ALG3 ORF clone FHC09815

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC09815

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Clone info.

Species

human

Clone Name

pFN21AE6324 Sequence (with vector) Restriction Map

Product ID

FHC09815

ORF Sequence

DNA sequence (1314 bp)
Protein sequence (438 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE6324

Symbol

ALG3

Alias : CDG1D, CDGS4, CDGS6, D16Ertd36e, NOT56L, Not56, not

Description

ALG3, alpha-1,3- mannosyltransferase, transcript variant 1

Original Clone ID

oc96324

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1314 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 438 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS46968.1 7.7e-210 100.0 ALG3 CCDS46967.1 2e-177 100.0 ALG3 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR007873 45 406 PF05208 Glycosyltransferase
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 42 TLLVAACLCLAEVGITFWVIHRV 64 PRIMARY 23 2 95 TGPLVYPAGFVYIFMGLYYAT 115 SECONDARY 21 3 131 LYLATLLLVFLIYHQTCKVPPFV 153 PRIMARY 23 4 167 IFVLRLFNDPVAMVLLFLSINLL 189 PRIMARY 23 5 200 FFSLAVSVKMNVLLFAPGLLFLL 222 PRIMARY 23 6 233 PKLGICAGLQVVLGLPFLLENPS 255 SECONDARY 23 7 288 AFHLALLTAHLTLLLLFALCRWH 310 PRIMARY 23 8 377 ARWLTHLLRLLVLGLIELSWNT 398 SECONDARY 22
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_005778 438 601110,608750 100.0 100.0 XP_011510625 398 601110,608750 99.5 85.6 NP_001006942 390 601110,608750 100.0 84.9 XP_011510624 405 601110,608750 100.0 84.9 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE6324 Download

>KIEE6324  1314 bp
ATGGCGGCTGGGCTGCGGAAACGCGGCCGGTCCGGTTCCGCGGCCCAGGCAGAGGGACTC
TGCAAGCAATGGCTGCAGCGCGCCTGGCAAGAGCGGCGCCTGCTGCTGCGGGAGCCGCGC
TACACGCTGCTGGTGGCCGCCTGCCTCTGCCTGGCGGAGGTGGGCATCACCTTCTGGGTC
ATTCACAGGGTGGCATACACAGAGATTGACTGGAAGGCCTACATGGCCGAGGTAGAAGGC
GTCATCAATGGTACCTATGACTATACCCAACTGCAGGGTGACACCGGACCACTTGTGTAC
CCAGCTGGTTTCGTGTACATCTTTATGGGGTTGTACTATGCCACCAGCCGAGGCACTGAC
ATCCGCATGGCCCAGAACATCTTTGCTGTGCTCTACCTGGCTACCTTGCTGCTTGTCTTC
TTGATCTATCACCAGACCTGCAAGGTACCTCCCTTCGTCTTTTTCTTCATGTGCTGCGCC
TCTTACCGTGTCCACTCCATCTTTGTGCTGCGGCTCTTCAATGACCCAGTGGCCATGGTG
CTGCTCTTCCTCAGTATCAACCTCCTGCTGGCCCAGCGCTGGGGCTGGGGTTGCTGCTTT
TTCAGCCTGGCAGTCTCTGTGAAGATGAATGTGCTGCTCTTCGCCCCTGGGTTACTGTTT
CTTCTCCTCACACAGTTTGGCTTCCGTGGGGCCCTCCCCAAGCTGGGAATCTGTGCTGGC
CTTCAGGTGGTGCTGGGGCTGCCCTTCCTGCTGGAGAACCCCAGCGGCTACCTGTCCCGC
TCCTTTGACCTTGGCCGCCAGTTTCTGTTCCACTGGACAGTGAACTGGCGCTTCCTCCCA
GAGGCGCTCTTCCTGCATCGAGCCTTCCACCTGGCCCTGTTGACTGCCCACCTCACCCTG
CTCCTGCTGTTTGCCCTCTGCAGGTGGCACAGGACAGGGGAAAGTATCTTGTCGCTGCTG
AGGGATCCCTCCAAAAGGAAGGTTCCACCCCAGCCCCTTACACCCAACCAGATCGTTTCT
ACCCTCTTCACCTCCAACTTCATTGGCATCTGCTTCAGCCGCTCCCTCCACTACCAGTTC
TACGTCTGGTATTTCCACACACTGCCCTACCTCCTGTGGGCCATGCCTGCACGCTGGCTC
ACACACCTGCTCAGGTTGTTGGTGCTGGGGCTCATCGAGCTCTCCTGGAACACATACCCT
TCCACATCCTGCAGCTCTGCTGCCCTGCACATATGCCATGCCGTCATCCTGCTGCAGCTC
TGGCTGGGCCCGCAGCCTTTCCCCAAGAGCACCCAACACAGCAAGAAAGCCCAC

