(Circular) (Six-base) MAPSORT of: pFN21AA0322.seq Check: 1242 from: 1 to: 9523 pFN21AA0322 9523 bp With 182 enzymes: SgfI * October 20, 2009 10:33 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4955 4955 Size: 9523 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 7333 278 Size: 53 83 186 936 5797 2468 Fragments arranged by size: 5797 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 6405 6866 1937 Size: 4468 461 4594 Fragments arranged by size: 4594 4468 461 Acc65I G'GTAC_C Cuts at: 4249 6844 4249 Size: 2595 6928 AclI AA'CG_TT Cuts at: 1563 7650 8023 1563 Size: 6087 373 3063 Fragments arranged by size: 6087 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2711 3330 5147 7586 8970 9238 1632 Size: 1079 619 1817 2439 1384 268 1917 Fragments arranged by size: 2439 1917 1817 1384 1079 619 268 AfeI AGC'GCT Cuts at: 2891 5230 6566 2891 Size: 2339 1336 5848 Fragments arranged by size: 5848 2339 1336 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 9326 AflIII A'CryG_T Cuts at: 1203 4026 5037 8422 9399 1203 Size: 2823 1011 3385 977 1327 Fragments arranged by size: 3385 2823 1327 1011 977 AgeI A'CCGG_T Cuts at: 8354 8354 Size: 9523 AhdI GACnn_n'nnGTC Cuts at: 4285 8252 4285 Size: 3967 5556 AleI CACnn'nnGTG Cuts at: 1166 2834 6293 1166 Size: 1668 3459 4396 Fragments arranged by size: 4396 3459 1668 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 6739 1184 Size: 5555 3968 AlwNI CAG_nnn'CTG Cuts at: 2290 3606 6586 7266 8838 2290 Size: 1316 2980 680 1572 2975 Fragments arranged by size: 2980 2975 1572 1316 680 ApaI G_GGCC'C Cuts at: 2512 2512 Size: 9523 ApaLI G'TGCA_C Cuts at: 7580 8736 7580 Size: 1156 8367 ApoI r'AATT_y Cuts at: 1493 3093 5026 6161 6832 7058 7210 9319 1493 Size: 1600 1933 1135 671 226 152 2109 1697 Fragments arranged by size: 2109 1933 1697 1600 1135 671 226 152 AseI AT'TA_AT Cuts at: 160 1032 8077 160 Size: 872 7045 1606 Fragments arranged by size: 7045 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9523 AvaI C'yCGr_G Cuts at: 1870 1943 3662 3863 4886 5067 6409 6848 1870 Size: 73 1719 201 1023 181 1342 439 4545 Fragments arranged by size: 4545 1719 1342 1023 439 201 181 73 BamHI G'GATC_C Cuts at: 6853 7220 6853 Size: 367 9156 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3154 4249 6461 6844 8299 Size: 325 217 627 1367 1095 2212 383 1455 Cuts at: 8299 618 Size: 1842 Fragments arranged by size: 2212 1842 1455 1367 1095 627 383 325 217 BanII G_rGCy'C Cuts at: 1391 2512 3327 3462 3804 3864 4271 5662 5829 Size: 1121 815 135 342 60 407 1391 167 Cuts at: 5829 5925 6842 1391 Size: 96 917 4072 Fragments arranged by size: 4072 1391 1121 917 815 407 342 167 135 96 60 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3432 4192 4924 5168 928 Size: 971 1533 760 732 244 5283 Fragments arranged by size: 5283 1533 971 760 732 244 BbvCI CC'TCA_GC Cuts at: 3252 3252 Size: 9523 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2088 2122 4123 4157 7714 Size: 34 50 34 67 34 2001 34 3557 Cuts at: 7714 7748 1903 Size: 34 3678 Fragments arranged by size: 3678 3557 2001 67 50 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6762 7415 8625 6762 Size: 653 1210 7660 Fragments arranged by size: 7660 1210 653 BclI T'GATC_A Cuts at: 1548 2616 1548 Size: 1068 8455 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2069 8134 136 Size: 107 122 71 631 605 397 6065 1525 Fragments arranged by size: 6065 1525 631 605 397 122 107 71 BglII A'GATC_T Cuts at: 2475 4182 4795 9518 2475 Size: 1707 613 4723 2480 Fragments arranged by size: 4723 2480 1707 613 BlpI GC'TnA_GC Cuts at: 3803 5419 6940 3803 Size: 1616 1521 6386 Fragments arranged by size: 6386 1616 1521 Bme1580I G_kGCm'C Cuts at: 1790 2280 2512 4341 6627 6718 7584 8740 1790 Size: 490 232 1829 2286 91 866 1156 2573 Fragments arranged by size: 2573 2286 1829 1156 866 490 232 91 BmgBI CAC'GTC Cuts at: 1318 2836 4074 1318 Size: 1518 1238 6767 Fragments arranged by size: 6767 1518 1238 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2311 2523 3357 Size: 416 53 83 399 24 894 212 834 Cuts at: 3357 4516 4777 7254 8212 442 Size: 1159 261 2477 958 1753 Fragments arranged by size: 2477 1753 1159 958 894 834 416 399 261 212 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9523 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3997 3997 Size: 9523 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2951 3569 3650 3861 3967 4806 5192 5253 Size: 1712 618 81 211 106 839 386 61 Cuts at: 5253 5813 6297 6464 8183 1239 Size: 560 484 167 1719 2579 Fragments arranged by size: 2579 1719 1712 839 618 560 484 386 211 167 106 81 61 Bpu10I CC'TnA_GC Cuts at: 3252 4272 3252 Size: 1020 8503 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3761 5504 6038 6839 7648 8513 8811 3761 Size: 1743 534 801 809 865 298 4473 Fragments arranged by size: 4473 1743 865 809 801 534 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2579 3276 3301 3659 8186 882 Size: 617 1080 697 25 358 4527 2219 Fragments arranged by size: 4527 2219 1080 697 617 358 25 BsaAI yAC'GTr Cuts at: 493 1204 4342 4555 7314 493 Size: 711 3138 213 2759 2702 Fragments arranged by size: 3138 2759 2702 711 213 BsaBI GATnn'nnATC Cuts at: 5505 6528 9517 5505 Size: 1023 2989 5511 Fragments arranged by size: 5511 2989 1023 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2072 2101 2184 7330 Size: 53 83 186 936 539 29 83 5146 Cuts at: 7330 7712 8347 275 Size: 382 635 1451 Fragments arranged by size: 5146 1451 936 635 539 382 186 83 83 53 29 BsaWI w'CCGG_w Cuts at: 7956 8354 8628 8775 9186 7956 Size: 398 274 147 411 8293 Fragments arranged by size: 8293 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2222 2252 3752 3782 4103 Size: 30 165 30 819 30 1500 30 321 Cuts at: 4103 4133 4387 4417 5190 5220 6257 6287 1178 Size: 30 254 30 773 30 1037 30 4414 Fragments arranged by size: 4414 1500 1037 819 773 321 254 165 30 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2770 3071 3416 3470 3482 3524 3539 3542 Size: 1584 301 345 54 12 42 15 3 Cuts at: 3542 3758 3761 3775 3776 3779 4048 4153 4195 Size: 216 3 14 1 3 269 105 42 Cuts at: 4195 4235 4238 4241 4755 5277 5879 6294 1186 Size: 40 3 3 514 522 602 415 4415 Fragments arranged by size: 4415 1584 602 522 514 415 345 301 269 216 105 54 42 42 40 15 14 12 3 3 3 3 3 1 BseYI C'CCAG_C Cuts at: 2327 3190 3317 3439 3917 3959 4301 4428 4732 Size: 863 127 122 478 42 342 127 304 Cuts at: 4732 4861 5073 8726 9222 2327 Size: 129 212 3653 496 2628 Fragments arranged by size: 3653 2628 863 496 478 342 304 212 129 127 127 