P2RX5 ORF clone FHC10528

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC10528

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Clone info.

Species

human

Clone Name

pFN21AB4832 Sequence (with vector) Restriction Map

Product ID

FHC10528

Flexi Clone PID

FXC10528

Original Clone PID

ORK10528

ORF Sequence

DNA sequence (1266 bp)
Protein sequence (422 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB528606]

Gene

KIBB4832

Symbol

P2RX5

Alias : LRH-1, P2X5, P2X5R

Description

purinergic receptor P2X, ligand gated ion channel, 5, transcript variant 1

Original Clone ID

bm03021

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1266 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 422 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS11034.1 3.4e-184 100.0 P2RX5 CCDS56015.1 9.8e-183 99.5 P2RX5 CCDS56014.1 6.5e-133 94.3 P2RX5 CCDS11035.1 1.9e-131 93.8 P2RX5 CCDS9214.1 2.7e-73 50.3 P2RX4 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR003048 1 13 PR01312 P2X5 purinoceptor IPR003048 58 68 PR01312 P2X5 purinoceptor IPR001429 84 92 PR01307 P2X purinoreceptor IPR003048 119 128 PR01312 P2X5 purinoceptor IPR001429 161 172 PR01307 P2X purinoreceptor IPR001429 245 257 PR01307 P2X purinoreceptor IPR001429 291 301 PR01307 P2X purinoreceptor IPR001429 311 325 PR01307 P2X purinoreceptor IPR003048 339 347 PR01312 P2X5 purinoceptor HMMPfam IPR001429 14 330 PF00864 P2X purinoreceptor IPR001429 328 364 PF00864 P2X purinoreceptor HMMTigr IPR001429 1 351 TIGR00863 P2X purinoreceptor
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 30 GLLYRLLQASILAYLVVWVFLI 51 PRIMARY 22
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_002552 422 602836 100.0 100.0 NP_001191448 421 602836 99.5 100.0 NP_001191449 398 602836 94.3 100.0 NP_778255 397 602836 93.8 100.0 NP_002551 388 600846 50.3 86.3 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB4832 Download

>KIBB4832  1266 bp
ATGGGGCAGGCGGGCTGCAAGGGGCTCTGCCTGTCGCTGTTCGACTACAAGACCGAGAAG
TATGTCATCGCCAAGAACAAGAAGGTGGGCCTGCTGTACCGGCTGCTGCAGGCCTCCATC
CTGGCGTACCTGGTCGTATGGGTGTTCCTGATAAAGAAGGGTTACCAAGACGTCGACACC
TCCCTGCAGAGTGCTGTCATCACCAAAGTCAAGGGCGTGGCCTTCACCAACACCTCGGAT
CTTGGGCAGCGGATCTGGGATGTCGCCGACTACGTCATTCCAGCCCAGGGAGAGAACGTC
TTTTTTGTGGTCACCAACCTGATTGTGACCCCCAACCAGCGGCAGAACGTCTGTGCTGAG
AATGAAGGCATTCCTGATGGCGCGTGCTCCAAGGACAGCGACTGCCACGCTGGGGAAGCG
GTTACAGCTGGAAACGGAGTGAAGACCGGCCGCTGCCTGCGGAGAGAGAACTTGGCCAGG
GGCACCTGTGAGATCTTTGCCTGGTGCCCGTTGGAGACAAGCTCCAGGCCGGAGGAGCCA
TTCCTGAAGGAGGCCGAAGACTTCACCATTTTCATAAAGAACCACATCCGTTTCCCCAAA
TTCAACTTCTCCAAAAGCAATGTGATGGACGTCAAGGACAGATCTTTCCTGAAATCATGC
CACTTTGGCCCCAAGAACCACTACTGCCCCATCTTCCGACTGGGCTCCGTGATCCGCTGG
GCCGGGAGCGACTTCCAGGATATAGCCCTGGAGGGTGGCGTGATAGGAATTAATATTGAA
TGGAACTGTGATCTTGATAAAGCTGCCTCTGAGTGCCACCCTCACTATTCTTTTAGCCGT
CTGGACAATAAACTTTCAAAGTCTGTCTCCTCCGGGTACAACTTCAGATTTGCCAGATAT
TACCGAGACGCAGCCGGGGTGGAGTTCCGCACCCTGATGAAAGCCTACGGGATCCGCTTT
GACGTGATGGTGAACGGCAAGGGTGCTTTCTTCTGCGACCTGGTACTCATCTACCTCATC
AAAAAGAGAGAGTTTTACCGTGACAAGAAGTACGAGGAAGTGAGGGGCCTAGAAGACAGT
TCCCAGGAGGCCGAGGACGAGGCATCGGGGCTGGGGCTATCTGAGCAGCTCACATCTGGG
CCAGGGCTGCTGGGGATGCCGGAGCAGCAGGAGCTGCAGGAGCCACCCGAGGCGAAGCGT
GGAAGCAGCAGTCAGAAGGGGAACGGATCTGTGTGCCCACAGCTCCTGGAGCCCCACAGG
AGCACG

ORF Protein

Cloned ORF protein sequence for pFN21AB4832 Download

>KIBB4832  422 aa
MGQAGCKGLCLSLFDYKTEKYVIAKNKKVGLLYRLLQASILAYLVVWVFLIKKGYQDVDT
SLQSAVITKVKGVAFTNTSDLGQRIWDVADYVIPAQGENVFFVVTNLIVTPNQRQNVCAE
NEGIPDGACSKDSDCHAGEAVTAGNGVKTGRCLRRENLARGTCEIFAWCPLETSSRPEEP
FLKEAEDFTIFIKNHIRFPKFNFSKSNVMDVKDRSFLKSCHFGPKNHYCPIFRLGSVIRW
AGSDFQDIALEGGVIGINIEWNCDLDKAASECHPHYSFSRLDNKLSKSVSSGYNFRFARY
YRDAAGVEFRTLMKAYGIRFDVMVNGKGAFFCDLVLIYLIKKREFYRDKKYEEVRGLEDS
SQEAEDEASGLGLSEQLTSGPGLLGMPEQQELQEPPEAKRGSSSQKGNGSVCPQLLEPHR
ST

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB4832 Download

>pFN21AB4832  5971 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGGGCAGGCGGGCTGCAAGGGGCTCTGCCTGTC
GCTGTTCGACTACAAGACCGAGAAGTATGTCATCGCCAAGAACAAGAAGGTGGGCCTGCT
GTACCGGCTGCTGCAGGCCTCCATCCTGGCGTACCTGGTCGTATGGGTGTTCCTGATAAA
GAAGGGTTACCAAGACGTCGACACCTCCCTGCAGAGTGCTGTCATCACCAAAGTCAAGGG
CGTGGCCTTCACCAACACCTCGGATCTTGGGCAGCGGATCTGGGATGTCGCCGACTACGT
CATTCCAGCCCAGGGAGAGAACGTCTTTTTTGTGGTCACCAACCTGATTGTGACCCCCAA
CCAGCGGCAGAACGTCTGTGCTGAGAATGAAGGCATTCCTGATGGCGCGTGCTCCAAGGA
CAGCGACTGCCACGCTGGGGAAGCGGTTACAGCTGGAAACGGAGTGAAGACCGGCCGCTG
CCTGCGGAGAGAGAACTTGGCCAGGGGCACCTGTGAGATCTTTGCCTGGTGCCCGTTGGA
GACAAGCTCCAGGCCGGAGGAGCCATTCCTGAAGGAGGCCGAAGACTTCACCATTTTCAT
AAAGAACCACATCCGTTTCCCCAAATTCAACTTCTCCAAAAGCAATGTGATGGACGTCAA
GGACAGATCTTTCCTGAAATCATGCCACTTTGGCCCCAAGAACCACTACTGCCCCATCTT
CCGACTGGGCTCCGTGATCCGCTGGGCCGGGAGCGACTTCCAGGATATAGCCCTGGAGGG
TGGCGTGATAGGAATTAATATTGAATGGAACTGTGATCTTGATAAAGCTGCCTCTGAGTG
CCACCCTCACTATTCTTTTAGCCGTCTGGACAATAAACTTTCAAAGTCTGTCTCCTCCGG
GTACAACTTCAGATTTGCCAGATATTACCGAGACGCAGCCGGGGTGGAGTTCCGCACCCT
GATGAAAGCCTACGGGATCCGCTTTGACGTGATGGTGAACGGCAAGGGTGCTTTCTTCTG
CGACCTGGTACTCATCTACCTCATCAAAAAGAGAGAGTTTTACCGTGACAAGAAGTACGA
GGAAGTGAGGGGCCTAGAAGACAGTTCCCAGGAGGCCGAGGACGAGGCATCGGGGCTGGG
GCTATCTGAGCAGCTCACATCTGGGCCAGGGCTGCTGGGGATGCCGGAGCAGCAGGAGCT
GCAGGAGCCACCCGAGGCGAAGCGTGGAAGCAGCAGTCAGAAGGGGAACGGATCTGTGTG
CCCACAGCTCCTGGAGCCCCACAGGAGCACGGTTTAAACGAATTCGGGCTCGGTACCCGG
GGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAA
AGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGG
CCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATG
CAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATT
ATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAG
GGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAA
TTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACA
TTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACG
TCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATA
CATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGA
AAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCA
TTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGAT
CAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAG
AGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGC
GCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCT
CAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACA
GTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTT
CTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCAT
GTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGT
GACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTA
CTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGA
CCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGT
GAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATC
GTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCT
GAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCC
AACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACG
CAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGT
TGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAA
GTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCT
CCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCC
CTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGG
TCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCT
TATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAG
CAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGA
AGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGA
AGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTG
GTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAG
AAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAG
GGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAA
ATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAG
CTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATA
CGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTA
CCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTA
TGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCC
ACGATGCGTCCGGCGTAGAGGATCGAGATCT