(Circular) (Six-base) MAPSORT of: pFN21AB4832.seq Check: 5977 from: 1 to: 5971 pFN21AB4832 5971 bp With 182 enzymes: SgfI * October 20, 2009 10:47 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2178 2637 3781 278 Size: 53 83 186 936 642 459 1144 2468 Fragments arranged by size: 2468 1144 936 642 459 186 83 53 AccI GT'mk_AC Cuts at: 1937 2178 3314 1937 Size: 241 1136 4594 Fragments arranged by size: 4594 1136 241 Acc65I G'GTAC_C Cuts at: 3292 3292 Size: 5971 AclI AA'CG_TT Cuts at: 1563 4098 4471 1563 Size: 2535 373 3063 Fragments arranged by size: 3063 2535 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2570 2872 4034 5418 5686 1632 Size: 938 302 1162 1384 268 1917 Fragments arranged by size: 1917 1384 1162 938 302 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5774 AflIII A'CryG_T Cuts at: 1203 4870 5847 1203 Size: 3667 977 1327 Fragments arranged by size: 3667 1327 977 AgeI A'CCGG_T Cuts at: 4802 4802 Size: 5971 AhdI GACnn_n'nnGTC Cuts at: 4700 4700 Size: 5971 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5971 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5971 AlwNI CAG_nnn'CTG Cuts at: 2406 3178 3250 3714 5286 2406 Size: 772 72 464 1572 3091 Fragments arranged by size: 3091 1572 772 464 72 ApaLI G'TGCA_C Cuts at: 4028 5184 4028 Size: 1156 4815 ApoI r'AATT_y Cuts at: 1493 2603 3280 3506 3658 5767 1493 Size: 1110 677 226 152 2109 1697 Fragments arranged by size: 2109 1697 1110 677 226 152 AseI AT'TA_AT Cuts at: 160 1032 2775 4525 160 Size: 872 1743 1750 1606 Fragments arranged by size: 1750 1743 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5971 AvaI C'yCGr_G Cuts at: 1870 1943 3191 3296 1870 Size: 73 1248 105 4545 Fragments arranged by size: 4545 1248 105 73 BamHI G'GATC_C Cuts at: 2955 3301 3668 2955 Size: 346 367 5258 Fragments arranged by size: 5258 367 346 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2486 2508 3292 4747 618 Size: 325 217 627 699 22 784 1455 1842 Fragments arranged by size: 1842 1455 784 699 627 325 217 22 BanII G_rGCy'C Cuts at: 1391 2031 2711 3258 3290 1391 Size: 640 680 547 32 4072 Fragments arranged by size: 4072 680 640 547 32 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2453 2568 3084 928 Size: 971 554 115 516 3815 Fragments arranged by size: 3815 971 554 516 115 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4162 4196 1903 Size: 34 2225 34 3678 Fragments arranged by size: 3678 2225 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3863 5073 3863 Size: 1210 4761 BclI T'GATC_A Cuts at: 1548 1548 Size: 5971 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2670 4582 136 Size: 107 122 71 631 605 998 1912 1525 Fragments arranged by size: 1912 1525 998 631 605 122 107 71 BglII A'GATC_T Cuts at: 2496 2645 5966 2496 Size: 149 3321 2501 Fragments arranged by size: 3321 2501 149 BlpI GC'TnA_GC Cuts at: 3388 3388 Size: 5971 Bme1580I G_kGCm'C Cuts at: 1790 2489 2513 3242 4032 5188 1790 Size: 699 24 729 790 1156 2573 Fragments arranged by size: 2573 1156 790 729 699 24 BmgBI CAC'GTC Cuts at: 1318 2968 1318 Size: 1650 4321 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2714 3702 4660 Size: 416 53 83 399 24 1297 988 958 Cuts at: 4660 442 Size: 1753 Fragments arranged by size: 1753 1297 988 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5971 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2542 2542 Size: 5971 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2512 2774 3272 4631 1239 Size: 1273 262 498 1359 2579 Fragments arranged by size: 2579 1359 1273 498 262 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4096 4961 5259 4096 Size: 865 298 4808 Fragments arranged by size: 4808 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4634 882 Size: 617 3135 2219 Fragments arranged by size: 3135 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3762 493 Size: 711 2558 2702 Fragments arranged by size: 2702 2558 711 BsaBI GATnn'nnATC Cuts at: 5965 5965 Size: 5971 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2175 2634 3778 4160 Size: 53 83 186 936 642 459 1144 382 Cuts at: 4160 4795 275 Size: 635 1451 Fragments arranged by size: 1451 1144 936 642 635 459 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4404 4802 5076 5223 5634 4404 Size: 398 274 147 411 4741 Fragments arranged by size: 4741 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2286 