(Circular) (Six-base) MAPSORT of: pFN21AB6157.seq Check: 574 from: 1 to: 6499 pFN21AB6157 6499 bp With 182 enzymes: SgfI * October 20, 2009 10:57 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3691 3691 Size: 6499 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4309 278 Size: 53 83 186 936 2773 2468 Fragments arranged by size: 2773 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3181 3220 3842 1937 Size: 1244 39 622 4594 Fragments arranged by size: 4594 1244 622 39 Acc65I G'GTAC_C Cuts at: 3820 3820 Size: 6499 AclI AA'CG_TT Cuts at: 1563 4626 4999 1563 Size: 3063 373 3063 Fragments arranged by size: 3063 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2603 3018 3462 4562 5946 6214 1632 Size: 971 415 444 1100 1384 268 1917 Fragments arranged by size: 1917 1384 1100 971 444 415 268 AflII C'TTAA_G Cuts at: 820 1017 2876 820 Size: 197 1859 4443 Fragments arranged by size: 4443 1859 197 AflIII A'CryG_T Cuts at: 1203 5398 6375 1203 Size: 4195 977 1327 Fragments arranged by size: 4195 1327 977 AgeI A'CCGG_T Cuts at: 5330 5330 Size: 6499 AhdI GACnn_n'nnGTC Cuts at: 3571 5228 3571 Size: 1657 4842 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6499 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3797 1184 Size: 2613 3886 AlwNI CAG_nnn'CTG Cuts at: 2026 4242 5814 2026 Size: 2216 1572 2711 Fragments arranged by size: 2711 2216 1572 ApaLI G'TGCA_C Cuts at: 3290 4556 5712 3290 Size: 1266 1156 4077 Fragments arranged by size: 4077 1266 1156 ApoI r'AATT_y Cuts at: 1493 2853 3808 4034 4186 6295 1493 Size: 1360 955 226 152 2109 1697 Fragments arranged by size: 2109 1697 1360 955 226 152 AseI AT'TA_AT Cuts at: 160 1032 5053 160 Size: 872 4021 1606 Fragments arranged by size: 4021 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6499 AvaI C'yCGr_G Cuts at: 1870 1943 2819 2842 3342 3824 1870 Size: 73 876 23 500 482 4545 Fragments arranged by size: 4545 876 500 482 73 23 BamHI G'GATC_C Cuts at: 3829 4196 3829 Size: 367 6132 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3684 3710 3820 5275 618 Size: 325 217 627 1897 26 110 1455 1842 Fragments arranged by size: 1897 1842 1455 627 325 217 110 26 BanII G_rGCy'C Cuts at: 1391 3381 3770 3818 1391 Size: 1990 389 48 4072 Fragments arranged by size: 4072 1990 389 48 BbeI G_GCGC'C Cuts at: 3688 3688 Size: 6499 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3207 3558 928 Size: 971 1308 351 3869 Fragments arranged by size: 3869 1308 971 351 BbvCI CC'TCA_GC Cuts at: 3635 3635 Size: 6499 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4690 4724 1903 Size: 34 2753 34 3678 Fragments arranged by size: 3678 2753 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4391 5601 4391 Size: 1210 5289 BclI T'GATC_A Cuts at: 1548 1548 Size: 6499 BfrBI ATG'CAT Cuts at: 3774 3774 Size: 6499 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2775 5110 136 Size: 107 122 71 631 605 1103 2335 1525 Fragments arranged by size: 2335 1525 1103 631 605 122 107 71 BglII A'GATC_T Cuts at: 2043 3427 6494 2043 Size: 1384 3067 2048 Fragments arranged by size: 3067 2048 1384 BlpI GC'TnA_GC Cuts at: 2530 3156 3916 2530 Size: 626 760 5113 Fragments arranged by size: 5113 760 626 Bme1580I G_kGCm'C Cuts at: 1790 3294 4560 5716 1790 Size: 1504 1266 1156 2573 Fragments arranged by size: 2573 1504 1266 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6499 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2622 4230 5188 Size: 416 53 83 399 24 1205 1608 958 Cuts at: 5188 442 Size: 1753 Fragments arranged by size: 1753 1608 1205 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2738 1056 Size: 1682 4817 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 5159 1239 Size: 3920 2579 Bpu10I CC'TnA_GC Cuts at: 3635 3635 Size: 6499 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2681 3485 4624 5489 5787 2681 Size: 804 1139 865 298 3393 Fragments arranged by size: 3393 1139 865 804 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2820 5162 882 Size: 617 1321 2342 2219 Fragments arranged by size: 2342 2219 1321 617 BsaAI yAC'GTr Cuts at: 493 1204 3072 4290 493 Size: 711 1868 1218 2702 Fragments arranged by size: 2702 1868 1218 711 BsaBI GATnn'nnATC Cuts at: 6493 6493 Size: 6499 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3685 4306 4688 5323 Size: 53 83 186 936 2152 621 382 635 Cuts at: 5323 275 Size: 1451 Fragments arranged by size: 2152 1451 936 635 621 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3470 4932 5330 5604 5751 6162 3470 Size: 1462 398 274 147 411 3807 Fragments arranged by size: 3807 1462 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2499 2529 1178 Size: 30 165 30 1096 30 5148 Fragments arranged by size: 5148 1096 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2048 2078 2132 2520 2574 2577 3467 3535 Size: 862 30 54 388 54 3 890 68 Cuts at: 3535 1186 Size: 4150 Fragments arranged by size: 4150 890 862 388 68 54 54 30 3 BseYI C'CCAG_C Cuts at: 2019 2740 2764 3312 5702 6198 2019 Size: 721 24 548 2390 496 2320 Fragments arranged by size: 2390 2320 721 548 496 24 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2134 3803 1163 Size: 573 398 1669 3859 Fragments arranged by size: 3859 1669 573 398 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3949 4710 5738 1540 Size: 275 186 1948 761 1028 2301 Fragments arranged by size: 2301 1948 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2851 3294 4560 4645 5716 2851 Size: 443 1266 85 1071 3634 Fragments arranged by size: 3634 1266 1071 443 85 BsmI GAATG_Cn' Cuts at: 4010 4103 4010 Size: 93 6406 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2851 3294 3381 3770 3818 4560 4645 Size: 399 1061 443 87 389 48 742 85 Cuts at: 4645 5716 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 1061 742 443 399 389 87 85 48 BspEI T'CCGG_A Cuts at: 3470 6162 3470 Size: 2692 3807 BspHI T'CATG_A Cuts at: 4388 6118 4388 Size: 1730 4769 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2255 3691 3775 3854 844 Size: 1411 1436 84 79 3489 Fragments arranged by size: 3489 1436 1411 84 79 BsrBI CCG'CTC Cuts at: 1394 1823 4386 1394 Size: 429 2563 3507 Fragments arranged by size: 3507 2563 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3774 4994 5168 65 Size: 1183 2526 1220 174 1396 Fragments arranged by size: 2526 1396 1220 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3114 3739 5143 5330 1700 Size: 110 1304 625 1404 187 2869 Fragments arranged by size: 2869 1404 1304 625 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6499 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6499 BssSI C'ACGA_G Cuts at: 4559 5571 4559 Size: 1012 5487 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6499 BstBI TT'CG_AA Cuts at: 5303 5303 Size: 6499 BstEII G'GTnAC_C Cuts at: 1184 2515 1184 Size: 1331 5168 BstYI r'GATC_y Cuts at: 1972 2043 2184 3427 3829 4196 4581 4598 6050 Size: 71 141 1243 402 367 385 17 1452 Cuts at: 6050 6136 6148 6494 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1243 402 385 367 346 141 86 71 17 12 Bsu36I CC'TnA_GG Cuts at: 3731 3731 Size: 6499 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5947 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5760 Fragments arranged by size: 5760 683 56 BtsI GCAGTG_nn' Cuts at: 3221 4015 4809 4829 3221 Size: 794 794 20 4891 Fragments arranged by size: 4891 794 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6499 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2239 2274 529 Size: 35 1675 35 4754 Fragments arranged by size: 4754 1675 35 35 DraI TTT'AAA Cuts at: 3803 3803 Size: 6499 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5506 809 Size: 507 4190 1802 Fragments arranged by size: 4190 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2260 3116 3558 Size: 54 1425 50 275 18 430 856 442 Cuts at: 3558 3946 4835 6464 8 Size: 388 889 1629 43 Fragments arranged by size: 1629 1425 889 856 442 430 388 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3946 1537 Size: 275 2134 4090 Fragments arranged by size: 4090 2134 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2313 2994 4429 1353 Size: 960 681 1435 3423 Fragments arranged by size: 3423 1435 960 681 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2005 2045 5086 5460 5606 387 Size: 1064 138 416 40 3041 374 146 1280 Fragments arranged by size: 3041 1280 1064 416 374 146 138 40 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2603 3018 3462 4562 5159 5946 6214 Size: 393 971 415 444 1100 597 787 268 Cuts at: 6214 1239 Size: 1524 Fragments arranged by size: 1524 1100 971 787 597 444 415 393 268 EcoRI G'AATT_C Cuts at: 3808 4186 3808 Size: 378 6121 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2189 3526 2189 Size: 1337 5162 FspI TGC'GCA Cuts at: 5005 5005 Size: 6499 HaeII r_GCGC'y Cuts at: 1678 3688 5646 1678 Size: 2010 1958 2531 Fragments arranged by size: 2531 2010 1958 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3557 3589 5140 5172 5214 Size: 32 135 32 1985 32 1551 32 42 Cuts at: 5214 5246 1373 Size: 32 2626 Fragments arranged by size: 2626 1985 1551 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2418 3843 4089 669 Size: 988 281 480 1425 246 3079 Fragments arranged by size: 3079 1425 988 480 281 246 HindIII A'AGCT_T Cuts at: 748 2718 748 Size: 1970 4529 HpaI GTT'AAC Cuts at: 4089 4089 Size: 6499 KasI G'GCGC_C Cuts at: 3684 3684 Size: 6499 KpnI G_GTAC'C Cuts at: 3824 3824 Size: 6499 MfeI C'AATT_G Cuts at: 4098 4098 Size: 6499 MluI A'CGCG_T Cuts at: 6375 6375 Size: 6499 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5613 5797 1305 Size: 4308 184 2007 Fragments arranged by size: 4308 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2262 3560 10 Size: 54 1425 343 430 1298 2949 Fragments arranged by size: 2949 1425 1298 430 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3779 4457 4816 4975 518 Size: 648 2613 678 359 159 2042 Fragments arranged by size: 2613 2042 678 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2026 3595 3746 3915 4592 5740 5985 1606 Size: 420 1569 151 169 677 1148 245 2120 Fragments arranged by size: 2120 1569 1148 677 420 245 169 151 NaeI GCC'GGC Cuts at: 3741 3741 Size: 6499 NarI GG'CG_CC Cuts at: 3685 3685 Size: 6499 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5947 NdeI CA'TA_TG Cuts at: 387 387 Size: 6499 NgoMIV G'CCGG_C Cuts at: 3739 3739 Size: 6499 NheI G'CTAG_C Cuts at: 1052 2734 1052 Size: 1682 4817 NotI GC'GGCC_GC Cuts at: 3946 3946 Size: 6499 NsiI A_TGCA'T Cuts at: 3776 3776 Size: 6499 NspI r_CATG'y Cuts at: 1132 1229 3857 5402 1132 Size: 97 2628 1545 2229 Fragments arranged by size: 2628 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3680 1348 Size: 2332 4167 PciI A'CATG_T Cuts at: 5398 5398 Size: 6499 PflMI CCAn_nnn'nTGG Cuts at: 1108 2026 1108 Size: 918 5581 PfoI T'CCnGG_A Cuts at: 2896 3147 2896 Size: 251 6248 PmeI GTTT'AAAC Cuts at: 3803 3803 Size: 6499 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4568 4600 6081 6113 4568 Size: 32 1481 32 4954 Fragments arranged by size: 4954 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6499 PsiI TTA'TAA Cuts at: 4069 4069 Size: 6499 PstI C_TGCA'G Cuts at: 830 2043 3386 3851 830 Size: 1213 1343 465 3478 Fragments arranged by size: 3478 1343 1213 465 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6499 PvuII CAG'CTG Cuts at: 2026 3595 2026 Size: 1569 4930 SalI G'TCGA_C Cuts at: 1936 3841 1936 Size: 1905 4594 SbfI CC_TGCA'GG Cuts at: 3851 3851 Size: 6499 ScaI AGT'ACT Cuts at: 4747 6459 4747 Size: 1712 4787 SfcI C'TryA_G Cuts at: 826 1045 2039 3382 3847 4982 5663 5854 826 Size: 219 994 1343 465 1135 681 191 1471 Fragments arranged by size: 1471 1343 1135 994 681 465 219 191 SfoI GGC'GCC Cuts at: 3686 3686 Size: 6499 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6499 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6499 SmaI CCC'GGG Cuts at: 1872 3826 1872 Size: 1954 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2696 2819 2876 3342 3500 4603 Size: 197 926 753 123 57 466 158 1103 Cuts at: 4603 5504 5766 820 Size: 901 262 1553 Fragments arranged by size: 1553 1103 926 901 753 466 262 197 158 123 57 SnaBI TAC'GTA Cuts at: 493 4290 493 Size: 3797 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6499 SphI G_CATG'C Cuts at: 1132 3857 1132 Size: 2725 3774 SspI AAT'ATT Cuts at: 5 52 2885 4423 5 Size: 47 2833 1538 2081 Fragments arranged by size: 2833 2081 1538 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6499 StyI C'CwwG_G Cuts at: 513 1065 3938 513 Size: 552 2873 3074 Fragments arranged by size: 3074 2873 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4526 4543 4696 4881 5326 325 Size: 1255 2946 17 153 185 445 1498 Fragments arranged by size: 2946 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3361 4745 6457 Size: 274 80 33 51 1443 1384 1384 1712 Cuts at: 6457 96 Size: 138 Fragments arranged by size: 1712 1443 1384 1384 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6459 6491 6459 Size: 32 6467 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6499 XbaI T'CTAG_A Cuts at: 3835 3835 Size: 6499 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3700 3700 Size: 6499 XhoI C'TCGA_G Cuts at: 1943 2819 3342 1943 Size: 876 523 5100 Fragments arranged by size: 5100 876 523 XmaI C'CCGG_G Cuts at: 1870 3824 1870 Size: 1954 4545 XmnI GAAnn'nnTTC Cuts at: 4628 4628 Size: 6499 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4307 276 Size: 53 83 186 936 2773 2468 Fragments arranged by size: 2773 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI NruI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI