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SpecieshumanProduct IDFHC00207Cloning SiteSgfI-PmeISymbolITSN2
Alias : PRO2015, SH3D1B, SH3P18, SWA, SWAPDescriptionintersectin 2, transcript variant 3
Characterization of the cloned ORF
N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand
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Length: 5010 bp
Restriction map A
Restriction map B
Genomic Structure
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Length: 1670 aa
Result of homology search against CCDS protein database (
FASTA output,
Multiple alignment)
Entry Exp ID% symbol CCDS1711.2 0 99.9 ITSN2 CCDS1710.2 7.6e-146 98.4 ITSN2 CCDS46230.1 3.2e-81 97.3 ITSN2 CCDS82664.1 5.2e-75 60.2 ITSN1 CCDS33545.1 2.6e-74 60.1 ITSN1
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)
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Result of InterProScan
Search method interpro_ID From To Entry Definition FPrintScan IPR000108 1044 1063 PR00499 Neutrophil cytosol factor 2 p67phox IPR001452 1103 1113 PR00452 Src homology-3 domain IPR000108 1105 1125 PR00499 Neutrophil cytosol factor 2 p67phox IPR001452 1117 1132 PR00452 Src homology-3 domain IPR000108 1125 1141 PR00499 Neutrophil cytosol factor 2 p67phox IPR001452 1134 1143 PR00452 Src homology-3 domain IPR000108 1141 1154 PR00499 Neutrophil cytosol factor 2 p67phox IPR001452 1145 1157 PR00452 Src homology-3 domain HMMPfam IPR001452 736 783 PF00018 Src homology-3 domain IPR011511 876 926 PF07653 Variant SH3 IPR001452 960 1004 PF00018 Src homology-3 domain IPR001452 1036 1082 PF00018 Src homology-3 domain IPR001452 1106 1151 PF00018 Src homology-3 domain IPR000219 1187 1367 PF00621 Dbl homology (DH) domain IPR000008 1544 1624 PF00168 C2 calcium-dependent membrane targeting HMMSmart IPR000261 15 109 SM00027 EPS15 homology (EH) IPR002048 58 86 SM00054 Calcium-binding EF-hand IPR000261 237 332 SM00027 EPS15 homology (EH) IPR002048 281 309 SM00054 Calcium-binding EF-hand IPR001452 733 790 SM00326 Src homology-3 domain IPR001452 874 928 SM00326 Src homology-3 domain IPR001452 957 1011 SM00326 Src homology-3 domain IPR001452 1029 1089 SM00326 Src homology-3 domain IPR001452 1103 1158 SM00326 Src homology-3 domain IPR000219 1186 1367 SM00325 Dbl homology (DH) domain IPR001849 1408 1519 SM00233 Pleckstrin homology domain IPR000008 1543 1640 SM00239 C2 calcium-dependent membrane targeting ProfileScan IPR000261 22 110 PS50031 EPS15 homology (EH) IPR018249 54 89 PS50222 EF-HAND 2 IPR000261 244 333 PS50031 EPS15 homology (EH) IPR018249 277 312 PS50222 EF-HAND 2 IPR001452 730 791 PS50002 Src homology-3 domain IPR001452 871 929 PS50002 Src homology-3 domain IPR001452 954 1012 PS50002 Src homology-3 domain IPR001452 1026 1090 PS50002 Src homology-3 domain IPR001452 1100 1159 PS50002 Src homology-3 domain IPR000219 1182 1368 PS50010 Dbl homology (DH) domain IPR001849 1407 1517 PS50003 Pleckstrin homology domain IPR018029 1543 1625 PS50004 C2 membrane targeting protein
Result of homology search against disease genes in OMIM database(FASTA output,
Multiple alignment)
H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_062541 1670 604464 99.9 100.0 NP_006268 1697 604464 98.4 100.0 XP_016883922 1650 602442 58.2 99.7 XP_016883924 1613 602442 57.5 99.7 XP_016883923 1645 602442 58.3 99.7 Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.comCloned ORF Nucleotide sequence for pFN21AA1256 Download>KIAA1256 5010 bp ATGATGGCTCAGTTTCCCACAGCTATGAATGGAGGGCCAAACATGTGGGCTATTACCTCT GAAGAACGTACTAAGCATGACAGGCAGTTTGATAACCTCAAACCTTCAGGAGGTTACATA ACAGGTGATCAAGCACGTAATTTTTTCCTACAATCAGGTCTGCCGGCCCCTGTTTTAGCT GAAATATGGGCTTTATCAGACCTAAACAAGGATGGGAAGATGGATCAGCAAGAGTTCTCC ATAGCTATGAAACTCATCAAACTGAAGCTTCAAGGCCAACAGTTGCCTGTGGTTCTCCCT CCTATTATGAAGCAACCCCCTATGTTTTCTCCATTAATTTCTGCTCGTTTTGGAATGGGA AGCATGCCCAATCTGTCCATTCCTCAGCCATTGCCTCCAGCTGCACCTATAACATCATTG TCTTCTGCGACTTCAGGGACCAACCTTCCTCCCTTAATGATGCCCACTCCCCTAGTGCCT TCTGTTAGCACATCATCATTACCAAATGGAACCGCCAGTCTCATTCAGCCTTTACCCATT CCTTATTCTTCTTCAACATTGCCTCATGGGTCATCTTATAGTCTGATGATGGGAGGATTT GGAGGTGCTAGTATACAGAAAGCGCAGTCTCTGATTGATTTAGGATCTAGTAGCTCAACT TCCTCGACTGCTTCACTCTCAGGGAACTCACCCAAGACTGGGACCTCAGAGTGGGCAGTT CCTCAGCCTACAAGATTAAAATATCGGCAAAAATTTAATACTCTTGACAAAAGTATGAGT GGATATCTCTCAGGTTTTCAAGCTAGAAATGCCCTTCTTCAGTCAAATCTTTCTCAAACT CAGCTGGCTACTATTTGGACTCTGGCTGACGTTGATGGTGATGGACAGCTAAAAGCAGAA GAGTTTATTCTTGCAATGCACCTTACTGACATGGCCAAAGCTGGACAGCCATTACCACTG ACTTTACCTCCTGAGCTTGTTCCTCCATCTTTCAGAGGAGGAAAGCAAATTGATTCCATT AATGGAACTCTGCCTTCATATCAGAAAATGCAAGAAGAGGAGCCTCAGAAGAAATTACCA GTTACTTTTGAGGACAAACGGAAAGCCAACTATGAGCGAGGGAACATGGAGCTGGAAAAG CGACGCCAAGCCTTGATGGAGCAGCAACAAAGGGAGGCAGAACGTAAAGCCCAGAAAGAA AAGGAAGAGTGGGAACGAAAACAGAGAGAATTACAAGAACAAGAATGGAAGAAACAACTT GAATTAGAAAAACGCTTAGAGAAGCAACGGGAATTGGAGAGACAACGAGAGGAAGAAAGG AGAAAAGACATAGAAAGACGAGAGGCAGCAAAACAGGAACTTGAACGACAACGTCGCTTA GAATGGGAGAGAATTCGGCGACAGGAGCTTCTCAATCAAAAGAATAGAGAACAAGAAGAA ATTGTCAGGTTAAACTCTAAAAAGAAGAATCTTCATCTTGAGTTGGAAGCACTGAATGGC AAACATCAGCAGATCTCAGGCAGACTTCAGGATGTCCGACTCAAAAAGCAAACTCAAAAG ACTGAGCTGGAAGTTCTGGATAAGCAGTGTGACTTGGAAATTATGGAAATCAAGCAACTT CAACAGGAACTTCAGGAATATCAGAATAAGCTTATCTATCTGGTACCTGAGAAGCAATTA TTAAATGAAAGAATTAAAAACATGCAGTTCAGTAACACACCTGATTCAGGGGTCAGTTTA CTTCATAAAAAATCATTAGAAAAGGAAGAATTATGCCAAAGACTTAAAGAACAGTTAGAT GCTCTTGAAAAAGAAACTGCATCTAAGCTGTCAGAAATGGATTCTTTTAACAATCAACTA AAGGAACTGAGAGAAACCTACAACACACAGCAGTTAGCCCTTGAACAGCTTTATAAGATC AAACGTGACAAGTTGAAGGAAATTGAAAGGAAAAGATTAGAACTAATGCAGAAAAAGAAA CTAGAAGATGAGGCTGCAAGGAAAGCAAAGCAAGGAAAAGAAAACTTATGGAAAGAAAAT CTTAGAAAGGAGGAAGAAGAAAAACAAAAGCGACTCCAGGAAGAAAAAACACAAGAAAAA ATTCAAGAAGAGGAACGGAAAGCTGAGGAGAAACAACGTAAGGATAAGGATACTTTGAAA GCTGAGGAGAAAAAACGTGAGACAGCTAGTGTTTTGGTGAATTATAGAGCATTATACCCC TTTGAAGCAAGGAACCATGATGAGATGAGTTTTAATTCTGGAGATATAATTCAGGTTGAT GAAAAAACCGTAGGAGAACCTGGTTGGCTTTATGGTAGTTTTCAAGGAAATTTTGGCTGG TTTCCATGCAATTATGTAGAAAAAATGCCATCAAGTGAAAATGAAAAAGCTGTATCTCCA AAGAAGGCCTTACTTCCTCCTACAGTTTCTTTATCTGCTACCTCAACTTCCTCTGAACCA CTTTCTTCAAATCAACCAGCATCAGTGACTGATTATCAAAATGTATCTTTTTCAAACCTA ACTGTAAATACATCATGGCAGAAAAAATCAGCCTTCACTCGAACTGTGTCCCCTGGATCT GTATCACCTATTCATGGACAGGGACAAGTGGTAGAAAACTTAAAAGCACAGGCCCTTTGT TCCTGGACTGCAAAGAAAGATAACCACTTGAACTTCTCAAAACATGACATTATTACTGTC TTGGAGCAGCAAGAAAATTGGTGGTTTGGGGAGGTGCATGGAGGAAGAGGATGGTTTCCC AAATCTTATGTCAAGATCATTCCTGGGAGTGAAGTAAAACGGGAAGAACCAGAAGCTTTG TATGCAGCTGTAAATAAGAAACCTACCTCGGCAGCCTATTCAGTTGGAGAAGAATATATT GCACTTTATCCATATTCAAGTGTGGAACCTGGAGATTTGACTTTCACAGAAGGTGAAGAA ATATTGGTGACCCAGAAAGATGGAGAGTGGTGGACAGGAAGTATTGGAGATAGAAGTGGA ATTTTTCCATCAAACTATGTCAAACCAAAGGATCAAGAGAGTTTTGGGAGTGCTAGCAAG TCTGGAGCATCAAATAAAAAACCTGAGATTGCTCAGGTAACTTCAGCATATGTTGCTTCT GGTTCTGAACAACTTAGCCTTGCACCAGGACAGTTAATATTAATTCTAAAGAAAAATACA AGTGGGTGGTGGCAAGGAGAGTTACAGGCCAGAGGAAAAAAGCGACAGAAAGGATGGTTT CCTGCCAGTCATGTTAAACTTTTGGGTCCAAGTAGTGAAAGAGCCACACCTGCCTTTCAT CCTGTATGTCAGGTGATTGCTATGTATGACTATGCAGCAAATAATGAAGATGAGCTCAGT TTCTCCAAGGGACAACTCATTAATGTTATGAACAAAGATGATCCTGATTGGTGGCAAGGA GAGATCAACGGGGTGACTGGTCTCTTTCCTTCAAACTACGTTAAGATGACGACAGACTCA GATCCAAGTCAACAGTGGTGTGCTGATCTGCAAACCCTGGACACAATGCAGCCAATTGAG AGGAAAAGACAGGGCTATATTCATGAGCTGATTCAGACCGAAGAGCGGTACATGGCTGAC CTTCAGCTCGTCGTCGAGGTTTTTCAGAAACGCATGGCAGAGTCAGGCTTTCTCACTGAA GGGGAGATGGCCCTGATTTTTGTTAACTGGAAGGAGCTCATCATGTCCAACACAAAGCTG CTGAAGGCTTTGCGGGTGCGGAAGAAGACCGGGGGCGAGAAGATGCCGGTGCAGATGATT GGGGACATCCTGGCCGCTGAGCTGTCCCACATGCAGGCTTACATCAGGTTCTGCAGCTGC CAGCTTAATGGAGCAGCTCTGTTACAGCAGAAGACAGATGAAGACACAGATTTCAAAGAA TTTTTAAAGAAGCTGGCATCTGACCCGCGGTGTAAAGGAATGCCCCTCTCCAGCTTCCTG CTGAAACCCATGCAGAGGATCACCCGCTACCCACTGCTCATCAGAAGTATTCTGGAGAAC ACCCCGGAGAGCCATGCAGACCATTCCTCCCTAAAGCTGGCCCTCGAGCGGGCAGAGGAG CTGTGCTCTCAAGTGAATGAGGGAGTTCGGGAGAAGGAAAACTCGGACCGACTGGAGTGG ATCCAGGCGCACGTGCAGTGTGAAGGCCTCGCGGAGCAACTTATTTTCAACTCTCTCACC AACTGCCTGGGGCCCCGGAAGCTCTTACACAGTGGGAAATTATACAAGACCAAGAGCAAC AAGGAACTGCACGGATTCCTCTTCAATGACTTCCTGCTTCTTACCTACATGGTCAAGCAG TTTGCTGTTTCCTCTGGCTCTGAGAAACTTTTCAGCTCGAAGTCCAATGCTCAATTCAAA ATGTATAAAACGCCCATTTTCCTGAATGAAGTCTTGGTGAAACTGCCCACAGACCCTTCC AGCGATGAGCCTGTCTTCCACATTTCCCACATTGATCGGGTCTACACCCTCCGAACAGAC AACATTAATGAGAGGACCGCCTGGGTGCAGAAGATCAAGGCGGCGTCTGAGCAGTACATC GACACCGAGAAGAAGCAGCGTGAGAAAGCTTACCAAGCCCGCTCCCAAAAGACTTCAGGC ATTGGGCGCCTGATGGTGCATGTCATTGAAGCTACAGAATTAAAAGCCTGCAAACCAAAT GGAAAGAGCAACCCATACTGTGAAATCAGCATGGGCTCCCAGAGCTACACCACCAGGACC ATCCAGGACACACTCAATCCCAAGTGGAATTTTAACTGCCAGTTCTTTATTAAGGATCTC TACCAAGACGTGCTGTGTCTCACCCTGTTTGACAGAGACCAGTTTTCACCAGATGATTTC CTGGGTCGTACTGAAATTCCAGTGGCAAAAATTCGAACAGAACAGGAAAGCAAAGGCCCT ATGACCCGCCGACTGCTGCTGCATGAGGTCCCCACCGGGGAGGTCTGGGTCCGTTTTGAC CTGCAGCTTTTTGAGCAAAAAACTCTCCTG
Cloned ORF protein sequence for pFN21AA1256 Download>KIAA1256 1670 aa MMAQFPTAMNGGPNMWAITSEERTKHDRQFDNLKPSGGYITGDQARNFFLQSGLPAPVLA EIWALSDLNKDGKMDQQEFSIAMKLIKLKLQGQQLPVVLPPIMKQPPMFSPLISARFGMG SMPNLSIPQPLPPAAPITSLSSATSGTNLPPLMMPTPLVPSVSTSSLPNGTASLIQPLPI PYSSSTLPHGSSYSLMMGGFGGASIQKAQSLIDLGSSSSTSSTASLSGNSPKTGTSEWAV PQPTRLKYRQKFNTLDKSMSGYLSGFQARNALLQSNLSQTQLATIWTLADVDGDGQLKAE EFILAMHLTDMAKAGQPLPLTLPPELVPPSFRGGKQIDSINGTLPSYQKMQEEEPQKKLP VTFEDKRKANYERGNMELEKRRQALMEQQQREAERKAQKEKEEWERKQRELQEQEWKKQL ELEKRLEKQRELERQREEERRKDIERREAAKQELERQRRLEWERIRRQELLNQKNREQEE IVRLNSKKKNLHLELEALNGKHQQISGRLQDVRLKKQTQKTELEVLDKQCDLEIMEIKQL QQELQEYQNKLIYLVPEKQLLNERIKNMQFSNTPDSGVSLLHKKSLEKEELCQRLKEQLD ALEKETASKLSEMDSFNNQLKELRETYNTQQLALEQLYKIKRDKLKEIERKRLELMQKKK LEDEAARKAKQGKENLWKENLRKEEEEKQKRLQEEKTQEKIQEEERKAEEKQRKDKDTLK AEEKKRETASVLVNYRALYPFEARNHDEMSFNSGDIIQVDEKTVGEPGWLYGSFQGNFGW FPCNYVEKMPSSENEKAVSPKKALLPPTVSLSATSTSSEPLSSNQPASVTDYQNVSFSNL TVNTSWQKKSAFTRTVSPGSVSPIHGQGQVVENLKAQALCSWTAKKDNHLNFSKHDIITV LEQQENWWFGEVHGGRGWFPKSYVKIIPGSEVKREEPEALYAAVNKKPTSAAYSVGEEYI ALYPYSSVEPGDLTFTEGEEILVTQKDGEWWTGSIGDRSGIFPSNYVKPKDQESFGSASK SGASNKKPEIAQVTSAYVASGSEQLSLAPGQLILILKKNTSGWWQGELQARGKKRQKGWF PASHVKLLGPSSERATPAFHPVCQVIAMYDYAANNEDELSFSKGQLINVMNKDDPDWWQG EINGVTGLFPSNYVKMTTDSDPSQQWCADLQTLDTMQPIERKRQGYIHELIQTEERYMAD LQLVVEVFQKRMAESGFLTEGEMALIFVNWKELIMSNTKLLKALRVRKKTGGEKMPVQMI GDILAAELSHMQAYIRFCSCQLNGAALLQQKTDEDTDFKEFLKKLASDPRCKGMPLSSFL LKPMQRITRYPLLIRSILENTPESHADHSSLKLALERAEELCSQVNEGVREKENSDRLEW IQAHVQCEGLAEQLIFNSLTNCLGPRKLLHSGKLYKTKSNKELHGFLFNDFLLLTYMVKQ FAVSSGSEKLFSSKSNAQFKMYKTPIFLNEVLVKLPTDPSSDEPVFHISHIDRVYTLRTD NINERTAWVQKIKAASEQYIDTEKKQREKAYQARSQKTSGIGRLMVHVIEATELKACKPN GKSNPYCEISMGSQSYTTRTIQDTLNPKWNFNCQFFIKDLYQDVLCLTLFDRDQFSPDDF LGRTEIPVAKIRTEQESKGPMTRRLLLHEVPTGEVWVRFDLQLFEQKTLL
Nucleotide Sequence (with vector) for pFN21AA1256 Download>pFN21AA1256 9715 bp TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA CTTTCAGAGCGATAACGCGATCGCCATGATGGCTCAGTTTCCCACAGCTATGAATGGAGG GCCAAACATGTGGGCTATTACCTCTGAAGAACGTACTAAGCATGACAGGCAGTTTGATAA CCTCAAACCTTCAGGAGGTTACATAACAGGTGATCAAGCACGTAATTTTTTCCTACAATC AGGTCTGCCGGCCCCTGTTTTAGCTGAAATATGGGCTTTATCAGACCTAAACAAGGATGG GAAGATGGATCAGCAAGAGTTCTCCATAGCTATGAAACTCATCAAACTGAAGCTTCAAGG CCAACAGTTGCCTGTGGTTCTCCCTCCTATTATGAAGCAACCCCCTATGTTTTCTCCATT AATTTCTGCTCGTTTTGGAATGGGAAGCATGCCCAATCTGTCCATTCCTCAGCCATTGCC TCCAGCTGCACCTATAACATCATTGTCTTCTGCGACTTCAGGGACCAACCTTCCTCCCTT AATGATGCCCACTCCCCTAGTGCCTTCTGTTAGCACATCATCATTACCAAATGGAACCGC CAGTCTCATTCAGCCTTTACCCATTCCTTATTCTTCTTCAACATTGCCTCATGGGTCATC TTATAGTCTGATGATGGGAGGATTTGGAGGTGCTAGTATACAGAAAGCGCAGTCTCTGAT TGATTTAGGATCTAGTAGCTCAACTTCCTCGACTGCTTCACTCTCAGGGAACTCACCCAA GACTGGGACCTCAGAGTGGGCAGTTCCTCAGCCTACAAGATTAAAATATCGGCAAAAATT TAATACTCTTGACAAAAGTATGAGTGGATATCTCTCAGGTTTTCAAGCTAGAAATGCCCT TCTTCAGTCAAATCTTTCTCAAACTCAGCTGGCTACTATTTGGACTCTGGCTGACGTTGA TGGTGATGGACAGCTAAAAGCAGAAGAGTTTATTCTTGCAATGCACCTTACTGACATGGC CAAAGCTGGACAGCCATTACCACTGACTTTACCTCCTGAGCTTGTTCCTCCATCTTTCAG AGGAGGAAAGCAAATTGATTCCATTAATGGAACTCTGCCTTCATATCAGAAAATGCAAGA AGAGGAGCCTCAGAAGAAATTACCAGTTACTTTTGAGGACAAACGGAAAGCCAACTATGA GCGAGGGAACATGGAGCTGGAAAAGCGACGCCAAGCCTTGATGGAGCAGCAACAAAGGGA GGCAGAACGTAAAGCCCAGAAAGAAAAGGAAGAGTGGGAACGAAAACAGAGAGAATTACA AGAACAAGAATGGAAGAAACAACTTGAATTAGAAAAACGCTTAGAGAAGCAACGGGAATT GGAGAGACAACGAGAGGAAGAAAGGAGAAAAGACATAGAAAGACGAGAGGCAGCAAAACA GGAACTTGAACGACAACGTCGCTTAGAATGGGAGAGAATTCGGCGACAGGAGCTTCTCAA TCAAAAGAATAGAGAACAAGAAGAAATTGTCAGGTTAAACTCTAAAAAGAAGAATCTTCA TCTTGAGTTGGAAGCACTGAATGGCAAACATCAGCAGATCTCAGGCAGACTTCAGGATGT CCGACTCAAAAAGCAAACTCAAAAGACTGAGCTGGAAGTTCTGGATAAGCAGTGTGACTT GGAAATTATGGAAATCAAGCAACTTCAACAGGAACTTCAGGAATATCAGAATAAGCTTAT CTATCTGGTACCTGAGAAGCAATTATTAAATGAAAGAATTAAAAACATGCAGTTCAGTAA CACACCTGATTCAGGGGTCAGTTTACTTCATAAAAAATCATTAGAAAAGGAAGAATTATG CCAAAGACTTAAAGAACAGTTAGATGCTCTTGAAAAAGAAACTGCATCTAAGCTGTCAGA AATGGATTCTTTTAACAATCAACTAAAGGAACTGAGAGAAACCTACAACACACAGCAGTT AGCCCTTGAACAGCTTTATAAGATCAAACGTGACAAGTTGAAGGAAATTGAAAGGAAAAG ATTAGAACTAATGCAGAAAAAGAAACTAGAAGATGAGGCTGCAAGGAAAGCAAAGCAAGG AAAAGAAAACTTATGGAAAGAAAATCTTAGAAAGGAGGAAGAAGAAAAACAAAAGCGACT CCAGGAAGAAAAAACACAAGAAAAAATTCAAGAAGAGGAACGGAAAGCTGAGGAGAAACA ACGTAAGGATAAGGATACTTTGAAAGCTGAGGAGAAAAAACGTGAGACAGCTAGTGTTTT GGTGAATTATAGAGCATTATACCCCTTTGAAGCAAGGAACCATGATGAGATGAGTTTTAA TTCTGGAGATATAATTCAGGTTGATGAAAAAACCGTAGGAGAACCTGGTTGGCTTTATGG TAGTTTTCAAGGAAATTTTGGCTGGTTTCCATGCAATTATGTAGAAAAAATGCCATCAAG TGAAAATGAAAAAGCTGTATCTCCAAAGAAGGCCTTACTTCCTCCTACAGTTTCTTTATC TGCTACCTCAACTTCCTCTGAACCACTTTCTTCAAATCAACCAGCATCAGTGACTGATTA TCAAAATGTATCTTTTTCAAACCTAACTGTAAATACATCATGGCAGAAAAAATCAGCCTT CACTCGAACTGTGTCCCCTGGATCTGTATCACCTATTCATGGACAGGGACAAGTGGTAGA AAACTTAAAAGCACAGGCCCTTTGTTCCTGGACTGCAAAGAAAGATAACCACTTGAACTT CTCAAAACATGACATTATTACTGTCTTGGAGCAGCAAGAAAATTGGTGGTTTGGGGAGGT GCATGGAGGAAGAGGATGGTTTCCCAAATCTTATGTCAAGATCATTCCTGGGAGTGAAGT AAAACGGGAAGAACCAGAAGCTTTGTATGCAGCTGTAAATAAGAAACCTACCTCGGCAGC CTATTCAGTTGGAGAAGAATATATTGCACTTTATCCATATTCAAGTGTGGAACCTGGAGA TTTGACTTTCACAGAAGGTGAAGAAATATTGGTGACCCAGAAAGATGGAGAGTGGTGGAC AGGAAGTATTGGAGATAGAAGTGGAATTTTTCCATCAAACTATGTCAAACCAAAGGATCA AGAGAGTTTTGGGAGTGCTAGCAAGTCTGGAGCATCAAATAAAAAACCTGAGATTGCTCA GGTAACTTCAGCATATGTTGCTTCTGGTTCTGAACAACTTAGCCTTGCACCAGGACAGTT AATATTAATTCTAAAGAAAAATACAAGTGGGTGGTGGCAAGGAGAGTTACAGGCCAGAGG AAAAAAGCGACAGAAAGGATGGTTTCCTGCCAGTCATGTTAAACTTTTGGGTCCAAGTAG TGAAAGAGCCACACCTGCCTTTCATCCTGTATGTCAGGTGATTGCTATGTATGACTATGC AGCAAATAATGAAGATGAGCTCAGTTTCTCCAAGGGACAACTCATTAATGTTATGAACAA AGATGATCCTGATTGGTGGCAAGGAGAGATCAACGGGGTGACTGGTCTCTTTCCTTCAAA CTACGTTAAGATGACGACAGACTCAGATCCAAGTCAACAGTGGTGTGCTGATCTGCAAAC CCTGGACACAATGCAGCCAATTGAGAGGAAAAGACAGGGCTATATTCATGAGCTGATTCA GACCGAAGAGCGGTACATGGCTGACCTTCAGCTCGTCGTCGAGGTTTTTCAGAAACGCAT GGCAGAGTCAGGCTTTCTCACTGAAGGGGAGATGGCCCTGATTTTTGTTAACTGGAAGGA GCTCATCATGTCCAACACAAAGCTGCTGAAGGCTTTGCGGGTGCGGAAGAAGACCGGGGG CGAGAAGATGCCGGTGCAGATGATTGGGGACATCCTGGCCGCTGAGCTGTCCCACATGCA GGCTTACATCAGGTTCTGCAGCTGCCAGCTTAATGGAGCAGCTCTGTTACAGCAGAAGAC AGATGAAGACACAGATTTCAAAGAATTTTTAAAGAAGCTGGCATCTGACCCGCGGTGTAA AGGAATGCCCCTCTCCAGCTTCCTGCTGAAACCCATGCAGAGGATCACCCGCTACCCACT GCTCATCAGAAGTATTCTGGAGAACACCCCGGAGAGCCATGCAGACCATTCCTCCCTAAA GCTGGCCCTCGAGCGGGCAGAGGAGCTGTGCTCTCAAGTGAATGAGGGAGTTCGGGAGAA GGAAAACTCGGACCGACTGGAGTGGATCCAGGCGCACGTGCAGTGTGAAGGCCTCGCGGA GCAACTTATTTTCAACTCTCTCACCAACTGCCTGGGGCCCCGGAAGCTCTTACACAGTGG GAAATTATACAAGACCAAGAGCAACAAGGAACTGCACGGATTCCTCTTCAATGACTTCCT GCTTCTTACCTACATGGTCAAGCAGTTTGCTGTTTCCTCTGGCTCTGAGAAACTTTTCAG CTCGAAGTCCAATGCTCAATTCAAAATGTATAAAACGCCCATTTTCCTGAATGAAGTCTT GGTGAAACTGCCCACAGACCCTTCCAGCGATGAGCCTGTCTTCCACATTTCCCACATTGA TCGGGTCTACACCCTCCGAACAGACAACATTAATGAGAGGACCGCCTGGGTGCAGAAGAT CAAGGCGGCGTCTGAGCAGTACATCGACACCGAGAAGAAGCAGCGTGAGAAAGCTTACCA AGCCCGCTCCCAAAAGACTTCAGGCATTGGGCGCCTGATGGTGCATGTCATTGAAGCTAC AGAATTAAAAGCCTGCAAACCAAATGGAAAGAGCAACCCATACTGTGAAATCAGCATGGG CTCCCAGAGCTACACCACCAGGACCATCCAGGACACACTCAATCCCAAGTGGAATTTTAA CTGCCAGTTCTTTATTAAGGATCTCTACCAAGACGTGCTGTGTCTCACCCTGTTTGACAG AGACCAGTTTTCACCAGATGATTTCCTGGGTCGTACTGAAATTCCAGTGGCAAAAATTCG AACAGAACAGGAAAGCAAAGGCCCTATGACCCGCCGACTGCTGCTGCATGAGGTCCCCAC CGGGGAGGTCTGGGTCCGTTTTGACCTGCAGCTTTTTGAGCAAAAAACTCTCCTGGTTTA AACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTG ATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAAT AACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAG TTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGAT GCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGC ATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAAC CTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTT GTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCT TTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACC CCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCC TGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTC GCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTG GTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGAT CTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGC ACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAA CTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAA AAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGT GATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCT TTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAAT GAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTG CGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGG ATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTT ATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGG CCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATG GATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTC GAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGT TATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGG CCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACG AGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGAT ACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTA CCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCT GTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCC CCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA GACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATG TAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAG TATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTT GATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTA CGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTC AGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCA CCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGA TGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTAC CCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTT TCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTA GCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATC GAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT
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