Size: 2604 373 3063 Fragments arranged by size: 3063 2604 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2240 2603 2612 4103 5487 5755 1632 Size: 608 363 9 1491 1384 268 1917 Fragments arranged by size: 1917 1491 1384 608 363 268 9 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5843 AflIII A'CryG_T Cuts at: 1203 3066 4939 5916 1203 Size: 1863 1873 977 1327 Fragments arranged by size: 1873 1863 1327 977 AgeI A'CCGG_T Cuts at: 4871 4871 Size: 6040 AhdI GACnn_n'nnGTC Cuts at: 2751 3036 4769 2751 Size: 285 1733 4022 Fragments arranged by size: 4022 1733 285 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6040 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6040 AlwNI CAG_nnn'CTG Cuts at: 3783 5355 3783 Size: 1572 4468 ApaLI G'TGCA_C Cuts at: 4097 5253 4097 Size: 1156 4884 ApoI r'AATT_y Cuts at: 1493 2075 2411 2885 3121 3349 3575 3727 5836 Size: 582 336 474 236 228 226 152 2109 Cuts at: 5836 1493 Size: 1697 Fragments arranged by size: 2109 1697 582 474 336 236 228 226 152 AseI AT'TA_AT Cuts at: 160 1032 4594 160 Size: 872 3562 1606 Fragments arranged by size: 3562 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6040 AvaI C'yCGr_G Cuts at: 1870 1943 3365 1870 Size: 73 1422 4545 Fragments arranged by size: 4545 1422 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3037 3070 3037 Size: 33 6007 BamHI G'GATC_C Cuts at: 3370 3737 3370 Size: 367 5673 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3361 4816 618 Size: 325 217 627 1574 1455 1842 Fragments arranged by size: 1842 1574 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2929 3359 1391 Size: 1538 430 4072 Fragments arranged by size: 4072 1538 430 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2571 928 Size: 971 672 4397 Fragments arranged by size: 4397 971 672 BbvCI CC'TCA_GC Cuts at: 2939 2939 Size: 6040 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4231 4265 1903 Size: 34 2294 34 3678 Fragments arranged by size: 3678 2294 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3932 5142 3932 Size: 1210 4830 BclI T'GATC_A Cuts at: 1548 1548 Size: 6040 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4651 136 Size: 107 122 71 631 605 2979 1525 Fragments arranged by size: 2979 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2830 6035 2830 Size: 3205 2835 BlpI GC'TnA_GC Cuts at: 3457 3457 Size: 6040 Bme1580I G_kGCm'C Cuts at: 1790 4101 5257 1790 Size: 2311 1156 2573 Fragments arranged by size: 2573 2311 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6040 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3322 3771 4729 Size: 416 53 83 399 24 1905 449 958 Cuts at: 4729 442 Size: 1753 Fragments arranged by size: 1905 1753 958 449 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6040 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3078 4700 1239 Size: 1839 1622 2579 Fragments arranged by size: 2579 1839 1622 Bpu10I CC'TnA_GC Cuts at: 2939 2939 Size: 6040 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3011 3306 4165 5030 5328 3011 Size: 295 859 865 298 3723 Fragments arranged by size: 3723 865 859 298 295 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2579 2661 4703 882 Size: 617 1080 82 2042 2219 Fragments arranged by size: 2219 2042 1080 617 82 BsaAI yAC'GTr Cuts at: 493 1204 3831 493 Size: 711 2627 2702 Fragments arranged by size: 2702 2627 711 BsaBI GATnn'nnATC Cuts at: 2052 6034 2052 Size: 3982 2058 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3847 4229 4864 275 Size: 53 83 186 936 2314 382 635 1451 Fragments arranged by size: 2314 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4473 4871 5145 5292 5703 4473 Size: 398 274 147 411 4810 Fragments arranged by size: 4810 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5815 Fragments arranged by size: 5815 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6040 BseYI C'CCAG_C Cuts at: 5243 5739 5243 Size: 496 5544 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5467 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3490 4251 5279 1540 Size: 275 186 1489 761 1028 2301 Fragments arranged by size: 2301 1489 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4101 4186 5257 4101 Size: 85 1071 4884 Fragments arranged by size: 4884 1071 85 BsmI GAATG_Cn' Cuts at: 3551 3644 3551 Size: 93 5947 BsmBI CGTCTCn'nnnn_ Cuts at: 3020 3020 Size: 6040 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2929 3359 4101 4186 5257 1391 Size: 399 1139 430 742 85 1071 2174 Fragments arranged by size: 2174 1139 1071 742 430 399 85 BspEI T'CCGG_A Cuts at: 5703 5703 Size: 6040 BspHI T'CATG_A Cuts at: 3929 5659 3929 Size: 1730 4310 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2809 3285 3395 844 Size: 1965 476 110 3489 Fragments arranged by size: 3489 1965 476 110 BsrBI CCG'CTC Cuts at: 1394 1823 3927 1394 Size: 429 2104 3507 Fragments arranged by size: 3507 2104 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4535 4709 65 Size: 1183 3287 174 1396 Fragments arranged by size: 3287 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4684 4871 1700 Size: 110 2874 187 2869 Fragments arranged by size: 2874 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6040 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6040 BssSI C'ACGA_G Cuts at: 4100 5112 4100 Size: 1012 5028 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6040 BstBI TT'CG_AA Cuts at: 2806 4844 2806 Size: 2038 4002 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6040 BstYI r'GATC_y Cuts at: 1972 2293 2830 3370 3737 4122 4139 5591 5677 Size: 321 537 540 367 385 17 1452 86 Cuts at: 5677 5689 6035 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 540 537 385 367 346 321 86 17 12 BstZ17I GTA'TAC Cuts at: 2176 2176 Size: 6040 BtgI C'CryG_G Cuts at: 513 1065 2004 2559 513 Size: 552 939 555 3994 Fragments arranged by size: 3994 939 555 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2017 487 Size: 683 56 791 4510 Fragments arranged by size: 4510 791 683 56 BtsI GCAGTG_nn' Cuts at: 3556 4350 4370 3556 Size: 794 20 5226 Fragments arranged by size: 5226 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6040 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2218 2253 529 Size: 35 1654 35 4316 Fragments arranged by size: 4316 1654 35 35 DraI TTT'AAA Cuts at: 3344 3344 Size: 6040 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5047 809 Size: 507 3731 1802 Fragments arranged by size: 3731 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3258 3487 4376 Size: 54 1425 50 275 18 1428 229 889 Cuts at: 4376 6005 8 Size: 1629 43 Fragments arranged by size: 1629 1428 1425 889 275 229 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3487 1537 Size: 275 1675 4090 Fragments arranged by size: 4090 1675 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2631 2714 3023 3970 1353 Size: 1278 83 309 947 3423 Fragments arranged by size: 3423 1278 947 309 83 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2024 2372 4627 5001 5147 387 Size: 1064 138 435 348 2255 374 146 1280 Fragments arranged by size: 2255 1280 1064 435 374 348 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2240 2603 2612 3078 4103 4700 5487 Size: 393 608 363 9 466 1025 597 787 Cuts at: 5487 5755 1239 Size: 268 1524 Fragments arranged by size: 1524 1025 787 608 597 466 393 363 268 9 EcoRI G'AATT_C Cuts at: 3349 3727 3349 Size: 378 5662 FspI TGC'GCA Cuts at: 4546 4546 Size: 6040 HaeII r_GCGC'y Cuts at: 1678 5187 1678 Size: 3509 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2737 2769 3022 3054 4681 Size: 32 135 32 1165 32 253 32 1627 Cuts at: 4681 4713 4755 4787 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1627 1165 253 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2322 2908 3384 3630 669 Size: 988 281 384 586 476 246 3079 Fragments arranged by size: 3079 988 586 476 384 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6040 HpaI GTT'AAC Cuts