KIF22 ORF clone FHC09248

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC09248

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Clone info.

Species

human

Clone Name

pFN21AE4553 Sequence (with vector) Restriction Map

Product ID

FHC09248

ORF Sequence

DNA sequence (1995 bp)
Protein sequence (665 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE4553

Symbol

KIF22

Alias : A-328A3.2, KID, KNSL4, OBP, OBP-1, OBP-2, SEMDJL2

Description

kinesin family member 22, transcript variant 1

Original Clone ID

oc94553

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1995 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 665 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS10653.1 4.8e-208 100.0 KIF22 CCDS58444.1 1.1e-185 100.0 KIF22 CCDS32718.2 2.8e-30 28.9 KIF19 CCDS7867.1 2.8e-30 30.8 KIF18A CCDS75296.1 5.3e-30 31.8 KIF3A The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR001752 118 139 PR00380 Kinesin IPR001752 237 254 PR00380 Kinesin IPR001752 269 287 PR00380 Kinesin IPR001752 318 339 PR00380 Kinesin HMMPfam IPR001752 51 368 PF00225 Kinesin HMMSmart IPR001752 41 376 SM00129 Kinesin IPR003583 602 621 SM00278 Helix-hairpin-helix DNA-binding motif IPR003583 632 651 SM00278 Helix-hairpin-helix DNA-binding motif ProfileScan IPR001752 40 299 PS50067 Kinesin
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_015556 665 603213,603546 100.0 100.0 NP_001243198 597 603213,603546 100.0 89.8 NP_001243199 597 603213,603546 100.0 89.8 NP_112494 898 611271 30.8 82.4 XP_016873868 898 611271 30.8 82.4 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE4553 Download

>KIEE4553  1995 bp
ATGGCCGCGGGCGGCTCGACGCAGCAGAGGCGACGCGAGATGGCGGCAGCTTCAGCGGCG
GCGATCTCAGGAGCTGGTCGCTGTCGGCTAAGCAAGATTGGAGCTACTCGTCGTCCACCT
CCAGCTCGCGTAAGGGTGGCTGTGCGACTGCGGCCATTTGTGGATGGAACAGCGGGAGCA
AGTGATCCCCCCTGTGTGCGGGGCATGGACAGCTGCTCTCTAGAGATTGCTAACTGGAGG
AACCACCAGGAGACTCTCAAATACCAGTTTGATGCCTTCTATGGGGAGAGGAGTACTCAG
CAGGACATCTATGCAGGTTCAGTGCAGCCCATCCTAAGGCACTTGCTGGAAGGGCAGAAT
GCCAGTGTGCTTGCCTATGGACCCACAGGAGCTGGGAAGACGCACACAATGCTGGGCAGC
CCAGAGCAACCTGGGGTGATCCCGCGGGCTCTCATGGACCTCCTGCAGCTCACAAGGGAG
GAGGGTGCCGAGGGCCGGCCATGGGCCCTTTCTGTCACCATGTCTTACCTAGAGATCTAC
CAGGAGAAGGTATTAGACCTCCTGGACCCTGCTTCGGGAGACCTGGTAATCCGAGAAGAC
TGCCGGGGGAATATCCTGATTCCGGGTCTCTCCCAGAAGCCCATCAGTAGCTTTGCTGAT
TTTGAGCGGCACTTCCTGCCAGCCAGTCGAAATCGGACTGTAGGAGCCACCCGGCTCAAC
CAGCGCTCCTCCCGCAGTCATGCTGTGCTCCTGGTCAAGGTGGACCAGCGGGAACGTTTG
GCCCCATTTCGCCAGCGAGAGGGAAAACTCTACCTGATTGACTTGGCTGGGTCAGAGGAC
AACCGGCGCACAGGCAACAAGGGCCTTCGGCTAAAAGAGAGTGGAGCCATCAACACCTCC
CTGTTTGTCCTGGGCAAAGTGGTAGATGCGCTGAATCAGGGCCTCCCTCGTGTACCTTAT
CGGGACAGCAAGCTCACTCGCCTATTGCAGGACTCTCTGGGTGGCTCAGCCCACAGTATC
CTTATTGCCAACATTGCCCCTGAGAGACGCTTCTACCTAGACACAGTCTCCGCACTCAAC
TTTGCTGCCAGGTCCAAGGAGGTGATCAATCGGCCTTTTACCAATGAGAGCCTGCAGCCT
CATGCCTTGGGACCTGTTAAGCTGTCTCAGAAAGAATTGCTTGGTCCACCAGAGGCAAAG
AGAGCCCGAGGCCCTGAGGAAGAGGAGATTGGGAGCCCTGAGCCCATGGCAGCTCCAGCC
TCTGCCTCCCAGAAACTCAGCCCCCTACAGAAGCTAAGCAGCATGGACCCGGCCATGCTG
GAGCGCCTCCTCAGCTTGGACCGTCTGCTTGCCTCCCAGGGGAGCCAGGGGGCCCCTCTG
TTGAGTACCCCAAAGCGAGAGCGGATGGTGCTAATGAAGACAGTAGAAGAGAAGGACCTA
GAGATTGAGAGGCTTAAGACGAAGCAAAAAGAACTGGAGGCCAAGATGTTGGCCCAGAAG
GCTGAGGAAAAGGAGAACCATTGTCCCACAATGCTCCGGCCCCTTTCACATCGCACAGTC
ACAGGGGCAAAGCCCCTGAAAAAGGCTGTGGTGATGCCCCTACAGCTAATTCAGGAGCAG
GCAGCATCCCCAAATGCCGAGATCCACATCCTGAAGAATAAAGGCCGGAAGAGAAAGCTG
GAGTCCCTGGATGCCCTAGAGCCTGAGGAGAAGGCTGAGGACTGCTGGGAGCTACAGATC
AGCCCGGAGCTACTGGCTCATGGGCGCCAAAAAATACTGGATCTGCTGAACGAAGGCTCA
GCCCGAGATCTCCGCAGTCTTCAGCGCATTGGCCCGAAGAAGGCCCAGCTAATCGTGGGC
TGGCGGGAGCTCCACGGCCCCTTCAGCCAGGTGGAGGACCTGGAACGCGTGGAGGGCATA
ACGGGGAAACAGATGGAGTCCTTCCTGAAGGCAAACATCCTGGGTCTCGCCGCCGGCCAG
CGCTGTGGCGCCTCC

ORF Protein

Cloned ORF protein sequence for pFN21AE4553 Download

>KIEE4553  665 aa
MAAGGSTQQRRREMAAASAAAISGAGRCRLSKIGATRRPPPARVRVAVRLRPFVDGTAGA
SDPPCVRGMDSCSLEIANWRNHQETLKYQFDAFYGERSTQQDIYAGSVQPILRHLLEGQN
ASVLAYGPTGAGKTHTMLGSPEQPGVIPRALMDLLQLTREEGAEGRPWALSVTMSYLEIY
QEKVLDLLDPASGDLVIREDCRGNILIPGLSQKPISSFADFERHFLPASRNRTVGATRLN
QRSSRSHAVLLVKVDQRERLAPFRQREGKLYLIDLAGSEDNRRTGNKGLRLKESGAINTS
LFVLGKVVDALNQGLPRVPYRDSKLTRLLQDSLGGSAHSILIANIAPERRFYLDTVSALN
FAARSKEVINRPFTNESLQPHALGPVKLSQKELLGPPEAKRARGPEEEEIGSPEPMAAPA
SASQKLSPLQKLSSMDPAMLERLLSLDRLLASQGSQGAPLLSTPKRERMVLMKTVEEKDL
EIERLKTKQKELEAKMLAQKAEEKENHCPTMLRPLSHRTVTGAKPLKKAVVMPLQLIQEQ
AASPNAEIHILKNKGRKRKLESLDALEPEEKAEDCWELQISPELLAHGRQKILDLLNEGS
ARDLRSLQRIGPKKAQLIVGWRELHGPFSQVEDLERVEGITGKQMESFLKANILGLAAGQ
RCGAS

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE4553 Download

>pFN21AE4553  6700 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCCGCGGGCGGCTCGACGCAGCAGAGGCGACG
CGAGATGGCGGCAGCTTCAGCGGCGGCGATCTCAGGAGCTGGTCGCTGTCGGCTAAGCAA
GATTGGAGCTACTCGTCGTCCACCTCCAGCTCGCGTAAGGGTGGCTGTGCGACTGCGGCC
ATTTGTGGATGGAACAGCGGGAGCAAGTGATCCCCCCTGTGTGCGGGGCATGGACAGCTG
CTCTCTAGAGATTGCTAACTGGAGGAACCACCAGGAGACTCTCAAATACCAGTTTGATGC
CTTCTATGGGGAGAGGAGTACTCAGCAGGACATCTATGCAGGTTCAGTGCAGCCCATCCT
AAGGCACTTGCTGGAAGGGCAGAATGCCAGTGTGCTTGCCTATGGACCCACAGGAGCTGG
GAAGACGCACACAATGCTGGGCAGCCCAGAGCAACCTGGGGTGATCCCGCGGGCTCTCAT
GGACCTCCTGCAGCTCACAAGGGAGGAGGGTGCCGAGGGCCGGCCATGGGCCCTTTCTGT
CACCATGTCTTACCTAGAGATCTACCAGGAGAAGGTATTAGACCTCCTGGACCCTGCTTC
GGGAGACCTGGTAATCCGAGAAGACTGCCGGGGGAATATCCTGATTCCGGGTCTCTCCCA
GAAGCCCATCAGTAGCTTTGCTGATTTTGAGCGGCACTTCCTGCCAGCCAGTCGAAATCG
GACTGTAGGAGCCACCCGGCTCAACCAGCGCTCCTCCCGCAGTCATGCTGTGCTCCTGGT
CAAGGTGGACCAGCGGGAACGTTTGGCCCCATTTCGCCAGCGAGAGGGAAAACTCTACCT
GATTGACTTGGCTGGGTCAGAGGACAACCGGCGCACAGGCAACAAGGGCCTTCGGCTAAA
AGAGAGTGGAGCCATCAACACCTCCCTGTTTGTCCTGGGCAAAGTGGTAGATGCGCTGAA
TCAGGGCCTCCCTCGTGTACCTTATCGGGACAGCAAGCTCACTCGCCTATTGCAGGACTC
TCTGGGTGGCTCAGCCCACAGTATCCTTATTGCCAACATTGCCCCTGAGAGACGCTTCTA
CCTAGACACAGTCTCCGCACTCAACTTTGCTGCCAGGTCCAAGGAGGTGATCAATCGGCC
TTTTACCAATGAGAGCCTGCAGCCTCATGCCTTGGGACCTGTTAAGCTGTCTCAGAAAGA
ATTGCTTGGTCCACCAGAGGCAAAGAGAGCCCGAGGCCCTGAGGAAGAGGAGATTGGGAG
CCCTGAGCCCATGGCAGCTCCAGCCTCTGCCTCCCAGAAACTCAGCCCCCTACAGAAGCT
AAGCAGCATGGACCCGGCCATGCTGGAGCGCCTCCTCAGCTTGGACCGTCTGCTTGCCTC
CCAGGGGAGCCAGGGGGCCCCTCTGTTGAGTACCCCAAAGCGAGAGCGGATGGTGCTAAT
GAAGACAGTAGAAGAGAAGGACCTAGAGATTGAGAGGCTTAAGACGAAGCAAAAAGAACT
GGAGGCCAAGATGTTGGCCCAGAAGGCTGAGGAAAAGGAGAACCATTGTCCCACAATGCT
CCGGCCCCTTTCACATCGCACAGTCACAGGGGCAAAGCCCCTGAAAAAGGCTGTGGTGAT
GCCCCTACAGCTAATTCAGGAGCAGGCAGCATCCCCAAATGCCGAGATCCACATCCTGAA
GAATAAAGGCCGGAAGAGAAAGCTGGAGTCCCTGGATGCCCTAGAGCCTGAGGAGAAGGC
TGAGGACTGCTGGGAGCTACAGATCAGCCCGGAGCTACTGGCTCATGGGCGCCAAAAAAT
ACTGGATCTGCTGAACGAAGGCTCAGCCCGAGATCTCCGCAGTCTTCAGCGCATTGGCCC
GAAGAAGGCCCAGCTAATCGTGGGCTGGCGGGAGCTCCACGGCCCCTTCAGCCAGGTGGA
GGACCTGGAACGCGTGGAGGGCATAACGGGGAAACAGATGGAGTCCTTCCTGAAGGCAAA
CATCCTGGGTCTCGCCGCCGGCCAGCGCTGTGGCGCCTCCGTTTAAACGAATTCGGGCTC
GGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACA
AAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCC
TTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACA
ACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTT
GTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTT
CAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGT
AAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAG
TAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTT
TCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTT
TTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAA
TAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTT
TTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGAT
GCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAG
ATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTG
CTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATA
CACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGAT
GGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCC
AACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATG
GGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAAC
GACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACT
GGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAA
GTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCT
GGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCC
TCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGA
CAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAA
GCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAG
GGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAA
AGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATC
GACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCC
CTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCG
CCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTT
CGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACC
GCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGC
CACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAG
AGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCG
CTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAA
CCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAG
GATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACT
CACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAT
AGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAA
AAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAG
GTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTG
CCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATT
AAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGT
ACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT