FEM1B ORF clone FHC01595

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC01595

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Clone info.

Species

human

Clone Name

pFN21ASDA0396 Sequence (with vector) Restriction Map

Product ID

FHC01595

Flexi Clone PID

FXC01595

ORF Sequence

DNA sequence (1881 bp)
Protein sequence (627 aa)

Linker Type

Flexi_RBS type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB383873]

Gene

KIAA0396

Symbol

FEM1B

Alias : F1A-ALPHA, F1AA, FEM1-beta

Description

fem-1 homolog b (C. elegans)

Original Clone ID

hg00180s1

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1881 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 627 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS10228.1 1.1e-205 100.0 FEM1B CCDS4118.1 1.9e-65 40.7 FEM1C CCDS12135.1 8.1e-29 35.8 FEM1A CCDS6121.1 3e-12 36.4 ANK1 CCDS6119.1 3e-12 36.4 ANK1 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan NULL 187 202 PR01415 NULL NULL 514 528 PR01415 NULL HMMPfam IPR002110 48 68 PF00023 Ankyrin repeat IPR002110 87 118 PF00023 Ankyrin repeat IPR002110 123 152 PF00023 Ankyrin repeat IPR002110 154 183 PF00023 Ankyrin repeat IPR002110 187 209 PF00023 Ankyrin repeat IPR002110 484 530 PF00023 Ankyrin repeat HMMSmart IPR002110 45 74 SM00248 Ankyrin repeat IPR002110 87 116 SM00248 Ankyrin repeat IPR002110 120 149 SM00248 Ankyrin repeat IPR002110 153 182 SM00248 Ankyrin repeat IPR002110 186 215 SM00248 Ankyrin repeat IPR002110 218 246 SM00248 Ankyrin repeat IPR002110 483 527 SM00248 Ankyrin repeat IPR002110 531 570 SM00248 Ankyrin repeat ProfileScan IPR002110 45 68 PS50088 Ankyrin repeat IPR020683 45 241 PS50297 Ankyrin repeat-containing domain IPR002110 87 119 PS50088 Ankyrin repeat IPR002110 120 152 PS50088 Ankyrin repeat IPR002110 153 185 PS50088 Ankyrin repeat IPR002110 186 218 PS50088 Ankyrin repeat IPR002110 483 530 PS50088 Ankyrin repeat IPR020683 483 577 PS50297 Ankyrin repeat-containing domain
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_056137 627 613539 100.0 100.0 XP_005272092 617 608767 40.7 98.9 NP_064562 617 608767 40.7 98.9 NP_061178 669 613538 35.8 96.5 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21ASDA0396 Download

>KIAA0396  1881 bp
ATGGAGGGCCTGGCTGGCTATGTATACAAGGCGGCCAGCGAGGGCAAGGTGCTGACTCTG
GCCGCCTTGCTTCTCAACCGGTCTGAAAGCGACATCCGCTATCTGCTTGGCTATGTCAGC
CAGCAGGGAGGGCAGCGCTCCACGCCCCTCATCATCGCAGCCCGCAATGGACACGCAAAG
GTGGTACGCTTGCTCTTAGAACATTACCGGGTGCAGACTCAGCAGACTGGCACCGTCCGC
TTCGACGGGTATGTCATTGATGGTGCCACTGCTCTTTGGTGTGCAGCTGGAGCAGGACAT
TTTGAAGTTGTTAAACTTCTAGTCAGCCATGGAGCCAACGTGAACCATACCACAGTAACT
AATTCAACCCCCCTGCGGGCAGCATGCTTTGATGGCAGACTGGACATTGTGAAATACTTG
GTTGAAAATAATGCCAACATCAGCATTGCCAACAAATATGACAACACCTGCCTAATGATT
GCGGCATATAAGGGACACACTGATGTGGTCAGATACCTTTTAGAACAACGTGCTGATCCC
AATGCCAAAGCACATTGTGGAGCCACAGCATTGCACTTTGCAGCTGAAGCTGGGCACATA
GATATTGTGAAAGAGCTGATAAAATGGCGTGCTGCTATAGTAGTGAATGGCCATGGGATG
ACGCCATTGAAAGTAGCTGCCGAAAGCTGTAAAGCTGATGTCGTAGAACTGTTACTCTCT
CATGCTGATTGCGACCGAAGAAGTCGGATTGAAGCTTTGGAACTCTTGGGTGCCTCCTTT
GCAAATGACCGTGAGAACTATGACATCATAAAGACATACCACTATCTATATTTAGCCATG
TTAGAGAGGTTCCAAGATGGTGATAACATTCTCGAAAAAGAGGTTCTTCCACCAATCCAT
GCTTATGGGAATAGAACTGAATGTAGAAATCCTCAGGAACTGGAGTCCATTCGGCAAGAC
AGAGATGCTCTTCATATGGAAGGCCTTATAGTTCGGGAACGGATTTTAGGTGCTGACAAT
ATTGATGTTTCTCATCCCATCATTTACAGAGGAGCTGTTTATGCGGATAATATGGAATTT
GAGCAGTGTATCAAGTTGTGGCTTCATGCCCTGCACCTCAGACAAAAAGGTAACAGGAAC
ACCCACAAGGATCTTCTTCGATTTGCTCAAGTTTTCTCACAAATGATACATTTGAATGAA
ACTGTGAAGGCCCCAGACATAGAATGTGTTTTGAGATGCAGTGTTTTGGAAATAGAACAA
AGTATGAACAGAGTGAAAAATATTTCAGATGCTGATGTCCACAATGCTATGGACAATTAT
GAATGTAATCTCTATACCTTTCTGTATTTAGTGTGCATCTCTACCAAAACACAGTGCAGC
GAAGAAGATCAGTGCAAAATTAACAAGCAGATCTACAACCTGATTCACCTTGATCCCAGA
ACTCGTGAAGGTTTCACCTTGCTGCATCTGGCTGTCAACTCCAATACTCCAGTTGATGAT
TTCCACACCAATGACGTCTGCAGCTTTCCAAATGCACTTGTCACAAAGCTCCTGCTGGAC
TGTGGTGCTGAGGTGAATGCCGTGGACAATGAGGGAAACAGTGCCCTTCATATTATCGTT
CAGTACAACAGGCCCATCAGTGATTTTTTGACCTTGCACTCCATCATCATTAGCCTAGTT
GAAGCCGGAGCTCACACTGACATGACGAATAAACAGAATAAGACTCCGCTAGACAAAAGT
ACAACTGGGGTATCTGAAATACTGCTTAAAACTCAAATGAAGATGAGTCTCAAGTGCCTG
GCTGCCCGAGCAGTTCGGGCTAATGACATTAACTACCAAGACCAGATCCCCAGAACTCTT
GAAGAGTTTGTTGGATTTCAT

ORF Protein

Cloned ORF protein sequence for pFN21ASDA0396 Download

>KIAA0396  627 aa
MEGLAGYVYKAASEGKVLTLAALLLNRSESDIRYLLGYVSQQGGQRSTPLIIAARNGHAK
VVRLLLEHYRVQTQQTGTVRFDGYVIDGATALWCAAGAGHFEVVKLLVSHGANVNHTTVT
NSTPLRAACFDGRLDIVKYLVENNANISIANKYDNTCLMIAAYKGHTDVVRYLLEQRADP
NAKAHCGATALHFAAEAGHIDIVKELIKWRAAIVVNGHGMTPLKVAAESCKADVVELLLS
HADCDRRSRIEALELLGASFANDRENYDIIKTYHYLYLAMLERFQDGDNILEKEVLPPIH
AYGNRTECRNPQELESIRQDRDALHMEGLIVRERILGADNIDVSHPIIYRGAVYADNMEF
EQCIKLWLHALHLRQKGNRNTHKDLLRFAQVFSQMIHLNETVKAPDIECVLRCSVLEIEQ
SMNRVKNISDADVHNAMDNYECNLYTFLYLVCISTKTQCSEEDQCKINKQIYNLIHLDPR
TREGFTLLHLAVNSNTPVDDFHTNDVCSFPNALVTKLLLDCGAEVNAVDNEGNSALHIIV
QYNRPISDFLTLHSIIISLVEAGAHTDMTNKQNKTPLDKSTTGVSEILLKTQMKMSLKCL
AARAVRANDINYQDQIPRTLEEFVGFH

ORF+Vector

Nucleotide Sequence (with vector) for pFN21ASDA0396 Download

>pFN21ASDA0396  6610 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCTTTCGAAGGAGATAGAACCATGGAGGGCCTGGCTGG
CTATGTATACAAGGCGGCCAGCGAGGGCAAGGTGCTGACTCTGGCCGCCTTGCTTCTCAA
CCGGTCTGAAAGCGACATCCGCTATCTGCTTGGCTATGTCAGCCAGCAGGGAGGGCAGCG
CTCCACGCCCCTCATCATCGCAGCCCGCAATGGACACGCAAAGGTGGTACGCTTGCTCTT
AGAACATTACCGGGTGCAGACTCAGCAGACTGGCACCGTCCGCTTCGACGGGTATGTCAT
TGATGGTGCCACTGCTCTTTGGTGTGCAGCTGGAGCAGGACATTTTGAAGTTGTTAAACT
TCTAGTCAGCCATGGAGCCAACGTGAACCATACCACAGTAACTAATTCAACCCCCCTGCG
GGCAGCATGCTTTGATGGCAGACTGGACATTGTGAAATACTTGGTTGAAAATAATGCCAA
CATCAGCATTGCCAACAAATATGACAACACCTGCCTAATGATTGCGGCATATAAGGGACA
CACTGATGTGGTCAGATACCTTTTAGAACAACGTGCTGATCCCAATGCCAAAGCACATTG
TGGAGCCACAGCATTGCACTTTGCAGCTGAAGCTGGGCACATAGATATTGTGAAAGAGCT
GATAAAATGGCGTGCTGCTATAGTAGTGAATGGCCATGGGATGACGCCATTGAAAGTAGC
TGCCGAAAGCTGTAAAGCTGATGTCGTAGAACTGTTACTCTCTCATGCTGATTGCGACCG
AAGAAGTCGGATTGAAGCTTTGGAACTCTTGGGTGCCTCCTTTGCAAATGACCGTGAGAA
CTATGACATCATAAAGACATACCACTATCTATATTTAGCCATGTTAGAGAGGTTCCAAGA
TGGTGATAACATTCTCGAAAAAGAGGTTCTTCCACCAATCCATGCTTATGGGAATAGAAC
TGAATGTAGAAATCCTCAGGAACTGGAGTCCATTCGGCAAGACAGAGATGCTCTTCATAT
GGAAGGCCTTATAGTTCGGGAACGGATTTTAGGTGCTGACAATATTGATGTTTCTCATCC
CATCATTTACAGAGGAGCTGTTTATGCGGATAATATGGAATTTGAGCAGTGTATCAAGTT
GTGGCTTCATGCCCTGCACCTCAGACAAAAAGGTAACAGGAACACCCACAAGGATCTTCT
TCGATTTGCTCAAGTTTTCTCACAAATGATACATTTGAATGAAACTGTGAAGGCCCCAGA
CATAGAATGTGTTTTGAGATGCAGTGTTTTGGAAATAGAACAAAGTATGAACAGAGTGAA
AAATATTTCAGATGCTGATGTCCACAATGCTATGGACAATTATGAATGTAATCTCTATAC
CTTTCTGTATTTAGTGTGCATCTCTACCAAAACACAGTGCAGCGAAGAAGATCAGTGCAA
AATTAACAAGCAGATCTACAACCTGATTCACCTTGATCCCAGAACTCGTGAAGGTTTCAC
CTTGCTGCATCTGGCTGTCAACTCCAATACTCCAGTTGATGATTTCCACACCAATGACGT
CTGCAGCTTTCCAAATGCACTTGTCACAAAGCTCCTGCTGGACTGTGGTGCTGAGGTGAA
TGCCGTGGACAATGAGGGAAACAGTGCCCTTCATATTATCGTTCAGTACAACAGGCCCAT
CAGTGATTTTTTGACCTTGCACTCCATCATCATTAGCCTAGTTGAAGCCGGAGCTCACAC
TGACATGACGAATAAACAGAATAAGACTCCGCTAGACAAAAGTACAACTGGGGTATCTGA
AATACTGCTTAAAACTCAAATGAAGATGAGTCTCAAGTGCCTGGCTGCCCGAGCAGTTCG
GGCTAATGACATTAACTACCAAGACCAGATCCCCAGAACTCTTGAAGAGTTTGTTGGATT
TCATTACGTAGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTG
CAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTG
CCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATA
AGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATT
TGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTT
AACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTT
TTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCG
CTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGT
TTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGA
AATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTC
ATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATT
CAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCT
CACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGT
TACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGT
TTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGAC
GCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTAC
TCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCT
GCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCG
AAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGG
GAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCA
ATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAA
CAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTT
CCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATC
ATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGG
AGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATT
AAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAA
AGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAA
AAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGC
TCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGA
CAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTC
CGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTT
CTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCT
GTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTG
AGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTA
GCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCT
ACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAA
GAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTT
GCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTA
CGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTAT
CAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGAT
GGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGG
TAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGC
GATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGC
GCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCT
GCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGG
ATCGAGATCT