122 42 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1997 3387 3689 5999 1163 Size: 573 261 1390 302 2310 4687 Fragments arranged by size: 4687 2310 1390 573 302 261 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3214 5173 6040 6973 7734 8762 Size: 275 186 1213 1959 867 933 761 1028 Cuts at: 8762 1540 Size: 2301 Fragments arranged by size: 2301 1959 1213 1028 933 867 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3327 4196 5829 5925 7584 7669 8740 3327 Size: 869 1633 96 1659 85 1071 4110 Fragments arranged by size: 4110 1659 1633 1071 869 96 85 BsmI GAATG_Cn' Cuts at: 7034 7127 7034 Size: 93 9430 BsmBI CGTCTCn'nnnn_ Cuts at: 2079 3626 2079 Size: 1547 7976 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2280 2512 3327 3462 3804 3864 4196 Size: 399 490 232 815 135 342 60 332 Cuts at: 4196 4271 4341 5662 5829 5925 6627 6718 6842 Size: 75 70 1321 167 96 702 91 124 Cuts at: 6842 7584 7669 8740 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1321 1071 815 742 702 490 399 342 332 232 167 135 124 96 91 85 75 70 60 BspEI T'CCGG_A Cuts at: 9186 9186 Size: 9523 BspHI T'CATG_A Cuts at: 7412 9142 7412 Size: 1730 7793 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4370 4955 6878 844 Size: 3526 585 1923 3489 Fragments arranged by size: 3526 3489 1923 585 BsrBI CCG'CTC Cuts at: 1394 1823 2119 2199 2238 7410 1394 Size: 429 296 80 39 5172 3507 Fragments arranged by size: 5172 3507 429 296 80 39 BsrDI GCAATG_nn' Cuts at: 65 1248 4677 5333 6664 8018 8192 65 Size: 1183 3429 656 1331 1354 174 1396 Fragments arranged by size: 3429 1396 1354 1331 1183 656 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2103 8167 8354 1700 Size: 110 293 6064 187 2869 Fragments arranged by size: 6064 2869 293 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 9523 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9523 BssSI C'ACGA_G Cuts at: 4045 4060 4090 4105 6091 7583 8595 4045 Size: 15 30 15 1986 1492 1012 4973 Fragments arranged by size: 4973 1986 1492 1012 30 15 15 BstAPI GCAn_nnn'nTGC Cuts at: 794 3606 3712 4077 4092 794 Size: 2812 106 365 15 6225 Fragments arranged by size: 6225 2812 365 106 15 BstBI TT'CG_AA Cuts at: 5311 8327 5311 Size: 3016 6507 BstEII G'GTnAC_C Cuts at: 1184 2221 6515 1184 Size: 1037 4294 4192 Fragments arranged by size: 4294 4192 1037 BstXI CCAn_nnnn'nTGG Cuts at: 2248 4506 6442 2248 Size: 2258 1936 5329 Fragments arranged by size: 5329 2258 1936 BstYI r'GATC_y Cuts at: 1972 2475 2759 4182 4795 5506 6739 6853 7220 Size: 503 284 1423 613 711 1233 114 367 Cuts at: 7220 7605 7622 9074 9160 9172 9518 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1423 1233 711 613 503 385 367 346 284 114 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2162 4454 2162 Size: 2292 7231 BtgI C'CryG_G Cuts at: 513 1065 2067 2395 2494 2646 2743 3671 4167 Size: 552 1002 328 99 152 97 928 496 Cuts at: 4167 4307 4374 4528 6465 6649 513 Size: 140 67 154 1937 184 3387 Fragments arranged by size: 3387 1937 1002 928 552 496 328 184 154 152 140 99 97 67 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 8784 Fragments arranged by size: 8784 683 56 BtsI GCAGTG_nn' Cuts at: 3139 3208 3413 6185 7039 7833 7853 3139 Size: 69 205 2772 854 794 20 4809 Fragments arranged by size: 4809 