HECW1 ORF clone pFN21AA0322

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AA0322

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Clone info.

Species

human

Clone Name

pFN21AA0322 Sequence (with vector) Restriction Map

Product ID

FHC01066

Flexi Clone PID

FXC01066

ORF Sequence

DNA sequence (4818 bp)
Protein sequence (1606 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB384459]

Gene

KIAA0322

Symbol

HECW1

Alias : NEDL1

Description

HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1, transcript variant 1

Original Clone ID

hg00462s1

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 4818 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 1606 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS5469.2 0 99.9 HECW1 CCDS69286.1 8.3e-201 97.8 HECW1 CCDS77499.1 3.6e-144 60.7 HECW2 CCDS33354.1 4.3e-144 57.1 HECW2 CCDS45875.1 2.1e-51 34.5 NEDD4L The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR000008 212 301 PF00168 C2 calcium-dependent membrane targeting IPR001202 831 860 PF00397 WW/Rsp5/WWP IPR001202 1020 1049 PF00397 WW/Rsp5/WWP IPR000569 1302 1605 PF00632 HECT HMMSmart IPR000008 206 317 SM00239 C2 calcium-dependent membrane targeting IPR001202 830 862 SM00456 WW/Rsp5/WWP IPR001202 1019 1051 SM00456 WW/Rsp5/WWP IPR000569 1269 1606 SM00119 HECT ProfileScan IPR018029 206 302 PS50004 C2 membrane targeting protein IPR001202 829 862 PS50020 WW/Rsp5/WWP IPR001202 1018 1051 PS50020 WW/Rsp5/WWP IPR000569 1271 1606 PS50237 HECT
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_055867 1606 610384 99.9 100.0 XP_016867375 1606 610384 99.9 100.0 XP_011513522 1607 610384 99.9 100.0 XP_016867374 1607 610384 99.9 100.0 XP_005249722 1607 610384 99.9 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AA0322 Download

