(Circular) (Six-base) MAPSORT of: pFN21AB6219.seq Check: 2823 from: 1 to: 6712 pFN21AB6219 6712 bp With 182 enzymes: SgfI * August 27, 2010 15:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2614 2614 Size: 6712 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4522 278 Size: 53 83 186 936 2986 2468 Fragments arranged by size: 2986 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2033 4055 1937 Size: 96 2022 4594 Fragments arranged by size: 4594 2022 96 Acc65I G'GTAC_C Cuts at: 4033 4033 Size: 6712 AclI AA'CG_TT Cuts at: 1563 4839 5212 1563 Size: 3276 373 3063 Fragments arranged by size: 3276 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2294 2411 3264 4775 6159 6427 1632 Size: 662 117 853 1511 1384 268 1917 Fragments arranged by size: 1917 1511 1384 853 662 268 117 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6515 AflIII A'CryG_T Cuts at: 1203 5611 6588 1203 Size: 4408 977 1327 Fragments arranged by size: 4408 1327 977 AgeI A'CCGG_T Cuts at: 5543 5543 Size: 6712 AhdI GACnn_n'nnGTC Cuts at: 5441 5441 Size: 6712 AleI CACnn'nnGTG Cuts at: 1166 2409 2969 1166 Size: 1243 560 4909 Fragments arranged by size: 4909 1243 560 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6712 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2738 2770 2738 Size: 32 6680 AlwNI CAG_nnn'CTG Cuts at: 2455 2659 4455 6027 2455 Size: 204 1796 1572 3140 Fragments arranged by size: 3140 1796 1572 204 ApaI G_GGCC'C Cuts at: 2990 3926 3977 2990 Size: 936 51 5725 Fragments arranged by size: 5725 936 51 ApaLI G'TGCA_C Cuts at: 3380 4769 5925 3380 Size: 1389 1156 4167 Fragments arranged by size: 4167 1389 1156 ApoI r'AATT_y Cuts at: 1493 4021 4247 4399 6508 1493 Size: 2528 226 152 2109 1697 Fragments arranged by size: 2528 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5266 160 Size: 872 4234 1606 Fragments arranged by size: 4234 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6712 AvaI C'yCGr_G Cuts at: 1870 1943 2061 3968 4037 1870 Size: 73 118 1907 69 4545 Fragments arranged by size: 4545 1907 118 73 69 BamHI G'GATC_C Cuts at: 3165 4042 4409 3165 Size: 877 367 5468 Fragments arranged by size: 5468 877 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3104 3318 3368 4033 5488 Size: 325 217 627 1317 214 50 665 1455 Cuts at: 5488 618 Size: 1842 Fragments arranged by size: 1842 1455 1317 665 627 325 217 214 50 BanII G_rGCy'C Cuts at: 1391 2376 2633 2646 2706 2990 3615 3709 3734 Size: 985 257 13 60 284 625 94 25 Cuts at: 3734 3912 3926 3977 4031 1391 Size: 178 14 51 54 4072 Fragments arranged by size: 4072 985 625 284 257 178 94 60 54 51 25 14 13 BbeI G_GCGC'C Cuts at: 3108 3108 Size: 6712 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5741 BbvCI CC'TCA_GC Cuts at: 2729 3682 2729 Size: 953 5759 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2759 2793 3065 3099 4903 4937 1903 Size: 34 822 34 272 34 1804 34 3678 Fragments arranged by size: 3678 1804 822 272 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4604 5814 4604 Size: 1210 5502 BclI T'GATC_A Cuts at: 1548 1548 Size: 6712 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5323 136 Size: 107 122 71 631 605 3651 1525 Fragments arranged by size: 3651 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6707 6707 Size: 6712 BlpI GC'TnA_GC Cuts at: 2885 3650 3997 4129 2885 Size: 765 347 132 5468 Fragments arranged by size: 5468 765 347 132 Bme1580I G_kGCm'C Cuts at: 1790 2990 3371 3384 3926 3977 4773 5929 1790 Size: 1200 381 13 542 51 796 1156 2573 Fragments arranged by size: 2573 1200 1156 796 542 381 51 13 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6712 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2720 2986 4443 Size: 416 53 83 399 24 1303 266 1457 Cuts at: 4443 5401 442 Size: 958 1753 Fragments arranged by size: 1753 1457 1303 958 416 399 266 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6712 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3097 3097 Size: 6712 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2303 2390 2604 2720 3083 3585 3643 5372 Size: 1064 87 214 116 363 502 58 1729 Cuts at: 5372 1239 Size: 2579 Fragments arranged by size: 2579 1729 1064 502 363 214 116 87 58 Bpu10I CC'TnA_GC Cuts at: 2729 3194 3441 3682 2729 Size: 465 247 241 5759 Fragments arranged by size: 5759 465 247 241 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2531 3629 4837 5702 6000 2531 Size: 1098 1208 865 298 3243 Fragments arranged by size: 3243 1208 1098 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2087 3035 3766 5375 882 Size: 617 588 948 731 1609 2219 Fragments arranged by size: 2219 1609 948 731 617 588 BsaAI yAC'GTr Cuts at: 493 1204 4503 493 Size: 711 3299 2702 Fragments arranged by size: 3299 2702 711 BsaBI GATnn'nnATC Cuts at: 6706 6706 Size: 6712 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2142 2433 3105 4519 Size: 53 83 186 936 609 291 672 1414 Cuts at: 4519 4901 5536 275 Size: 382 635 1451 Fragments arranged by size: 1451 1414 936 672 635 609 382 291 186 83 53 BsaWI w'CCGG_w Cuts at: 3044 5145 5543 5817 5964 6375 3044 Size: 2101 398 274 147 411 3381 Fragments arranged by size: 3381 2101 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6487 Fragments arranged by size: 6487 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2435 1186 Size: 1249 5463 BseYI C'CCAG_C Cuts at: 2011 2445 2624 3296 3630 3701 3763 3791 3881 Size: 434 179 672 334 71 62 28 90 Cuts at: 3881 3963 5915 6411 2011 Size: 82 1952 496 2312 Fragments arranged by size: 2312 1952 672 496 434 334 179 90 82 71 62 28 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3164 1163 Size: 573 1428 4711 Fragments arranged by size: 4711 1428 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4162 4923 5951 1540 Size: 275 186 2161 761 1028 2301 Fragments arranged by size: 2301 2161 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2304 2376 2667 3384 3577 3734 4773 4858 5929 Size: 72 291 717 193 157 1039 85 1071 Cuts at: 5929 2304 Size: 3087 Fragments arranged by size: 3087 1071 1039 717 291 193 157 85 72 BsmI GAATG_Cn' Cuts at: 4223 4316 4223 Size: 93 6619 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2304 2376 2633 2646 2667 2706 2990 Size: 399 514 72 257 13 21 39 284 Cuts at: 2990 3371 3384 3577 3615 3709 3734 3912 3926 Size: 381 13 193 38 94 25 178 14 Cuts at: 3926 3977 4031 4773 4858 5929 1391 Size: 51 54 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 514 399 381 284 257 193 178 94 85 72 54 51 39 38 25 21 14 13 13 BspEI T'CCGG_A Cuts at: 6375 6375 Size: 6712 BspHI T'CATG_A Cuts at: 4601 6331 4601 Size: 1730 4982 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2202 2614 4067 844 Size: 1358 412 1453 3489 Fragments arranged by size: 3489 1453 1358 412 BsrBI CCG'CTC Cuts at: 1394 1823 2488 4599 1394 Size: 429 665 2111 3507 Fragments arranged by size: 3507 2111 665 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5207 5381 65 Size: 1183 3959 174 1396 Fragments arranged by size: 3959 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2079 5356 5543 1700 Size: 110 269 3277 187 2869 Fragments arranged by size: 3277 2869 269 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6712 BssHII G'CGCG_C Cuts at: 1925 2023 1925 Size: 98 6614 BssSI C'ACGA_G Cuts at: 4772 5784 4772 Size: 1012 5700 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6712 BstBI TT'CG_AA Cuts at: 5516 5516 Size: 6712 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6712 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2011 Size: 6712 BstYI r'GATC_y Cuts at: 1972 3165 4042 4409 4794 4811 6263 6349 6361 Size: 1193 877 367 385 17 1452 86 12 Cuts at: 6361 6707 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1193 877 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2037 2137 2481 2527 3489 3586 Size: 552 939 33 100 344 46 962 97 Cuts at: 3586 3696 513 Size: 110 3529 Fragments arranged by size: 3529 962 939 552 344 110 100 97 46 33 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5973 Fragments arranged by size: 5973 683 56 BtsI GCAGTG_nn' Cuts at: 2660 3423 3929 4228 5022 5042 2660 Size: 763 506 299 794 20 4330 Fragments arranged by size: 4330 794 763 506 299 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6712 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6677 DraI TTT'AAA Cuts at: 4016 4016 Size: 6712 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5719 809 Size: 507 4403 1802 Fragments arranged by size: 4403 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2553 3210 3309 Size: 54 1425 50 275 18 723 657 99 Cuts at: 3309 4159 5048 6677 8 Size: 850 889 1629 43 Fragments arranged by size: 1629 1425 889 850 723 657 275 99 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4159 1537 Size: 275 2347 4090 Fragments arranged by size: 4090 2347 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2504 3033 3395 3707 4642 1353 Size: 1151 529 362 312 935 3423 Fragments arranged by size: 3423 1151 935 529 362 312 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2812 5299 5673 5819 387 Size: 1064 138 1223 2487 374 146 1280 Fragments arranged by size: 2487 1280 1223 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2374 3732 2374 Size: 1358 5354 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2294 2303 2390 2411 2604 2720 3083 Size: 393 662 9 87 21 193 116 363 Cuts at: 3083 3264 3585 3643 4775 5372 6159 6427 1239 Size: 181 321 58 1132 597 787 268 1524 Fragments arranged by size: 1524 1132 787 662 597 393 363 321 268 193 181 116 87 58 21 9 EcoNI CCTnn'n_nnAGG Cuts at: 2159 3197 3669 2159 Size: 1038 472 5202 Fragments arranged by size: 5202 1038 472 EcoO109I rG'GnC_Cy Cuts at: 2278 2476 2592 2721 2986 2987 3260 3534 3676 Size: 198 116 129 265 1 273 274 142 Cuts at: 3676 3717 3786 3877 3923 3973 3974 2278 Size: 41 69 91 46 50 1 5016 Fragments arranged by size: 5016 274 273 265 198 142 129 116 91 69 50 46 41 1 1 EcoRI G'AATT_C Cuts at: 4021 4399 4021 Size: 378 6334 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2441 2441 Size: 6712 FspI TGC'GCA Cuts at: 2048 2198 2504 2610 5218 2048 Size: 150 306 106 2608 3542 Fragments arranged by size: 3542 2608 306 150 106 HaeII r_GCGC'y Cuts at: 1678 2012 2151 3108 5859 1678 Size: 334 139 957 2751 2531 Fragments arranged by size: 2751 2531 957 334 139 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2150 2182 2373 2405 5353 Size: 32 135 32 578 32 191 32 2948 Cuts at: 5353 5385 5427 5459 1373 Size: 32 42 32 2626 Fragments arranged by size: 2948 2626 578 191 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2034 4056 4302 669 Size: 988 281 96 2022 246 3079 Fragments arranged by size: 3079 2022 988 281 246 96 HindIII A'AGCT_T Cuts at: 748 748 Size: 6712 HpaI GTT'AAC Cuts at: 4302 4302 Size: 6712 KasI G'GCGC_C Cuts at: 3104 3104 Size: 6712 KpnI G_GTAC'C Cuts at: 4037 4037 Size: 6712 MfeI C'AATT_G Cuts at: 4311 4311 Size: 6712 MluI A'CGCG_T Cuts at: 6588 6588 Size: 6712 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2313 2877 2976 5826 6010 1305 Size: 1008 564 99 2850 184 2007 Fragments arranged by size: 2850 2007 1008 564 184 99 MscI TGG'CCA Cuts at: 10 64 1489 1832 3311 10 Size: 54 1425 343 1479 3411 Fragments arranged by size: 3411 1479 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2409 2969 3488 4670 5029 5188 518 Size: 648 1243 560 519 1182 359 159 2042 Fragments arranged by size: 2042 1243 1182 648 560 519 359 159 MspA1I CmG'CkG Cuts at: 1606 2113 2139 2368 2529 2624 2650 3100 3215 Size: 507 26 229 161 95 26 450 115 Cuts at: 3215 3307 3630 4128 4805 5953 6198 1606 Size: 92 323 498 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 507 498 450 323 245 229 161 115 95 92 26 26 NaeI GCC'GGC Cuts at: 2081 2081 Size: 6712 NarI GG'CG_CC Cuts at: 3105 3105 Size: 6712 NcoI C'CATG_G Cuts at: 513 1065 2004 3489 3586 3696 513 Size: 552 939 1485 97 110 3529 Fragments arranged by size: 3529 1485 939 552 110 97 NdeI CA'TA_TG Cuts at: 387 2586 387 Size: 2199 4513 NgoMIV G'CCGG_C Cuts at: 2079 2079 Size: 6712 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6712 NotI GC'GGCC_GC Cuts at: 4159 4159 Size: 6712 NspI r_CATG'y Cuts at: 1132 1229 2203 4070 5615 1132 Size: 97 974 1867 1545 2229 Fragments arranged by size: 2229 1867 1545 974 97 PasI CC'CwG_GG Cuts at: 1348 3134 3531 3863 3913 1348 Size: 1786 397 332 50 4147 Fragments arranged by size: 4147 1786 397 332 50 PciI A'CATG_T Cuts at: 5611 5611 Size: 6712 PflMI CCAn_nnn'nTGG Cuts at: 1108 2743 2845 1108 Size: 1635 102 4975 Fragments arranged by size: 4975 1635 102 PfoI T'CCnGG_A Cuts at: 2459 3267 3398 2459 Size: 808 131 5773 Fragments arranged by size: 5773 808 131 PmeI GTTT'AAAC Cuts at: 4016 4016 Size: 6712 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4781 4813 6294 6326 4781 Size: 32 1481 32 5167 Fragments arranged by size: 5167 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3260 3676 3717 3786 3877 3260 Size: 416 41 69 91 6095 Fragments arranged by size: 6095 416 91 69 41 PshAI GACnn'nnGTC Cuts at: 1613 3246 1613 Size: 1633 5079 PsiI TTA'TAA Cuts at: 4282 4282 Size: 6712 PspOMI G'GGCC_C Cuts at: 2986 3922 3973 2986 Size: 936 51 5725 Fragments arranged by size: 5725 936 51 PstI C_TGCA'G Cuts at: 830 2022 2655 3236 3876 4064 830 Size: 1192 633 581 640 188 3478 Fragments arranged by size: 3478 1192 640 633 581 188 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6712 PvuII CAG'CTG Cuts at: 2113 2368 2624 2113 Size: 255 256 6201 Fragments arranged by size: 6201 256 255 SacI G_AGCT'C Cuts at: 2376 3734 2376 Size: 1358 5354 SacII CC_GC'GG Cuts at: 2140 2530 2140 Size: 390 6322 SalI G'TCGA_C Cuts at: 1936 2032 4054 1936 Size: 96 2022 4594 Fragments arranged by size: 4594 2022 96 SanDI GG'GwC_CC Cuts at: 3260 3676 3260 Size: 416 6296 SbfI CC_TGCA'GG Cuts at: 3876 4064 3876 Size: 188 6524 ScaI AGT'ACT Cuts at: 4960 6672 4960 Size: 1712 5000 SexAI A'CCwGG_T Cuts at: 2232 2232 Size: 6712 SfcI C'TryA_G Cuts at: 826 1045 2018 2651 2794 3232 3872 4060 5195 Size: 219 973 633 143 438 640 188 1135 Cuts at: 5195 5876 6067 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 973 681 640 633 438 219 191 188 143 SfoI GGC'GCC Cuts at: 3106 3106 Size: 6712 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6712 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6712 SmaI CCC'GGG Cuts at: 1872 2063 3970 4039 1872 Size: 191 1907 69 4545 Fragments arranged by size: 4545 1907 191 69 SmlI C'TyrA_G Cuts at: 820 1017 1943 2546 3608 4816 5717 5979 820 Size: 197 926 603 1062 1208 901 262 1553 Fragments arranged by size: 1553 1208 1062 926 901 603 262 197 SnaBI TAC'GTA Cuts at: 493 4503 493 Size: 4010 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6712 SphI G_CATG'C Cuts at: 1132 2203 4070 1132 Size: 1071 1867 3774 Fragments arranged by size: 3774 1867 1071 SspI AAT'ATT Cuts at: 5 52 4636 5 Size: 47 4584 2081 Fragments arranged by size: 4584 2081 47 StuI AGG'CCT Cuts at: 1517 2165 1517 Size: 648 6064 StyI C'CwwG_G Cuts at: 513 1065 2004 3489 3586 3696 3986 4151 513 Size: 552 939 1485 97 110 290 165 3074 Fragments arranged by size: 3074 1485 939 552 290 165 110 97 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2111 2703 4739 4756 4909 5094 5539 Size: 1255 531 592 2036 17 153 185 445 Cuts at: 5539 325 Size: 1498 Fragments arranged by size: 2036 1498 1255 592 531 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2832 4958 6670 Size: 274 80 33 51 1443 855 2126 1712 Cuts at: 6670 96 Size: 138 Fragments arranged by size: 2126 1712 1443 855 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3173 3205 6672 6704 3173 Size: 32 3467 32 3181 Fragments arranged by size: 3467 3181 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2455 2858 1316 Size: 1139 403 5170 Fragments arranged by size: 5170 1139 403 XbaI T'CTAG_A Cuts at: 4048 4048 Size: 6712 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2467 3304 2467 Size: 837 5875 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6712 XmaI C'CCGG_G Cuts at: 1870 2061 3968 4037 1870 Size: 191 1907 69 4545 Fragments arranged by size: 4545 1907 191 69 XmnI GAAnn'nnTTC Cuts at: 3810 4841 3810 Size: 1031 5681 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4520 276 Size: 53 83 186 936 2986 2468 Fragments arranged by size: 2986 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FseI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SapI SfiI SrfI SwaI