(Linear) (Six-base) MAPSORT of: KIEE2601.seq Check: 4071 from: 1 to: 2184 KIEE2601.orf With 182 enzymes: SgfI * January 19, 2016 12:01 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 655 1852 2184 Size: 655 1197 332 Fragments arranged by size: 1197 655 332 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 121 1408 2184 Size: 121 1287 776 Fragments arranged by size: 1287 776 121 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1999 2184 Size: 1999 185 AlwNI CAG_nnn'CTG Cuts at: 0 72 2184 Size: 72 2112 ApaI G_GGCC'C Cuts at: 0 408 2184 Size: 408 1776 ApaLI G'TGCA_C Cuts at: 0 2128 2184 Size: 2128 56 ApoI r'AATT_y Cuts at: 0 194 451 784 1029 1504 1635 1942 2057 Size: 194 257 333 245 475 131 307 115 Cuts at: 2057 2184 Size: 127 Fragments arranged by size: 475 333 307 257 245 194 131 127 115 AvaI C'yCGr_G Cuts at: 0 1853 2184 Size: 1853 331 BanII G_rGCy'C Cuts at: 0 408 1682 2184 Size: 408 1274 502 Fragments arranged by size: 1274 502 408 BbvCI CC'TCA_GC Cuts at: 0 660 1205 2184 Size: 660 545 979 Fragments arranged by size: 979 660 545 BciVI GTATCCnnnnn_n' Cuts at: 0 1532 2184 Size: 1532 652 BclI T'GATC_A Cuts at: 0 650 2184 Size: 650 1534 BfrBI ATG'CAT Cuts at: 0 1088 2184 Size: 1088 1096 BlpI GC'TnA_GC Cuts at: 0 79 2184 Size: 79 2105 Bme1580I G_kGCm'C Cuts at: 0 408 2132 2184 Size: 408 1724 52 Fragments arranged by size: 1724 408 52 BmrI ACTGGGnnnn_n' Cuts at: 0 1683 2184 Size: 1683 501 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1268 2184 Size: 1268 916 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1045 2051 2162 2184 Size: 1045 1006 111 22 Fragments arranged by size: 1045 1006 111 22 Bpu10I CC'TnA_GC Cuts at: 0 660 1130 1205 2184 Size: 660 470 75 979 Fragments arranged by size: 979 660 470 75 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 722 2184 Size: 722 1462 BsaI GGTCTCn'nnnn_ Cuts at: 0 703 2184 Size: 703 1481 BsaAI yAC'GTr Cuts at: 0 1784 2184 Size: 1784 400 BsaBI GATnn'nnATC Cuts at: 0 732 1520 2184 Size: 732 788 664 Fragments arranged by size: 788 732 664 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1994 2024 2054 2135 2165 2184 Size: 1994 30 30 81 30 19 Fragments arranged by size: 1994 81 30 30 30 19 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 227 2184 Size: 227 1957 BseYI C'CCAG_C Cuts at: 0 339 994 1178 2101 2184 Size: 339 655 184 923 83 Fragments arranged by size: 923 655 339 184 83 BsiHKAI G_wGCw'C Cuts at: 0 871 1620 1682 2132 2184 Size: 871 749 62 450 52 Fragments arranged by size: 871 749 450 62 52 BsmI GAATG_Cn' Cuts at: 0 846 1090 1603 1649 1877 2184 Size: 846 244 513 46 228 307 Fragments arranged by size: 846 513 307 244 228 46 BsmBI CGTCTCn'nnnn_ Cuts at: 0 2022 2027 2184 Size: 2022 5 157 Fragments arranged by size: 2022 157 5 Bsp1286I G_dGCh'C Cuts at: 0 408 871 1620 1682 2132 2184 Size: 408 463 749 62 450 52 Fragments arranged by size: 749 463 450 408 62 52 BspHI T'CATG_A Cuts at: 0 647 2184 Size: 647 1537 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 655 1852 2184 Size: 655 1197 332 Fragments arranged by size: 1197 655 332 BsrDI GCAATG_nn' Cuts at: 0 689 2184 Size: 689 1495 BsrGI T'GTAC_A Cuts at: 0 414 1898 2184 Size: 414 1484 286 Fragments arranged by size: 1484 414 286 BssSI C'ACGA_G Cuts at: 0 2170 2184 Size: 2170 14 BstAPI GCAn_nnn'nTGC Cuts at: 0 75 2184 Size: 75 2109 BstXI CCAn_nnnn'nTGG Cuts at: 0 465 2184 Size: 465 1719 BstYI r'GATC_y Cuts at: 0 2163 2184 Size: 2163 21 BtgI C'CryG_G Cuts at: 0 518 1001 2184 Size: 518 483 1183 Fragments arranged by size: 1183 518 483 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 776 958 2184 Size: 776 182 1226 Fragments arranged by size: 1226 776 182 BtsI GCAGTG_nn' Cuts at: 0 1983 2184 Size: 1983 201 EaeI y'GGCC_r Cuts at: 0 58 2012 2184 Size: 58 1954 172 Fragments arranged by size: 1954 172 58 EarI CTCTTCn'nnn_ Cuts at: 0 701 933 1384 2184 Size: 701 