(Circular) (Six-base) MAPSORT of: pFN21AE6324.seq Check: 6593 from: 1 to: 6019 pFN21AE6324 6019 bp With 182 enzymes: SgfI * March 18, 2015 14:50 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2916 3159 2916 Size: 243 5776 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3829 278 Size: 53 83 186 936 2293 2468 Fragments arranged by size: 2468 2293 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3362 1937 Size: 1425 4594 Acc65I G'GTAC_C Cuts at: 2255 2449 3340 2255 Size: 194 891 4934 Fragments arranged by size: 4934 891 194 AclI AA'CG_TT Cuts at: 1563 4146 4519 1563 Size: 2583 373 3063 Fragments arranged by size: 3063 2583 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2711 3043 4082 5466 5734 1632 Size: 1079 332 1039 1384 268 1917 Fragments arranged by size: 1917 1384 1079 1039 332 268 AfeI AGC'GCT Cuts at: 2582 2582 Size: 6019 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5822 AflIII A'CryG_T Cuts at: 1203 4918 5895 1203 Size: 3715 977 1327 Fragments arranged by size: 3715 1327 977 AgeI A'CCGG_T Cuts at: 4850 4850 Size: 6019 AhdI GACnn_n'nnGTC Cuts at: 4748 4748 Size: 6019 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6019 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6019 AlwNI CAG_nnn'CTG Cuts at: 2621 2809 3762 5334 2621 Size: 188 953 1572 3306 Fragments arranged by size: 3306 1572 953 188 ApaI G_GGCC'C Cuts at: 2698 3275 2698 Size: 577 5442 ApaLI G'TGCA_C Cuts at: 4076 5232 4076 Size: 1156 4863 ApoI r'AATT_y Cuts at: 1493 3328 3554 3706 5815 1493 Size: 1835 226 152 2109 1697 Fragments arranged by size: 2109 1835 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4573 160 Size: 872 3541 1606 Fragments arranged by size: 3541 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6019 AvaI C'yCGr_G Cuts at: 1870 1943 3344 1870 Size: 73 1401 4545 Fragments arranged by size: 4545 1401 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2285 2318 2285 Size: 33 5986 BamHI G'GATC_C Cuts at: 2968 3349 3716 2968 Size: 381 367 5271 Fragments arranged by size: 5271 381 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2100 2255 2449 3340 4795 Size: 325 217 627 313 155 194 891 1455 Cuts at: 4795 618 Size: 1842 Fragments arranged by size: 1842 1455 891 627 325 313 217 194 155 BanII G_rGCy'C Cuts at: 1391 2698 3177 3186 3275 3338 1391 Size: 1307 479 9 89 63 4072 Fragments arranged by size: 4072 1307 479 89 63 9 BbeI G_GCGC'C Cuts at: 2104 2104 Size: 6019 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2413 928 Size: 971 514 4534 Fragments arranged by size: 4534 971 514 BbvCI CC'TCA_GC Cuts at: 2963 2963 Size: 6019 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4210 4244 1903 Size: 34 2273 34 3678 Fragments arranged by size: 3678 2273 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3911 5121 3911 Size: 1210 4809 BclI T'GATC_A Cuts at: 1548 1548 Size: 6019 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2228 4630 Size: 107 122 71 631 605 337 219 2402 Cuts at: 4630 136 Size: 1525 Fragments arranged by size: 2402 1525 631 605 337 219 122 107 71 BglII A'GATC_T Cuts at: 6014 6014 Size: 6019 BlpI GC'TnA_GC Cuts at: 3436 3436 Size: 6019 Bme1580I G_kGCm'C Cuts at: 1790 2698 3275 4080 5236 1790 Size: 908 577 805 1156 2573 Fragments arranged by size: 2573 1156 908 805 577 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6019 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2525 3750 4708 Size: 416 53 83 399 24 1108 1225 958 Cuts at: 4708 442 Size: 1753 Fragments arranged by size: 1753 1225 1108 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6019 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2777 4679 1239 Size: 1538 1902 2579 Fragments arranged by size: 2579 1902 1538 Bpu10I CC'TnA_GC Cuts at: 2963 3155 2963 Size: 192 5827 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4144 5009 5307 4144 Size: 865 298 4856 Fragments arranged by size: 4856 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4682 882 Size: 617 3183 2219 Fragments arranged by size: 3183 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3810 493 Size: 711 2606 2702 Fragments arranged by size: 2702 2606 711 BsaBI GATnn'nnATC Cuts at: 6013 6013 Size: 6019 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2101 2244 3826 4208 Size: 53 83 186 936 568 143 1582 382 Cuts at: 4208 4843 275 Size: 635 1451 Fragments arranged by size: 1582 1451 936 635 568 382 186 143 83 53 BsaWI w'CCGG_w Cuts at: 2036 2288 4452 4850 5124 5271 5682 2036 Size: 252 2164 398 274 147 411 2373 Fragments arranged by size: 2373 2164 411 398 274 252 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5794 Fragments arranged by size: 5794 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2660 1186 Size: 1474 4545 BseYI C'CCAG_C Cuts at: 2012 2305 2577 2583 2589 2707 2737 2765 2994 Size: 293 272 6 6 118 30 28 229 Cuts at: 2994 3169 3268 5222 5718 2012 Size: 175 99 1954 496 2313 Fragments arranged by size: 2313 1954 496 293 272 229 175 118 99 30 28 6 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3117 3215 1163 Size: 573 1381 98 3967 Fragments arranged by size: 3967 1381 573 98 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2032 3469 4230 5258 1540 Size: 275 186 31 1437 761 1028 2301 Fragments arranged by size: 2301 1437 1028 761 275 186 31 BsiHKAI G_wGCw'C Cuts at: 2397 3186 3296 4080 4165 5236 2397 Size: 789 110 784 85 1071 3180 Fragments arranged by size: 3180 1071 789 784 110 85 BsmI GAATG_Cn' Cuts at: 3530 3623 3530 Size: 93 5926 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2397 2698 3177 3186 3275 3296 3338 Size: 399 607 301 479 9 89 21 42 Cuts at: 3338 4080 4165 5236 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 607 479 399 301 89 85 42 21 9 BspEI T'CCGG_A Cuts at: 5682 5682 Size: 6019 BspHI T'CATG_A Cuts at: 3908 5638 3908 Size: 1730 4289 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2450 2916 3159 3374 844 Size: 1606 466 243 215 3489 Fragments arranged by size: 3489 1606 466 243 215 BsrBI CCG'CTC Cuts at: 1394 1823 2098 2784 3066 3906 1394 Size: 429 275 686 282 840 3507 Fragments arranged by size: 3507 840 686 429 282 275 BsrDI GCAATG_nn' Cuts at: 65 1248 2078 4514 4688 65 Size: 1183 830 2436 174 1396 Fragments arranged by size: 2436 1396 1183 830 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2031 4663 4850 1700 Size: 110 221 2632 187 2869 Fragments arranged by size: 2869 2632 221 187 110 BsrGI T'GTAC_A Cuts at: 96 2319 96 Size: 2223 3796 BssHII G'CGCG_C Cuts at: 1925 2083 1925 Size: 158 5861 BssSI C'ACGA_G Cuts at: 4079 5091 4079 Size: 1012 5007 BstAPI GCAn_nnn'nTGC Cuts at: 794 2077 794 Size: 1283 4736 BstBI TT'CG_AA Cuts at: 4823 4823 Size: 6019 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6019 BstYI r'GATC_y Cuts at: 1972 2968 3349 3716 4101 4118 5570 5656 5668 Size: 996 381 367 385 17 1452 86 12 Cuts at: 5668 6014 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 996 385 381 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2043 2538 2689 513 Size: 552 939 39 495 151 3843 Fragments arranged by size: 3843 939 552 495 151 39 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5280 Fragments arranged by size: 5280 683 56 BtsI GCAGTG_nn' Cuts at: 3104 3535 4329 4349 3104 Size: 431 794 20 4774 Fragments arranged by size: 4774 794 431 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6019 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5984 DraI TTT'AAA Cuts at: 3323 3323 Size: 6019 DraIII CAC_nnn'GTG Cuts at: 2194 2194 Size: 6019 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5026 809 Size: 507 3710 1802 Fragments arranged by size: 3710 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2029 2139 2229 Size: 54 1425 50 275 18 199 110 90 Cuts at: 2229 2535 2797 3466 4355 5984 8 Size: 306 262 669 889 1629 43 Fragments arranged by size: 1629 1425 889 669 306 275 262 199 110 90 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2029 3466 1537 Size: 275 217 1437 4090 Fragments arranged by size: 4090 1437 275 217 EarI CTCTTCn'nnn_ Cuts at: 1353 2525 2555 2648 2858 3035 3949 1353 Size: 1172 30 93 210 177 914 3423 Fragments arranged by size: 3423 1172 914 210 177 93 30 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2174 4606 4980 5126 387 Size: 1064 138 585 2432 374 146 1280 Fragments arranged by size: 2432 1280 1064 585 374 146 138 EcoICRI GAG'CTC Cuts at: 3184 3184 Size: 6019 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2711 2777 3043 4082 4679 5466 5734 Size: 393 1079 66 266 1039 597 787 268 Cuts at: 5734 1239 Size: 1524 Fragments arranged by size: 1524 1079 1039 787 597 393 268 266 66 EcoNI CCTnn'n_nnAGG Cuts at: 2159 2843 2977 2159 Size: 684 134 5201 Fragments arranged by size: 5201 684 134 EcoO109I rG'GnC_Cy Cuts at: 2694 2695 2694 Size: 1 6018 EcoRI G'AATT_C Cuts at: 3328 3706 3328 Size: 378 5641 FspI TGC'GCA Cuts at: 4525 4525 Size: 6019 HaeII r_GCGC'y Cuts at: 1678 2104 2584 2836 2852 5166 1678 Size: 426 480 252 16 2314 2531 Fragments arranged by size: 2531 2314 480 426 252 16 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4660 4692 4734 4766 1373 Size: 32 135 32 3088 32 42 32 2626 Fragments arranged by size: 3088 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2886 3363 3609 669 Size: 988 281 948 477 246 3079 Fragments arranged by size: 3079 988 948 477 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6019 HpaI GTT'AAC Cuts at: 3609 3609 Size: 6019 KasI G'GCGC_C Cuts at: 2100 2100 Size: 6019 KpnI G_GTAC'C Cuts at: 2259 2453 3344 2259 Size: 194 891 4934 Fragments arranged by size: 4934 891 194 MfeI C'AATT_G Cuts at: 3618 3618 Size: 6019 MluI A'CGCG_T Cuts at: 5895 5895 Size: 6019 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3063 5133 5317 1305 Size: 1758 2070 184 2007 Fragments arranged by size: 2070 2007 1758 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2537 10 Size: 54 1425 343 705 3492 Fragments arranged by size: 3492 1425 705 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2327 2369 2507 3977 4336 4495 518 Size: 648 1161 42 138 1470 359 159 2042 Fragments arranged by size: 2042 1470 1161 648 359 159 138 42 MspA1I CmG'CkG Cuts at: 1606 2045 2309 2769 3435 4112 5260 5505 1606 Size: 439 264 460 666 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 666 460 439 264 245 NarI GG'CG_CC Cuts at: 2101 2101 Size: 6019 NcoI C'CATG_G Cuts at: 513 1065 2004 2538 513 Size: 552 939 534 3994 Fragments arranged by size: 3994 939 552 534 NdeI CA'TA_TG Cuts at: 387 3236 387 Size: 2849 3170 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6019 NotI GC'GGCC_GC Cuts at: 3466 3466 Size: 6019 NspI r_CATG'y Cuts at: 1132 1229 3377 4922 1132 Size: 97 2148 1545 2229 Fragments arranged by size: 2229 2148 1545 97 PasI CC'CwG_GG Cuts at: 1348 2651 1348 Size: 1303 4716 PciI A'CATG_T Cuts at: 4918 4918 Size: 6019 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6019 PfoI T'CCnGG_A Cuts at: 3188 3188 Size: 6019 PmeI GTTT'AAAC Cuts at: 3323 3323 Size: 6019 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4088 4120 5601 5633 4088 Size: 32 1481 32 4474 Fragments arranged by size: 4474 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6019 PsiI TTA'TAA Cuts at: 3589 3589 Size: 6019 PspOMI G'GGCC_C Cuts at: 2694 3271 2694 Size: 577 5442 PstI C_TGCA'G Cuts at: 830 2082 2280 2927 3218 3261 3371 830 Size: 1252 198 647 291 43 110 3478 Fragments arranged by size: 3478 1252 647 291 198 110 43 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6019 PvuII CAG'CTG Cuts at: 2309 2309 Size: 6019 RsrII CG'GwC_CG Cuts at: 2034 2034 Size: 6019 SacI G_AGCT'C Cuts at: 3186 3186 Size: 6019 SacII CC_GC'GG Cuts at: 2046 2046 Size: 6019 SalI G'TCGA_C Cuts at: 1936 3361 1936 Size: 1425 4594 SapI GCTCTTCn'nnn_ Cuts at: 2525 2555 2648 2858 2525 Size: 30 93 210 5686 Fragments arranged by size: 5686 210 93 30 SbfI CC_TGCA'GG Cuts at: 3371 3371 Size: 6019 ScaI AGT'ACT Cuts at: 4267 5979 4267 Size: 1712 4307 SfcI C'TryA_G Cuts at: 826 1045 2078 2276 2923 3214 3257 3367 4502 Size: 219 1033 198 647 291 43 110 1135 Cuts at: 4502 5183 5374 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1033 681 647 291 219 198 191 110 43 SfiI GGCCn_nnn'nGGCC Cuts at: 2228 2228 Size: 6019 SfoI GGC'GCC Cuts at: 2102 2102 Size: 6019 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6019 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6019 SmaI CCC'GGG Cuts at: 1872 3346 1872 Size: 1474 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4123 5024 5286 820 Size: 197 926 2180 901 262 1553 Fragments arranged by size: 2180 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3810 493 Size: 3317 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6019 SphI G_CATG'C Cuts at: 1132 3377 1132 Size: 2245 3774 SspI AAT'ATT Cuts at: 5 52 3943 5 Size: 47 3891 2081 Fragments arranged by size: 3891 2081 47 StuI AGG'CCT Cuts at: 1517 2222 1517 Size: 705 5314 StyI C'CwwG_G Cuts at: 513 1065 2004 2538 2794 3458 513 Size: 552 939 534 256 664 3074 Fragments arranged by size: 3074 939 664 552 534 256 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3022 3311 4046 4063 4216 4401 4846 Size: 1255 1442 289 735 17 153 185 445 Cuts at: 4846 325 Size: 1498 Fragments arranged by size: 1498 1442 1255 735 445 289 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2319 2337 4265 Size: 274 80 33 51 1443 342 18 1928 Cuts at: 4265 5977 96 Size: 1712 138 Fragments arranged by size: 1928 1712 1443 342 274 138 80 51 33 18 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2283 2315 5979 6011 2283 Size: 32 3664 32 2291 Fragments arranged by size: 3664 2291 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6019 XbaI T'CTAG_A Cuts at: 3355 3355 Size: 6019 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6019 XmaI C'CCGG_G Cuts at: 1870 3344 1870 Size: 1474 4545 XmnI GAAnn'nnTTC Cuts at: 4148 4148 Size: 6019 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3827 276 Size: 53 83 186 936 2293 2468 Fragments arranged by size: 2468 2293 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI SanDI SexAI SrfI SwaI XcmI