APLP2 ORF clone FHC02217

KOP
KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC02217

How to Order Order Information

Clone info.

Species

human

Clone Name

pFN21AB5688 Sequence (with vector) Restriction Map

Product ID

FHC02217

Flexi Clone PID

FXC02217

Original Clone PID

ORK02217

ORF Sequence

DNA sequence (2085 bp)
Protein sequence (695 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB462943]

Gene

KIBB5688

Symbol

APLP2

Alias : APLP-2, APPH, APPL2, CDEBP

Description

amyloid beta (A4) precursor-like protein 2, transcript variant 3

Original Clone ID

ek00172

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 2085 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 695 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS44774.1 1.8e-168 100.0 APLP2 CCDS44775.1 1.6e-89 75.1 APLP2 CCDS44773.1 2.1e-89 92.2 APLP2 CCDS58196.1 2.1e-89 92.0 APLP2 CCDS8486.1 1.4e-73 90.5 APLP2 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR008155 193 211 PR00203 Amyloidogenic glycoprotein IPR008155 325 348 PR00203 Amyloidogenic glycoprotein IPR008155 624 649 PR00203 Amyloidogenic glycoprotein IPR008155 670 692 PR00203 Amyloidogenic glycoprotein HMMPfam IPR008154 44 204 PF02177 Amyloidogenic glycoprotein IPR019543 639 691 PF10515 Beta-amyloid precursor protein C-terminal HMMSmart IPR008154 42 204 SM00006 Amyloidogenic glycoprotein
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 10 AATGRLLLLLLVGLTAPALALAG 32 PRIMARY 23 2 624 SSALIGLLVIAVAIATVIVISLV 646 PRIMARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_001135749 695 104776 100.0 100.0 NP_001315611 707 104776 98.3 100.0 NP_001315614 644 104776 92.7 100.0 NP_001315613 700 104776 85.6 100.0 NP_001135750 522 104776 75.1 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB5688 Download

>KIBB5688  2085 bp
ATGGCGGCCACCGGGACCGCGGCCGCCGCAGCCACGGGCAGGCTCCTGCTTCTGCTGCTG
GTGGGGCTCACGGCGCCTGCCTTGGCGCTGGCCGGCTACATCGAGGCTCTTGCAGCCAAT
GCCGGAACAGGATTTGCTGTTGCTGAGCCTCAAATCGCAATGTTTTGTGGGAAGTTAAAT
ATGCATGTGAACATTCAGACTGGGAAATGGGAACCTGATCCAACAGGCACCAAGAGCTGC
TTTGAAACAAAAGAAGAAGTTCTTCAGTACTGTCAGGAGATGTATCCAGAGCTACAGATC
ACAAATGTGATGGAGGCAAACCAGCGGGTTAGTATTGACAACTGGTGCCGGAGGGACAAA
AAGCAATGCAAGAGTCGCTTTGTTACACCTTTCAAGTGTCTCGTGGGTGAATTTGTAAGT
GATGTCCTGCTAGTTCCAGAAAAGTGCCAGTTTTTCCACAAAGAGCGGATGGAGGTGTGT
GAGAATCACCAGCACTGGCACACGGTAGTCAAAGAGGCATGTCTGACTCAGGGAATGACC
TTATATAGCTACGGCATGCTGCTCCCATGTGGGGTAGACCAGTTCCATGGCACTGAATAT
GTGTGCTGCCCTCAGACAAAGATTATTGGATCTGTGTCAAAAGAAGAGGAAGAGGAAGAT
GAAGAGGAAGAGGAAGAGGAAGATGAAGAGGAAGACTATGATGTTTATAAAAGTGAATTT
CCTACTGAAGCAGATCTGGAAGACTTCACAGAAGCAGCTGTGGATGAGGATGATGAGGAT
GAGGAAGAAGGGGAGGAAGTGGTGGAGGACCGAGATTACTACTATGACACCTTCAAAGGA
GATGACTACAATGAGGAGAATCCTACTGAACCCGGCAGCGACGGCACCATGTCAGACAAG
GAAATTACTCATGATGTCAAAGTTCCTCCAACTCCTCTGCCAACCAATGATGTTGATGTG
TATTTCGAGACCTCTGCAGATGATAATGAGCATGCTCGCTTCCAGAAGGCTAAGGAGCAG
CTGGAGATTCGGCACCGCAACCGAATGGACAGGGTAAAGAAGGAATGGGAAGAGGCAGAG
CTTCAAGCTAAGAACCTCCCCAAAGCAGAGAGGCAGACTCTGATTCAGCACTTCCAAGCC
ATGGTTAAAGCTTTAGAGAAGGAAGCAGCCAGTGAGAAGCAGCAGCTGGTGGAGACCCAC
CTGGCCCGAGTGGAAGCTATGCTGAATGACCGCCGTCGGATGGCTCTGGAGAACTACCTG
GCTGCCTTGCAGTCTGACCCGCCACGGCCTCATCGCATTCTCCAGGCCTTACGGCGTTAT
GTCCGTGCTGAGAACAAAGATCGCTTACATACCATCCGTCATTACCAGCATGTGTTGGCT
GTTGACCCAGAAAAGGCGGCCCAGATGAAATCCCAGGTGATGACACATCTCCACGTGATT
GAAGAAAGGAGGAACCAAAGCCTCTCTCTGCTCTACAAAGTACCTTATGTAGCCCAAGAA
ATTCAAGAGGAAATTGATGAGCTCCTTCAGGAGCAGCGTGCAGATATGGACCAGTTCACT
GCCTCAATCTCAGAGACCCCTGTGGACGTCCGGGTGAGCTCTGAGGAGAGTGAGGAGATC
CCACCGTTCCACCCCTTCCACCCCTTCCCAGCCCTACCTGAGAACGAAGGATCTGGAGTG
GGAGAGCAGGATGGGGGACTGATCGGTGCCGAAGAGAAAGTGATTAACAGTAAGAATAAA
GTGGATGAAAACATGGTCATTGACGAGACTCTGGATGTTAAGGAAATGATTTTCAATGCC
GAGAGAGTTGGAGGCCTCGAGGAAGAGCGGGAATCCGTGGGCCCACTGCGGGAGGACTTC
AGTCTGAGTAGCAGTGCTCTCATTGGCCTGCTGGTCATCGCAGTGGCCATTGCCACGGTC
ATCGTCATCAGCCTGGTGATGCTGAGGAAGAGGCAGTATGGCACCATCAGCCACGGGATC
GTGGAGGTTGATCCAATGCTCACCCCAGAAGAGCGTCACCTGAACAAGATGCAGAACCAT
GGCTATGAGAACCCCACCTACAAATACCTGGAGCAGATGCAGATT

ORF Protein

Cloned ORF protein sequence for pFN21AB5688 Download

>KIBB5688  695 aa
MAATGTAAAAATGRLLLLLLVGLTAPALALAGYIEALAANAGTGFAVAEPQIAMFCGKLN
MHVNIQTGKWEPDPTGTKSCFETKEEVLQYCQEMYPELQITNVMEANQRVSIDNWCRRDK
KQCKSRFVTPFKCLVGEFVSDVLLVPEKCQFFHKERMEVCENHQHWHTVVKEACLTQGMT
LYSYGMLLPCGVDQFHGTEYVCCPQTKIIGSVSKEEEEEDEEEEEEEDEEEDYDVYKSEF
PTEADLEDFTEAAVDEDDEDEEEGEEVVEDRDYYYDTFKGDDYNEENPTEPGSDGTMSDK
EITHDVKVPPTPLPTNDVDVYFETSADDNEHARFQKAKEQLEIRHRNRMDRVKKEWEEAE
LQAKNLPKAERQTLIQHFQAMVKALEKEAASEKQQLVETHLARVEAMLNDRRRMALENYL
AALQSDPPRPHRILQALRRYVRAENKDRLHTIRHYQHVLAVDPEKAAQMKSQVMTHLHVI
EERRNQSLSLLYKVPYVAQEIQEEIDELLQEQRADMDQFTASISETPVDVRVSSEESEEI
PPFHPFHPFPALPENEGSGVGEQDGGLIGAEEKVINSKNKVDENMVIDETLDVKEMIFNA
ERVGGLEEERESVGPLREDFSLSSSALIGLLVIAVAIATVIVISLVMLRKRQYGTISHGI
VEVDPMLTPEERHLNKMQNHGYENPTYKYLEQMQI

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB5688 Download

>pFN21AB5688  6790 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCGGCCACCGGGACCGCGGCCGCCGCAGCCAC
GGGCAGGCTCCTGCTTCTGCTGCTGGTGGGGCTCACGGCGCCTGCCTTGGCGCTGGCCGG
CTACATCGAGGCTCTTGCAGCCAATGCCGGAACAGGATTTGCTGTTGCTGAGCCTCAAAT
CGCAATGTTTTGTGGGAAGTTAAATATGCATGTGAACATTCAGACTGGGAAATGGGAACC
TGATCCAACAGGCACCAAGAGCTGCTTTGAAACAAAAGAAGAAGTTCTTCAGTACTGTCA
GGAGATGTATCCAGAGCTACAGATCACAAATGTGATGGAGGCAAACCAGCGGGTTAGTAT
TGACAACTGGTGCCGGAGGGACAAAAAGCAATGCAAGAGTCGCTTTGTTACACCTTTCAA
GTGTCTCGTGGGTGAATTTGTAAGTGATGTCCTGCTAGTTCCAGAAAAGTGCCAGTTTTT
CCACAAAGAGCGGATGGAGGTGTGTGAGAATCACCAGCACTGGCACACGGTAGTCAAAGA
GGCATGTCTGACTCAGGGAATGACCTTATATAGCTACGGCATGCTGCTCCCATGTGGGGT
AGACCAGTTCCATGGCACTGAATATGTGTGCTGCCCTCAGACAAAGATTATTGGATCTGT
GTCAAAAGAAGAGGAAGAGGAAGATGAAGAGGAAGAGGAAGAGGAAGATGAAGAGGAAGA
CTATGATGTTTATAAAAGTGAATTTCCTACTGAAGCAGATCTGGAAGACTTCACAGAAGC
AGCTGTGGATGAGGATGATGAGGATGAGGAAGAAGGGGAGGAAGTGGTGGAGGACCGAGA
TTACTACTATGACACCTTCAAAGGAGATGACTACAATGAGGAGAATCCTACTGAACCCGG
CAGCGACGGCACCATGTCAGACAAGGAAATTACTCATGATGTCAAAGTTCCTCCAACTCC
TCTGCCAACCAATGATGTTGATGTGTATTTCGAGACCTCTGCAGATGATAATGAGCATGC
TCGCTTCCAGAAGGCTAAGGAGCAGCTGGAGATTCGGCACCGCAACCGAATGGACAGGGT
AAAGAAGGAATGGGAAGAGGCAGAGCTTCAAGCTAAGAACCTCCCCAAAGCAGAGAGGCA
GACTCTGATTCAGCACTTCCAAGCCATGGTTAAAGCTTTAGAGAAGGAAGCAGCCAGTGA
GAAGCAGCAGCTGGTGGAGACCCACCTGGCCCGAGTGGAAGCTATGCTGAATGACCGCCG
TCGGATGGCTCTGGAGAACTACCTGGCTGCCTTGCAGTCTGACCCGCCACGGCCTCATCG
CATTCTCCAGGCCTTACGGCGTTATGTCCGTGCTGAGAACAAAGATCGCTTACATACCAT
CCGTCATTACCAGCATGTGTTGGCTGTTGACCCAGAAAAGGCGGCCCAGATGAAATCCCA
GGTGATGACACATCTCCACGTGATTGAAGAAAGGAGGAACCAAAGCCTCTCTCTGCTCTA
CAAAGTACCTTATGTAGCCCAAGAAATTCAAGAGGAAATTGATGAGCTCCTTCAGGAGCA
GCGTGCAGATATGGACCAGTTCACTGCCTCAATCTCAGAGACCCCTGTGGACGTCCGGGT
GAGCTCTGAGGAGAGTGAGGAGATCCCACCGTTCCACCCCTTCCACCCCTTCCCAGCCCT
ACCTGAGAACGAAGGATCTGGAGTGGGAGAGCAGGATGGGGGACTGATCGGTGCCGAAGA
GAAAGTGATTAACAGTAAGAATAAAGTGGATGAAAACATGGTCATTGACGAGACTCTGGA
TGTTAAGGAAATGATTTTCAATGCCGAGAGAGTTGGAGGCCTCGAGGAAGAGCGGGAATC
CGTGGGCCCACTGCGGGAGGACTTCAGTCTGAGTAGCAGTGCTCTCATTGGCCTGCTGGT
CATCGCAGTGGCCATTGCCACGGTCATCGTCATCAGCCTGGTGATGCTGAGGAAGAGGCA
GTATGGCACCATCAGCCACGGGATCGTGGAGGTTGATCCAATGCTCACCCCAGAAGAGCG
TCACCTGAACAAGATGCAGAACCATGGCTATGAGAACCCCACCTACAAATACCTGGAGCA
GATGCAGATTGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTG
CAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTG
CCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATA
AGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATT
TGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTT
AACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTT
TTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCG
CTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGT
TTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGA
AATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTC
ATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATT
CAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCT
CACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGT
TACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGT
TTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGAC
GCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTAC
TCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCT
GCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCG
AAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGG
GAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCA
ATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAA
CAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTT
CCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATC
ATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGG
AGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATT
AAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAA
AGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAA
AAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGC
TCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGA
CAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTC
CGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTT
CTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCT
GTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTG
AGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTA
GCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCT
ACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAA
GAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTT
GCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTA
CGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTAT
CAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGAT
GGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGG
TAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGC
GATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGC
GCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCT
GCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGG
ATCGAGATCT