232 451 800 Fragments arranged by size: 800 701 451 232 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 331 2184 Size: 331 1853 EcoICRI GAG'CTC Cuts at: 0 1680 2184 Size: 1680 504 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 121 1045 1408 2051 2162 2184 Size: 121 924 363 643 111 22 Fragments arranged by size: 924 643 363 121 111 22 EcoNI CCTnn'n_nnAGG Cuts at: 0 1108 2184 Size: 1108 1076 EcoO109I rG'GnC_Cy Cuts at: 0 404 1209 2184 Size: 404 805 975 Fragments arranged by size: 975 805 404 FspI TGC'GCA Cuts at: 0 688 2184 Size: 688 1496 FspAI rTGC'GCAy Cuts at: 0 688 2184 Size: 688 1496 HaeII r_GCGC'y Cuts at: 0 91 2184 Size: 91 2093 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 870 902 2184 Size: 870 32 1282 Fragments arranged by size: 1282 870 32 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1329 2133 2184 Size: 1329 804 51 Fragments arranged by size: 1329 804 51 MslI CAynn'nnrTG Cuts at: 0 281 839 2080 2184 Size: 281 558 1241 104 Fragments arranged by size: 1241 558 281 104 MspA1I CmG'CkG Cuts at: 0 30 72 2101 2184 Size: 30 42 2029 83 Fragments arranged by size: 2029 83 42 30 NcoI C'CATG_G Cuts at: 0 518 2184 Size: 518 1666 NdeI CA'TA_TG Cuts at: 0 1362 2184 Size: 1362 822 NsiI A_TGCA'T Cuts at: 0 1090 2184 Size: 1090 1094 NspI r_CATG'y Cuts at: 0 109 227 508 687 1548 2184 Size: 109 118 281 179 861 636 Fragments arranged by size: 861 636 281 179 118 109 PflMI CCAn_nnn'nTGG Cuts at: 0 427 1240 1575 1784 2184 Size: 427 813 335 209 400 Fragments arranged by size: 813 427 400 335 209 PfoI T'CCnGG_A Cuts at: 0 1109 2184 Size: 1109 1075 PmlI CAC'GTG Cuts at: 0 1784 2184 Size: 1784 400 PpuMI rG'GwC_Cy Cuts at: 0 1209 2184 Size: 1209 975 PspOMI G'GGCC_C Cuts at: 0 404 2184 Size: 404 1780 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 138 170 2184 Size: 138 32 2014 Fragments arranged by size: 2014 138 32 PvuII CAG'CTG Cuts at: 0 72 2101 2184 Size: 72 2029 83 Fragments arranged by size: 2029 83 72 SacI G_AGCT'C Cuts at: 0 1682 2184 Size: 1682 502 SapI GCTCTTCn'nnn_ Cuts at: 0 933 2184 Size: 933 1251 SexAI A'CCwGG_T Cuts at: 0 1278 2184 Size: 1278 906 SfcI C'TryA_G Cuts at: 0 959 2184 Size: 959 1225 SmlI C'TyrA_G Cuts at: 0 737 1853 2184 Size: 737 1116 331 Fragments arranged by size: 1116 737 331 SphI G_CATG'C Cuts at: 0 687 2184 Size: 687 1497 SspI AAT'ATT Cuts at: 0 985 1443 2184 Size: 985 458 741 Fragments arranged by size: 985 741 458 StuI AGG'CCT Cuts at: 0 658 2184 Size: 658 1526 StyI C'CwwG_G Cuts at: 0 518 2184 Size: 518 1666 TatI w'GTAC_w Cuts at: 0 113 414 1898 2184 Size: 113 301 1484 286 Fragments arranged by size: 1484 301 286 113 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1994 2026 2184 Size: 1994 32 158 Fragments arranged by size: 1994 158 32 Tth111I GACn'n_nGTC Cuts at: 0 1105 2184 Size: 1105 1079 XhoI C'TCGA_G Cuts at: 0 1853 2184 Size: 1853 331 Enzymes that do cut: AarI AcuI AlfI AlwNI ApaI ApaLI ApoI AvaI BanII BbvCI BciVI BclI BfrBI BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaXI BseRI BseYI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrDI BsrGI BssSI BstAPI BstXI BstYI BtgI BtgZI BtsI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FspI FspAI HaeII Hin4I MmeI MslI MspA1I NcoI NdeI NsiI NspI PflMI PfoI PmlI PpuMI PspOMI PsrI PvuII SacI SapI SexAI SfcI SmlI SphI SspI StuI StyI TatI TstI Tth111I XhoI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AloI AscI AseI AsiSI AvrII BaeI BamHI BanI BbeI BbsI BcgI BglI BglII BmgBI BmtI BsaHI BsaWI BsgI BsiEI BsiWI BspEI BsrBI BsrFI BssHII BstBI BstEII BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI EagI EcoRI EcoRV FalI FseI HincII HindIII HpaI KasI KpnI MfeI MluI MscI NaeI NarI NgoMIV NheI NotI NruI PacI PasI PciI PmeI PpiI PshAI PsiI PspXI PstI PvuI RsrII SacII SalI SanDI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SwaI TaqII XbaI XcmI XmaI XmnI ZraI