ORF Protein

Cloned ORF protein sequence for pFN21AE6324 Download

>KIEE6324  438 aa
MAAGLRKRGRSGSAAQAEGLCKQWLQRAWQERRLLLREPRYTLLVAACLCLAEVGITFWV
IHRVAYTEIDWKAYMAEVEGVINGTYDYTQLQGDTGPLVYPAGFVYIFMGLYYATSRGTD
IRMAQNIFAVLYLATLLLVFLIYHQTCKVPPFVFFFMCCASYRVHSIFVLRLFNDPVAMV
LLFLSINLLLAQRWGWGCCFFSLAVSVKMNVLLFAPGLLFLLLTQFGFRGALPKLGICAG
LQVVLGLPFLLENPSGYLSRSFDLGRQFLFHWTVNWRFLPEALFLHRAFHLALLTAHLTL
LLLFALCRWHRTGESILSLLRDPSKRKVPPQPLTPNQIVSTLFTSNFIGICFSRSLHYQF
YVWYFHTLPYLLWAMPARWLTHLLRLLVLGLIELSWNTYPSTSCSSAALHICHAVILLQL
WLGPQPFPKSTQHSKKAH

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE6324 Download

>pFN21AE6324  6019 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCGGCTGGGCTGCGGAAACGCGGCCGGTCCGG
TTCCGCGGCCCAGGCAGAGGGACTCTGCAAGCAATGGCTGCAGCGCGCCTGGCAAGAGCG
GCGCCTGCTGCTGCGGGAGCCGCGCTACACGCTGCTGGTGGCCGCCTGCCTCTGCCTGGC
GGAGGTGGGCATCACCTTCTGGGTCATTCACAGGGTGGCATACACAGAGATTGACTGGAA
GGCCTACATGGCCGAGGTAGAAGGCGTCATCAATGGTACCTATGACTATACCCAACTGCA
GGGTGACACCGGACCACTTGTGTACCCAGCTGGTTTCGTGTACATCTTTATGGGGTTGTA
CTATGCCACCAGCCGAGGCACTGACATCCGCATGGCCCAGAACATCTTTGCTGTGCTCTA
CCTGGCTACCTTGCTGCTTGTCTTCTTGATCTATCACCAGACCTGCAAGGTACCTCCCTT
CGTCTTTTTCTTCATGTGCTGCGCCTCTTACCGTGTCCACTCCATCTTTGTGCTGCGGCT
CTTCAATGACCCAGTGGCCATGGTGCTGCTCTTCCTCAGTATCAACCTCCTGCTGGCCCA
GCGCTGGGGCTGGGGTTGCTGCTTTTTCAGCCTGGCAGTCTCTGTGAAGATGAATGTGCT
GCTCTTCGCCCCTGGGTTACTGTTTCTTCTCCTCACACAGTTTGGCTTCCGTGGGGCCCT
CCCCAAGCTGGGAATCTGTGCTGGCCTTCAGGTGGTGCTGGGGCTGCCCTTCCTGCTGGA
GAACCCCAGCGGCTACCTGTCCCGCTCCTTTGACCTTGGCCGCCAGTTTCTGTTCCACTG
GACAGTGAACTGGCGCTTCCTCCCAGAGGCGCTCTTCCTGCATCGAGCCTTCCACCTGGC
CCTGTTGACTGCCCACCTCACCCTGCTCCTGCTGTTTGCCCTCTGCAGGTGGCACAGGAC
AGGGGAAAGTATCTTGTCGCTGCTGAGGGATCCCTCCAAAAGGAAGGTTCCACCCCAGCC
CCTTACACCCAACCAGATCGTTTCTACCCTCTTCACCTCCAACTTCATTGGCATCTGCTT
CAGCCGCTCCCTCCACTACCAGTTCTACGTCTGGTATTTCCACACACTGCCCTACCTCCT
GTGGGCCATGCCTGCACGCTGGCTCACACACCTGCTCAGGTTGTTGGTGCTGGGGCTCAT
CGAGCTCTCCTGGAACACATACCCTTCCACATCCTGCAGCTCTGCTGCCCTGCACATATG
CCATGCCGTCATCCTGCTGCAGCTCTGGCTGGGCCCGCAGCCTTTCCCCAAGAGCACCCA
ACACAGCAAGAAAGCCCACGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGA
GTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGT
TGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCA
GACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAA
TGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAAT
AAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGG
GAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATC
CTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGT
CAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACT
TTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATG
TATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT
ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCT
GTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCA
CGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCC
GAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCC
CGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTG
GTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTA
TGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATC
GGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTT
GATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATG
CCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCT
TCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGC
TCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCT
CGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTAC
ACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCC
TCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAG
CTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACA
TGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTT
TCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGC
GAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCT
CTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCG
TGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCA
AGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACT
ATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTA
ACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTA
ACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCT
TCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTT
TTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGA
TCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCA
TGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGC
ACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATT
AAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTT
ATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGC
CGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCAC
TTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCG
GCGTAGAGGATCGAGATCT