GNA14 ORF clone FHC09220

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC09220

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Clone info.

Species

human

Clone Name

pFN21AE3666 Sequence (with vector) Restriction Map

Product ID

FHC09220

ORF Sequence

DNA sequence (1065 bp)
Protein sequence (355 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE3666

Symbol

GNA14

Description

guanine nucleotide binding protein (G protein), alpha 14

Original Clone ID

oc93666

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1065 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 355 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS6657.1 1.7e-143 100.0 GNA14 CCDS6658.1 1.5e-119 81.8 GNAQ CCDS12103.1 3.6e-119 82.3 GNA11 CCDS12104.1 3.1e-70 54.1 GNA15 CCDS10757.1 1.7e-69 52.9 GNAO1 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR000654 5 19 PR00442 G-protein alpha subunit IPR001019 37 52 PR00318 Guanine nucleotide binding protein (G-protein) IPR000654 77 87 PR00442 G-protein alpha subunit IPR001019 168 190 PR00318 Guanine nucleotide binding protein (G-protein) IPR001019 197 214 PR00318 Guanine nucleotide binding protein (G-protein) IPR001019 219 247 PR00318 Guanine nucleotide binding protein (G-protein) IPR000654 255 265 PR00442 G-protein alpha subunit IPR001019 265 274 PR00318 Guanine nucleotide binding protein (G-protein) IPR000654 293 303 PR00442 G-protein alpha subunit IPR000654 307 318 PR00442 G-protein alpha subunit HMMPfam IPR001019 9 354 PF00503 Guanine nucleotide binding protein (G-protein) HMMSmart IPR001019 15 354 SM00275 Guanine nucleotide binding protein (G-protein)
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_004288 355 604397 100.0 100.0 XP_016870830 301 604397 100.0 84.8 NP_002063 359 163000,185300,600998 81.8 98.9 NP_002058 359 139313 82.3 98.9 XP_016870117 301 163000,185300,600998 81.1 84.8 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE3666 Download

>KIEE3666  1065 bp
ATGGCCGGCTGCTGCTGCCTGTCCGCGGAGGAGAAGGAGTCGCAGCGCATCAGCGCGGAG
ATCGAGCGACAGCTTCGTCGGGACAAGAAGGACGCGCGCCGTGAGCTTAAGCTGCTGCTG
CTGGGAACTGGTGAAAGTGGGAAAAGCACCTTTATCAAGCAGATGAGAATTATCCATGGG
TCTGGTTACAGCGACGAAGACAGAAAGGGGTTCACGAAGCTGGTTTACCAAAACATATTC
ACCGCCATGCAAGCCATGATCAGAGCGATGGACACGCTAAGGATACAGTATGTGTGTGAA
CAGAATAAGGAAAATGCCCAGATAATCAGAGAAGTGGAAGTGGACAAGGTCTCCATGCTC
TCCAGGGAGCAGGTGGAGGCCATCAAGCAGCTCTGGCAAGATCCAGGCATCCAGGAGTGT
TACGACAGGAGGAGGGAGTACCAGCTGTCGGACTCTGCCAAATATTACCTGACTGACATT
GACCGCATCGCCACACCATCATTCGTGCCTACCCAACAAGATGTGCTTCGCGTCCGAGTG
CCCACCACCGGCATCATTGAGTATCCATTTGACTTGGAAAACATCATCTTTCGGATGGTG
GATGTTGGTGGCCAACGATCGGAAAGACGGAAGTGGATTCACTGCTTTGAGAGTGTCACC
TCCATTATTTTCTTGGTTGCTCTGAGTGAATATGACCAGGTCCTGGCTGAGTGTGACAAC
GAGAATCGCATGGAAGAGAGCAAAGCCTTATTTAAAACCATCATCACCTACCCCTGGTTT
CTGAATTCGTCTGTGATTTTATTCTTGAACAAGAAGGATCTTTTGGAAGAGAAAATCATG
TACTCTCATCTAATTAGCTATTTCCCAGAATACACAGGACCGAAACAGGATGTCAGAGCT
GCCAGAGACTTTATCCTGAAGCTTTACCAAGATCAGAATCCTGACAAAGAGAAAGTCATC
TACTCTCACTTCACATGTGCTACAGATACAGACAATATTCGCTTTGTGTTTGCTGCTGTC
AAAGACACAATTCTACAGCTAAACCTAAGGGAATTCAACCTTGTC

ORF Protein

Cloned ORF protein sequence for pFN21AE3666 Download

>KIEE3666  355 aa
MAGCCCLSAEEKESQRISAEIERQLRRDKKDARRELKLLLLGTGESGKSTFIKQMRIIHG
SGYSDEDRKGFTKLVYQNIFTAMQAMIRAMDTLRIQYVCEQNKENAQIIREVEVDKVSML
SREQVEAIKQLWQDPGIQECYDRRREYQLSDSAKYYLTDIDRIATPSFVPTQQDVLRVRV
PTTGIIEYPFDLENIIFRMVDVGGQRSERRKWIHCFESVTSIIFLVALSEYDQVLAECDN
ENRMEESKALFKTIITYPWFLNSSVILFLNKKDLLEEKIMYSHLISYFPEYTGPKQDVRA
ARDFILKLYQDQNPDKEKVIYSHFTCATDTDNIRFVFAAVKDTILQLNLREFNLV

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE3666 Download

>pFN21AE3666  5770 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCCGGCTGCTGCTGCCTGTCCGCGGAGGAGAA
GGAGTCGCAGCGCATCAGCGCGGAGATCGAGCGACAGCTTCGTCGGGACAAGAAGGACGC
GCGCCGTGAGCTTAAGCTGCTGCTGCTGGGAACTGGTGAAAGTGGGAAAAGCACCTTTAT
CAAGCAGATGAGAATTATCCATGGGTCTGGTTACAGCGACGAAGACAGAAAGGGGTTCAC
GAAGCTGGTTTACCAAAACATATTCACCGCCATGCAAGCCATGATCAGAGCGATGGACAC
GCTAAGGATACAGTATGTGTGTGAACAGAATAAGGAAAATGCCCAGATAATCAGAGAAGT
GGAAGTGGACAAGGTCTCCATGCTCTCCAGGGAGCAGGTGGAGGCCATCAAGCAGCTCTG
GCAAGATCCAGGCATCCAGGAGTGTTACGACAGGAGGAGGGAGTACCAGCTGTCGGACTC
TGCCAAATATTACCTGACTGACATTGACCGCATCGCCACACCATCATTCGTGCCTACCCA
ACAAGATGTGCTTCGCGTCCGAGTGCCCACCACCGGCATCATTGAGTATCCATTTGACTT
GGAAAACATCATCTTTCGGATGGTGGATGTTGGTGGCCAACGATCGGAAAGACGGAAGTG
GATTCACTGCTTTGAGAGTGTCACCTCCATTATTTTCTTGGTTGCTCTGAGTGAATATGA
CCAGGTCCTGGCTGAGTGTGACAACGAGAATCGCATGGAAGAGAGCAAAGCCTTATTTAA
AACCATCATCACCTACCCCTGGTTTCTGAATTCGTCTGTGATTTTATTCTTGAACAAGAA
GGATCTTTTGGAAGAGAAAATCATGTACTCTCATCTAATTAGCTATTTCCCAGAATACAC
AGGACCGAAACAGGATGTCAGAGCTGCCAGAGACTTTATCCTGAAGCTTTACCAAGATCA
GAATCCTGACAAAGAGAAAGTCATCTACTCTCACTTCACATGTGCTACAGATACAGACAA
TATTCGCTTTGTGTTTGCTGCTGTCAAAGACACAATTCTACAGCTAAACCTAAGGGAATT
CAACCTTGTCGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTG
CAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTG
CCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATA
AGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATT
TGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTT
AACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTT
TTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCG
CTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGT
TTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGA
AATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTC
ATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATT
CAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCT
CACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGT
TACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGT
TTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGAC
GCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTAC
TCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCT
GCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCG
AAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGG
GAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCA
ATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAA
CAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTT
CCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATC
ATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGG
AGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATT
AAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAA
AGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAA
AAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGC
TCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGA
CAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTC
CGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTT
CTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCT
GTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTG
AGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTA
GCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCT
ACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAA
GAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTT
GCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTA
CGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTAT
CAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGAT
GGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGG
TAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGC
GATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGC
GCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCT
GCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGG
ATCGAGATCT