HEMK1 ORF clone FHC07917

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC07917

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Clone info.

Species

human

Clone Name

pFN21AE2223 Sequence (with vector) Restriction Map

Product ID

FHC07917

ORF Sequence

DNA sequence (1014 bp)
Protein sequence (338 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB590120]

Gene

KIEE2223

Symbol

HEMK1

Alias : HEMK, MPRMC, MTQ1

Description

HemK methyltransferase family member 1

Original Clone ID

cs03323

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1014 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 338 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS2830.1 6.2e-150 100.0 HEMK1 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR002295 233 245 PR00506 N6 adenine-specific DNA methyltransferase IPR002295 277 296 PR00506 N6 adenine-specific DNA methyltransferase HMMPfam IPR007848 163 293 PF05175 Methyltransferase small HMMTigr IPR004556 61 303 TIGR00536 Modification methylase HemK IPR019874 65 331 TIGR03534 Protein-(glutamine-N5) methyltransferase Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE2223 Download

>KIEE2223  1014 bp
ATGGAGCTTTGGGGCCGAATGCTGTGGGCCCTCCTGTCTGGCCCAGGGAGGAGGGGAAGT
ACCCGGGGCTGGGCCTTCAGCTCATGGCAACCCCAACCACCTCTGGCTGGGTTATCCAGT
GCCATAGAACTGGTCAGCCACTGGACTGGGGTCTTTGAGAAGAGGGGTATCCCTGAGGCC
CGGGAATCCAGTGAGTACATCGTGGCTCATGTCCTTGGAGCCAAAACATTTCAGAGCCTG
AGGCCGGCACTTTGGACCCAGCCCTTGACCTCTCAGCAACTACAGTGTATCCGGGAGCTG
AGTAGCCGTCGATTGCAGAGGATGCCGGTGCAGTACATCCTTGGAGAGTGGGACTTCCAG
GGGCTCAGCCTAAGGATGGTGCCCCCAGTGTTTATTCCGCGGCCAGAAACAGAGGAACTG
GTTGAGTGGGTGCTGGAAGAGGTGGCCCAGAGGTCCCATGCTGTGGGATCCCCAGGCAGC
CCCCTCATTCTGGAGGTGGGCTGCGGATCAGGAGCCATCTCCCTCAGCCTGCTGAGCCAG
CTCCCCCAGAGCCGAGTCATTGCTGTGGATAAGCGGGAAGCTGCTATCTCTCTGACCCAT
GAGAATGCTCAGAGGCTTCGGTTGCAGGACAGGATTTGGATCATCCACCTCGACATGACC
TCAGAAAGGAGCTGGACACACCTGCCCTGGGGCCCCATGGACCTGATTGTCAGCAACCCT
CCCTACGTCTTCCACCAGGACATGGAGCAGCTGGCCCCTGAGATCCGCAGCTATGAAGAC
CCCGCGGCCCTGGATGGTGGGGAGGAGGGCATGGACATCATTACCCACATTCTGGCCTTG
GCACCCCGGCTCCTGAAAGACTCTGGTAGTATCTTCTTAGAAGTGGACCCAAGGCACCCG
GAGCTTGTCAGCAGCTGGCTTCAGAGCCGGCCTGACCTGTACCTTAATCTTGTGGCTGTG
CGCAGGGACTTCTGTGGGAGGCCCCGGTTCCTGCATATCCGGAGGTCTGGGCCA

ORF Protein

Cloned ORF protein sequence for pFN21AE2223 Download

>KIEE2223  338 aa
MELWGRMLWALLSGPGRRGSTRGWAFSSWQPQPPLAGLSSAIELVSHWTGVFEKRGIPEA
RESSEYIVAHVLGAKTFQSLRPALWTQPLTSQQLQCIRELSSRRLQRMPVQYILGEWDFQ
GLSLRMVPPVFIPRPETEELVEWVLEEVAQRSHAVGSPGSPLILEVGCGSGAISLSLLSQ
LPQSRVIAVDKREAAISLTHENAQRLRLQDRIWIIHLDMTSERSWTHLPWGPMDLIVSNP
PYVFHQDMEQLAPEIRSYEDPAALDGGEEGMDIITHILALAPRLLKDSGSIFLEVDPRHP
ELVSSWLQSRPDLYLNLVAVRRDFCGRPRFLHIRRSGP

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE2223 Download

>pFN21AE2223  5719 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGAGCTTTGGGGCCGAATGCTGTGGGCCCTCCT
GTCTGGCCCAGGGAGGAGGGGAAGTACCCGGGGCTGGGCCTTCAGCTCATGGCAACCCCA
ACCACCTCTGGCTGGGTTATCCAGTGCCATAGAACTGGTCAGCCACTGGACTGGGGTCTT
TGAGAAGAGGGGTATCCCTGAGGCCCGGGAATCCAGTGAGTACATCGTGGCTCATGTCCT
TGGAGCCAAAACATTTCAGAGCCTGAGGCCGGCACTTTGGACCCAGCCCTTGACCTCTCA
GCAACTACAGTGTATCCGGGAGCTGAGTAGCCGTCGATTGCAGAGGATGCCGGTGCAGTA
CATCCTTGGAGAGTGGGACTTCCAGGGGCTCAGCCTAAGGATGGTGCCCCCAGTGTTTAT
TCCGCGGCCAGAAACAGAGGAACTGGTTGAGTGGGTGCTGGAAGAGGTGGCCCAGAGGTC
CCATGCTGTGGGATCCCCAGGCAGCCCCCTCATTCTGGAGGTGGGCTGCGGATCAGGAGC
CATCTCCCTCAGCCTGCTGAGCCAGCTCCCCCAGAGCCGAGTCATTGCTGTGGATAAGCG
GGAAGCTGCTATCTCTCTGACCCATGAGAATGCTCAGAGGCTTCGGTTGCAGGACAGGAT
TTGGATCATCCACCTCGACATGACCTCAGAAAGGAGCTGGACACACCTGCCCTGGGGCCC
CATGGACCTGATTGTCAGCAACCCTCCCTACGTCTTCCACCAGGACATGGAGCAGCTGGC
CCCTGAGATCCGCAGCTATGAAGACCCCGCGGCCCTGGATGGTGGGGAGGAGGGCATGGA
CATCATTACCCACATTCTGGCCTTGGCACCCCGGCTCCTGAAAGACTCTGGTAGTATCTT
CTTAGAAGTGGACCCAAGGCACCCGGAGCTTGTCAGCAGCTGGCTTCAGAGCCGGCCTGA
CCTGTACCTTAATCTTGTGGCTGTGCGCAGGGACTTCTGTGGGAGGCCCCGGTTCCTGCA
TATCCGGAGGTCTGGGCCAGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGA
GTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGT
TGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCA
GACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAA
TGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAAT
AAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGG
GAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATC
CTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGT
CAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACT
TTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATG
TATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT
ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCT
GTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCA
CGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCC
GAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCC
CGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTG
GTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTA
TGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATC
GGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTT
GATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATG
CCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCT
TCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGC
TCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCT
CGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTAC
ACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCC
TCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAG
CTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACA
TGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTT
TCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGC
GAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCT
CTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCG
TGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCA
AGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACT
ATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTA
ACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTA
ACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCT
TCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTT
TTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGA
TCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCA
TGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGC
ACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATT
AAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTT
ATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGC
CGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCAC
TTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCG
GCGTAGAGGATCGAGATCT