PCCA ORF clone pF1KE2601

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Flexi ORF Clone pF1KE2601

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Clone info.

Species

human

Clone Name

pF1KE2601 Sequence (with vector) Restriction Map

Product ID

FXC25051

HaloTag Clone PID

ORF Sequence

DNA sequence (2184 bp)
Protein sequence (728 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pF1K Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE2601

Symbol

PCCA

Description

propionyl-CoA carboxylase alpha subunit, transcript variant 1

Original Clone ID

cp01697

Note

We obtained this clone using the E.coli strain DH10B at 25 deg C.

Features of the cloned ORF sequence

Description

Length: 2184 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 728 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS9496.2 0 100.0 PCCA CCDS45065.1 0 96.4 PCCA CCDS53878.1 0 93.5 PCCA CCDS3241.1 1.7e-105 40.3 MCCC1 CCDS8152.1 1.1e-83 45.0 PC The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR005481 63 171 PF00289 Carbamoyl-phosphate synthase IPR005479 176 384 PF02786 Carbamoyl-phosphate synthetase IPR005482 397 504 PF02785 Biotin carboxylase IPR000089 662 727 PF00364 Biotin/lipoyl attachment HMMSmart IPR005482 397 505 SM00878 Biotin carboxylase ProfileScan IPR011764 62 509 PS50979 Biotin carboxylation domain IPR011761 181 378 PS50975 ATP-grasp fold IPR000089 660 727 PS50968 Biotin/lipoyl attachment
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_000273 728 232000,606054 100.0 100.0 XP_016876098 695 232000,606054 99.7 95.5 NP_001121164 702 232000,606054 96.4 100.0 XP_005254116 640 232000,606054 100.0 87.0 NP_001171475 681 232000,606054 93.5 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pF1KE2601 Download

>KIEE2601  2184 bp
ATGGCGGGGTTCTGGGTCGGGACAGCACCGCTGGTCGCTGCCGGACGGCGTGGGCGGTGG
CCGCCGCAGCAGCTGATGCTGAGCGCGGCGCTGCGGACCCTGAAGCATGTTCTGTACTAT
TCAAGACAGTGCTTAATGGTGTCCCGTAATCTTGGTTCAGTGGGATATGATCCTAATGAA
AAAACTTTTGATAAAATTCTTGTTGCTAATAGAGGAGAAATTGCATGTCGGGTTATTAGA
ACTTGCAAGAAGATGGGCATTAAGACAGTTGCCATCCACAGTGATGTTGATGCTAGTTCT
GTTCATGTGAAAATGGCGGATGAGGCTGTCTGTGTTGGCCCAGCTCCCACCAGTAAAAGC
TACCTCAACATGGATGCCATCATGGAAGCCATTAAGAAAACCAGGGCCCAAGCTGTACAT
CCAGGTTATGGATTCCTTTCAGAAAACAAAGAATTTGCCAGATGTTTGGCAGCAGAAGAT
GTCGTTTTCATTGGACCTGACACACATGCTATTCAAGCCATGGGCGACAAGATTGAAAGC
AAATTATTAGCTAAGAAAGCAGAGGTTAATACAATCCCTGGCTTTGATGGAGTAGTCAAG
GATGCAGAAGAAGCTGTCAGAATTGCGAGGGAAATTGGCTACCCTGTCATGATCAAGGCC
TCAGCAGGTGGTGGTGGGAAAGGCATGCGCATTGCTTGGGATGATGAAGAGACCAGGGAT
GGTTTTAGATTGTCATCTCAAGAAGCTGCTTCTAGTTTTGGCGATGATAGACTACTAATA
GAAAAATTTATTGATAATCCTCGTCATATAGAAATCCAGGTTCTAGGTGATAAACATGGG
AATGCTTTATGGCTTAATGAAAGAGAGTGCTCAATTCAGAGAAGAAATCAGAAGGTGGTG
GAGGAAGCACCAAGCATTTTTTTGGATGCGGAGACTCGAAGAGCGATGGGAGAACAAGCT
GTAGCTCTTGCCAGAGCAGTAAAATATTCCTCTGCTGGGACCGTGGAGTTCCTTGTGGAC
TCTAAGAAGAATTTTTATTTCTTGGAAATGAATACAAGACTCCAGGTTGAGCATCCTGTC
ACAGAATGCATTACTGGCCTGGACCTAGTCCAGGAAATGATCCGTGTTGCTAAGGGCTAC
CCTCTCAGGCACAAACAAGCTGATATTCGCATCAACGGCTGGGCAGTTGAATGTCGGGTT
TATGCTGAGGACCCCTACAAGTCTTTTGGTTTACCATCTATTGGGAGATTGTCTCAGTAC
CAAGAACCGTTACATCTACCTGGTGTCCGAGTGGACAGTGGCATCCAACCAGGAAGTGAT
ATTAGCATTTATTATGATCCTATGATTTCAAAACTAATCACATATGGCTCTGATAGAACT
GAGGCACTGAAGAGAATGGCAGATGCACTGGATAACTATGTTATTCGAGGTGTTACACAT
AATATTGCATTACTTCGAGAGGTGATAATCAACTCACGCTTTGTAAAAGGAGACATCAGC
ACTAAATTTCTCTCCGATGTGTATCCTGATGGCTTCAAAGGACACATGCTAACCAAGAGT
GAGAAGAACCAGTTATTGGCAATAGCATCATCATTGTTTGTGGCATTCCAGTTAAGAGCA
CAACATTTTCAAGAAAATTCAAGAATGCCTGTTATTAAACCAGACATAGCCAACTGGGAG
CTCTCAGTAAAATTGCATGATAAAGTTCATACCGTAGTAGCATCAAACAATGGGTCAGTG
TTCTCGGTGGAAGTTGATGGGTCGAAACTAAATGTGACCAGCACGTGGAACCTGGCTTCG
CCCTTATTGTCTGTCAGCGTTGATGGCACTCAGAGGACTGTCCAGTGTCTTTCTCGAGAA
GCAGGTGGAAACATGAGCATTCAGTTTCTTGGTACAGTGTACAAGGTGAATATCTTAACC
AGACTTGCCGCAGAATTGAACAAATTTATGCTGGAAAAAGTGACTGAGGACACAAGCAGT
GTTCTGCGTTCCCCGATGCCCGGAGTGGTGGTGGCCGTCTCTGTCAAGCCTGGAGACGCG
GTAGCAGAAGGTCAAGAAATTTGTGTGATTGAAGCCATGAAAATGCAGAATAGTATGACA
GCTGGGAAAACTGGCACGGTGAAATCTGTGCACTGTCAAGCTGGAGACACAGTTGGAGAA
GGGGATCTGCTCGTGGAGCTGGAA

ORF Protein

Cloned ORF protein sequence for pF1KE2601 Download

>KIEE2601  728 aa
MAGFWVGTAPLVAAGRRGRWPPQQLMLSAALRTLKHVLYYSRQCLMVSRNLGSVGYDPNE
KTFDKILVANRGEIACRVIRTCKKMGIKTVAIHSDVDASSVHVKMADEAVCVGPAPTSKS
YLNMDAIMEAIKKTRAQAVHPGYGFLSENKEFARCLAAEDVVFIGPDTHAIQAMGDKIES
KLLAKKAEVNTIPGFDGVVKDAEEAVRIAREIGYPVMIKASAGGGGKGMRIAWDDEETRD
GFRLSSQEAASSFGDDRLLIEKFIDNPRHIEIQVLGDKHGNALWLNERECSIQRRNQKVV
EEAPSIFLDAETRRAMGEQAVALARAVKYSSAGTVEFLVDSKKNFYFLEMNTRLQVEHPV
TECITGLDLVQEMIRVAKGYPLRHKQADIRINGWAVECRVYAEDPYKSFGLPSIGRLSQY
QEPLHLPGVRVDSGIQPGSDISIYYDPMISKLITYGSDRTEALKRMADALDNYVIRGVTH
NIALLREVIINSRFVKGDISTKFLSDVYPDGFKGHMLTKSEKNQLLAIASSLFVAFQLRA
QHFQENSRMPVIKPDIANWELSVKLHDKVHTVVASNNGSVFSVEVDGSKLNVTSTWNLAS
PLLSVSVDGTQRTVQCLSREAGGNMSIQFLGTVYKVNILTRLAAELNKFMLEKVTEDTSS
VLRSPMPGVVVAVSVKPGDAVAEGQEICVIEAMKMQNSMTAGKTGTVKSVHCQAGDTVGE
GDLLVELE

ORF+Vector

Nucleotide Sequence (with vector) for pF1KE2601 Download

>pF1KE2601  5297 bp
GGATCTCGATCCCGCGAAATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAA
TTCCCCACTAGTAATAATTTTCTTTAACTTTAGTAAGGAGCGATCGCCATGGCGGGGTTC
TGGGTCGGGACAGCACCGCTGGTCGCTGCCGGACGGCGTGGGCGGTGGCCGCCGCAGCAG
CTGATGCTGAGCGCGGCGCTGCGGACCCTGAAGCATGTTCTGTACTATTCAAGACAGTGC
TTAATGGTGTCCCGTAATCTTGGTTCAGTGGGATATGATCCTAATGAAAAAACTTTTGAT
AAAATTCTTGTTGCTAATAGAGGAGAAATTGCATGTCGGGTTATTAGAACTTGCAAGAAG
ATGGGCATTAAGACAGTTGCCATCCACAGTGATGTTGATGCTAGTTCTGTTCATGTGAAA
ATGGCGGATGAGGCTGTCTGTGTTGGCCCAGCTCCCACCAGTAAAAGCTACCTCAACATG
GATGCCATCATGGAAGCCATTAAGAAAACCAGGGCCCAAGCTGTACATCCAGGTTATGGA
TTCCTTTCAGAAAACAAAGAATTTGCCAGATGTTTGGCAGCAGAAGATGTCGTTTTCATT
GGACCTGACACACATGCTATTCAAGCCATGGGCGACAAGATTGAAAGCAAATTATTAGCT
AAGAAAGCAGAGGTTAATACAATCCCTGGCTTTGATGGAGTAGTCAAGGATGCAGAAGAA
GCTGTCAGAATTGCGAGGGAAATTGGCTACCCTGTCATGATCAAGGCCTCAGCAGGTGGT
GGTGGGAAAGGCATGCGCATTGCTTGGGATGATGAAGAGACCAGGGATGGTTTTAGATTG
TCATCTCAAGAAGCTGCTTCTAGTTTTGGCGATGATAGACTACTAATAGAAAAATTTATT
GATAATCCTCGTCATATAGAAATCCAGGTTCTAGGTGATAAACATGGGAATGCTTTATGG
CTTAATGAAAGAGAGTGCTCAATTCAGAGAAGAAATCAGAAGGTGGTGGAGGAAGCACCA
AGCATTTTTTTGGATGCGGAGACTCGAAGAGCGATGGGAGAACAAGCTGTAGCTCTTGCC
AGAGCAGTAAAATATTCCTCTGCTGGGACCGTGGAGTTCCTTGTGGACTCTAAGAAGAAT
TTTTATTTCTTGGAAATGAATACAAGACTCCAGGTTGAGCATCCTGTCACAGAATGCATT
ACTGGCCTGGACCTAGTCCAGGAAATGATCCGTGTTGCTAAGGGCTACCCTCTCAGGCAC
AAACAAGCTGATATTCGCATCAACGGCTGGGCAGTTGAATGTCGGGTTTATGCTGAGGAC
CCCTACAAGTCTTTTGGTTTACCATCTATTGGGAGATTGTCTCAGTACCAAGAACCGTTA
CATCTACCTGGTGTCCGAGTGGACAGTGGCATCCAACCAGGAAGTGATATTAGCATTTAT
TATGATCCTATGATTTCAAAACTAATCACATATGGCTCTGATAGAACTGAGGCACTGAAG
AGAATGGCAGATGCACTGGATAACTATGTTATTCGAGGTGTTACACATAATATTGCATTA
CTTCGAGAGGTGATAATCAACTCACGCTTTGTAAAAGGAGACATCAGCACTAAATTTCTC
TCCGATGTGTATCCTGATGGCTTCAAAGGACACATGCTAACCAAGAGTGAGAAGAACCAG
TTATTGGCAATAGCATCATCATTGTTTGTGGCATTCCAGTTAAGAGCACAACATTTTCAA
GAAAATTCAAGAATGCCTGTTATTAAACCAGACATAGCCAACTGGGAGCTCTCAGTAAAA
TTGCATGATAAAGTTCATACCGTAGTAGCATCAAACAATGGGTCAGTGTTCTCGGTGGAA
GTTGATGGGTCGAAACTAAATGTGACCAGCACGTGGAACCTGGCTTCGCCCTTATTGTCT
GTCAGCGTTGATGGCACTCAGAGGACTGTCCAGTGTCTTTCTCGAGAAGCAGGTGGAAAC
ATGAGCATTCAGTTTCTTGGTACAGTGTACAAGGTGAATATCTTAACCAGACTTGCCGCA
GAATTGAACAAATTTATGCTGGAAAAAGTGACTGAGGACACAAGCAGTGTTCTGCGTTCC
CCGATGCCCGGAGTGGTGGTGGCCGTCTCTGTCAAGCCTGGAGACGCGGTAGCAGAAGGT
CAAGAAATTTGTGTGATTGAAGCCATGAAAATGCAGAATAGTATGACAGCTGGGAAAACT
GGCACGGTGAAATCTGTGCACTGTCAAGCTGGAGACACAGTTGGAGAAGGGGATCTGCTC
GTGGAGCTGGAAGTTTAAACGAATTCGAGCTCGGTACCCGGGGATCCTCTAGAGTCGACC
TGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGC
TGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGG
TTTTTTGCTGAAAGGAGGAACTATATCCGGTTCGCTTGCTGTCCATAAAACCGCCCAGTC
TAGCTATCGCCATGTAAGCCCACTGCAAGCTACCTGCTTTCTCTTTGCGCTTGCGTTTTC
CCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACT
GGCTTTCTACGTGTTCCGCTTCCTTTAGCAGCCCTTGCGCCCTGAGTGCTTGCGGCAGCG
TGAGCTTCAAAAGAATTGCCAGCTGGGGCGCCCTCTGGTAAGGTTGGGAAGCCCTGCAAA
GTAAACTGGATGGCTTTCTTGCCGCCAAGGATCTGATGGCGCAGGGGATCAAGATCTGAT
CAAGAGACAGGATGACGGTCGTTTCGCATGCTTGAACAAGATGGATTGCACGCAGGTTCT
CCGGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAACAGACAATCGGCTGC
TCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACC
GACCTGTCCGGTGCCCTGAATGAACTGCAGGACGAGGCAGCGCGGCTATCGTGGCTGGCC
ACGACGGGCGTTCCTTGCGCAGCTGTGCTCGACGTTGTCACTGAAGCGGGAAGGGACTGG
CTGCTATTGGGCGAAGTGCCGGGGCAGGATCTCCTGTCATCTCACCTTGCTCCTGCCGAG
AAAGTATCCATCATGGCTGATGCAATGCGGCGGCTGCATACGCTTGATCCGGCTACCTGC
CCATTCGACCACCAAGCGAAACATCGCATCGAGCGAGCACGCACTCGGATGGAAGCCGGT
CTTGTCGATCAGGATGATCTGGACGAAGAGCATCAGGGGCTCGCGCCAGCCGAACTGTTC
GCCAGGCTCAAGGCGCGTATGCCGGATGGTGAGGATCTCGTCGTGACTCATGGCGATGCC
TGCTTGCCGAATATCATGGTGGAAAATGGCCGCTTTTCTGGATTCATCGACTGTGGCCGG
CTGGGTGTGGCGGACCGCTATCAGGACATAGCGTTGGCTACCCGTGATATTGCTGAAGAG
CTTGGCGGCGAATGGGCTGACCGCTTCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCG
CAGCGCATCGCCTTCTATCGCCTTCTTGACGAGTTCTTCTGAGCGGGACTCTGGGGTTCG
AAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTT
ATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGC
CAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGA
GCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATA
CCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTAC
CGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTG
TAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCC
CGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAG
ACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGT
AGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGT
ATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTG
ATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTAC
GCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCA
GTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCAC
CTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGAT
GGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACC
CTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTT
CGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAG
CGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCG
AGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCTTTTCAG
CCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGG
CAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGC
CGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAAC
GAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTC
TCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAG
GGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCC
TGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTTTGTTTATTTTTCTAAATACATTCA
AATATGTATCCGCTCAT