POLA2 ORF clone FHC06418

KOP
KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC06418

How to Order Order Information

Clone info.

Species

human

Clone Name

pFN21AB6157 Sequence (with vector) Restriction Map

Product ID

FHC06418

Flexi Clone PID

FXC06418

ORF Sequence

DNA sequence (1794 bp)
Protein sequence (598 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB464038]

Gene

KIBB6157

Symbol

POLA2

Description

polymerase (DNA directed), alpha 2, accessory subunit

Original Clone ID

cs00757

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1794 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 598 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS8098.1 0 99.8 POLA2 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR013627 15 248 PF08418 DNA polymerase alpha IPR007185 342 549 PF04042 DNA polymerase alpha/epsilon Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB6157 Download

>KIBB6157  1794 bp
ATGTCCGCATCCGCCCAGCAGCTGGCGGAGGAGCTGCAGATCTTCGGCCTAGACTGCGAG
GAGGCTCTAATTGAGAAATTGGTAGAGCTTTGTGTTCAGTATGGACAGAATGAGGAGGGA
ATGGTAGGCGAGCTTATAGCCTTCTGCACCAGCACACATAAAGTTGGCCTTACCTCAGAG
ATCCTGAACTCTTTTGAGCATGAGTTTCTGAGCAAAAGATTATCGAAAGCCAGGCATAGT
ACCTGCAAGGACAGTGGCCATGCAGGAGCTAGAGACATTGTTTCCATTCAAGAGCTAATT
GAAGTGGAAGAAGAAGAGGAAATCCTCTTGAACTCTTACACCACACCTTCAAAGGGTTCT
CAGAAGCGAGCTATCTCTACCCCAGAAACCCCCCTAACAAAAAGGAGTGTGTCAACTCGT
AGCCCCCATCAGCTACTCTCACCGTCAAGTTTCTCTCCAAGTGCTACTCCCTCCCAGAAA
TACAACTCACGAAGTAACCGAGGAGAAGTGGTTACCTCCTTCGGCTTAGCACAGGGAGTA
TCTTGGTCTGGGAGAGGAGGAGCTGGAAACATCAGCCTGAAGGTCTTGGGATGTCCAGAG
GCACTAACTGGGAGCTACAAATCCATGTTTCAGAAGCTCCCAGACATTCGAGAAGTTCTG
ACCTGTAAGATAGAAGAACTTGGCAGCGAACTCAAGGAACATTACAAGATTGAAGCTTTC
ACTCCTTTGCTAGCCCCAGCACAGGAGCCTGTCACTCTGCTGGGCCAGATTGGCTGTGAT
AGCAACGGGAAGCTGAACAACAAGTCAGTGATTCTCGAGGGAGACCGGGAACATTCCTCG
GGTGCTCAAATTCCAGTGGATTTATCTGAGCTTAAGGAATATTCTCTGTTTCCTGGACAG
GTTGTAATTATGGAAGGAATCAACACCACTGGTAGGAAACTTGTTGCCACCAAACTCTAC
GAGGGTGTGCCACTTCCATTTTATCAGCCCACTGAAGAGGATGCAGACTTTGAGCAAAGC
ATGGTCCTGGTTGCCTGTGGACCATACACCACATCTGACAGCATCACGTATGACCCCCTG
CTTGACCTGATTGCTGTCATCAACCATGACCGGCCAGATGTCTGCATCCTGTTTGGCCCT
TTCCTGGATGCTAAGCATGAACAGGTGGAGAATTGTCTACTGACAAGTCCATTTGAAGAC
ATTTTCAAGCAGTGTCTACGAACAATTATTGAAGGCACAAGAAGCTCCGGCTCCCACCTT
GTCTTTGTCCCGTCATTGAGAGATGTGCACCATGAGCCTGTGTACCCCCAGCCGCCTTTC
AGCTACTCCGATCTGTCTCGAGAGGACAAAAAGCAAGTACAGTTTGTGTCCGAGCCCTGC
AGCCTCTCCATAAACGGAGTGATCTTCGGCTTGACATCCACAGATCTGCTTTTCCACCTG
GGGGCCGAGGAGATCAGTAGTTCTTCCGGAACTTCAGACAGATTCAGCCGAATACTCAAG
CACATCTTGACCCAGAGGAGCTACTACCCACTCTACCCGCCCCAAGAAGACATGGCCATT
GACTATGAGTCGTTCTATGTTTACGCACAGCTGCCTGTCACCCCAGATGTCCTCATCATC
CCGTCAGAGCTGAGGTACTTCGTGAAGGATGTCCTCGGCTGTGTCTGTGTGAACCCTGGG
CGCCTTACCAAAGGGCAGGTGGGAGGCACCTTCGCCCGACTCTACCTTAGGAGGCCGGCA
GCGGACAGGGCAGAGAGGCAGAGCCCATGCATTGCTGTGCAGGTCGTCAGGATC

ORF Protein

Cloned ORF protein sequence for pFN21AB6157 Download

>KIBB6157  598 aa
MSASAQQLAEELQIFGLDCEEALIEKLVELCVQYGQNEEGMVGELIAFCTSTHKVGLTSE
ILNSFEHEFLSKRLSKARHSTCKDSGHAGARDIVSIQELIEVEEEEEILLNSYTTPSKGS
QKRAISTPETPLTKRSVSTRSPHQLLSPSSFSPSATPSQKYNSRSNRGEVVTSFGLAQGV
SWSGRGGAGNISLKVLGCPEALTGSYKSMFQKLPDIREVLTCKIEELGSELKEHYKIEAF
TPLLAPAQEPVTLLGQIGCDSNGKLNNKSVILEGDREHSSGAQIPVDLSELKEYSLFPGQ
VVIMEGINTTGRKLVATKLYEGVPLPFYQPTEEDADFEQSMVLVACGPYTTSDSITYDPL
LDLIAVINHDRPDVCILFGPFLDAKHEQVENCLLTSPFEDIFKQCLRTIIEGTRSSGSHL
VFVPSLRDVHHEPVYPQPPFSYSDLSREDKKQVQFVSEPCSLSINGVIFGLTSTDLLFHL
GAEEISSSSGTSDRFSRILKHILTQRSYYPLYPPQEDMAIDYESFYVYAQLPVTPDVLII
PSELRYFVKDVLGCVCVNPGRLTKGQVGGTFARLYLRRPAADRAERQSPCIAVQVVRI

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB6157 Download

>pFN21AB6157  6499 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGTCCGCATCCGCCCAGCAGCTGGCGGAGGAGCT
GCAGATCTTCGGCCTAGACTGCGAGGAGGCTCTAATTGAGAAATTGGTAGAGCTTTGTGT
TCAGTATGGACAGAATGAGGAGGGAATGGTAGGCGAGCTTATAGCCTTCTGCACCAGCAC
ACATAAAGTTGGCCTTACCTCAGAGATCCTGAACTCTTTTGAGCATGAGTTTCTGAGCAA
AAGATTATCGAAAGCCAGGCATAGTACCTGCAAGGACAGTGGCCATGCAGGAGCTAGAGA
CATTGTTTCCATTCAAGAGCTAATTGAAGTGGAAGAAGAAGAGGAAATCCTCTTGAACTC
TTACACCACACCTTCAAAGGGTTCTCAGAAGCGAGCTATCTCTACCCCAGAAACCCCCCT
AACAAAAAGGAGTGTGTCAACTCGTAGCCCCCATCAGCTACTCTCACCGTCAAGTTTCTC
TCCAAGTGCTACTCCCTCCCAGAAATACAACTCACGAAGTAACCGAGGAGAAGTGGTTAC
CTCCTTCGGCTTAGCACAGGGAGTATCTTGGTCTGGGAGAGGAGGAGCTGGAAACATCAG
CCTGAAGGTCTTGGGATGTCCAGAGGCACTAACTGGGAGCTACAAATCCATGTTTCAGAA
GCTCCCAGACATTCGAGAAGTTCTGACCTGTAAGATAGAAGAACTTGGCAGCGAACTCAA
GGAACATTACAAGATTGAAGCTTTCACTCCTTTGCTAGCCCCAGCACAGGAGCCTGTCAC
TCTGCTGGGCCAGATTGGCTGTGATAGCAACGGGAAGCTGAACAACAAGTCAGTGATTCT
CGAGGGAGACCGGGAACATTCCTCGGGTGCTCAAATTCCAGTGGATTTATCTGAGCTTAA
GGAATATTCTCTGTTTCCTGGACAGGTTGTAATTATGGAAGGAATCAACACCACTGGTAG
GAAACTTGTTGCCACCAAACTCTACGAGGGTGTGCCACTTCCATTTTATCAGCCCACTGA
AGAGGATGCAGACTTTGAGCAAAGCATGGTCCTGGTTGCCTGTGGACCATACACCACATC
TGACAGCATCACGTATGACCCCCTGCTTGACCTGATTGCTGTCATCAACCATGACCGGCC
AGATGTCTGCATCCTGTTTGGCCCTTTCCTGGATGCTAAGCATGAACAGGTGGAGAATTG
TCTACTGACAAGTCCATTTGAAGACATTTTCAAGCAGTGTCTACGAACAATTATTGAAGG
CACAAGAAGCTCCGGCTCCCACCTTGTCTTTGTCCCGTCATTGAGAGATGTGCACCATGA
GCCTGTGTACCCCCAGCCGCCTTTCAGCTACTCCGATCTGTCTCGAGAGGACAAAAAGCA
AGTACAGTTTGTGTCCGAGCCCTGCAGCCTCTCCATAAACGGAGTGATCTTCGGCTTGAC
ATCCACAGATCTGCTTTTCCACCTGGGGGCCGAGGAGATCAGTAGTTCTTCCGGAACTTC
AGACAGATTCAGCCGAATACTCAAGCACATCTTGACCCAGAGGAGCTACTACCCACTCTA
CCCGCCCCAAGAAGACATGGCCATTGACTATGAGTCGTTCTATGTTTACGCACAGCTGCC
TGTCACCCCAGATGTCCTCATCATCCCGTCAGAGCTGAGGTACTTCGTGAAGGATGTCCT
CGGCTGTGTCTGTGTGAACCCTGGGCGCCTTACCAAAGGGCAGGTGGGAGGCACCTTCGC
CCGACTCTACCTTAGGAGGCCGGCAGCGGACAGGGCAGAGAGGCAGAGCCCATGCATTGC
TGTGCAGGTCGTCAGGATCGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGA
GTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGT
TGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCA
GACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAA
TGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAAT
AAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGG
GAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATC
CTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGT
CAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACT
TTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATG
TATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT
ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCT
GTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCA
CGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCC
GAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCC
CGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTG
GTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTA
TGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATC
GGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTT
GATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATG
CCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCT
TCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGC
TCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCT
CGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTAC
ACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCC
TCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAG
CTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACA
TGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTT
TCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGC
GAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCT
CTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCG
TGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCA
AGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACT
ATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTA
ACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTA
ACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCT
TCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTT
TTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGA
TCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCA
TGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGC
ACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATT
AAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTT
ATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGC
CGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCAC
TTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCG
GCGTAGAGGATCGAGATCT