>KIAA0322  4818 bp
ATGCTGCTGCACCTGTGTAGTGTGAAGAATCTGTACCAGAACAGGTTTTTAGGCCTGGCC
GCCATGGCGTCTCCTTCTAGAAACTCCCAGAGCCGACGCCGGTGCAAGGAGCCGCTCCGA
TACAGCTACAACCCCGACCAGTTCCACAACATGGACCTCAGGGGCGGCCCCCACGATGGC
GTCACCATTCCCCGCTCCACCAGCGACACTGACCTGGTCACCTCGGACAGCCGCTCCACG
CTCATGGTCAGCAGCTCCTACTATTCCATCGGGCACTCTCAGGACCTGGTCATCCACTGG
GACATAAAGGAGGAAGTGGACGCTGGGGACTGGATTGGCATGTACCTCATTGATGAGGTC
TTGTCCGAAAACTTTCTGGACTATAAAAACCGTGGAGTCAATGGTTCTCATCGGGGCCAG
ATCATCTGGAAGATCGATGCCAGCTCGTACTTTGTGGAACCTGAAACTAAGATCTGCTTC
AAATACTACCATGGAGTGAGTGGGGCCCTGCGAGCAACCACCCCCAGTGTCACGGTCAAA
AACTCGGCAGCTCCTATTTTTAAAAGCATTGGTGCTGATGAGACCGTCCAAGGACAAGGA
AGTCGGAGGCTGATCAGCTTCTCTCTCTCAGATTTCCAAGCCATGGGGTTGAAGAAAGGG
ATGTTTTTCAACCCAGACCCTTATCTGAAGATTTCCATTCAGCCTGGGAAACACAGCATC
TTCCCCGCCCTCCCTCACCATGGACAGGAGAGGAGATCCAAGATCATAGGCAACACCGTG
AACCCCATCTGGCAGGCCGAGCAATTCAGTTTTGTGTCCTTGCCCACTGACGTGCTGGAA
ATTGAGGTGAAGGACAAGTTTGCCAAGAGCCGCCCCATCATCAAGCGCTTCTTGGGAAAG
CTGTCGATGCCCGTTCAAAGACTCCTGGAGAGACACGCCATAGGGGATAGGGTGGTCAGC
TACACACTTGGCCGCAGGCTTCCAACAGATCATGTGAGTGGACAGCTGCAATTCCGATTT
GAGATCACTTCCTCCATCCACCCAGATGATGAGGAGATTTCCCTGAGTACCGAGCCTGAG
TCAGCCCAAATTCAGGACAGCCCCATGAACAACCTGATGGAAAGCGGCAGTGGGGAACCT
CGGTCTGAGGCACCAGAGTCCTCTGAGAGCTGGAAGCCAGAGCAGCTGGGTGAGGGCAGT
GTCCCCGATCGTCCAGGGAACCAAAGCATAGAGCTTTCCAGACCAGCTGAGGAAGCAGCA
GTCATCACGGAGGCAGGAGACCAGGGCATGGTCTCTGTGGGACCTGAAGGGGCTGGGGAG
CTCCTGGCCCAGGTGCAAAAGGACATCCAGCCTGCCCCCAGTGCAGAAGAGCTGGCCGAG
CAGCTGGACCTGGGTGAGGAGGCATCAGCACTGCTGCTGGAAGACGGTGAAGCCCCAGCC
AGCACCAAGGAGGAGCCCTTGGAGGAGGAAGCAACGACCCAGAGCCGGGCTGGAAGGGAA
GAAGAGGAGAAGGAGCAGGAGGAGGAGGGAGATGTGTCCACCCTGGAGCAGGGAGAGGGC
AGGCTGCAGCTGCGGGCCTCGGTGAAGAGAAAAAGCAGGCCCTGCTCCTTGCCTGTGTCC
GAGCTGGAGACGGTGATCGCGTCAGCCTGCGGGGACCCCGAGACCCCGCGGACACACTAC
ATCCGCATCCACACCCTGCTGCACAGCATGCCCTCCGCCCAGGGCGGCAGCGCGGCAGAG
GAGGAGGACGGCGCGGAGGAGGAGTCCACCCTCAAGGACTCCTCGGAGAAGGATGGGCTC
AGCGAGGTGGACACGGTGGCCGCTGACCCGTCTGCCCTGGAAGAGGACAGAGAAGAGCCC
GAGGGGGCTACTCCAGGCACGGCGCACCCTGGCCACTCCGGGGGCCACTTCCCCAGCCTG
GCCAATGGCGCGGCCCAGGATGGCGACACGCACCCCAGCACCGGGAGCGAGAGCGACTCC
AGCCCCAGGCAAGGCGGGGACCACAGTTGCGAGGGCTGTGACGCGTCCTGCTGCAGCCCC
TCGTGCTACAGCTCCTCGTGCTACAGCACGTCCTGCTACAGCAGCTCGTGCTACAGCGCC
TCGTGCTACAGCCCCTCCTGCTACAACGGCAACAGGTTCGCCAGCCACACGCGCTTCTCC
TCCGTGGACAGCGCCAAGATCTCCGAGAGCACGGTCTTCTCCTCGCAAGACGACGAGGAG
GAGGAGAACAGCGCGTTCGAGTCGGTACCCGACTCCATGCAGAGCCCTGAGCTGGACCCG
GAGTCCACGAACGGCGCTGGGCCGTGGCAAGACGAGCTGGCCGCCCCTAGCGGGCACGTG
GAAAGAAGCCCGGAAGGTCTGGAATCCCCCGTGGCAGGTCCAAGCAATCGGAGAGAAGGT
GAATGTCCTATACTCCATAATTCCCAGCCAGTAAGCCAGCTTCCTTCCCTGAGGCCTGAA
CATCATCACTACCCAACAATCGATGAGCCTCTTCCACCAAACTGGGAAGCTCGAATTGAC
AGCCACGGGCGGGTCTTTTATGTGGACCACGTGAACCGCACAACCACCTGGCAGCGTCCG
ACGGCAGCAGCCACCCCGGATGGCATGCGGAGATCGGGGTCCATCCAGCAGATGGAGCAA
CTCAACAGGCGGTATCAAAACATTCAGCGAACCATTGCAACAGAGAGGTCCGAAGAAGAT
TCTGGCAGCCAAAGCTGCGAGCAAGCCCCAGCAGGAGGAGGCGGAGGTGGAGGGAGTGAC
TCAGAAGCCGAATCTTCCCAGTCCAGCTTAGATCTAAGGAGAGAGGGGTCACTTTCTCCA
GTGAACTCACAAAAAATCACCTTGCTGCTGCAGTCCCCAGCGGTCAAGTTCATCACCAAC
CCCGAGTTCTTCACTGTGCTACATGCCAATTATAGTGCCTACCGAGTCTTCACCAGTAGC
ACCTGCTTAAAGCACATGATTCTGAAAGTCCGACGGGATGCTCGCAATTTTGAACGCTAC
CAGCACAACCGGGACTTGGTGAATTTCATCAACATGTTCGCAGACACTCGGCTGGAACTG
CCCCGGGGCTGGGAGATCAAAACGGACCAGCAGGGAAAGTCTTTTTTCGTGGACCACAAC
AGTCGAGCTACCACTTTCATTGACCCCCGAATCCCTCTTCAGAACGGTCGTCTTCCCAAT
CATCTAACTCACCGACAGCACCTCCAGAGGCTCCGAAGTTACAGCGCTGGAGAGGCCTCA
GAAGTTTCTAGAAACAGAGGAGCCTCTTTACTGGCCAGGCCAGGACACAGCTTAGTAGCT
GCTATTCGAAGCCAACATCAACATGAGTCATTGCCACTGGCATATAATGACAAGATTGTG
GCATTTCTTCGCCAGCCAAACATTTTTGAAATGCTGCAAGAGCGTCAGCCAAGCTTAGCA
AGAAACCACACACTCAGGGAGAAAATCCATTACATTCGGACTGAGGGTAATCACGGGCTT
GAGAAGTTGTCCTGTGATGCGGATCTGGTCATTTTGCTGAGTCTCTTTGAAGAAGAGATT
ATGTCCTACGTCCCCCTGCAGGCTGCCTTCCACCCTGGGTATAGCTTCTCTCCCCGCTGT
TCACCCTGTTCTTCACCTCAGAACTCCCCAGGTTTACAGAGAGCCAGTGCAAGAGCCCCT
TCCCCCTACCGAAGAGACTTTGAGGCCAAGCTCCGCAATTTCTACAGAAAACTGGAAGCC
AAAGGATTTGGTCAGGGTCCGGGGAAAATTAAGCTCATTATTCGCCGGGATCATTTGTTG
GAGGGAACCTTCAATCAGGTGATGGCCTATTCGCGGAAAGAGCTCCAGCGAAACAAGCTC
TACGTCACCTTTGTTGGAGAGGAGGGCCTGGACTACAGTGGCCCCTCGCGGGAGTTCTTC
TTCCTTCTGTCTCAGGAGCTCTTCAACCCTTACTATGGACTCTTTGAGTACTCGGCAAAT
GATACTTACACGGTGCAGATCAGCCCCATGTCCGCATTTGTAGAAAACCATCTTGAGTGG
TTCAGGTTTAGCGGTCGCATCCTGGGTCTGGCTCTGATCCATCAGTACCTTCTTGACGCT
TTCTTCACGAGGCCCTTCTACAAGGCACTCCTGAGACTGCCCTGTGATTTGAGTGACCTG
GAATATTTGGATGAGGAATTCCACCAGAGTTTGCAGTGGATGAAGGACAACAACATCACA
GACATCTTAGACCTCACTTTCACTGTTAATGAAGAGGTTTTTGGACAGGTCACGGAAAGG
GAGTTGAAGTCTGGAGGAGCCAACACACAGGTGACGGAGAAAAACAAGAAGGAGTACATC
GAGCGCATGGTGAAGTGGCGGGTGGAGCGCGGCGTGGTACAGCAGACCGAGGCGCTGGTG
CGCGGCTTCTACGAGGTTGTAGACTCGAGGCTGGTGTCCGTGTTTGATGCCAGGGAGCTG
GAGCTGGTGATAGCTGGCACCGCGGAAATCGACCTAAATGACTGGCGGAATAACACTGAG
TACCGGGGAGGTTACCACGATGGGCATCTTGTGATCCGCTGGTTCTGGGCTGCGGTGGAG
CGCTTCAATAATGAGCAGAGGCTGAGATTACTGCAGTTTGTCACGGGAACATCCAGCGTG
CCCTACGAAGGCTTCGCAGCCCTCCGTGGGAGCAATGGGCTTCGGCGCTTCTGCATAGAG
AAATGGGGGAAAATTACTTCTCTCCCCAGGGCACACACATGCTTCAACCGACTGGATCTT
CCACCGTATCCCTCGTACTCCATGTTGTATGAAAAGCTGTTAACAGCAGTAGAGGAAACC
AGCACCTTTGGACTTGAG

