################################## # NO CUT ENZYMES ( 132 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AvaI AviII AvrII BamHI BanI BanII BbeI BbrPI BbvCI BcgI BcnI BfaI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaHI BsaWI BseAI BsgI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BsrBI BsrDI BsrGI BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I CelII ClaI CpoI DraIII EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRV EcoT22I FauI FseI FspI HaeII Hin1I HincII HindII HpaI KasI KpnI MaeI MaeII MfeI MluI MroI MunI NarI NciI NdeI NheI NlaIV NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI RcaI RsrII SacI SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AflII 106 CTTAAG C^TTAAG AflIII 973 ACATGT A^C[GA][CT]GT AlwNI 697 CAGGTCCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 1044 CCTAAGG CC^T[ACGT]AGG BalI 609 TGGCCA TGG^CCA BbsI 196 GAAGAC GAAGAC(2/6) BclI 257 TGATCA T^GATCA BfrI 106 CTTAAG C^TTAAG BmyI 538 GTGCCC G[AGT]GC[ACT]^C BpmI 360 CTCCAG CTGGAG(16/14) BpuAI 196 GAAGAC GAAGAC(2/6) BsaBI 931 GATCAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 348 GGTCTC GGTCTC(1/5) BsiEI 616 CGATCG CG[GA][CT]^CG BsmFI 80 GGGAC GGGAC(10/14) Bsp1286I 538 GTGCCC G[AGT]GC[ACT]^C BspLU11I 973 ACATGT A^CATGT BspMI 369 GCAGGT ACCTGC(4/8) BsrI 127 ACTGG ACTGG(1/-1) BssHII 94 GCGCGC G^CGCGC BstXI 174 CCATGGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 1044 CCTAAGG CC^T[ACGT]AGG DraI 751 TTTAAA TTT^AAA DraII 698 AGGTCCT [GA]G^G[ACGT]CC[CT] DrdI 68 GACAGCTTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 1044 CCTAAGG CC^T[ACGT]AGG EcoO109I 698 AGGTCCT [GA]G^G[ACGT]CC[CT] EcoT14I 174 CCATGG C^C[AT][AT]GG FbaI 257 TGATCA T^GATCA HindIII 919 AAGCTT A^AGCTT KspI 23 CCGCGG CCGC^GG MamI 931 GATCAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 609 TGGCCA TGG^CCA MscI 609 TGGCCA TGG^CCA MslI 240 CACCGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NaeI 4 GCCGGC GCC^GGC NcoI 174 CCATGG C^CATGG NgoMIV 4 GCCGGC G^CCGGC NspI 973 ACATGT [GA]CATG^[CT] PflMI 696 CCAGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 698 AGGTCCT [GA]G^G[AT]CC[CT] PvuI 616 CGATCG CGAT^CG PvuII 442 CAGCTG CAG^CTG SacII 23 CCGCGG CCGC^GG SexAI 695 ACCAGGT A^CC[AT]GGT SmlI 106 CTTAAG C^T[CT][GA]AG StyI 174 CCATGG C^C[AT][AT]GG TaqI 62 TCGA T^CGA TspRI 640 CACTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 62 TCGA T^CGA Van91I 696 CCAGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 36 enzymes) # ################################### AcsI 783 GAATTC [GA]^AATT[CT] 1051 GAATTC AfaI 438 GTAC GT^AC 840 GTAC AlwI 400 GATCC GGATC(4/5) 816 GGATC ApoI 783 GAATTC [GA]^AATT[CT] 1051 GAATTC AspI 343 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC 694 GACCAGGTC AvaII 699 GGTCC G^G[AT]CC 877 GGACC BciVI 281 GGATAC GTATCC(6/5) 561 GTATCC BseRI 28 GAGGAG GAGGAG(10/8) 429 GAGGAG BsmAI 905 GAGAC GTCTC(1/5) 349 GTCTC BsrFI 4 GCCGGC [GA]^CCGG[CT] 547 ACCGGC BstYI 399 AGATCC [GA]^GATC[CT] 816 GGATCT Cfr10I 4 GCCGGC [GA]^CCGG[CT] 547 ACCGGC Cfr13I 699 GGTCC G^G[ACGT]CC 877 GGACC DsaI 23 CCGCGG C^C[GA][CT]GG 174 CCATGG EaeI 2 TGGCCG [CT]^GGCC[GA] 609 TGGCCA EarI 733 GAAGAG CTCTTC(1/4) 826 GAAGAG EcoRI 783 GAATTC G^AATTC 1051 GAATTC HapII 5 CCGG C^CGG 548 CCGG HgaI 91 GACGC GACGC(5/10) 530 GCGTC HpaII 5 CCGG C^CGG 548 CCGG Ksp632I 733 GAAGAG CTCTTC(1/4) 826 GAAGAG MflI 399 AGATCC [GA]^GATC[CT] 816 GGATCT MseI 107 TTAA T^TAA 752 TTAA MspA1I 23 CCGCGG C[AC]G^C[GT]G 442 CAGCTG MspI 5 CCGG C^CGG 548 CCGG PflFI 343 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC 694 GACCAGGTC PleI 451 GACTC GAGTC(4/5) 37 GAGTC RsaI 438 GTAC GT^AC 840 GTAC Sau96I 699 GGTCC G^G[ACGT]CC 877 GGACC SfcI 980 CTACAG C^T[GA][CT]AG 1033 CTACAG SspI 461 AATATT AAT^ATT 994 AATATT Tru9I 107 TTAA T^TAA 752 TTAA Tsp45I 655 GTCAC ^GT[GC]AC 713 GTGAC Tth111I 343 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC 694 GACCAGGTC XcmI 362 CCAGGGAGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 662 CCATTATTTTCTTGG XhoII 399 AGATCC [GA]^GATC[CT] 816 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 39 enzymes) # ######################################### AccII AciI AluI BbvI BsaJI BsiYI BslI BssKI BstF5I BstNI BstUI Cac8I CfoI DdeI DpnII EcoRII Fnu4HI FokI HaeIII HhaI HinP1I HinfI HphI ItaI MaeIII MboI MboII MnlI MvaI MvnI MwoI NdeII NlaIII Sau3AI ScrFI SfaNI TfiI TseI Tsp509I