MARCH4 ORF clone FHC00826

KOP
KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC00826

How to Order Order Information

Clone info.

Species

human

Clone Name

pFN21ASDA1399 Sequence (with vector) Restriction Map

Product ID

FHC00826

Flexi Clone PID

FXC00826

ORF Sequence

DNA sequence (1230 bp)
Protein sequence (410 aa)

Linker Type

Flexi_RBS type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB384199]

Gene

KIAA1399

Symbol

MARCH4

Description

membrane-associated ring finger (C3HC4) 4, E3 ubiquitin protein ligase

Original Clone ID

hk07377

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1230 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 410 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS33376.1 4.1e-98 100.0 MARCH4 CCDS31847.1 6.3e-36 58.3 MARCH9 CCDS47192.1 2.4e-28 47.2 MARCH11 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR018957 163 208 PF00097 Zinc finger HMMSmart IPR011016 162 209 SM00744 Zinc finger ProfileScan IPR011016 155 215 PS51292 Zinc finger
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_065865 410 608208 100.0 100.0 NP_612405 346 613336 58.3 91.2 NP_001096032 402 613338 47.2 86.3 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21ASDA1399 Download

>KIAA1399  1230 bp
ATGCTGATGCCCCTGTGTGGGCTGCTCTGGTGGTGGTGGTGCTGCTGCTCCGGCTGGTAC
TGCTATGGATTGTGTGCCCCAGCCCCCCAGATGTTGCGCCACCAGGGTCTCCTCAAGTGC
CGCTGCCGCATGCTCTTCAATGACCTGAAGGTTTTCTTACTGCGGCGCCCTCCTCAAGCG
CCCCTGCCCATGCACGGCGACCCCCAGCCCCCCGGTTTGGCGGCCAACAACACCCTTCCG
GCTCTGGGCGCCGGGGGGTGGGCAGGCTGGAGGGGCCCCCGAGAAGTGGTGGGCAGGGAG
CCCCCTCCTGTGCCACCTCCACCCCCCTTGCCACCTTCTTCTGTGGAAGATGACTGGGGT
GGCCCAGCCACAGAGCCACCTGCCTCGCTGCTCAGCAGTGCCTCCTCAGATGACTTCTGT
AAGGAGAAGACCGAGGATCGCTACTCACTGGGCAGCAGCTTGGACAGTGGTATGAGGACC
CCACTCTGCCGCATCTGCTTCCAGGGGCCAGAACAGGGGGAGCTGCTGAGCCCATGCCGC
TGTGATGGCTCGGTCAAGTGCACACACCAGCCTTGCCTCATCAAGTGGATCAGCGAGCGG
GGCTGCTGGAGCTGCGAGCTGTGCTACTACAAGTACCACGTCATCGCCATAAGCACAAAA
AATCCTCTGCAGTGGCAGGCCATCTCTCTGACGGTCATTGAGAAGGTTCAGGTTGCAGCC
GCCATCCTGGGCTCCCTCTTCCTCATCGCCAGTATTTCTTGGCTCATCTGGTCAACTTTC
AGCCCCTCGGCAAGATGGCAGCGCCAAGACCTTCTCTTCCAGATCTGCTACGGGATGTAT
GGCTTCATGGACGTGGTGTGCATAGGTCTCATCATCCATGAAGGACCCTCGGTGTACCGC
ATCTTTAAACGGTGGCAGGCTGTCAACCAGCAGTGGAAAGTGCTGAACTATGACAAGACA
AAAGACCTGGAGGATCAAAAGGCAGGAGGCAGGACCAACCCCCGGACCTCCTCATCCACC
CAGGCCAATATCCCCTCCTCGGAAGAGGAGACCGCAGGCACCCCTGCCCCTGAGCAGGGC
CCTGCCCAGGCTGCCGGCCACCCCTCAGGCCCTCTGTCCCATCACCACTGTGCTTATACC
ATCCTGCACATCCTGAGTCACTTGAGACCTCATGAACAGCGAAGTCCCCCAGGCAGCAGC
CGAGAGCTGGTCATGAGAGTCACGACAGTG

ORF Protein

Cloned ORF protein sequence for pFN21ASDA1399 Download

>KIAA1399  410 aa
MLMPLCGLLWWWWCCCSGWYCYGLCAPAPQMLRHQGLLKCRCRMLFNDLKVFLLRRPPQA
PLPMHGDPQPPGLAANNTLPALGAGGWAGWRGPREVVGREPPPVPPPPPLPPSSVEDDWG
GPATEPPASLLSSASSDDFCKEKTEDRYSLGSSLDSGMRTPLCRICFQGPEQGELLSPCR
CDGSVKCTHQPCLIKWISERGCWSCELCYYKYHVIAISTKNPLQWQAISLTVIEKVQVAA
AILGSLFLIASISWLIWSTFSPSARWQRQDLLFQICYGMYGFMDVVCIGLIIHEGPSVYR
IFKRWQAVNQQWKVLNYDKTKDLEDQKAGGRTNPRTSSSTQANIPSSEEETAGTPAPEQG
PAQAAGHPSGPLSHHHCAYTILHILSHLRPHEQRSPPGSSRELVMRVTTV

ORF+Vector

Nucleotide Sequence (with vector) for pFN21ASDA1399 Download

>pFN21ASDA1399  5959 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCTTTCGAAGGAGATAGAACCATGCTGATGCCCCTGTG
TGGGCTGCTCTGGTGGTGGTGGTGCTGCTGCTCCGGCTGGTACTGCTATGGATTGTGTGC
CCCAGCCCCCCAGATGTTGCGCCACCAGGGTCTCCTCAAGTGCCGCTGCCGCATGCTCTT
CAATGACCTGAAGGTTTTCTTACTGCGGCGCCCTCCTCAAGCGCCCCTGCCCATGCACGG
CGACCCCCAGCCCCCCGGTTTGGCGGCCAACAACACCCTTCCGGCTCTGGGCGCCGGGGG
GTGGGCAGGCTGGAGGGGCCCCCGAGAAGTGGTGGGCAGGGAGCCCCCTCCTGTGCCACC
TCCACCCCCCTTGCCACCTTCTTCTGTGGAAGATGACTGGGGTGGCCCAGCCACAGAGCC
ACCTGCCTCGCTGCTCAGCAGTGCCTCCTCAGATGACTTCTGTAAGGAGAAGACCGAGGA
TCGCTACTCACTGGGCAGCAGCTTGGACAGTGGTATGAGGACCCCACTCTGCCGCATCTG
CTTCCAGGGGCCAGAACAGGGGGAGCTGCTGAGCCCATGCCGCTGTGATGGCTCGGTCAA
GTGCACACACCAGCCTTGCCTCATCAAGTGGATCAGCGAGCGGGGCTGCTGGAGCTGCGA
GCTGTGCTACTACAAGTACCACGTCATCGCCATAAGCACAAAAAATCCTCTGCAGTGGCA
GGCCATCTCTCTGACGGTCATTGAGAAGGTTCAGGTTGCAGCCGCCATCCTGGGCTCCCT
CTTCCTCATCGCCAGTATTTCTTGGCTCATCTGGTCAACTTTCAGCCCCTCGGCAAGATG
GCAGCGCCAAGACCTTCTCTTCCAGATCTGCTACGGGATGTATGGCTTCATGGACGTGGT
GTGCATAGGTCTCATCATCCATGAAGGACCCTCGGTGTACCGCATCTTTAAACGGTGGCA
GGCTGTCAACCAGCAGTGGAAAGTGCTGAACTATGACAAGACAAAAGACCTGGAGGATCA
AAAGGCAGGAGGCAGGACCAACCCCCGGACCTCCTCATCCACCCAGGCCAATATCCCCTC
CTCGGAAGAGGAGACCGCAGGCACCCCTGCCCCTGAGCAGGGCCCTGCCCAGGCTGCCGG
CCACCCCTCAGGCCCTCTGTCCCATCACCACTGTGCTTATACCATCCTGCACATCCTGAG
TCACTTGAGACCTCATGAACAGCGAAGTCCCCCAGGCAGCAGCCGAGAGCTGGTCATGAG
AGTCACGACAGTGTACGTAGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGA
GTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGT
TGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCA
GACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAA
TGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAAT
AAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGG
GAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATC
CTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGT
CAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACT
TTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATG
TATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT
ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCT
GTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCA
CGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCC
GAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCC
CGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTG
GTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTA
TGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATC
GGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTT
GATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATG
CCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCT
TCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGC
TCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCT
CGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTAC
ACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCC
TCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAG
CTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACA
TGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTT
TCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGC
GAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCT
CTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCG
TGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCA
AGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACT
ATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTA
ACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTA
ACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCT
TCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTT
TTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGA
TCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCA
TGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGC
ACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATT
AAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTT
ATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGC
CGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCAC
TTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCG
GCGTAGAGGATCGAGATCT