ORF Protein

Cloned ORF protein sequence for pFN21AA0322 Download

>KIAA0322  1606 aa
MLLHLCSVKNLYQNRFLGLAAMASPSRNSQSRRRCKEPLRYSYNPDQFHNMDLRGGPHDG
VTIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIDEV
LSENFLDYKNRGVNGSHRGQIIWKIDASSYFVEPETKICFKYYHGVSGALRATTPSVTVK
NSAAPIFKSIGADETVQGQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSI
FPALPHHGQERRSKIIGNTVNPIWQAEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGK
LSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPDDEEISLSTEPE
SAQIQDSPMNNLMESGSGEPRSEAPESSESWKPEQLGEGSVPDRPGNQSIELSRPAEEAA
VITEAGDQGMVSVGPEGAGELLAQVQKDIQPAPSAEELAEQLDLGEEASALLLEDGEAPA
STKEEPLEEEATTQSRAGREEEEKEQEEEGDVSTLEQGEGRLQLRASVKRKSRPCSLPVS
ELETVIASACGDPETPRTHYIRIHTLLHSMPSAQGGSAAEEEDGAEEESTLKDSSEKDGL
SEVDTVAADPSALEEDREEPEGATPGTAHPGHSGGHFPSLANGAAQDGDTHPSTGSESDS
SPRQGGDHSCEGCDASCCSPSCYSSSCYSTSCYSSSCYSASCYSPSCYNGNRFASHTRFS
SVDSAKISESTVFSSQDDEEEENSAFESVPDSMQSPELDPESTNGAGPWQDELAAPSGHV
ERSPEGLESPVAGPSNRREGECPILHNSQPVSQLPSLRPEHHHYPTIDEPLPPNWEARID
SHGRVFYVDHVNRTTTWQRPTAAATPDGMRRSGSIQQMEQLNRRYQNIQRTIATERSEED
SGSQSCEQAPAGGGGGGGSDSEAESSQSSLDLRREGSLSPVNSQKITLLLQSPAVKFITN
PEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLEL
PRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEAS
EVSRNRGASLLARPGHSLVAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLA
RNHTLREKIHYIRTEGNHGLEKLSCDADLVILLSLFEEEIMSYVPLQAAFHPGYSFSPRC
SPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLL
EGTFNQVMAYSRKELQRNKLYVTFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSAN
DTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDL
EYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYI
ERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTE
YRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIE
KWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AA0322 Download

>pFN21AA0322  9523 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGCTGCTGCACCTGTGTAGTGTGAAGAATCTGTA
CCAGAACAGGTTTTTAGGCCTGGCCGCCATGGCGTCTCCTTCTAGAAACTCCCAGAGCCG
ACGCCGGTGCAAGGAGCCGCTCCGATACAGCTACAACCCCGACCAGTTCCACAACATGGA
CCTCAGGGGCGGCCCCCACGATGGCGTCACCATTCCCCGCTCCACCAGCGACACTGACCT
GGTCACCTCGGACAGCCGCTCCACGCTCATGGTCAGCAGCTCCTACTATTCCATCGGGCA
CTCTCAGGACCTGGTCATCCACTGGGACATAAAGGAGGAAGTGGACGCTGGGGACTGGAT
TGGCATGTACCTCATTGATGAGGTCTTGTCCGAAAACTTTCTGGACTATAAAAACCGTGG
AGTCAATGGTTCTCATCGGGGCCAGATCATCTGGAAGATCGATGCCAGCTCGTACTTTGT
GGAACCTGAAACTAAGATCTGCTTCAAATACTACCATGGAGTGAGTGGGGCCCTGCGAGC
AACCACCCCCAGTGTCACGGTCAAAAACTCGGCAGCTCCTATTTTTAAAAGCATTGGTGC
TGATGAGACCGTCCAAGGACAAGGAAGTCGGAGGCTGATCAGCTTCTCTCTCTCAGATTT
CCAAGCCATGGGGTTGAAGAAAGGGATGTTTTTCAACCCAGACCCTTATCTGAAGATTTC
CATTCAGCCTGGGAAACACAGCATCTTCCCCGCCCTCCCTCACCATGGACAGGAGAGGAG
ATCCAAGATCATAGGCAACACCGTGAACCCCATCTGGCAGGCCGAGCAATTCAGTTTTGT
GTCCTTGCCCACTGACGTGCTGGAAATTGAGGTGAAGGACAAGTTTGCCAAGAGCCGCCC
CATCATCAAGCGCTTCTTGGGAAAGCTGTCGATGCCCGTTCAAAGACTCCTGGAGAGACA
CGCCATAGGGGATAGGGTGGTCAGCTACACACTTGGCCGCAGGCTTCCAACAGATCATGT
GAGTGGACAGCTGCAATTCCGATTTGAGATCACTTCCTCCATCCACCCAGATGATGAGGA
GATTTCCCTGAGTACCGAGCCTGAGTCAGCCCAAATTCAGGACAGCCCCATGAACAACCT
GATGGAAAGCGGCAGTGGGGAACCTCGGTCTGAGGCACCAGAGTCCTCTGAGAGCTGGAA
GCCAGAGCAGCTGGGTGAGGGCAGTGTCCCCGATCGTCCAGGGAACCAAAGCATAGAGCT
TTCCAGACCAGCTGAGGAAGCAGCAGTCATCACGGAGGCAGGAGACCAGGGCATGGTCTC
TGTGGGACCTGAAGGGGCTGGGGAGCTCCTGGCCCAGGTGCAAAAGGACATCCAGCCTGC
CCCCAGTGCAGAAGAGCTGGCCGAGCAGCTGGACCTGGGTGAGGAGGCATCAGCACTGCT
GCTGGAAGACGGTGAAGCCCCAGCCAGCACCAAGGAGGAGCCCTTGGAGGAGGAAGCAAC
GACCCAGAGCCGGGCTGGAAGGGAAGAAGAGGAGAAGGAGCAGGAGGAGGAGGGAGATGT
GTCCACCCTGGAGCAGGGAGAGGGCAGGCTGCAGCTGCGGGCCTCGGTGAAGAGAAAAAG
CAGGCCCTGCTCCTTGCCTGTGTCCGAGCTGGAGACGGTGATCGCGTCAGCCTGCGGGGA
CCCCGAGACCCCGCGGACACACTACATCCGCATCCACACCCTGCTGCACAGCATGCCCTC
CGCCCAGGGCGGCAGCGCGGCAGAGGAGGAGGACGGCGCGGAGGAGGAGTCCACCCTCAA
GGACTCCTCGGAGAAGGATGGGCTCAGCGAGGTGGACACGGTGGCCGCTGACCCGTCTGC
CCTGGAAGAGGACAGAGAAGAGCCCGAGGGGGCTACTCCAGGCACGGCGCACCCTGGCCA
CTCCGGGGGCCACTTCCCCAGCCTGGCCAATGGCGCGGCCCAGGATGGCGACACGCACCC
CAGCACCGGGAGCGAGAGCGACTCCAGCCCCAGGCAAGGCGGGGACCACAGTTGCGAGGG
CTGTGACGCGTCCTGCTGCAGCCCCTCGTGCTACAGCTCCTCGTGCTACAGCACGTCCTG
CTACAGCAGCTCGTGCTACAGCGCCTCGTGCTACAGCCCCTCCTGCTACAACGGCAACAG
GTTCGCCAGCCACACGCGCTTCTCCTCCGTGGACAGCGCCAAGATCTCCGAGAGCACGGT
CTTCTCCTCGCAAGACGACGAGGAGGAGGAGAACAGCGCGTTCGAGTCGGTACCCGACTC
CATGCAGAGCCCTGAGCTGGACCCGGAGTCCACGAACGGCGCTGGGCCGTGGCAAGACGA
GCTGGCCGCCCCTAGCGGGCACGTGGAAAGAAGCCCGGAAGGTCTGGAATCCCCCGTGGC
AGGTCCAAGCAATCGGAGAGAAGGTGAATGTCCTATACTCCATAATTCCCAGCCAGTAAG
CCAGCTTCCTTCCCTGAGGCCTGAACATCATCACTACCCAACAATCGATGAGCCTCTTCC
ACCAAACTGGGAAGCTCGAATTGACAGCCACGGGCGGGTCTTTTATGTGGACCACGTGAA
CCGCACAACCACCTGGCAGCGTCCGACGGCAGCAGCCACCCCGGATGGCATGCGGAGATC
GGGGTCCATCCAGCAGATGGAGCAACTCAACAGGCGGTATCAAAACATTCAGCGAACCAT
TGCAACAGAGAGGTCCGAAGAAGATTCTGGCAGCCAAAGCTGCGAGCAAGCCCCAGCAGG
AGGAGGCGGAGGTGGAGGGAGTGACTCAGAAGCCGAATCTTCCCAGTCCAGCTTAGATCT
AAGGAGAGAGGGGTCACTTTCTCCAGTGAACTCACAAAAAATCACCTTGCTGCTGCAGTC
CCCAGCGGTCAAGTTCATCACCAACCCCGAGTTCTTCACTGTGCTACATGCCAATTATAG
TGCCTACCGAGTCTTCACCAGTAGCACCTGCTTAAAGCACATGATTCTGAAAGTCCGACG
GGATGCTCGCAATTTTGAACGCTACCAGCACAACCGGGACTTGGTGAATTTCATCAACAT
GTTCGCAGACACTCGGCTGGAACTGCCCCGGGGCTGGGAGATCAAAACGGACCAGCAGGG
AAAGTCTTTTTTCGTGGACCACAACAGTCGAGCTACCACTTTCATTGACCCCCGAATCCC
TCTTCAGAACGGTCGTCTTCCCAATCATCTAACTCACCGACAGCACCTCCAGAGGCTCCG
AAGTTACAGCGCTGGAGAGGCCTCAGAAGTTTCTAGAAACAGAGGAGCCTCTTTACTGGC
CAGGCCAGGACACAGCTTAGTAGCTGCTATTCGAAGCCAACATCAACATGAGTCATTGCC
ACTGGCATATAATGACAAGATTGTGGCATTTCTTCGCCAGCCAAACATTTTTGAAATGCT
GCAAGAGCGTCAGCCAAGCTTAGCAAGAAACCACACACTCAGGGAGAAAATCCATTACAT
TCGGACTGAGGGTAATCACGGGCTTGAGAAGTTGTCCTGTGATGCGGATCTGGTCATTTT
GCTGAGTCTCTTTGAAGAAGAGATTATGTCCTACGTCCCCCTGCAGGCTGCCTTCCACCC
TGGGTATAGCTTCTCTCCCCGCTGTTCACCCTGTTCTTCACCTCAGAACTCCCCAGGTTT
ACAGAGAGCCAGTGCAAGAGCCCCTTCCCCCTACCGAAGAGACTTTGAGGCCAAGCTCCG
CAATTTCTACAGAAAACTGGAAGCCAAAGGATTTGGTCAGGGTCCGGGGAAAATTAAGCT
CATTATTCGCCGGGATCATTTGTTGGAGGGAACCTTCAATCAGGTGATGGCCTATTCGCG
GAAAGAGCTCCAGCGAAACAAGCTCTACGTCACCTTTGTTGGAGAGGAGGGCCTGGACTA
CAGTGGCCCCTCGCGGGAGTTCTTCTTCCTTCTGTCTCAGGAGCTCTTCAACCCTTACTA
TGGACTCTTTGAGTACTCGGCAAATGATACTTACACGGTGCAGATCAGCCCCATGTCCGC
ATTTGTAGAAAACCATCTTGAGTGGTTCAGGTTTAGCGGTCGCATCCTGGGTCTGGCTCT
GATCCATCAGTACCTTCTTGACGCTTTCTTCACGAGGCCCTTCTACAAGGCACTCCTGAG
ACTGCCCTGTGATTTGAGTGACCTGGAATATTTGGATGAGGAATTCCACCAGAGTTTGCA
GTGGATGAAGGACAACAACATCACAGACATCTTAGACCTCACTTTCACTGTTAATGAAGA
GGTTTTTGGACAGGTCACGGAAAGGGAGTTGAAGTCTGGAGGAGCCAACACACAGGTGAC
GGAGAAAAACAAGAAGGAGTACATCGAGCGCATGGTGAAGTGGCGGGTGGAGCGCGGCGT
GGTACAGCAGACCGAGGCGCTGGTGCGCGGCTTCTACGAGGTTGTAGACTCGAGGCTGGT
GTCCGTGTTTGATGCCAGGGAGCTGGAGCTGGTGATAGCTGGCACCGCGGAAATCGACCT
AAATGACTGGCGGAATAACACTGAGTACCGGGGAGGTTACCACGATGGGCATCTTGTGAT
CCGCTGGTTCTGGGCTGCGGTGGAGCGCTTCAATAATGAGCAGAGGCTGAGATTACTGCA
GTTTGTCACGGGAACATCCAGCGTGCCCTACGAAGGCTTCGCAGCCCTCCGTGGGAGCAA
TGGGCTTCGGCGCTTCTGCATAGAGAAATGGGGGAAAATTACTTCTCTCCCCAGGGCACA
CACATGCTTCAACCGACTGGATCTTCCACCGTATCCCTCGTACTCCATGTTGTATGAAAA
GCTGTTAACAGCAGTAGAGGAAACCAGCACCTTTGGACTTGAGGTTTAAACGAATTCGGG
CTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTA
ACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAAC
CCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACC
ACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTA
TTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATG
TTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGT
GGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCC
CAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATG
GTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTA
TTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTT
CAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCC
TTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAA
GATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGT
AAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTT
CTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGC
ATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACG
GATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCG
GCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAAC
ATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCA
AACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTA
ACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGAT
AAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAA
TCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAG
CCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAAT
AGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGAC
CAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAAT
CAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTA
AAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAA
ATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTC
CCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGT
CCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCA
GTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCG
ACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTAT
CGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTA
CAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCT
GCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAAC
AAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAA
AAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAA
ACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTT
TATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCAT
GAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCA
TAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTG
TTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACA
ATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGC
AGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT