################################## # NO CUT ENZYMES ( 104 enzymes) # ################################## AatII Acc65I AccI AccIII AcsI AgeI AhdI Alw44I ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AviII AvrII BalI BamHI BbrPI BlnI BsaAI BsaWI BseAI BsiEI BsiWI Bsp1407I BspDI BspEI BspHI BspLU11I BsrGI BssHII Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco52I EcoO65I EcoRI EcoRV EcoT22I FspI HincII HindII HindIII HpaI KpnI MfeI MluNI MroI MscI MslI MunI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshAI PshBI PvuI RcaI RsrII SalI SapI SbfI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Van91I XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 30 enzymes) # ################################### AclI 773 AACGTT AA^CGTT AflII 1453 CTTAAG C^TTAAG AflIII 1905 ACGCGT A^C[GA][CT]GT AspI 1060 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC BcgI 592 CGAGAAGACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 1016 GTATCC GTATCC(6/5) BclI 1103 TGATCA T^GATCA BfrI 1453 CTTAAG C^TTAAG BglI 1492 GCCCAGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaBI 1641 GATCCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsgI 322 GTGCAG GTGCAG(16/14) BsmBI 1044 GAGACG CGTCTC(1/5) BsmI 357 GAATGC GAATGC(1/-1) BspMI 313 GCAGGT ACCTGC(4/8) BsrDI 1032 CATTGC GCAATG(2/0) BssSI 947 CTCGTG CACGAG(-5/-1) FbaI 1103 TGATCA T^GATCA FseI 493 GGCCGGCC GGCCGG^CC MaeII 774 ACGT A^CGT MamI 1641 GATCCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluI 1905 ACGCGT A^CGCGT PflFI 1060 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC Psp1406I 773 AACGTT AA^CGTT PvuII 210 CAGCTG CAG^CTG SacI 1867 GAGCTC GAGCT^C ScaI 292 AGTACT AGT^ACT SexAI 581 ACCTGGT A^CC[AT]GGT SmlI 1453 CTTAAG C^T[CT][GA]AG Tth111I 1060 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 219 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AcyI 1763 GGCGCC G[GA]^CG[CT]C 1987 GGCGCC Aor51HI 722 AGCGCT AGC^GCT 1979 AGCGCT ApaI 503 GGGCCC GGGCC^C 1370 GGGCCC AspHI 745 GTGCTC G[AT]GC[AT]^C 1867 GAGCTC AvaI 1205 CCCGAG C^[CT]CG[GA]G 1802 CCCGAG BbeI 1763 GGCGCC GGCGC^C 1987 GGCGCC BglII 533 AGATCT A^GATCT 1806 AGATCT BsaHI 1763 GGCGCC G[GA]^CG[CT]C 1987 GGCGCC BsiHKAI 745 GTGCTC G[AT]GC[AT]^C 1867 GAGCTC BsoBI 1205 CCCGAG C^[CT]CG[GA]G 1802 CCCGAG BsrBI 664 GAGCGG CCGCTC(-3/-3) 1399 GAGCGG BstAPI 402 GCACACAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 669 GCACTTCCTGC BstXI 1481 CCAAGATGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1977 CCAGCGCTGTGG Eco47III 722 AGCGCT AGC^GCT 1979 AGCGCT Hin1I 1763 GGCGCC G[GA]^CG[CT]C 1987 GGCGCC KasI 1763 GGCGCC G^GCGCC 1987 GGCGCC KspI 5 CCGCGG CCGC^GG 442 CCGCGG MaeIII 514 GTCAC ^GT[ACGT]AC 1558 GTCAC MseI 1157 TTAA T^TAA 1454 TTAA NaeI 494 GCCGGC GCC^GGC 1972 GCCGGC NarI 1763 GGCGCC GG^CGCC 1987 GGCGCC NcoI 499 CCATGG C^CATGG 1244 CCATGG NgoMIV 494 GCCGGC G^CCGGC 1972 GCCGGC PstI 463 CTGCAG CTGCA^G 1132 CTGCAG SacII 5 CCGCGG CCGC^GG 442 CCGCGG SfoI 1763 GGCGCC GGC^GCC 1987 GGCGCC TaqI 16 TCGA T^CGA 687 TCGA TfiI 618 GATTC G^A[AT]TC 933 GAATC Tru9I 1157 TTAA T^TAA 1454 TTAA Tsp45I 514 GTCAC ^GT[GC]AC 1558 GTCAC Tsp509I 1175 AATT ^AATT 1608 AATT TspRI 320 CAGTG CA[GC]TG[ACGT][ACGT]^ 363 CAGTG TthHB8I 16 TCGA T^CGA 687 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 91 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AlwNI AocI AvaII BanI BanII BbsI BbvCI BbvI BcnI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaI BsaJI BseRI BsiYI BslI BsmAI BsmFI Bsp1286I BsrFI BsrI BssKI BstF5I BstNI BstUI BstYI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MboI MboII MflI MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TseI XhoII