(Circular) (Six-base) MAPSORT of: pFN21AE9405.seq Check: 1779 from: 1 to: 5785 pFN21AE9405 5785 bp With 182 enzymes: SgfI * December 28, 2011 15:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3595 278 Size: 53 83 186 936 2059 2468 Fragments arranged by size: 2468 2059 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3128 1937 Size: 1191 4594 Acc65I G'GTAC_C Cuts at: 3106 3106 Size: 5785 AclI AA'CG_TT Cuts at: 1563 3912 4285 1563 Size: 2349 373 3063 Fragments arranged by size: 3063 2349 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2007 2732 2828 3848 5232 5500 1632 Size: 375 725 96 1020 1384 268 1917 Fragments arranged by size: 1917 1384 1020 725 375 268 96 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5588 AflIII A'CryG_T Cuts at: 1203 4684 5661 1203 Size: 3481 977 1327 Fragments arranged by size: 3481 1327 977 AgeI A'CCGG_T Cuts at: 4616 4616 Size: 5785 AhdI GACnn_n'nnGTC Cuts at: 4514 4514 Size: 5785 AleI CACnn'nnGTG Cuts at: 1166 2129 2361 1166 Size: 963 232 4590 Fragments arranged by size: 4590 963 232 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5785 AlwNI CAG_nnn'CTG Cuts at: 2475 3528 5100 2475 Size: 1053 1572 3160 Fragments arranged by size: 3160 1572 1053 ApaLI G'TGCA_C Cuts at: 3842 4998 3842 Size: 1156 4629 ApoI r'AATT_y Cuts at: 1493 2432 3094 3320 3472 5581 1493 Size: 939 662 226 152 2109 1697 Fragments arranged by size: 2109 1697 939 662 226 152 AseI AT'TA_AT Cuts at: 160 1032 4339 160 Size: 872 3307 1606 Fragments arranged by size: 3307 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5785 AvaI C'yCGr_G Cuts at: 1870 1943 3110 1870 Size: 73 1167 4545 Fragments arranged by size: 4545 1167 73 BamHI G'GATC_C Cuts at: 3115 3482 3115 Size: 367 5418 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3106 4561 618 Size: 325 217 627 1319 1455 1842 Fragments arranged by size: 1842 1455 1319 627 325 217 BanII G_rGCy'C Cuts at: 1391 2057 2312 2333 2526 3104 1391 Size: 666 255 21 193 578 4072 Fragments arranged by size: 4072 666 578 255 193 21 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2098 2206 928 Size: 971 199 108 4507 Fragments arranged by size: 4507 971 199 108 BbvCI CC'TCA_GC Cuts at: 2833 2833 Size: 5785 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3976 4010 1903 Size: 34 2039 34 3678 Fragments arranged by size: 3678 2039 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2434 3677 4887 2434 Size: 1243 1210 3332 Fragments arranged by size: 3332 1243 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5785 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4396 136 Size: 107 122 71 631 605 2724 1525 Fragments arranged by size: 2724 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5780 5780 Size: 5785 BlpI GC'TnA_GC Cuts at: 3202 3202 Size: 5785 Bme1580I G_kGCm'C Cuts at: 1790 2677 2960 3846 5002 1790 Size: 887 283 886 1156 2573 Fragments arranged by size: 2573 1156 887 886 283 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5785 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3516 4474 442 Size: 416 53 83 399 24 2099 958 1753 Fragments arranged by size: 2099 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5785 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2098 2098 Size: 5785 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2163 4445 1239 Size: 924 2282 2579 Fragments arranged by size: 2579 2282 924 Bpu10I CC'TnA_GC Cuts at: 2833 2833 Size: 5785 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3910 4775 5073 3910 Size: 865 298 4622 Fragments arranged by size: 4622 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2069 4448 882 Size: 617 570 2379 2219 Fragments arranged by size: 2379 2219 617 570 BsaAI yAC'GTr Cuts at: 493 1204 3576 493 Size: 711 2372 2702 Fragments arranged by size: 2702 2372 711 BsaBI GATnn'nnATC Cuts at: 2210 5779 2210 Size: 3569 2216 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3592 3974 4609 275 Size: 53 83 186 936 2059 382 635 1451 Fragments arranged by size: 2059 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4218 4616 4890 5037 5448 4218 Size: 398 274 147 411 4555 Fragments arranged by size: 4555 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2327 2357 1178 Size: 30 165 30 924 30 4606 Fragments arranged by size: 4606 924 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3076 1186 Size: 1890 3895 BseYI C'CCAG_C Cuts at: 2149 2494 4988 5484 2149 Size: 345 2494 496 2450 Fragments arranged by size: 2494 2450 496 345 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2574 1163 Size: 573 838 4374 Fragments arranged by size: 4374 838 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3235 3996 5024 1540 Size: 275 186 1234 761 1028 2301 Fragments arranged by size: 2301 1234 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2333 2526 3846 3931 5002 2333 Size: 193 1320 85 1071 3116 Fragments arranged by size: 3116 1320 1071 193 85 BsmI GAATG_Cn' Cuts at: 3296 3389 3296 Size: 93 5692 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2057 2312 2333 2526 2677 2960 3104 Size: 399 267 255 21 193 151 283 144 Cuts at: 3104 3846 3931 5002 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 283 267 255 193 151 144 85 21 BspEI T'CCGG_A Cuts at: 5448 5448 Size: 5785 BspHI T'CATG_A Cuts at: 3674 5404 3674 Size: 1730 4055 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2651 3140 844 Size: 1807 489 3489 Fragments arranged by size: 3489 1807 489 BsrBI CCG'CTC Cuts at: 1394 1823 3672 1394 Size: 429 1849 3507 Fragments arranged by size: 3507 1849 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2258 2655 4280 4454 65 Size: 1183 1010 397 1625 174 1396 Fragments arranged by size: 1625 1396 1183 1010 397 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4429 4616 1700 Size: 110 2619 187 2869 Fragments arranged by size: 2869 2619 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5785 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5785 BssSI C'ACGA_G Cuts at: 3845 4857 3845 Size: 1012 4773 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5785 BstBI TT'CG_AA Cuts at: 4589 4589 Size: 5785 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5785 BstYI r'GATC_y Cuts at: 1972 3115 3482 3867 3884 5336 5422 5434 5780 Size: 1143 367 385 17 1452 86 12 346 Cuts at: 5780 1972 Size: 1977 Fragments arranged by size: 1977 1452 1143 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4294 Fragments arranged by size: 4294 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5046 Fragments arranged by size: 5046 683 56 BtsI GCAGTG_nn' Cuts at: 2356 2368 2947 3301 4095 4115 2356 Size: 12 579 354 794 20 4026 Fragments arranged by size: 4026 794 579 354 20 12 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5785 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5750 DraI TTT'AAA Cuts at: 3089 3089 Size: 5785 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4792 809 Size: 507 3476 1802 Fragments arranged by size: 3476 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2403 2414 2793 Size: 54 1425 50 275 18 573 11 379 Cuts at: 2793 3232 4121 5750 8 Size: 439 889 1629 43 Fragments arranged by size: 1629 1425 889 573 439 379 275 54 50 43 18 11 EagI C'GGCC_G Cuts at: 1537 1812 3232 1537 Size: 275 1420 4090 Fragments arranged by size: 4090 1420 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2514 3062 3715 1353 Size: 1161 548 653 3423 Fragments arranged by size: 3423 1161 653 548 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2921 4372 4746 4892 387 Size: 1064 138 1332 1451 374 146 1280 Fragments arranged by size: 1451 1332 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2331 2524 2331 Size: 193 5592 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2007 2163 2732 2828 3848 4445 5232 Size: 393 375 156 569 96 1020 597 787 Cuts at: 5232 5500 1239 Size: 268 1524 Fragments arranged by size: 1524 1020 787 597 569 393 375 268 156 96 EcoNI CCTnn'n_nnAGG Cuts at: 3017 3017 Size: 5785 EcoRI G'AATT_C Cuts at: 3094 3472 3094 Size: 378 5407 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2749 2749 Size: 5785 FspI TGC'GCA Cuts at: 4291 4291 Size: 5785 HaeII r_GCGC'y Cuts at: 1678 4932 1678 Size: 3254 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4426 4458 4500 4532 1373 Size: 32 135 32 2854 32 42 32 2626 Fragments arranged by size: 2854 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2392 2896 3129 3375 669 Size: 988 281 454 504 233 246 3079 Fragments arranged by size: 3079 988 504 454 281 246 233 HindIII A'AGCT_T Cuts at: 748 748 Size: 5785 HpaI GTT'AAC Cuts at: 3375 3375 Size: 5785 KpnI G_GTAC'C Cuts at: 3110 3110 Size: 5785 MfeI C'AATT_G Cuts at: 3384 3384 Size: 5785 MluI A'CGCG_T Cuts at: 5661 5661 Size: 5785 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4899 5083 1305 Size: 3594 184 2007 Fragments arranged by size: 3594 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2405 2416 2795 10 Size: 54 1425 343 573 11 379 3000 Fragments arranged by size: 3000 1425 573 379 343 54 11 MslI CAynn'nnrTG Cuts at: 518 1166 2085 2129 2361 2381 3743 4102 4261 Size: 648 919 44 232 20 1362 359 159 Cuts at: 4261 518 Size: 2042 Fragments arranged by size: 2042 1362 919 648 359 232 159 44 20 MspA1I CmG'CkG Cuts at: 1606 2129 2889 3201 3878 5026 5271 1606 Size: 523 760 312 677 1148 245 2120 Fragments arranged by size: 2120 1148 760 677 523 312 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4294 Fragments arranged by size: 4294 939 552 NdeI CA'TA_TG Cuts at: 387 2455 2585 387 Size: 2068 130 3587 Fragments arranged by size: 3587 2068 130 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5785 NotI GC'GGCC_GC Cuts at: 3232 3232 Size: 5785 NspI r_CATG'y Cuts at: 1132 1229 3143 4688 1132 Size: 97 1914 1545 2229 Fragments arranged by size: 2229 1914 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5785 PciI A'CATG_T Cuts at: 4684 4684 Size: 5785 PflMI CCAn_nnn'nTGG Cuts at: 1108 2412 2475 1108 Size: 1304 63 4418 Fragments arranged by size: 4418 1304 63 PfoI T'CCnGG_A Cuts at: 2426 2426 Size: 5785 PmeI GTTT'AAAC Cuts at: 3089 3089 Size: 5785 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3854 3886 5367 5399 3854 Size: 32 1481 32 4240 Fragments arranged by size: 4240 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5785 PsiI TTA'TAA Cuts at: 3355 3355 Size: 5785 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2811 2843 2811 Size: 32 5753 PstI C_TGCA'G Cuts at: 830 2363 3137 830 Size: 1533 774 3478 Fragments arranged by size: 3478 1533 774 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5785 PvuII CAG'CTG Cuts at: 2129 2889 2129 Size: 760 5025 SacI G_AGCT'C Cuts at: 2333 2526 2333 Size: 193 5592 SalI G'TCGA_C Cuts at: 1936 3127 1936 Size: 1191 4594 SbfI CC_TGCA'GG Cuts at: 3137 3137 Size: 5785 ScaI AGT'ACT Cuts at: 4033 5745 4033 Size: 1712 4073 SfcI C'TryA_G Cuts at: 826 1045 2359 3133 4268 4949 5140 826 Size: 219 1314 774 1135 681 191 1471 Fragments arranged by size: 1471 1314 1135 774 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5785 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5785 SmaI CCC'GGG Cuts at: 1872 3112 1872 Size: 1240 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3889 4790 5052 820 Size: 197 926 1946 901 262 1553 Fragments arranged by size: 1946 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3576 493 Size: 3083 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5785 SphI G_CATG'C Cuts at: 1132 3143 1132 Size: 2011 3774 SspI AAT'ATT Cuts at: 5 52 3709 5 Size: 47 3657 2081 Fragments arranged by size: 3657 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5785 StyI C'CwwG_G Cuts at: 513 1065 2004 2871 3224 513 Size: 552 939 867 353 3074 Fragments arranged by size: 3074 939 867 552 353 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2260 3812 3829 3982 4167 4612 325 Size: 1255 680 1552 17 153 185 445 1498 Fragments arranged by size: 1552 1498 1255 680 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4031 5743 96 Size: 274 80 33 51 1443 2054 1712 138 Fragments arranged by size: 2054 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2434 2466 2656 2688 5745 5777 2434 Size: 32 190 32 3057 32 2442 Fragments arranged by size: 3057 2442 190 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5785 XbaI T'CTAG_A Cuts at: 3121 3121 Size: 5785 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5785 XmaI C'CCGG_G Cuts at: 1870 3110 1870 Size: 1240 4545 XmnI GAAnn'nnTTC Cuts at: 3914 3914 Size: 5785 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3593 276 Size: 53 83 186 936 2059 2468 Fragments arranged by size: 2468 2059 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI