HIF3A ORF clone FHC03642

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC03642

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Clone info.

Species

human

Clone Name

pFN21AB6219 Sequence (with vector) Restriction Map

Product ID

FHC03642

Flexi Clone PID

FXC03642

ORF Sequence

DNA sequence (2007 bp)
Protein sequence (669 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB527715]

Gene

KIBB6219

Symbol

HIF3A

Alias : HIF-3A, HIF3-alpha-1, IPAS, MOP7, PASD7, bHLHe17

Description

hypoxia inducible factor 3, alpha subunit, transcript variant 3

Original Clone ID

cs00819

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 2007 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 669 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS12681.2 7.5e-193 100.0 HIF3A CCDS12682.1 8.1e-191 99.7 HIF3A CCDS42580.2 7.6e-159 100.0 HIF3A CCDS58324.1 1e-53 40.1 HIF1A CCDS9754.1 1.5e-53 40.0 HIF1A The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR013767 92 146 PF00989 PAS fold IPR013655 252 337 PF08447 PAS fold-3 IPR021537 478 509 PF11413 Hypoxia-inducible factor HMMSmart IPR001092 20 75 SM00353 Helix-loop-helix DNA-binding domain IPR000014 84 150 SM00091 PAS IPR000014 227 295 SM00091 PAS IPR001610 301 344 SM00086 PAC motif HMMTigr IPR000014 248 324 TIGR00229 PAS ProfileScan IPR000014 90 154 PS50112 PAS IPR000014 246 297 PS50112 PAS
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_690008 669 609976 100.0 100.0 NP_690007 667 609976 99.7 99.3 XP_005259209 713 609976 100.0 98.7 XP_016882627 663 609976 97.3 98.4 XP_016882625 707 609976 97.2 97.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB6219 Download

>KIBB6219  2007 bp
ATGGCGCTGGGGCTGCAGCGCGCAAGGTCGACCACGGAGCTGCGCAAGGAAAAGTCCCGG
GATGCGGCCCGCAGCCGGCGCAGCCAGGAGACCGAGGTGCTGTACCAGCTGGCTCACACG
CTGCCCTTCGCCCGCGGCGTCAGCGCCCACCTGGACAAGGCCTCTATCATGCGCCTCACC
ATCAGCTACCTGCGCATGCACCGCCTCTGCGCCGCAGGGGAGTGGAACCAGGTGGGAGCA
GGGGGAGAACCACTGGATGCCTGCTACCTGAAGGCCCTGGAGGGCTTCGTCATGGTGCTC
ACCGCCGAGGGAGACATGGCTTACCTGTCGGAGAATGTCAGCAAACACCTGGGCCTCAGT
CAGCTGGAGCTCATTGGACACAGCATCTTTGATTTCATCCACCCCTGTGACCAAGAGGAG
CTTCAGGACGCCCTGACCCCCCAGCAGACCCTGTCCAGGAGGAAGGTGGAGGCCCCCACG
GAGCGGTGCTTCTCCTTGCGCATGAAGAGTACGCTCACCAGCCGCGGGCGCACCCTCAAC
CTCAAGGCGGCCACCTGGAAGGTGCTGAACTGCTCTGGACATATGAGGGCCTACAAGCCA
CCTGCGCAGACTTCTCCAGCTGGGAGCCCTGACTCAGAGCCCCCGCTGCAGTGCCTGGTG
CTCATCTGCGAAGCCATCCCCCACCCAGGCAGCCTGGAGCCCCCACTGGGCCGAGGGGCC
TTCCTCAGCCGCCACAGCCTGGACATGAAGTTCACCTACTGTGACGACAGGATTGCAGAA
GTGGCTGGCTATAGTCCCGATGACCTGATCGGCTGTTCCGCCTACGAGTACATCCACGCG
CTGGACTCCGACGCGGTCAGCAAGAGCATCCACACCTTGCTGAGCAAGGGCCAGGCAGTA
ACAGGGCAGTATCGCTTCCTGGCCCGGAGTGGTGGCTACCTGTGGACCCAGACCCAGGCC
ACAGTGGTGTCAGGGGGACGGGGCCCCCAGTCGGAGAGTATCGTCTGTGTCCATTTTTTA
ATCAGCCAGGTGGAAGAGACCGGAGTGGTGCTGTCCCTGGAGCAAACGGAGCAACACTCT
CGCAGACCCATTCAGCGGGGCGCCCCCTCTCAGAAGGACACCCCTAACCCTGGGGACAGC
CTTGACACCCCTGGCCCCCGGATCCTTGCCTTCCTGCACCCGCCTTCCCTGAGCGAGGCT
GCCCTGGCCGCTGACCCCCGCCGTTTCTGCAGCCCTGACCTCCGTCGCCTCCTGGGACCC
ATCCTGGATGGGGCTTCAGTAGCAGCCACTCCCAGCACCCCGCTGGCCACACGGCACCCC
CAAAGTCCTCTTTCGGCTGATCTCCCAGATGAACTACCTGTGGGCACCGAGAATGTGCAC
AGACTCTTCACCTCCGGGAAAGACACTGAGGCAGTGGAGACAGATTTAGATATAGCTCAG
GATGCTGATGCTCTGGATTTGGAGATGCTGGCCCCCTACATCTCCATGGATGATGACTTC
CAGCTCAACGCCAGCGAGCAGCTACCCAGGGCCTACCACAGACCTCTGGGGGCTGTCCCC
CGGCCCCGTGCTCGGAGCTTCCATGGCCTGTCACCTCCAGCCCTTGAGCCCTCCCTGCTA
CCCCGCTGGGGGAGTGACCCCCGGCTGAGCTGCTCCAGCCCTTCCAGAGGGGACCCCTCA
GCATCCTCTCCCATGGCTGGGGCTCGGAAGAGGACCCTGGCCCAGAGCTCAGAGGACGAG
GACGAGGGAGTGGAGCTGCTGGGAGTGAGACCTCCCAAAAGGTCCCCCAGCCCAGAACAC
GAAAACTTTCTGCTCTTTCCTCTCAGCCTGAGTTTCCTTCTGACAGGAGGACCAGCCCCA
GGGAGCCTGCAGGACCCCAGCACCCCACTCCTGAACCTGAATGAGCCCCTGGGCCTGGGC
CCCTCACTGCTCTCTCCGTACTCAGACGAGGACACTACCCAGCCCGGGGGCCCCTTCCAG
CCAAGGGCAGGCTCAGCCCAGGCTGAC

ORF Protein

Cloned ORF protein sequence for pFN21AB6219 Download

>KIBB6219  669 aa
MALGLQRARSTTELRKEKSRDAARSRRSQETEVLYQLAHTLPFARGVSAHLDKASIMRLT
ISYLRMHRLCAAGEWNQVGAGGEPLDACYLKALEGFVMVLTAEGDMAYLSENVSKHLGLS
QLELIGHSIFDFIHPCDQEELQDALTPQQTLSRRKVEAPTERCFSLRMKSTLTSRGRTLN
LKAATWKVLNCSGHMRAYKPPAQTSPAGSPDSEPPLQCLVLICEAIPHPGSLEPPLGRGA
FLSRHSLDMKFTYCDDRIAEVAGYSPDDLIGCSAYEYIHALDSDAVSKSIHTLLSKGQAV
TGQYRFLARSGGYLWTQTQATVVSGGRGPQSESIVCVHFLISQVEETGVVLSLEQTEQHS
RRPIQRGAPSQKDTPNPGDSLDTPGPRILAFLHPPSLSEAALAADPRRFCSPDLRRLLGP
ILDGASVAATPSTPLATRHPQSPLSADLPDELPVGTENVHRLFTSGKDTEAVETDLDIAQ
DADALDLEMLAPYISMDDDFQLNASEQLPRAYHRPLGAVPRPRARSFHGLSPPALEPSLL
PRWGSDPRLSCSSPSRGDPSASSPMAGARKRTLAQSSEDEDEGVELLGVRPPKRSPSPEH
ENFLLFPLSLSFLLTGGPAPGSLQDPSTPLLNLNEPLGLGPSLLSPYSDEDTTQPGGPFQ
PRAGSAQAD

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB6219 Download

>pFN21AB6219  6712 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCGCTGGGGCTGCAGCGCGCAAGGTCGACCAC
GGAGCTGCGCAAGGAAAAGTCCCGGGATGCGGCCCGCAGCCGGCGCAGCCAGGAGACCGA
GGTGCTGTACCAGCTGGCTCACACGCTGCCCTTCGCCCGCGGCGTCAGCGCCCACCTGGA
CAAGGCCTCTATCATGCGCCTCACCATCAGCTACCTGCGCATGCACCGCCTCTGCGCCGC
AGGGGAGTGGAACCAGGTGGGAGCAGGGGGAGAACCACTGGATGCCTGCTACCTGAAGGC
CCTGGAGGGCTTCGTCATGGTGCTCACCGCCGAGGGAGACATGGCTTACCTGTCGGAGAA
TGTCAGCAAACACCTGGGCCTCAGTCAGCTGGAGCTCATTGGACACAGCATCTTTGATTT
CATCCACCCCTGTGACCAAGAGGAGCTTCAGGACGCCCTGACCCCCCAGCAGACCCTGTC
CAGGAGGAAGGTGGAGGCCCCCACGGAGCGGTGCTTCTCCTTGCGCATGAAGAGTACGCT
CACCAGCCGCGGGCGCACCCTCAACCTCAAGGCGGCCACCTGGAAGGTGCTGAACTGCTC
TGGACATATGAGGGCCTACAAGCCACCTGCGCAGACTTCTCCAGCTGGGAGCCCTGACTC
AGAGCCCCCGCTGCAGTGCCTGGTGCTCATCTGCGAAGCCATCCCCCACCCAGGCAGCCT
GGAGCCCCCACTGGGCCGAGGGGCCTTCCTCAGCCGCCACAGCCTGGACATGAAGTTCAC
CTACTGTGACGACAGGATTGCAGAAGTGGCTGGCTATAGTCCCGATGACCTGATCGGCTG
TTCCGCCTACGAGTACATCCACGCGCTGGACTCCGACGCGGTCAGCAAGAGCATCCACAC
CTTGCTGAGCAAGGGCCAGGCAGTAACAGGGCAGTATCGCTTCCTGGCCCGGAGTGGTGG
CTACCTGTGGACCCAGACCCAGGCCACAGTGGTGTCAGGGGGACGGGGCCCCCAGTCGGA
GAGTATCGTCTGTGTCCATTTTTTAATCAGCCAGGTGGAAGAGACCGGAGTGGTGCTGTC
CCTGGAGCAAACGGAGCAACACTCTCGCAGACCCATTCAGCGGGGCGCCCCCTCTCAGAA
GGACACCCCTAACCCTGGGGACAGCCTTGACACCCCTGGCCCCCGGATCCTTGCCTTCCT
GCACCCGCCTTCCCTGAGCGAGGCTGCCCTGGCCGCTGACCCCCGCCGTTTCTGCAGCCC
TGACCTCCGTCGCCTCCTGGGACCCATCCTGGATGGGGCTTCAGTAGCAGCCACTCCCAG
CACCCCGCTGGCCACACGGCACCCCCAAAGTCCTCTTTCGGCTGATCTCCCAGATGAACT
ACCTGTGGGCACCGAGAATGTGCACAGACTCTTCACCTCCGGGAAAGACACTGAGGCAGT
GGAGACAGATTTAGATATAGCTCAGGATGCTGATGCTCTGGATTTGGAGATGCTGGCCCC
CTACATCTCCATGGATGATGACTTCCAGCTCAACGCCAGCGAGCAGCTACCCAGGGCCTA
CCACAGACCTCTGGGGGCTGTCCCCCGGCCCCGTGCTCGGAGCTTCCATGGCCTGTCACC
TCCAGCCCTTGAGCCCTCCCTGCTACCCCGCTGGGGGAGTGACCCCCGGCTGAGCTGCTC
CAGCCCTTCCAGAGGGGACCCCTCAGCATCCTCTCCCATGGCTGGGGCTCGGAAGAGGAC
CCTGGCCCAGAGCTCAGAGGACGAGGACGAGGGAGTGGAGCTGCTGGGAGTGAGACCTCC
CAAAAGGTCCCCCAGCCCAGAACACGAAAACTTTCTGCTCTTTCCTCTCAGCCTGAGTTT
CCTTCTGACAGGAGGACCAGCCCCAGGGAGCCTGCAGGACCCCAGCACCCCACTCCTGAA
CCTGAATGAGCCCCTGGGCCTGGGCCCCTCACTGCTCTCTCCGTACTCAGACGAGGACAC
TACCCAGCCCGGGGGCCCCTTCCAGCCAAGGGCAGGCTCAGCCCAGGCTGACGTTTAAAC
GAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATC
CGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAAC
TAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTT
GGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCT
ATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATT
CATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTC
TACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTC
CAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTC
TACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCT
ATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGA
TAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCC
CTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTG
AAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTC
AACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACT
TTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTC
GGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAG
CATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGAT
AACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTT
TTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAA
GCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGC
AAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATG
GAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATT
GCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA
GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGAT
GAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAA
ATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTAT
CCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCA
GGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGC
ATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACC
AGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCG
GATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTA
GGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCG
TTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGAC
ACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAG
GCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTAT
TTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGAT
CCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGC
GCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGT
GGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCT
AGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGG
TGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCT
CTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCG
GGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCG
GTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAG
ATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT