SPNS1 ORF clone FHC08366

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC08366

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Clone info.

Species

human

Clone Name

pFN21AB0485 Sequence (with vector) Restriction Map

Product ID

FHC08366

Flexi Clone PID

FXC08366

ORF Sequence

DNA sequence (1584 bp)
Protein sequence (528 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB528362]

Gene

KIBB0485

Symbol

SPNS1

Alias : HSpin1, LAT, PP2030, SLC62A1, SLC63A1, SPIN1, SPINL, nrs

Description

spinster homolog 1 (Drosophila), transcript variant 1

Original Clone ID

cs01985

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1584 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 528 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS10646.1 2e-190 100.0 SPNS1 CCDS45454.1 2e-162 100.0 SPNS1 CCDS45452.1 1.6e-96 90.2 SPNS1 CCDS42237.1 8.6e-89 54.0 SPNS2 CCDS11045.1 8.5e-87 54.0 SPNS3 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR011701 68 416 PF07690 Major facilitator superfamily ProfileScan IPR020846 61 489 PS50850 Major facilitator superfamily domain
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 53 LSPGRSALIVAVLCYINLLNYMD 75 PRIMARY 23 2 97 SGLIQTVFISSYMVLAPVFGYLG 119 PRIMARY 23 3 126 YLMCGGIAFWSLVTLGSSFIPG 147 SECONDARY 22 4 149 HFWLLLLTRGLVGVGEASYSTIA 171 SECONDARY 23 5 186 RMLSIFYFAIPVGSGLGYIAGSK 208 SECONDARY 23 6 219 ALRVTPGLGVVAVLLLFLVVREP 241 PRIMARY 23 7 274 VLSSLGFTAVAFVTGSLALWAPA 296 SECONDARY 23 8 320 SDSLIFGLITCLTGVLGVGLGVE 342 SECONDARY 23 9 353 RADPLVCATGLLGSAPFLFLSLA 375 SECONDARY 23 10 381 IVATYIFIFIGETLLSMNWAIVA 403 PRIMARY 23 11 463 SLMLCAFVGALGGAAFLGTAIFI 485 PRIMARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_114427 528 612583 100.0 100.0 NP_001135920 528 612583 100.0 100.0 XP_016879248 573 612583 100.0 100.0 XP_016879247 578 612583 99.1 100.0 NP_001135922 455 612583 100.0 86.2 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB0485 Download

>KIBB0485  1584 bp
ATGGCCGGGTCCGACACCGCGCCCTTCCTCAGCCAGGCGGATGACCCGGACGACGGGCCA
GTGCCTGGCACCCCGGGGTTGCCAGGGTCCACGGGGAACCCGAAGTCCGAGGAGCCCGAG
GTCCCGGACCAGGAGGGGCTGCAGCGCATCACCGGCCTGTCTCCCGGCCGTTCGGCTCTC
ATAGTGGCGGTGCTGTGCTACATCAATCTCCTGAACTACATGGACCGCTTCACCGTGGCT
GGCGTCCTTCCCGACATCGAGCAGTTCTTCAACATCGGGGACAGTAGCTCTGGGCTCATC
CAGACCGTGTTCATCTCCAGTTACATGGTGTTGGCACCTGTGTTTGGCTACCTGGGTGAC
AGGTACAATCGGAAGTATCTCATGTGCGGGGGCATTGCCTTCTGGTCCCTGGTGACACTG
GGGTCATCCTTCATCCCCGGAGAGCATTTCTGGCTGCTCCTCCTGACCCGGGGCCTGGTG
GGGGTCGGGGAGGCCAGTTATTCCACCATCGCGCCCACTCTCATTGCCGACCTCTTTGTG
GCCGACCAGCGGAGCCGGATGCTCAGCATCTTCTACTTTGCCATTCCGGTGGGCAGTGGT
CTGGGCTACATTGCAGGCTCCAAAGTGAAGGATATGGCTGGAGACTGGCACTGGGCTCTG
AGGGTGACACCGGGTCTAGGAGTGGTGGCCGTTCTGCTGCTGTTCCTGGTAGTGCGGGAG
CCGCCAAGGGGAGCCGTGGAGCGCCACTCAGATTTGCCACCCCTGAACCCCACCTCGTGG
TGGGCAGATCTGAGGGCTCTGGCAAGAAATCCTAGTTTCGTCCTGTCTTCCCTGGGCTTC
ACTGCTGTGGCCTTTGTCACGGGCTCCCTGGCTCTGTGGGCTCCGGCATTCCTGCTGCGT
TCCCGCGTGGTCCTTGGGGAGACCCCACCCTGCCTTCCCGGAGACTCCTGCTCTTCCTCT
GACAGTCTCATCTTTGGACTCATCACCTGCCTGACCGGAGTCCTGGGTGTGGGCCTGGGT
GTGGAGATCAGCCGCCGGCTCCGCCACTCCAACCCCCGGGCTGATCCCCTGGTCTGTGCC
ACTGGCCTCCTGGGCTCTGCACCCTTCCTCTTCCTGTCCCTTGCCTGCGCCCGTGGTAGC
ATCGTGGCCACTTATATTTTCATCTTCATTGGAGAGACCCTCCTGTCCATGAACTGGGCC
ATCGTGGCCGACATTCTGCTGTACGTGGTGATCCCTACCCGACGCTCCACCGCCGAGGCC
TTCCAGATCGTGCTGTCCCACCTGCTGGGTGATGCTGGGAGCCCCTACCTCATTGGCCTG
ATCTCTGACCGCCTGCGCCGGAACTGGCCCCCCTCCTTCTTGTCCGAGTTCCGGGCTCTG
CAGTTCTCGCTCATGCTCTGCGCGTTTGTTGGGGCACTGGGCGGCGCAGCCTTCCTGGGC
ACCGCCATCTTCATTGAGGCCGACCGCCGGCGGGCACAGCTGCACGTGCAGGGCCTGCTG
CACGAAGCAGGGTCCACAGACGACCGGATTGTGGTGCCCCAGCGGGGCCGCTCCACCCGC
GTGCCCGTGGCCAGTGTGCTCATC

ORF Protein

Cloned ORF protein sequence for pFN21AB0485 Download

>KIBB0485  528 aa
MAGSDTAPFLSQADDPDDGPVPGTPGLPGSTGNPKSEEPEVPDQEGLQRITGLSPGRSAL
IVAVLCYINLLNYMDRFTVAGVLPDIEQFFNIGDSSSGLIQTVFISSYMVLAPVFGYLGD
RYNRKYLMCGGIAFWSLVTLGSSFIPGEHFWLLLLTRGLVGVGEASYSTIAPTLIADLFV
ADQRSRMLSIFYFAIPVGSGLGYIAGSKVKDMAGDWHWALRVTPGLGVVAVLLLFLVVRE
PPRGAVERHSDLPPLNPTSWWADLRALARNPSFVLSSLGFTAVAFVTGSLALWAPAFLLR
SRVVLGETPPCLPGDSCSSSDSLIFGLITCLTGVLGVGLGVEISRRLRHSNPRADPLVCA
TGLLGSAPFLFLSLACARGSIVATYIFIFIGETLLSMNWAIVADILLYVVIPTRRSTAEA
FQIVLSHLLGDAGSPYLIGLISDRLRRNWPPSFLSEFRALQFSLMLCAFVGALGGAAFLG
TAIFIEADRRRAQLHVQGLLHEAGSTDDRIVVPQRGRSTRVPVASVLI

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB0485 Download

>pFN21AB0485  6289 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCCGGGTCCGACACCGCGCCCTTCCTCAGCCA
GGCGGATGACCCGGACGACGGGCCAGTGCCTGGCACCCCGGGGTTGCCAGGGTCCACGGG
GAACCCGAAGTCCGAGGAGCCCGAGGTCCCGGACCAGGAGGGGCTGCAGCGCATCACCGG
CCTGTCTCCCGGCCGTTCGGCTCTCATAGTGGCGGTGCTGTGCTACATCAATCTCCTGAA
CTACATGGACCGCTTCACCGTGGCTGGCGTCCTTCCCGACATCGAGCAGTTCTTCAACAT
CGGGGACAGTAGCTCTGGGCTCATCCAGACCGTGTTCATCTCCAGTTACATGGTGTTGGC
ACCTGTGTTTGGCTACCTGGGTGACAGGTACAATCGGAAGTATCTCATGTGCGGGGGCAT
TGCCTTCTGGTCCCTGGTGACACTGGGGTCATCCTTCATCCCCGGAGAGCATTTCTGGCT
GCTCCTCCTGACCCGGGGCCTGGTGGGGGTCGGGGAGGCCAGTTATTCCACCATCGCGCC
CACTCTCATTGCCGACCTCTTTGTGGCCGACCAGCGGAGCCGGATGCTCAGCATCTTCTA
CTTTGCCATTCCGGTGGGCAGTGGTCTGGGCTACATTGCAGGCTCCAAAGTGAAGGATAT
GGCTGGAGACTGGCACTGGGCTCTGAGGGTGACACCGGGTCTAGGAGTGGTGGCCGTTCT
GCTGCTGTTCCTGGTAGTGCGGGAGCCGCCAAGGGGAGCCGTGGAGCGCCACTCAGATTT
GCCACCCCTGAACCCCACCTCGTGGTGGGCAGATCTGAGGGCTCTGGCAAGAAATCCTAG
TTTCGTCCTGTCTTCCCTGGGCTTCACTGCTGTGGCCTTTGTCACGGGCTCCCTGGCTCT
GTGGGCTCCGGCATTCCTGCTGCGTTCCCGCGTGGTCCTTGGGGAGACCCCACCCTGCCT
TCCCGGAGACTCCTGCTCTTCCTCTGACAGTCTCATCTTTGGACTCATCACCTGCCTGAC
CGGAGTCCTGGGTGTGGGCCTGGGTGTGGAGATCAGCCGCCGGCTCCGCCACTCCAACCC
CCGGGCTGATCCCCTGGTCTGTGCCACTGGCCTCCTGGGCTCTGCACCCTTCCTCTTCCT
GTCCCTTGCCTGCGCCCGTGGTAGCATCGTGGCCACTTATATTTTCATCTTCATTGGAGA
GACCCTCCTGTCCATGAACTGGGCCATCGTGGCCGACATTCTGCTGTACGTGGTGATCCC
TACCCGACGCTCCACCGCCGAGGCCTTCCAGATCGTGCTGTCCCACCTGCTGGGTGATGC
TGGGAGCCCCTACCTCATTGGCCTGATCTCTGACCGCCTGCGCCGGAACTGGCCCCCCTC
CTTCTTGTCCGAGTTCCGGGCTCTGCAGTTCTCGCTCATGCTCTGCGCGTTTGTTGGGGC
ACTGGGCGGCGCAGCCTTCCTGGGCACCGCCATCTTCATTGAGGCCGACCGCCGGCGGGC
ACAGCTGCACGTGCAGGGCCTGCTGCACGAAGCAGGGTCCACAGACGACCGGATTGTGGT
GCCCCAGCGGGGCCGCTCCACCCGCGTGCCCGTGGCCAGTGTGCTCATCGTTTAAACGAA
TTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGG
CTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAG
CATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGA
CAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATT
GCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCAT
TTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTAC
AAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAG
ATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTAC
GTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATT
TGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAA
ATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTT
ATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAA
GTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAAC
AGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTC
AAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGT
CGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCAT
CTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAAC
ACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTG
CACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCC
ATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAA
CTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAG
GCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCT
GATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGAT
GGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAA
CGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATG
ACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCA
CAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGA
ACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATC
ACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGG
CGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGAT
ACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGT
ATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTC
AGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACG
ACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCG
GTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTG
GTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCG
GCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCA
GAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGA
ACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGA
TCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGA
AACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTG
GTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGT
GGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTG
CGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATA
AACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT