(Circular) (Six-base) MAPSORT of: pFN21AB5688.seq Check: 7826 from: 1 to: 6790 pFN21AB5688 6790 bp With 182 enzymes: SgfI * October 20, 2009 10:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3594 4600 278 Size: 53 83 186 936 2058 1006 2468 Fragments arranged by size: 2468 2058 1006 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2580 4133 1937 Size: 643 1553 4594 Fragments arranged by size: 4594 1553 643 Acc65I G'GTAC_C Cuts at: 4111 4111 Size: 6790 AclI AA'CG_TT Cuts at: 1563 4917 5290 1563 Size: 3354 373 3063 Fragments arranged by size: 3354 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2252 2751 3515 3847 4853 6237 6505 1632 Size: 620 499 764 332 1006 1384 268 1917 Fragments arranged by size: 1917 1384 1006 764 620 499 332 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6593 AflIII A'CryG_T Cuts at: 1203 5689 6666 1203 Size: 4486 977 1327 Fragments arranged by size: 4486 1327 977 AgeI A'CCGG_T Cuts at: 5621 5621 Size: 6790 AhdI GACnn_n'nnGTC Cuts at: 5519 5519 Size: 6790 AleI CACnn'nnGTG Cuts at: 1166 2309 1166 Size: 1143 5647 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2066 1184 Size: 882 5908 AlwNI CAG_nnn'CTG Cuts at: 3124 3190 4533 6105 3124 Size: 66 1343 1572 3809 Fragments arranged by size: 3809 1572 1343 66 ApaI G_GGCC'C Cuts at: 3848 3848 Size: 6790 ApaLI G'TGCA_C Cuts at: 4847 6003 4847 Size: 1156 5634 ApoI r'AATT_y Cuts at: 1493 2414 2720 3504 4099 4325 4477 6586 1493 Size: 921 306 784 595 226 152 2109 1697 Fragments arranged by size: 2109 1697 921 784 595 306 226 152 AseI AT'TA_AT Cuts at: 160 1032 5344 160 Size: 872 4312 1606 Fragments arranged by size: 4312 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6790 AvaI C'yCGr_G Cuts at: 1870 1943 3210 3821 4115 1870 Size: 73 1267 611 294 4545 Fragments arranged by size: 4545 1267 611 294 73 BamHI G'GATC_C Cuts at: 4120 4487 4120 Size: 367 6423 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2077 2231 2349 2888 3036 Size: 325 217 627 290 154 118 539 148 Cuts at: 3036 3710 3965 4111 5566 618 Size: 674 255 146 1455 1842 Fragments arranged by size: 1842 1455 674 627 539 325 290 255 217 154 148 146 118 BanII G_rGCy'C Cuts at: 1391 2073 3528 3605 3848 4109 1391 Size: 682 1455 77 243 261 4072 Fragments arranged by size: 4072 1455 682 261 243 77 BbeI G_GCGC'C Cuts at: 2081 2081 Size: 6790 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2703 2751 928 Size: 971 804 48 4967 Fragments arranged by size: 4967 971 804 48 BbvCI CC'TCA_GC Cuts at: 3947 3947 Size: 6790 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2096 2130 2960 2994 4981 5015 1903 Size: 34 159 34 830 34 1987 34 3678 Fragments arranged by size: 3678 1987 830 159 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2298 4682 5892 2298 Size: 2384 1210 3196 Fragments arranged by size: 3196 2384 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6790 BfrBI ATG'CAT Cuts at: 2188 2188 Size: 6790 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 5401 136 Size: 107 122 71 631 605 337 3392 1525 Fragments arranged by size: 3392 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 2737 6785 2737 Size: 4048 2742 BlpI GC'TnA_GC Cuts at: 2148 4207 2148 Size: 2059 4731 Bme1580I G_kGCm'C Cuts at: 1790 3848 4851 6007 1790 Size: 2058 1003 1156 2573 Fragments arranged by size: 2573 2058 1156 1003 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6790 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2214 4521 5479 Size: 416 53 83 399 24 797 2307 958 Cuts at: 5479 442 Size: 1753 Fragments arranged by size: 2307 1753 958 797 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6790 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3138 3138 Size: 6790 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3047 3272 3290 3699 4094 5450 1239 Size: 1808 225 18 409 395 1356 2579 Fragments arranged by size: 2579 1808 1356 409 395 225 18 Bpu10I CC'TnA_GC Cuts at: 3015 3947 3015 Size: 932 5858 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4915 5780 6078 4915 Size: 865 298 5627 Fragments arranged by size: 5627 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2966 3191 3572 5453 882 Size: 617 1467 225 381 1881 2219 Fragments arranged by size: 2219 1881 1467 617 381 225 BsaAI yAC'GTr Cuts at: 493 1204 3439 4581 493 Size: 711 2235 1142 2702 Fragments arranged by size: 2702 2235 1142 711 BsaBI GATnn'nnATC Cuts at: 6784 6784 Size: 6790 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2078 3591 4597 4979 Size: 53 83 186 936 545 1513 1006 382 Cuts at: 4979 5614 275 Size: 635 1451 Fragments arranged by size: 1513 1451 1006 936 635 545 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5223 5621 5895 6042 6453 5223 Size: 398 274 147 411 5560 Fragments arranged by size: 5560 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6565 Fragments arranged by size: 6565 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2873 2928 3623 3632 1186 Size: 1687 55 695 9 4344 Fragments arranged by size: 4344 1687 695 55 9 BseYI C'CCAG_C Cuts at: 3652 5993 6489 3652 Size: 2341 496 3953 Fragments arranged by size: 3953 2341 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3564 1163 Size: 573 1828 4389 Fragments arranged by size: 4389 1828 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2028 4240 5001 6029 1540 Size: 275 186 27 2212 761 1028 2301 Fragments arranged by size: 2301 2212 1028 761 275 186 27 BsiHKAI G_wGCw'C Cuts at: 3528 3605 3883 4851 4936 6007 3528 Size: 77 278 968 85 1071 4311 Fragments arranged by size: 4311 1071 968 278 85 77 BsmI GAATG_Cn' Cuts at: 3300 4301 4394 3300 Size: 1001 93 5696 Fragments arranged by size: 5696 1001 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2073 3528 3605 3848 3883 4109 4851 Size: 399 283 1455 77 243 35 226 742 Cuts at: 4851 4936 6007 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1455 1071 742 399 283 243 226 85 77 35 BspEI T'CCGG_A Cuts at: 6453 6453 Size: 6790 BspHI T'CATG_A Cuts at: 2914 4679 6409 2914 Size: 1765 1730 3295 Fragments arranged by size: 3295 1765 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4145 844 Size: 3301 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2470 3832 4677 1394 Size: 429 647 1362 845 3507 Fragments arranged by size: 3507 1362 845 647 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2169 2375 3912 5285 5459 65 Size: 1183 921 206 1537 1373 174 1396 Fragments arranged by size: 1537 1396 1373 1183 921 206 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2096 5434 5621 1700 Size: 110 286 3338 187 2869 Fragments arranged by size: 3338 2869 286 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6790 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6790 BssSI C'ACGA_G Cuts at: 2405 4850 5862 2405 Size: 2445 1012 3333 Fragments arranged by size: 3333 2445 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2123 794 Size: 1329 5461 BstBI TT'CG_AA Cuts at: 5594 5594 Size: 6790 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6790 BstXI CCAn_nnnn'nTGG Cuts at: 2591 2591 Size: 6790 BstYI r'GATC_y Cuts at: 1972 2633 2737 3621 3674 4120 4487 4872 4889 Size: 661 104 884 53 446 367 385 17 Cuts at: 4889 6341 6427 6439 6785 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 884 661 446 385 367 346 104 86 53 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2022 2037 2590 3144 3287 3840 Size: 552 939 18 15 553 554 143 553 Cuts at: 3840 3918 3976 4042 513 Size: 78 58 66 3261 Fragments arranged by size: 3261 939 554 553 553 552 143 78 66 58 18 15 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3281 3886 487 Size: 683 56 2055 605 3391 Fragments arranged by size: 3391 2055 683 605 56 BtsI GCAGTG_nn' Cuts at: 3561 3848 3883 3912 4306 5100 5120 3561 Size: 287 35 29 394 794 20 5231 Fragments arranged by size: 5231 794 394 287 35 29 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6790 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6755 DraI TTT'AAA Cuts at: 4094 4094 Size: 6790 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5797 809 Size: 507 4481 1802 Fragments arranged by size: 4481 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2010 2025 2094 Size: 54 1425 50 275 18 180 15 69 Cuts at: 2094 3909 4237 5126 6755 8 Size: 1815 328 889 1629 43 Fragments arranged by size: 1815 1629 1425 889 328 275 180 69 54 50 43 18 15 EagI C'GGCC_G Cuts at: 1537 1812 2025 4237 1537 Size: 275 213 2212 4090 Fragments arranged by size: 4090 2212 275 213 EarI CTCTTCn'nnn_ Cuts at: 1353 2643 2649 2661 2667 2673 2685 3069 3711 Size: 1290 6 12 6 6 12 384 642 Cuts at: 3711 3822 3947 4008 4720 1353 Size: 111 125 61 712 3423 Fragments arranged by size: 3423 1290 712 642 384 125 111 61 12 12 6 6 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5377 5751 5897 387 Size: 1064 138 3788 374 146 1280 Fragments arranged by size: 3788 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3526 3603 3526 Size: 77 6713 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2252 2751 3047 3272 3290 3515 3699 Size: 393 620 499 296 225 18 225 184 Cuts at: 3699 3847 4094 4853 5450 6237 6505 1239 Size: 148 247 759 597 787 268 1524 Fragments arranged by size: 1524 787 759 620 597 499 393 296 268 247 225 225 184 148 18 EcoRI G'AATT_C Cuts at: 4099 4477 4099 Size: 378 6412 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2260 2282 2398 2260 Size: 22 116 6652 Fragments arranged by size: 6652 116 22 FspI TGC'GCA Cuts at: 5296 5296 Size: 6790 HaeII r_GCGC'y Cuts at: 1678 2081 2093 5937 1678 Size: 403 12 3844 2531 Fragments arranged by size: 3844 2531 403 12 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3415 3447 3821 3853 5431 Size: 32 135 32 1843 32 374 32 1578 Cuts at: 5431 5463 5505 5537 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1843 1578 374 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3388 4134 4380 669 Size: 988 281 1450 746 246 3079 Fragments arranged by size: 3079 1450 988 746 281 246 HindIII A'AGCT_T Cuts at: 748 3153 748 Size: 2405 4385 HpaI GTT'AAC Cuts at: 4380 4380 Size: 6790 KasI G'GCGC_C Cuts at: 2077 2077 Size: 6790 KpnI G_GTAC'C Cuts at: 4115 4115 Size: 6790 MfeI C'AATT_G Cuts at: 4389 4389 Size: 6790 MluI A'CGCG_T Cuts at: 6666 6666 Size: 6790 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2249 2957 3221 3793 5904 6088 1305 Size: 944 708 264 572 2111 184 2007 Fragments arranged by size: 2111 2007 944 708 572 264 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3911 10 Size: 54 1425 343 2079 2889 Fragments arranged by size: 2889 2079 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2309 3905 4047 4748 5107 5266 518 Size: 648 1143 1596 142 701 359 159 2042 Fragments arranged by size: 2042 1596 1143 701 648 359 159 142 MspA1I CmG'CkG Cuts at: 1606 2024 2329 2762 3025 3190 4206 4883 6031 Size: 418 305 433 263 165 1016 677 1148 Cuts at: 6031 6276 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 1016 677 433 418 305 263 245 165 NaeI GCC'GGC Cuts at: 2098 2098 Size: 6790 NarI GG'CG_CC Cuts at: 2078 2078 Size: 6790 NcoI C'CATG_G Cuts at: 513 1065 2004 2590 3144 4042 513 Size: 552 939 586 554 898 3261 Fragments arranged by size: 3261 939 898 586 554 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6790 NgoMIV G'CCGG_C Cuts at: 2096 2096 Size: 6790 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6790 NotI GC'GGCC_GC Cuts at: 2025 4237 2025 Size: 2212 4578 NsiI A_TGCA'T Cuts at: 2190 2190 Size: 6790 NspI r_CATG'y Cuts at: 1132 1229 2192 2526 2563 2999 3377 4148 5693 Size: 97 963 334 37 436 378 771 1545 Cuts at: 5693 1132 Size: 2229 Fragments arranged by size: 2229 1545 963 771 436 378 334 97 37 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6790 PciI A'CATG_T Cuts at: 5689 5689 Size: 6790 PflMI CCAn_nnn'nTGG Cuts at: 1108 3145 1108 Size: 2037 4753 PmeI GTTT'AAAC Cuts at: 4094 4094 Size: 6790 PmlI CAC'GTG Cuts at: 3439 3439 Size: 6790 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4859 4891 6372 6404 4859 Size: 32 1481 32 5245 Fragments arranged by size: 5245 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3237 3864 1613 Size: 1624 627 4539 Fragments arranged by size: 4539 1624 627 PsiI TTA'TAA Cuts at: 2712 4360 2712 Size: 1648 5142 PspOMI G'GGCC_C Cuts at: 3844 3844 Size: 6790 PspXI vC'TCGA_Gb Cuts at: 3821 3821 Size: 6790 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 4046 4078 4046 Size: 32 6758 PstI C_TGCA'G Cuts at: 830 2983 4142 830 Size: 2153 1159 3478 Fragments arranged by size: 3478 2153 1159 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6790 PvuII CAG'CTG Cuts at: 2762 3025 3190 2762 Size: 263 165 6362 Fragments arranged by size: 6362 263 165 SacI G_AGCT'C Cuts at: 3528 3605 3528 Size: 77 6713 SacII CC_GC'GG Cuts at: 2025 2025 Size: 6790 SalI G'TCGA_C Cuts at: 1936 4132 1936 Size: 2196 4594 SapI GCTCTTCn'nnn_ Cuts at: 3822 4008 3822 Size: 186 6604 SbfI CC_TGCA'GG Cuts at: 4142 4142 Size: 6790 ScaI AGT'ACT Cuts at: 2273 5038 6750 2273 Size: 2765 1712 2313 Fragments arranged by size: 2765 2313 1712 SfcI C'TryA_G Cuts at: 826 1045 2297 2979 4138 5273 5954 6145 826 Size: 219 1252 682 1159 1135 681 191 1471 Fragments arranged by size: 1471 1252 1159 1135 682 681 219 191 SfoI GGC'GCC Cuts at: 2079 2079 Size: 6790 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6790 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6790 SmaI CCC'GGG Cuts at: 1872 4117 1872 Size: 2245 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3821 4894 5795 6057 820 Size: 197 926 1878 1073 901 262 1553 Fragments arranged by size: 1878 1553 1073 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4581 493 Size: 4088 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6790 SphI G_CATG'C Cuts at: 1132 2563 2999 4148 1132 Size: 1431 436 1149 3774 Fragments arranged by size: 3774 1431 1149 436 SspI AAT'ATT Cuts at: 5 52 4714 5 Size: 47 4662 2081 Fragments arranged by size: 4662 2081 47 StuI AGG'CCT Cuts at: 1517 3311 3819 1517 Size: 1794 508 4488 Fragments arranged by size: 4488 1794 508 StyI C'CwwG_G Cuts at: 513 1065 2004 2085 2590 3144 4042 4229 513 Size: 552 939 81 505 554 898 187 3074 Fragments arranged by size: 3074 939 898 554 552 505 187 81 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2399 2829 4817 4834 4987 5172 5617 Size: 1255 819 430 1988 17 153 185 445 Cuts at: 5617 325 Size: 1498 Fragments arranged by size: 1988 1498 1255 819 445 430 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2271 5036 6748 Size: 274 80 33 51 1443 294 2765 1712 Cuts at: 6748 96 Size: 138 Fragments arranged by size: 2765 1712 1443 294 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3666 3698 6750 6782 3666 Size: 32 3052 32 3674 Fragments arranged by size: 3674 3052 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6790 XbaI T'CTAG_A Cuts at: 4126 4126 Size: 6790 XhoI C'TCGA_G Cuts at: 1943 3821 1943 Size: 1878 4912 XmaI C'CCGG_G Cuts at: 1870 4115 1870 Size: 2245 4545 XmnI GAAnn'nnTTC Cuts at: 2262 2265 4919 2262 Size: 3 2654 4133 Fragments arranged by size: 4133 2654 3 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3592 4598 276 Size: 53 83 186 936 2058 1006 2468 Fragments arranged by size: 2468 2058 1006 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FseI FspAI NruI PacI PfoI PpuMI RsrII SanDI SexAI SfiI SrfI SwaI XcmI