at: 2908 3630 2908 Size: 722 5318 KpnI G_GTAC'C Cuts at: 3365 3365 Size: 6040 MfeI C'AATT_G Cuts at: 2274 3639 2274 Size: 1365 4675 MluI A'CGCG_T Cuts at: 5916 5916 Size: 6040 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5154 5338 1305 Size: 3849 184 2007 Fragments arranged by size: 3849 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3260 10 Size: 54 1425 343 1428 2790 Fragments arranged by size: 2790 1428 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3065 3998 4357 4516 518 Size: 648 1899 933 359 159 2042 Fragments arranged by size: 2042 1899 933 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3456 4133 5281 5526 1606 Size: 1850 677 1148 245 2120 Fragments arranged by size: 2120 1850 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2559 513 Size: 552 939 555 3994 Fragments arranged by size: 3994 939 555 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6040 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6040 NotI GC'GGCC_GC Cuts at: 3487 3487 Size: 6040 NruI TCG'CGA Cuts at: 2691 2691 Size: 6040 NspI r_CATG'y Cuts at: 1132 1229 3070 3398 4943 1132 Size: 97 1841 328 1545 2229 Fragments arranged by size: 2229 1841 1545 328 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6040 PciI A'CATG_T Cuts at: 3066 4939 3066 Size: 1873 4167 PflMI CCAn_nnn'nTGG Cuts at: 1108 3256 1108 Size: 2148 3892 PmeI GTTT'AAAC Cuts at: 3344 3344 Size: 6040 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2460 2492 4109 4141 5622 5654 2460 Size: 32 1617 32 1481 32 2846 Fragments arranged by size: 2846 1617 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6040 PsiI TTA'TAA Cuts at: 3610 3610 Size: 6040 PstI C_TGCA'G Cuts at: 830 3392 830 Size: 2562 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6040 SalI G'TCGA_C Cuts at: 1936 3382 1936 Size: 1446 4594 SbfI CC_TGCA'GG Cuts at: 3392 3392 Size: 6040 ScaI AGT'ACT Cuts at: 4288 6000 4288 Size: 1712 4328 SfcI C'TryA_G Cuts at: 826 1045 2398 2778 3388 4523 5204 5395 826 Size: 219 1353 380 610 1135 681 191 1471 Fragments arranged by size: 1471 1353 1135 681 610 380 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6040 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6040 SmaI CCC'GGG Cuts at: 1872 3367 1872 Size: 1495 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2990 3321 4144 5045 5307 820 Size: 197 926 1047 331 823 901 262 1553 Fragments arranged by size: 1553 1047 926 901 823 331 262 197 SnaBI TAC'GTA Cuts at: 493 3831 493 Size: 3338 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6040 SphI G_CATG'C Cuts at: 1132 3398 1132 Size: 2266 3774 SspI AAT'ATT Cuts at: 5 52 2384 2698 3268 3964 5 Size: 47 2332 314 570 696 2081 Fragments arranged by size: 2332 2081 696 570 314 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6040 StyI C'CwwG_G Cuts at: 513 1065 2004 2559 3479 513 Size: 552 939 555 920 3074 Fragments arranged by size: 3074 939 920 555 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4067 4084 4237 4422 4867 325 Size: 1255 2487 17 153 185 445 1498 Fragments arranged by size: 2487 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4286 5998 96 Size: 274 80 33 51 1443 2309 1712 138 Fragments arranged by size: 2309 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6000 6032 6000 Size: 32 6008 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6040 XbaI T'CTAG_A Cuts at: 3376 3376 Size: 6040 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2423 2423 Size: 6040 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6040 XmaI C'CCGG_G Cuts at: 1870 3365 1870 Size: 1495 4545 XmnI GAAnn'nnTTC Cuts at: 2969 4169 2969 Size: 1200 4840 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3848 276 Size: 53 83 186 936 2314 2468 Fragments arranged by size: 2468 2314 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NruI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BfrBI BplI BsiWI BstXI Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI