GPR15 ORF clone FHC05686

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC05686

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Clone info.

Species

human

Clone Name

pFN21AE9405 Sequence (with vector) Restriction Map

Product ID

FHC05686

ORF Sequence

DNA sequence (1080 bp)
Protein sequence (360 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE9405

Symbol

GPR15

Alias : BOB

Description

G protein-coupled receptor 15

Original Clone ID

cp99405

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1080 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 360 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS2931.1 1.5e-116 100.0 GPR15 CCDS1405.1 1e-25 36.3 GPR25 CCDS3137.1 1.1e-22 32.9 AGTR1 CCDS82855.1 1.1e-22 32.9 AGTR1 CCDS82854.1 1.1e-22 32.9 AGTR1 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR000276 35 59 PR00237 GPCR IPR000276 68 89 PR00237 GPCR IPR002286 106 123 PR01157 P2 purinoceptor IPR000276 113 135 PR00237 GPCR IPR002286 130 140 PR01157 P2 purinoceptor IPR000276 149 170 PR00237 GPCR IPR000276 196 219 PR00237 GPCR IPR002286 214 225 PR01157 P2 purinoceptor IPR000276 239 263 PR00237 GPCR IPR002286 253 264 PR01157 P2 purinoceptor IPR000276 284 310 PR00237 GPCR HMMPfam IPR000276 50 302 PF00001 GPCR ProfileScan IPR017452 50 302 PS50262 GPCR
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 34 VFLPVFYTAVFLTGVLGNLVLMG 56 PRIMARY 23 2 67 LIDIFIINLAASDFIFLVTLPLW 89 PRIMARY 23 3 118 HCSVLLLTCMSVDRYLAIVWPVV 140 SECONDARY 23 4 151 YVVCASIWFISCLLGLPTLLSRE 173 PRIMARY 23 5 195 WSLVALIFTFFVPLLSIVTCYCC 217 PRIMARY 23 6 243 IFIVVAAFLVSWLPFNTFKFLAI 265 PRIMARY 23 7 288 GPLAFANSCVNPFIYYIFDSYI 309 SECONDARY 22
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_005281 360 601166 100.0 100.0 NP_005289 361 602174 36.3 80.6 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE9405 Download

>KIEE9405  1080 bp
ATGGACCCAGAAGAAACTTCAGTTTATTTGGATTATTACTATGCTACGAGCCCAAACTCT
GACATCAGGGAGACCCACTCCCATGTTCCTTACACCTCTGTCTTCCTTCCAGTCTTTTAC
ACAGCTGTGTTCCTGACTGGAGTGCTGGGGAACCTTGTTCTCATGGGAGCGTTGCATTTC
AAACCCGGCAGCCGAAGACTGATCGACATCTTTATCATCAATCTGGCTGCCTCTGACTTC
ATTTTTCTTGTCACATTGCCTCTCTGGGTGGATAAAGAAGCATCTCTAGGACTGTGGAGG
ACGGGCTCCTTCCTGTGCAAAGGGAGCTCCTACATGATCTCCGTCAATATGCACTGCAGT
GTCCTCCTGCTCACTTGCATGAGTGTTGACCGCTACCTGGCCATTGTGTGGCCAGTCGTA
TCCAGGAAATTCAGAAGGACAGACTGTGCATATGTAGTCTGTGCCAGCATCTGGTTTATC
TCCTGCCTGCTGGGGTTGCCTACTCTTCTGTCCAGGGAGCTCACGCTGATTGATGATAAG
CCATACTGTGCAGAGAAAAAGGCAACTCCAATTAAACTCATATGGTCCCTGGTGGCCTTA
ATTTTCACCTTTTTTGTCCCTTTGTTGAGCATTGTGACCTGCTACTGTTGCATTGCAAGG
AAGCTGTGTGCCCATTACCAGCAATCAGGAAAGCACAACAAAAAGCTGAAGAAATCTATA
AAGATCATCTTTATTGTCGTGGCAGCCTTTCTTGTCTCCTGGCTGCCCTTCAATACTTTC
AAGTTCCTGGCCATTGTCTCTGGGTTGCGGCAAGAACACTATTTACCCTCAGCTATTCTT
CAGCTTGGTATGGAGGTGAGTGGACCCTTGGCATTTGCCAACAGCTGTGTCAACCCTTTC
ATTTACTATATCTTCGACAGCTACATCCGCCGGGCCATTGTCCACTGCTTGTGCCCTTGC
CTGAAAAACTATGACTTTGGGAGTAGCACTGAGACATCAGATAGTCACCTCACTAAGGCT
CTCTCCACCTTCATTCATGCAGAAGATTTTGCCAGGAGGAGGAAGAGGTCTGTGTCACTC

ORF Protein

Cloned ORF protein sequence for pFN21AE9405 Download

>KIEE9405  360 aa
MDPEETSVYLDYYYATSPNSDIRETHSHVPYTSVFLPVFYTAVFLTGVLGNLVLMGALHF
KPGSRRLIDIFIINLAASDFIFLVTLPLWVDKEASLGLWRTGSFLCKGSSYMISVNMHCS
VLLLTCMSVDRYLAIVWPVVSRKFRRTDCAYVVCASIWFISCLLGLPTLLSRELTLIDDK
PYCAEKKATPIKLIWSLVALIFTFFVPLLSIVTCYCCIARKLCAHYQQSGKHNKKLKKSI
KIIFIVVAAFLVSWLPFNTFKFLAIVSGLRQEHYLPSAILQLGMEVSGPLAFANSCVNPF
IYYIFDSYIRRAIVHCLCPCLKNYDFGSSTETSDSHLTKALSTFIHAEDFARRRKRSVSL

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE9405 Download

>pFN21AE9405  5785 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGACCCAGAAGAAACTTCAGTTTATTTGGATTA
TTACTATGCTACGAGCCCAAACTCTGACATCAGGGAGACCCACTCCCATGTTCCTTACAC
CTCTGTCTTCCTTCCAGTCTTTTACACAGCTGTGTTCCTGACTGGAGTGCTGGGGAACCT
TGTTCTCATGGGAGCGTTGCATTTCAAACCCGGCAGCCGAAGACTGATCGACATCTTTAT
CATCAATCTGGCTGCCTCTGACTTCATTTTTCTTGTCACATTGCCTCTCTGGGTGGATAA
AGAAGCATCTCTAGGACTGTGGAGGACGGGCTCCTTCCTGTGCAAAGGGAGCTCCTACAT
GATCTCCGTCAATATGCACTGCAGTGTCCTCCTGCTCACTTGCATGAGTGTTGACCGCTA
CCTGGCCATTGTGTGGCCAGTCGTATCCAGGAAATTCAGAAGGACAGACTGTGCATATGT
AGTCTGTGCCAGCATCTGGTTTATCTCCTGCCTGCTGGGGTTGCCTACTCTTCTGTCCAG
GGAGCTCACGCTGATTGATGATAAGCCATACTGTGCAGAGAAAAAGGCAACTCCAATTAA
ACTCATATGGTCCCTGGTGGCCTTAATTTTCACCTTTTTTGTCCCTTTGTTGAGCATTGT
GACCTGCTACTGTTGCATTGCAAGGAAGCTGTGTGCCCATTACCAGCAATCAGGAAAGCA
CAACAAAAAGCTGAAGAAATCTATAAAGATCATCTTTATTGTCGTGGCAGCCTTTCTTGT
CTCCTGGCTGCCCTTCAATACTTTCAAGTTCCTGGCCATTGTCTCTGGGTTGCGGCAAGA
ACACTATTTACCCTCAGCTATTCTTCAGCTTGGTATGGAGGTGAGTGGACCCTTGGCATT
TGCCAACAGCTGTGTCAACCCTTTCATTTACTATATCTTCGACAGCTACATCCGCCGGGC
CATTGTCCACTGCTTGTGCCCTTGCCTGAAAAACTATGACTTTGGGAGTAGCACTGAGAC
ATCAGATAGTCACCTCACTAAGGCTCTCTCCACCTTCATTCATGCAGAAGATTTTGCCAG
GAGGAGGAAGAGGTCTGTGTCACTCGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCC
TCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAG
CTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTT
CGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGA
AAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGC
TGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAG
ATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAA
TGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATC
CGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGT
GGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCA
AATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGG
AAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGC
CTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTG
GGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTT
CGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTA
TTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAAT
GACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGA
GAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACA
ACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACT
CGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACC
ACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACT
CTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTT
CTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGT
GGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTT
ATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATA
GGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGG
TATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAA
AGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGG
CGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGA
GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCG
TGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGG
GAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTC
GCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCG
GTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCA
CTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGT
GGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAG
TTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCG
GTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATC
CTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTT
TGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAG
GAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAG
TGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTA
AAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTC
TGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAA
ATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATG
CGTCCGGCGTAGAGGATCGAGATCT