2772 854 794 205 69 20 ClaI AT'CG_AT Cuts at: 1554 2439 4485 1554 Size: 885 2046 6592 Fragments arranged by size: 6592 2046 885 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 5125 5160 529 Size: 35 4561 35 4892 Fragments arranged by size: 4892 4561 35 35 DraI TTT'AAA Cuts at: 2566 6827 2566 Size: 4261 5262 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2604 8530 809 Size: 507 1288 5926 1802 Fragments arranged by size: 5926 1802 1288 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2061 2974 3378 Size: 54 1425 50 275 18 231 913 404 Cuts at: 3378 3822 3895 3924 4323 5277 6970 7859 9488 Size: 444 73 29 399 954 1693 889 1629 Cuts at: 9488 8 Size: 43 Fragments arranged by size: 1693 1629 1425 954 913 889 444 404 399 275 231 73 54 50 43 29 18 EagI C'GGCC_G Cuts at: 1537 1812 6970 1537 Size: 275 5158 4090 Fragments arranged by size: 5158 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3366 3501 3584 3840 3852 4500 5166 5532 Size: 2013 135 83 256 12 648 666 366 Cuts at: 5532 5671 5930 6231 7453 1353 Size: 139 259 301 1222 3423 Fragments arranged by size: 3423 2013 1222 666 648 366 301 259 256 139 135 83 12 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3709 4761 6505 8110 8484 8630 Size: 1064 138 2120 1052 1744 1605 374 146 Cuts at: 8630 387 Size: 1280 Fragments arranged by size: 2120 1744 1605 1280 1064 1052 374 146 138 EcoICRI GAG'CTC Cuts at: 3325 5827 5923 3325 Size: 2502 96 6925 Fragments arranged by size: 6925 2502 96 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2711 2951 3330 3569 3650 3861 3967 Size: 393 1079 240 379 239 81 211 106 Cuts at: 3967 4806 5147 5192 5253 5813 6297 6464 7586 Size: 839 341 45 61 560 484 167 1122 Cuts at: 7586 8183 8970 9238 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1122 1079 839 787 597 560 484 393 379 341 268 240 239 211 167 106 81 61 45 EcoNI CCTnn'n_nnAGG Cuts at: 3332 3398 3551 3845 3332 Size: 66 153 294 9010 Fragments arranged by size: 9010 294 153 66 EcoO109I rG'GnC_Cy Cuts at: 2287 2508 2509 3305 3603 3658 5869 6096 2287 Size: 221 1 796 298 55 2211 227 5714 Fragments arranged by size: 5714 2211 796 298 227 221 55 1 EcoRI G'AATT_C Cuts at: 6161 6832 7210 6161 Size: 671 378 8474 Fragments arranged by size: 8474 671 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2497 2911 5679 2497 Size: 414 2768 6341 Fragments arranged by size: 6341 2768 414 FspI TGC'GCA Cuts at: 8029 8029 Size: 9523 HaeII r_GCGC'y Cuts at: 1678 2893 4104 4179 4302 5232 6380 6568 6673 Size: 1215 1211 75 123 930 1148 188 105 Cuts at: 6673 8670 1678 Size: 1997 2531 Fragments arranged by size: 2531 1997 1215 1211 1148 930 188 123 105 75 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2222 2254 2351 2383 2432 Size: 32 135 32 650 32 97 32 49 Cuts at: 2432 2464 2744 2751 2776 2783 3012 3044 3068 Size: 32 280 7 25 7 229 32 24 Cuts at: 3068 3100 3392 3424 3752 3784 4271 4303 5190 Size: 32 292 32 328 32 487 32 887 Cuts at: 5190 5222 5477 5509 5531 5563 6257 6289 8164 Size: 32 255 32 22 32 694 32 1875 Cuts at: 8164 8196 8238 8270 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1875 887 694 650 487 328 292 280 255 229 135 97 49 42 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 25 24 22 7 7 HincII GTy'rAC Cuts at: 669 1657 1938 6786 6867 7113 669 Size: 988 281 4848 81 246 3079 Fragments arranged by size: 4848 3079 988 281 246 81 HindIII A'AGCT_T Cuts at: 748 5416 748 Size: 4668 4855 HpaI GTT'AAC Cuts at: 6786 7113 6786 Size: 327 9196 KpnI G_GTAC'C Cuts at: 4253 6848 4253 Size: 2595 6928 MfeI C'AATT_G Cuts at: 7122 7122 Size: 9523 MluI A'CGCG_T Cuts at: 4026 9399 4026 Size: 5373 4150 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2588 3011 4607 4999 5763 5839 8637 8821 Size: 1283 423 1596 392 764 76 2798 184 Cuts at: 8821 1305 Size: 2007 Fragments arranged by size: 2798 2007 1596 1283 764 423 392 184 76 MscI TGG'CCA Cuts at: 10 64 1489 1832 3897 3926 5279 10 Size: 54 1425 343 2065 29 1353 4254 Fragments arranged by size: 4254 2065 1425 1353 343 54 29 MslI CAynn'nnrTG Cuts at: 518 1166 2246 2834 3000 3048 5036 5325 6293 Size: 648 1080 588 166 48 1988 289 968 Cuts at: 6293 6721 7481 7840 7999 518 Size: 428 760 359 159 2042 Fragments arranged by size: 2042 1988 1080 968 760 648 588 428 359 289 166 159 48 MspA1I CmG'CkG Cuts at: 1606 3010 3190 3251 3388 3574 3673 3827 4865 Size: 1404 180 61 137 186 99 154 1038 Cuts at: 4865 5601 6467 6543 6939 7616 8764 9009 1606 Size: 736 866 76 396 677 1148 245 2120 Fragments arranged by size: 2120 1404 1148 1038 866 736 677 396 245 186 180 154 137 99 76 61 NcoI C'CATG_G Cuts at: 513 1065 2067 2494 2646 2743 513 Size: 552 1002 427 152 97 7293 Fragments arranged by size: 7293 1002 552 427 152 97 NdeI CA'TA_TG Cuts at: 387 387 Size: 9523 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9523 NotI GC'GGCC_GC Cuts at: 6970 6970 Size: 9523 NspI r_CATG'y Cuts at: 1132 1229 2347 3715 4612 4910 5041 6726 6881 Size: 97 1118 1368 897 298 131 1685 155 Cuts at: 6881 8426 1132 Size: 1545 2229 Fragments arranged by size: 2229 1685 1545 1368 1118 897 298 155 131 97 PasI CC'CwG_GG Cuts at: 1348 3724 5579 6711 1348 Size: 2376 1855 1132 4160 Fragments arranged by size: 4160 2376 1855 1132 PciI A'CATG_T Cuts at: 5037 8422 5037 Size: 3385 6138 PflMI CCAn_nnn'nTGG Cuts at: 1108 2155 2647 3292 4636 1108 Size: 1047 492 645 1344 5995 Fragments arranged by size: 5995 1344 1047 645 492 PfoI T'CCnGG_A Cuts at: 2928 2928 Size: 9523 PmeI GTTT'AAAC Cuts at: 6827 6827 Size: 9523 PmlI CAC'GTG Cuts at: 4342 4555 4342 Size: 213 9310 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 7592 7624 9105 9137 7592 Size: 32 1481 32 7978 Fragments arranged by size: 7978 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2287 3305 3658 2287 Size: 1018 353 8152 Fragments arranged by size: 8152 1018 353 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 9523 PsiI TTA'TAA Cuts at: 7093 7093 Size: 9523 PspOMI G'GGCC_C Cuts at: 2508 2508 Size: 9523 PspXI vC'TCGA_Gb Cuts at: 6409 6409 Size: 9523 PstI C_TGCA'G Cuts at: 830 3573 4040 4857 5565 6600 6875 830 Size: 2743 467 817 708 1035 275 3478 Fragments arranged by size: 3478 2743 1035 817 708 467 275 PvuI CG_AT'CG Cuts at: 2001 3214 2001 Size: 1213 8310 PvuII CAG'CTG Cuts at: 3010 3190 3251 3388 3574 3010 Size: 180 61 137 186 8959 Fragments arranged by size: 8959 186 180 137 61 SacI G_AGCT'C Cuts at: 3327 5829 5925 3327 Size: 2502 96 6925 Fragments arranged by size: 6925 2502 96 SacII CC_GC'GG Cuts at: 3674 6468 3674 Size: 2794 6729 SalI G'TCGA_C Cuts at: 1936 6865 1936 Size: 4929 4594 SanDI GG'GwC_CC Cuts at: 3658 3658 Size: 9523 SapI GCTCTTCn'nnn_ Cuts at: 3366 3852 5930 3366 Size: 486 2078 6959 Fragments arranged by size: 6959 2078 486 SbfI CC_TGCA'GG Cuts at: 5565 6875 5565 Size: 1310 8213 ScaI AGT'ACT Cuts at: 5954 7771 9483 5954 Size: 1817 1712 5994 Fragments arranged by size: 5994 1817 1712 SexAI A'CCwGG_T Cuts at: 2217 2289 2217 Size: 72 9451 SfcI C'TryA_G Cuts at: 826 1045 3569 4036 4051 4066 4081 4096 4111 Size: 219 2524 467 15 15 15 15 15 Cuts at: 4111 4853 5561 5707 5878 6596 6871 8006 8687 Size: 742 708 146 171 718 275 1135 681 Cuts at: 8687 8878 826 Size: 191 1471 Fragments arranged by size: 2524 1471 1135 742 718 708 681 467 275 219 191 171 146 15 15 15 15 15 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9523 SgrAI Cr'CCGG_yG Cuts at: 1700 2103 1700 Size: 403 9120 SmaI CCC'GGG Cuts at: 1872 5069 6850 1872 Size: 3197 1781 4545 Fragments arranged by size: 4545 3197 1781 SmlI C'TyrA_G Cuts at: 820 1017 1943 3776 5483 6017 6409 6818 7627 Size: 197 926 1833 1707 534 392 409 809 Cuts at: 7627 8528 8790 820 Size: 901 262 1553 Fragments arranged by size: 1833 1707 1553 926 901 809 534 409 392 262 197 SnaBI TAC'GTA Cuts at: 493 7314 493 Size: 6821 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 9523 SphI G_CATG'C Cuts at: 1132 3715 4612 6881 1132 Size: 2583 897 2269 3774 Fragments arranged by size: 3774 2583 2269 897 SspI AAT'ATT Cuts at: 5 52 6149 7447 5 Size: 47 6097 1298 2081 Fragments arranged by size: 6097 2081 1298 47 StuI AGG'CCT Cuts at: 1517 2058 4459 5240 1517 Size: 541 2401 781 5800 Fragments arranged by size: 5800 2401 781 541 StyI C'CwwG_G Cuts at: 513 1065 2067 2494 2593 2646 2743 3450 3462 Size: 552 1002 427 99 53 97 707 12 Cuts at: 3462 6962 513 Size: 3500 3074 Fragments arranged by size: 3500 3074 1002 707 552 427 99 97 53 12 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3060 3135 3182 3386 6386 7550 7567 Size: 1255 1480 75 47 204 3000 1164 17 Cuts at: 7567 7720 7905 8350 325 Size: 153 185 445 1498 Fragments arranged by size: 3000 1498 1480 1255 1164 445 204 185 153 75 47 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5952 6318 7769 Size: 274 80 33 51 1443 3975 366 1451 Cuts at: 7769 9481 96 Size: 1712 138 Fragments arranged by size: 3975 1712 1451 1443 366 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3669 3701 4387 4419 9483 9515 3669 Size: 32 686 32 5064 32 3677 Fragments arranged by size: 5064 3677 686 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2219 2291 1316 Size: 903 72 8548 Fragments arranged by size: 8548 903 72 XbaI T'CTAG_A Cuts at: 2081 5252 6859 2081 Size: 3171 1607 4745 Fragments arranged by size: 4745 3171 1607 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2501 2836 3491 4633 2501 Size: 335 655 1142 7391 Fragments arranged by size: 7391 1142 655 335 XhoI C'TCGA_G Cuts at: 1943 6409 1943 Size: 4466 5057 XmaI C'CCGG_G Cuts at: 1870 5067 6848 1870 Size: 3197 1781 4545 Fragments arranged by size: 4545 3197 1781 XmnI GAAnn'nnTTC Cuts at: 2376 7652 2376 Size: 5276 4247 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 7331 276 Size: 53 83 186 936 5797 2468 Fragments arranged by size: 5797 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PsrI RsrII SfiI SfoI SrfI SwaI