2316 1178 Size: 30 165 30 883 30 4833 Fragments arranged by size: 4833 883 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2552 2863 1186 Size: 1366 311 4294 Fragments arranged by size: 4294 1366 311 BseYI C'CCAG_C Cuts at: 2414 2721 3115 3154 5174 5670 2414 Size: 307 394 39 2020 496 2715 Fragments arranged by size: 2715 2020 496 394 307 39 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5398 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2455 3421 4182 5210 1540 Size: 275 186 454 966 761 1028 2301 Fragments arranged by size: 2301 1028 966 761 454 275 186 BsiHKAI G_wGCw'C Cuts at: 2393 3269 4032 4117 5188 2393 Size: 876 763 85 1071 3176 Fragments arranged by size: 3176 1071 876 763 85 BsmI GAATG_Cn' Cuts at: 2373 3482 3575 2373 Size: 1109 93 4769 Fragments arranged by size: 4769 1109 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2904 2904 Size: 5971 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2031 2393 2489 2513 2711 3242 3258 Size: 399 241 362 96 24 198 531 16 Cuts at: 3258 3269 3290 4032 4117 5188 1391 Size: 11 21 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 531 399 362 241 198 96 85 24 21 16 11 BspEI T'CCGG_A Cuts at: 5634 5634 Size: 5971 BspHI T'CATG_A Cuts at: 3860 5590 3860 Size: 1730 4241 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3326 844 Size: 2482 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3858 1394 Size: 429 2035 3507 Fragments arranged by size: 3507 2035 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2629 4466 4640 65 Size: 1183 1381 1837 174 1396 Fragments arranged by size: 1837 1396 1381 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2103 2450 4615 4802 1700 Size: 110 293 347 2165 187 2869 Fragments arranged by size: 2869 2165 347 293 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5971 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5971 BssSI C'ACGA_G Cuts at: 4031 5043 4031 Size: 1012 4959 BstAPI GCAn_nnn'nTGC Cuts at: 794 3178 794 Size: 2384 3587 BstBI TT'CG_AA Cuts at: 4775 4775 Size: 5971 BstEII G'GTnAC_C Cuts at: 1184 2165 2314 1184 Size: 981 149 4841 Fragments arranged by size: 4841 981 149 BstYI r'GATC_y Cuts at: 1972 2242 2256 2496 2645 2955 3230 3301 3668 Size: 270 14 240 149 310 275 71 367 Cuts at: 3668 4053 4070 5522 5608 5620 5966 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 385 367 346 310 275 270 240 149 86 71 17 14 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4480 Fragments arranged by size: 4480 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2056 487 Size: 683 56 830 4402 Fragments arranged by size: 4402 830 683 56 BtsI GCAGTG_nn' Cuts at: 3487 4281 4301 3487 Size: 794 20 5157 Fragments arranged by size: 5157 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5971 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5936 DraI TTT'AAA Cuts at: 3275 3275 Size: 5971 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4978 809 Size: 507 3662 1802 Fragments arranged by size: 3662 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2452 2478 3418 Size: 54 1425 50 275 18 622 26 940 Cuts at: 3418 4307 5936 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 940 889 622 275 54 50 43 26 18 EagI C'GGCC_G Cuts at: 1537 1812 2452 3418 1537 Size: 275 640 966 4090 Fragments arranged by size: 4090 966 640 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3901 1353 Size: 2548 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4558 4932 5078 387 Size: 1064 138 2969 374 146 1280 Fragments arranged by size: 2969 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2512 2570 2774 2872 3272 4034 4631 Size: 393 880 58 204 98 400 762 597 Cuts at: 4631 5418 5686 1239 Size: 787 268 1524 Fragments arranged by size: 1524 880 787 762 597 400 393 268 204 98 58 EcoNI CCTnn'n_nnAGG Cuts at: 2552 2552 Size: 5971 EcoO109I rG'GnC_Cy Cuts at: 3070 3070 Size: 5971 EcoRI G'AATT_C Cuts at: 3280 3658 3280 Size: 378 5593 FspI TGC'GCA Cuts at: 4477 4477 Size: 5971 HaeII r_GCGC'y Cuts at: 1678 5118 1678 Size: 3440 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2286 2318 3092 3124 4612 Size: 32 135 32 714 32 774 32 1488 Cuts at: 4612 4644 4686 4718 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1488 774 714 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2179 3315 3561 669 Size: 988 281 241 1136 246 3079 Fragments arranged by size: 3079 1136 988 281 246 241 HindIII A'AGCT_T Cuts at: 748 748 Size: 5971 HpaI GTT'AAC Cuts at: 3561 3561 Size: 5971 KpnI G_GTAC'C Cuts at: 3296 3296 Size: 5971 MfeI C'AATT_G Cuts at: 3570 3570 Size: 5971 MluI A'CGCG_T Cuts at: 5847 5847 Size: 5971 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2496 2725 5085 5269 1305 Size: 1191 229 2360 184 2007 Fragments arranged by size: 2360 2007 1191 229 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2480 10 Size: 54 1425 343 648 3501 Fragments arranged by size: 3501 1425 648 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2939 3929 4288 4447 518 Size: 648 1773 990 359 159 2042 Fragments arranged by size: 2042 1773 990 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2254 2344 2432 2457 2721 3387 4064 5212 Size: 648 90 88 25 264 666 677 1148 Cuts at: 5212 5457 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 666 648 264 245 90 88 25 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4480 Fragments arranged by size: 4480 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5971 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5971 NotI GC'GGCC_GC Cuts at: 3418 3418 Size: 5971 NspI r_CATG'y Cuts at: 1132 1229 3329 4874 1132 Size: 97 2100 1545 2229 Fragments arranged by size: 2229 2100 1545 97 PasI CC'CwG_GG Cuts at: 1348 2290 1348 Size: 942 5029 PciI A'CATG_T Cuts at: 4870 4870 Size: 5971 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5971 PfoI T'CCnGG_A Cuts at: 2739 3249 2739 Size: 510 5461 PmeI GTTT'AAAC Cuts at: 3275 3275 Size: 5971 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4040 4072 5553 5585 4040 Size: 32 1481 32 4426 Fragments arranged by size: 4426 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5971 PsiI TTA'TAA Cuts at: 3541 3541 Size: 5971 PstI C_TGCA'G Cuts at: 830 2115 2193 3183 3323 830 Size: 1285 78 990 140 3478 Fragments arranged by size: 3478 1285 990 140 78 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5971 PvuII CAG'CTG Cuts at: 2432 2432 Size: 5971 SalI G'TCGA_C Cuts at: 1936 2177 3313 1936 Size: 241 1136 4594 Fragments arranged by size: 4594 1136 241 SbfI CC_TGCA'GG Cuts at: 3323 3323 Size: 5971 ScaI AGT'ACT Cuts at: 4219 5931 4219 Size: 1712 4259 SexAI A'CCwGG_T Cuts at: 2133 3003 2133 Size: 870 5101 SfcI C'TryA_G Cuts at: 826 1045 2111 2189 3179 3319 4454 5135 5326 Size: 219 1066 78 990 140 1135 681 191 Cuts at: 5326 826 Size: 1471 Fragments arranged by size: 1471 1135 1066 990 681 219 191 140 78 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5971 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5971 SmaI CCC'GGG Cuts at: 1872 3298 1872 Size: 1426 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4075 4976 5238 820 Size: 197 926 2132 901 262 1553 Fragments arranged by size: 2132 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3762 493 Size: 3269 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5971 SphI G_CATG'C Cuts at: 1132 3329 1132 Size: 2197 3774 SspI AAT'ATT Cuts at: 5 52 2779 3895 5 Size: 47 2727 1116 2081 Fragments arranged by size: 2727 2081 1116 47 StuI AGG'CCT Cuts at: 1517 2117 1517 Size: 600 5371 StyI C'CwwG_G Cuts at: 513 1065 2004 2394 3410 513 Size: 552 939 390 1016 3074 Fragments arranged by size: 3074 1016 939 552 390 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2072 2134 3998 4015 4168 4353 4798 Size: 1255 492 62 1864 17 153 185 445 Cuts at: 4798 325 Size: 1498 Fragments arranged by size: 1864 1498 1255 492 445 185 153 62 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4217 5929 96 Size: 274 80 33 51 1443 2240 1712 138 Fragments arranged by size: 2240 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2427 2459 5931 5963 2427 Size: 32 3472 32 2435 Fragments arranged by size: 3472 2435 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2276 1316 Size: 960 5011 XbaI T'CTAG_A Cuts at: 3307 3307 Size: 5971 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2701 2701 Size: 5971 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5971 XmaI C'CCGG_G Cuts at: 1870 3296 1870 Size: 1426 4545 XmnI GAAnn'nnTTC Cuts at: 2373 4100 2373 Size: 1727 4244 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2176 2635 3779 276 Size: 53 83 186 936 642 459 1144 2468 Fragments arranged by size: 2468 1144 936 642 459 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI