RAP1GAP ORF clone FHC00086

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC00086

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Clone info.

Species

human

Clone Name

pFN21AA0474 Sequence (with vector) Restriction Map

Product ID

FHC00086

Flexi Clone PID

FXC00086

ORF Sequence

DNA sequence (2043 bp)
Protein sequence (681 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB384486]

Gene

KIAA0474

Symbol

RAP1GAP

Alias : RAP1GA1, RAP1GAP1, RAP1GAPII, RAPGAP

Description

RAP1 GTPase activating protein, transcript variant 2

Original Clone ID

fj12051

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 2043 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 681 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS53276.1 1.6e-218 100.0 RAP1GAP CCDS81276.1 2.9e-161 91.7 RAP1GAP CCDS218.1 4e-159 95.1 RAP1GAP CCDS53277.1 4.3e-159 95.1 RAP1GAP CCDS82035.1 1.2e-82 54.3 RAP1GAP2 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR003109 1 17 PF02188 GoLoco motif IPR000331 210 396 PF02145 Rap/ran-GAP HMMSmart IPR003109 1 17 SM00390 GoLoco motif ProfileScan IPR003109 1 17 PS50877 GoLoco motif IPR000331 181 397 PS50085 Rap/ran-GAP
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) XP_016857473 681 600278 100.0 100.0 XP_005246012 681 600278 100.0 100.0 XP_016857459 681 600278 100.0 100.0 NP_001139129 681 600278 100.0 100.0 XP_016857458 689 600278 98.8 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AA0474 Download

>KIAA0474  2043 bp
ATGATTGAGAAGATGCAGGGAAGCAGGATGGATGAACAACGCTGCTCCTTCCCGCCGCCC
CTCAAAACAGAGGAGGACTACATTCCATACCCGAGCGTGCACGAGGTCTTGGGGCGAGAA
GGACCCTTCCCCCTCATCCTGCTGCCCCAGTTTGGGGGCTACTGGATTGAGGGCACCAAC
CACGAAATCACCAGCATCCCCGAGACAGAGCCACTGCAGTCGCCCACAACCAAGGTGAAG
CTCGAGTGCAACCCCACAGCCCGCATCTACCGGAAGCACTTTCTCGGCAAGGAGCATTTC
AATTACTACTCACTGGACGCTGCCCTCGGCCACCTTGTCTTCTCACTCAAGTACGATGTC
ATCGGGGACCAAGAGCACCTGCGGCTGCTGCTCAGGACCAAGTGCCGGACATACCATGAT
GTCATCCCCATCTCCTGCCTCACCGAGTTCCCTAATGTTGTCCAGATGGCAAAGTTGGTG
TGTGAAGACGTCAATGTGGATCGGTTCTATCCTGTGCTCTACCCCAAGGCTTCCCGGCTC
ATCGTCACCTTTGACGAGCATGTCATCAGCAATAACTTCAAGTTTGGCGTCATTTATCAG
AAGCTTGGGCAGACCTCCGAGGAAGAACTCTTCAGCACCAATGAGGAAAGTCCCGCTTTC
GTGGAGTTCCTTGAATTTCTTGGCCAGAAGGTCAAACTGCAGGACTTTAAGGGGTTCCGA
GGAGGCCTGGACGTGACCCACGGGCAGACGGGGACCGAATCTGTGTACTGCAACTTCCGC
AACAAGGAGATCATGTTTCACGTGTCCACCAAGCTGCCATACACGGAAGGGGACGCCCAG
CAGTTGCAGCGGAAGCGGCACATCGGGAACGACATCGTGGCTGTGGTCTTCCAGGATGAG
AACACTCCTTTCGTGCCCGACATGATCGCGTCCAACTTCCTGCATGCCTACGTCGTGGTG
CAGGCTGAGGGCGGGGGCCCTGATGGCCCCCTCTACAAGGTCTCTGTCACTGCAAGAGAT
GATGTGCCCTTCTTTGGACCCCCCCTCCCGGACCCCGCTGTGTTCAGGAAGGGGCCTGAG
TTCCAGGAATTTTTGCTGACAAAGCTGATCAATGCTGAATATGCCTGCTACAAGGCAGAG
AAGTTTGCCAAACTGGAGGAGCGGACGCGGGCCGCCCTCCTGGAGACGCTCTATGAGGAA
CTACACATCCACAGCCAGTCCATGATGGGCTTGGGCGGCGACGAGGACAAGATGGAGAAT
GGCAGTGGGGGCGGCGGCTTCTTTGAGTCTTTCAAGCGGGTCATCCGGAGCCGCAGCCAG
TCCATGGATGCCATGGGGCTGAGCAACAAGAAGCCCAACACCGTGTCCACCAGCCACAGC
GGGAGCTTCGCGCCCAACAACCCCGACCTGGCCAAGGCGGCTGGAATATCATTGCTTATT
CCTGGGAAAAGTGCGAGTAGATTCGGACGCCGGGGCAGTGCCATAGGCATAGGAACCGTG
GAAGAGTCACTGATTGTCCCTGGGAAGAGCCCCACGAGGAAGAAGTCGGGCCCGTTCGGC
TCCCGCCGCAGCAGCGCCATTGGCATCGAGAACATACAGGAGGTGCAGGAGAAGAGGGAG
AGCCCTCCGGCTGGTCAGAAGACCCCAGACAGCGGGCACGTCTCACAGGAGCCCAAGTCG
GAGAACTCATCCACTCAGAGCTCCCCAGAGATGCCCACGACCAAGAACAGAGCGGAGACC
GCAGCGCAGAGAGCAGAGGCGCTCAAGGACTTCTCCCGCTCCTCGTCCAGTGCCAGCAGC
TTCGCCAGCGTGGTGGAGGAGACGGAGGGTGTGGACGGAGAGGACACAGGCCTGGAGAGC
GTGTCATCCTCAGGAACACCCCACAAGCGGGACTCCTTCATCTATAGCACGTGGCTGGAG
GACAGTGTCAGCACCACTAGTGGGGGCAGCTCCCCAGACGCCGGCAAGTTGGGGGACCCT
GCGTGTCCCGAGATCAAGATCCAGCTGGAAGCATCTGAGCAGCACATGCCCCAGCTGGGC
TGT

ORF Protein

Cloned ORF protein sequence for pFN21AA0474 Download

>KIAA0474  681 aa
MIEKMQGSRMDEQRCSFPPPLKTEEDYIPYPSVHEVLGREGPFPLILLPQFGGYWIEGTN
HEITSIPETEPLQSPTTKVKLECNPTARIYRKHFLGKEHFNYYSLDAALGHLVFSLKYDV
IGDQEHLRLLLRTKCRTYHDVIPISCLTEFPNVVQMAKLVCEDVNVDRFYPVLYPKASRL
IVTFDEHVISNNFKFGVIYQKLGQTSEEELFSTNEESPAFVEFLEFLGQKVKLQDFKGFR
GGLDVTHGQTGTESVYCNFRNKEIMFHVSTKLPYTEGDAQQLQRKRHIGNDIVAVVFQDE
NTPFVPDMIASNFLHAYVVVQAEGGGPDGPLYKVSVTARDDVPFFGPPLPDPAVFRKGPE
FQEFLLTKLINAEYACYKAEKFAKLEERTRAALLETLYEELHIHSQSMMGLGGDEDKMEN
GSGGGGFFESFKRVIRSRSQSMDAMGLSNKKPNTVSTSHSGSFAPNNPDLAKAAGISLLI
PGKSASRFGRRGSAIGIGTVEESLIVPGKSPTRKKSGPFGSRRSSAIGIENIQEVQEKRE
SPPAGQKTPDSGHVSQEPKSENSSTQSSPEMPTTKNRAETAAQRAEALKDFSRSSSSASS
FASVVEETEGVDGEDTGLESVSSSGTPHKRDSFIYSTWLEDSVSTTSGGSSPDAGKLGDP
ACPEIKIQLEASEQHMPQLGC

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AA0474 Download

>pFN21AA0474  6748 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGATTGAGAAGATGCAGGGAAGCAGGATGGATGA
ACAACGCTGCTCCTTCCCGCCGCCCCTCAAAACAGAGGAGGACTACATTCCATACCCGAG
CGTGCACGAGGTCTTGGGGCGAGAAGGACCCTTCCCCCTCATCCTGCTGCCCCAGTTTGG
GGGCTACTGGATTGAGGGCACCAACCACGAAATCACCAGCATCCCCGAGACAGAGCCACT
GCAGTCGCCCACAACCAAGGTGAAGCTCGAGTGCAACCCCACAGCCCGCATCTACCGGAA
GCACTTTCTCGGCAAGGAGCATTTCAATTACTACTCACTGGACGCTGCCCTCGGCCACCT
TGTCTTCTCACTCAAGTACGATGTCATCGGGGACCAAGAGCACCTGCGGCTGCTGCTCAG
GACCAAGTGCCGGACATACCATGATGTCATCCCCATCTCCTGCCTCACCGAGTTCCCTAA
TGTTGTCCAGATGGCAAAGTTGGTGTGTGAAGACGTCAATGTGGATCGGTTCTATCCTGT
GCTCTACCCCAAGGCTTCCCGGCTCATCGTCACCTTTGACGAGCATGTCATCAGCAATAA
CTTCAAGTTTGGCGTCATTTATCAGAAGCTTGGGCAGACCTCCGAGGAAGAACTCTTCAG
CACCAATGAGGAAAGTCCCGCTTTCGTGGAGTTCCTTGAATTTCTTGGCCAGAAGGTCAA
ACTGCAGGACTTTAAGGGGTTCCGAGGAGGCCTGGACGTGACCCACGGGCAGACGGGGAC
CGAATCTGTGTACTGCAACTTCCGCAACAAGGAGATCATGTTTCACGTGTCCACCAAGCT
GCCATACACGGAAGGGGACGCCCAGCAGTTGCAGCGGAAGCGGCACATCGGGAACGACAT
CGTGGCTGTGGTCTTCCAGGATGAGAACACTCCTTTCGTGCCCGACATGATCGCGTCCAA
CTTCCTGCATGCCTACGTCGTGGTGCAGGCTGAGGGCGGGGGCCCTGATGGCCCCCTCTA
CAAGGTCTCTGTCACTGCAAGAGATGATGTGCCCTTCTTTGGACCCCCCCTCCCGGACCC
CGCTGTGTTCAGGAAGGGGCCTGAGTTCCAGGAATTTTTGCTGACAAAGCTGATCAATGC
TGAATATGCCTGCTACAAGGCAGAGAAGTTTGCCAAACTGGAGGAGCGGACGCGGGCCGC
CCTCCTGGAGACGCTCTATGAGGAACTACACATCCACAGCCAGTCCATGATGGGCTTGGG
CGGCGACGAGGACAAGATGGAGAATGGCAGTGGGGGCGGCGGCTTCTTTGAGTCTTTCAA
GCGGGTCATCCGGAGCCGCAGCCAGTCCATGGATGCCATGGGGCTGAGCAACAAGAAGCC
CAACACCGTGTCCACCAGCCACAGCGGGAGCTTCGCGCCCAACAACCCCGACCTGGCCAA
GGCGGCTGGAATATCATTGCTTATTCCTGGGAAAAGTGCGAGTAGATTCGGACGCCGGGG
CAGTGCCATAGGCATAGGAACCGTGGAAGAGTCACTGATTGTCCCTGGGAAGAGCCCCAC
GAGGAAGAAGTCGGGCCCGTTCGGCTCCCGCCGCAGCAGCGCCATTGGCATCGAGAACAT
ACAGGAGGTGCAGGAGAAGAGGGAGAGCCCTCCGGCTGGTCAGAAGACCCCAGACAGCGG
GCACGTCTCACAGGAGCCCAAGTCGGAGAACTCATCCACTCAGAGCTCCCCAGAGATGCC
CACGACCAAGAACAGAGCGGAGACCGCAGCGCAGAGAGCAGAGGCGCTCAAGGACTTCTC
CCGCTCCTCGTCCAGTGCCAGCAGCTTCGCCAGCGTGGTGGAGGAGACGGAGGGTGTGGA
CGGAGAGGACACAGGCCTGGAGAGCGTGTCATCCTCAGGAACACCCCACAAGCGGGACTC
CTTCATCTATAGCACGTGGCTGGAGGACAGTGTCAGCACCACTAGTGGGGGCAGCTCCCC
AGACGCCGGCAAGTTGGGGGACCCTGCGTGTCCCGAGATCAAGATCCAGCTGGAAGCATC
TGAGCAGCACATGCCCCAGCTGGGCTGTGTTTAAACGAATTCGGGCTCGGTACCCGGGGA
TCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGG
AAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCG
CTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAG
TGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATA
AGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGG
GAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTC
TAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTC
ATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCA
GGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACAT
TCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAA
AGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTT
TGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAG
TTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGT
TTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCG
GTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAG
AATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTA
AGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTG
ACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTA
ACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGAC
ACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTT
ACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCA
CTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAG
CGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTA
GTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAG
ATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAAC
CGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAG
GAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGC
TGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTC
AGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCC
TCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTT
CGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCG
TTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTAT
CCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAG
CCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGT
GGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGC
CAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTA
GCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAG
ATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGA
TTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATA
CAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTT
CAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGG
TTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCT
GTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGG
TAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACG
ATGCGTCCGGCGTAGAGGATCGAGATCT