(Circular) (Six-base) MAPSORT of: pFN21AB0485.seq Check: 7032 from: 1 to: 6289 pFN21AB0485 6289 bp With 182 enzymes: SgfI * October 20, 2009 10:59 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2999 3294 2999 Size: 295 5994 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4099 278 Size: 53 83 186 936 2563 2468 Fragments arranged by size: 2563 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3632 1937 Size: 1695 4594 Acc65I G'GTAC_C Cuts at: 3610 3610 Size: 6289 AclI AA'CG_TT Cuts at: 1563 4416 4789 1563 Size: 2853 373 3063 Fragments arranged by size: 3063 2853 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4352 5736 6004 1632 Size: 2720 1384 268 1917 Fragments arranged by size: 2720 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6092 AflIII A'CryG_T Cuts at: 1203 5188 6165 1203 Size: 3985 977 1327 Fragments arranged by size: 3985 1327 977 AgeI A'CCGG_T Cuts at: 5120 5120 Size: 6289 AhdI GACnn_n'nnGTC Cuts at: 2676 5018 2676 Size: 2342 3947 AleI CACnn'nnGTG Cuts at: 1166 2849 3563 1166 Size: 1683 714 3892 Fragments arranged by size: 3892 1683 714 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6289 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2093 2125 2093 Size: 32 6257 AlwNI CAG_nnn'CTG Cuts at: 2069 3499 4032 5604 2069 Size: 1430 533 1572 2754 Fragments arranged by size: 2754 1572 1430 533 ApaLI G'TGCA_C Cuts at: 4346 5502 4346 Size: 1156 5133 ApoI r'AATT_y Cuts at: 1493 3598 3824 3976 6085 1493 Size: 2105 226 152 2109 1697 Fragments arranged by size: 2109 2105 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4843 160 Size: 872 3811 1606 Fragments arranged by size: 3811 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6289 AvaI C'yCGr_G Cuts at: 1870 1943 2077 2120 2472 3060 3614 1870 Size: 73 134 43 352 588 554 4545 Fragments arranged by size: 4545 588 554 352 134 73 43 BamHI G'GATC_C Cuts at: 3619 3986 3619 Size: 367 5922 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2072 2338 3443 3538 3610 Size: 325 217 627 285 266 1105 95 72 Cuts at: 3610 5065 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1105 627 325 285 266 217 95 72 BanII G_rGCy'C Cuts at: 1391 2121 2301 2662 2803 2870 2887 3101 3308 Size: 730 180 361 141 67 17 214 207 Cuts at: 3308 3382 3608 1391 Size: 74 226 4072 Fragments arranged by size: 4072 730 361 226 214 207 180 141 74 67 17 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2823 928 Size: 971 924 4394 Fragments arranged by size: 4394 971 924 BbvCI CC'TCA_GC Cuts at: 2033 2033 Size: 6289 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4480 4514 1903 Size: 34 2543 34 3678 Fragments arranged by size: 3678 2543 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4181 5391 4181 Size: 1210 5079 BclI T'GATC_A Cuts at: 1548 1548 Size: 6289 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3096 3209 4900 Size: 107 122 71 631 605 1424 113 1691 Cuts at: 4900 136 Size: 1525 Fragments arranged by size: 1691 1525 1424 631 605 122 113 107 71 BglII A'GATC_T Cuts at: 2791 6284 2791 Size: 3493 2796 BlpI GC'TnA_GC Cuts at: 2567 3706 2567 Size: 1139 5150 Bme1580I G_kGCm'C Cuts at: 1790 3421 3446 3481 3543 3570 4350 5506 1790 Size: 1631 25 35 62 27 780 1156 2573 Fragments arranged by size: 2573 1631 1156 780 62 35 27 25 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6289 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2432 2665 3208 Size: 416 53 83 399 24 1015 233 543 Cuts at: 3208 3431 4020 4978 442 Size: 223 589 958 1753 Fragments arranged by size: 1753 1015 958 589 543 416 399 233 223 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6289 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2767 3200 2767 Size: 433 5856 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2305 2664 4949 1239 Size: 1066 359 2285 2579 Fragments arranged by size: 2579 2285 1066 359 Bpu10I CC'TnA_GC Cuts at: 2033 2033 Size: 6289 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4414 5279 5577 4414 Size: 865 298 5126 Fragments arranged by size: 5126 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2918 3173 4952 882 Size: 617 1419 255 1779 2219 Fragments arranged by size: 2219 1779 1419 617 255 BsaAI yAC'GTr Cuts at: 493 1204 3229 3490 4080 493 Size: 711 2025 261 590 2702 Fragments arranged by size: 2702 2025 711 590 261 BsaBI GATnn'nnATC Cuts at: 6283 6283 Size: 6289 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2247 4096 4478 5113 Size: 53 83 186 936 714 1849 382 635 Cuts at: 5113 275 Size: 1451 Fragments arranged by size: 1849 1451 936 714 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2590 2999 3528 4722 5120 5394 5541 5952 2590 Size: 409 529 1194 398 274 147 411 2927 Fragments arranged by size: 2927 1194 529 411 409 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6064 Fragments arranged by size: 6064 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2129 2453 1186 Size: 943 324 5022 Fragments arranged by size: 5022 943 324 BseYI C'CCAG_C Cuts at: 3289 3299 3543 5492 5988 3289 Size: 10 244 1949 496 3590 Fragments arranged by size: 3590 1949 496 244 10 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3087 3470 3488 3512 1163 Size: 573 1351 383 18 24 3940 Fragments arranged by size: 3940 1351 573 383 24 18 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2173 3469 3529 3739 4500 5528 Size: 275 186 172 1296 60 210 761 1028 Cuts at: 5528 1540 Size: 2301 Fragments arranged by size: 2301 1296 1028 761 275 210 186 172 60 BsiHKAI G_wGCw'C Cuts at: 3585 4350 4435 5506 3585 Size: 765 85 1071 4368 Fragments arranged by size: 4368 1071 765 85 BsmI GAATG_Cn' Cuts at: 2891 3800 3893 2891 Size: 909 93 5287 Fragments arranged by size: 5287 909 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2121 2301 2662 2803 2870 2887 3101 Size: 399 331 180 361 141 67 17 214 Cuts at: 3101 3308 3382 3421 3446 3481 3543 3570 3585 Size: 207 74 39 25 35 62 27 15 Cuts at: 3585 3608 4350 4435 5506 1391 Size: 23 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 361 331 214 207 180 141 85 74 67 62 39 35 27 25 23 17 15 BspEI T'CCGG_A Cuts at: 5952 5952 Size: 6289 BspHI T'CATG_A Cuts at: 4178 5908 4178 Size: 1730 4559 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2999 3294 3644 844 Size: 2155 295 350 3489 Fragments arranged by size: 3489 2155 350 295 BsrBI CCG'CTC Cuts at: 1394 1823 3555 4176 1394 Size: 429 1732 621 3507 Fragments arranged by size: 3507 1732 621 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2397 2526 2613 4784 4958 65 Size: 1183 1149 129 87 2171 174 1396 Fragments arranged by size: 2171 1396 1183 1149 174 129 87 BsrFI r'CCGG_y Cuts at: 1700 1810 2156 3039 3471 4933 5120 1700 Size: 110 346 883 432 1462 187 2869 Fragments arranged by size: 2869 1462 883 432 346 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6289 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6289 BssSI C'ACGA_G Cuts at: 2779 4349 5361 2779 Size: 1570 1012 3707 Fragments arranged by size: 3707 1570 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3499 794 Size: 2705 3584 BstBI TT'CG_AA Cuts at: 5093 5093 Size: 6289 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6289 BstXI CCAn_nnnn'nTGG Cuts at: 2329 2593 2329 Size: 264 6025 BstYI r'GATC_y Cuts at: 1972 2791 3619 3986 4371 4388 5840 5926 5938 Size: 819 828 367 385 17 1452 86 12 Cuts at: 5938 6284 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 828 819 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2094 2238 2739 3136 3570 513 Size: 552 939 90 144 501 397 434 3232 Fragments arranged by size: 3232 939 552 501 434 397 144 90 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2497 487 Size: 683 56 1271 4279 Fragments arranged by size: 4279 1271 683 56 BtsI GCAGTG_nn' Cuts at: 2605 2844 3805 4599 4619 2605 Size: 239 961 794 20 4275 Fragments arranged by size: 4275 961 794 239 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6289 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2509 2544 529 Size: 35 1945 35 4274 Fragments arranged by size: 4274 1945 35 35 DraI TTT'AAA Cuts at: 3593 3593 Size: 6289 DraIII CAC_nnn'GTG Cuts at: 2781 2781 Size: 6289 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2425 3187 5296 809 Size: 507 1109 762 2109 1802 Fragments arranged by size: 2109 1802 1109 762 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2170 2544 Size: 54 1425 50 275 18 177 163 374 Cuts at: 2544 2691 3150 3210 3573 3736 4625 6254 8 Size: 147 459 60 363 163 889 1629 43 Fragments arranged by size: 1629 1425 889 459 374 363 275 177 163 163 147 60 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2170 3736 1537 Size: 275 358 1566 4090 Fragments arranged by size: 4090 1566 358 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2962 3119 4219 1353 Size: 1609 157 1100 3423 Fragments arranged by size: 3423 1609 1100 157 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2057 3035 4876 5250 5396 387 Size: 1064 138 468 978 1841 374 146 1280 Fragments arranged by size: 1841 1280 1064 978 468 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2305 2664 4352 4949 5736 6004 1239 Size: 393 673 359 1688 597 787 268 1524 Fragments arranged by size: 1688 1524 787 673 597 393 359 268 EcoNI CCTnn'n_nnAGG Cuts at: 2036 2036 Size: 6289 EcoO109I rG'GnC_Cy Cuts at: 2125 2476 3496 2125 Size: 351 1020 4918 Fragments arranged by size: 4918 1020 351 EcoRI G'AATT_C Cuts at: 3598 3976 3598 Size: 378 5911 FspI TGC'GCA Cuts at: 4795 4795 Size: 6289 HaeII r_GCGC'y Cuts at: 1678 2749 5436 1678 Size: 1071 2687 2531 Fragments arranged by size: 2687 2531 1071 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2276 2308 2662 2694 2939 Size: 32 135 32 704 32 354 32 245 Cuts at: 2939 2971 4930 4962 5004 5036 1373 Size: 32 1959 32 42 32 2626 Fragments arranged by size: 2626 1959 704 354 245 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3633 3879 669 Size: 988 281 1695 246 3079 Fragments arranged by size: 3079 1695 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6289 HpaI GTT'AAC Cuts at: 3879 3879 Size: 6289 KpnI G_GTAC'C Cuts at: 3614 3614 Size: 6289 MfeI C'AATT_G Cuts at: 3888 3888 Size: 6289 MluI A'CGCG_T Cuts at: 6165 6165 Size: 6289 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2040 3078 5403 5587 1305 Size: 735 1038 2325 184 2007 Fragments arranged by size: 2325 2007 1038 735 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3152 3575 10 Size: 54 1425 343 1320 423 2724 Fragments arranged by size: 2724 1425 1320 423 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2591 2849 3563 4247 4606 4765 518 Size: 648 1425 258 714 684 359 159 2042 Fragments arranged by size: 2042 1425 714 684 648 359 258 159 MspA1I CmG'CkG Cuts at: 1606 2554 3484 3547 3705 4382 5530 5775 1606 Size: 948 930 63 158 677 1148 245 2120 Fragments arranged by size: 2120 1148 948 930 677 245 158 63 NaeI GCC'GGC Cuts at: 3041 3473 3041 Size: 432 5857 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4798 Fragments arranged by size: 4798 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6289 NgoMIV G'CCGG_C Cuts at: 3039 3471 3039 Size: 432 5857 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6289 NotI GC'GGCC_GC Cuts at: 3736 3736 Size: 6289 NspI r_CATG'y Cuts at: 1132 1229 3647 5192 1132 Size: 97 2418 1545 2229 Fragments arranged by size: 2418 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2836 1348 Size: 1488 4801 PciI A'CATG_T Cuts at: 5188 5188 Size: 6289 PflMI CCAn_nnn'nTGG Cuts at: 1108 2069 2781 3198 3289 1108 Size: 961 712 417 91 4108 Fragments arranged by size: 4108 961 712 417 91 PfoI T'CCnGG_A Cuts at: 2127 2941 2127 Size: 814 5475 PmeI GTTT'AAAC Cuts at: 3593 3593 Size: 6289 PmlI CAC'GTG Cuts at: 3490 3490 Size: 6289 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2762 2794 4358 4390 5871 5903 2762 Size: 32 1564 32 1481 32 3148 Fragments arranged by size: 3148 1564 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2125 2125 Size: 6289 PshAI GACnn'nnGTC Cuts at: 1613 3002 1613 Size: 1389 4900 PsiI TTA'TAA Cuts at: 3859 3859 Size: 6289 PstI C_TGCA'G Cuts at: 830 2148 3387 3641 830 Size: 1318 1239 254 3478 Fragments arranged by size: 3478 1318 1239 254 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6289 PvuII CAG'CTG Cuts at: 3484 3484 Size: 6289 SalI G'TCGA_C Cuts at: 1936 3631 1936 Size: 1695 4594 SapI GCTCTTCn'nnn_ Cuts at: 2962 2962 Size: 6289 SbfI CC_TGCA'GG Cuts at: 3641 3641 Size: 6289 ScaI AGT'ACT Cuts at: 4537 6249 4537 Size: 1712 4577 SfcI C'TryA_G Cuts at: 826 1045 2144 3383 3637 4772 5453 5644 826 Size: 219 1099 1239 254 1135 681 191 1471 Fragments arranged by size: 1471 1239 1135 1099 681 254 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 3209 3209 Size: 6289 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6289 SgrAI Cr'CCGG_yG Cuts at: 1700 3471 1700 Size: 1771 4518 SmaI CCC'GGG Cuts at: 1872 2079 2474 3062 3616 1872 Size: 207 395 588 554 4545 Fragments arranged by size: 4545 588 554 395 207 SmlI C'TyrA_G Cuts at: 820 1017 1943 4393 5294 5556 820 Size: 197 926 2450 901 262 1553 Fragments arranged by size: 2450 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4080 493 Size: 3587 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6289 SphI G_CATG'C Cuts at: 1132 3647 1132 Size: 2515 3774 SspI AAT'ATT Cuts at: 5 52 4213 5 Size: 47 4161 2081 Fragments arranged by size: 4161 2081 47 StuI AGG'CCT Cuts at: 1517 3263 1517 Size: 1746 4543 StyI C'CwwG_G Cuts at: 513 1065 2004 2729 2917 3728 513 Size: 552 939 725 188 811 3074 Fragments arranged by size: 3074 939 811 725 552 188 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2348 2999 3011 3281 4316 4333 4486 Size: 1255 768 651 12 270 1035 17 153 Cuts at: 4486 4671 5116 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 1035 768 651 445 270 185 153 17 12 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4535 6247 96 Size: 274 80 33 51 1443 2558 1712 138 Fragments arranged by size: 2558 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6249 6281 6249 Size: 32 6257 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6289 XbaI T'CTAG_A Cuts at: 3625 3625 Size: 6289 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3091 3091 Size: 6289 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6289 XmaI C'CCGG_G Cuts at: 1870 2077 2472 3060 3614 1870 Size: 207 395 588 554 4545 Fragments arranged by size: 4545 588 554 395 207 XmnI GAAnn'nnTTC Cuts at: 4418 4418 Size: 6289 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4097 276 Size: 53 83 186 936 2563 2468 Fragments arranged by size: 2563 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfoI SrfI SwaI