(Linear) (Six-base) MAPSORT of: KIEE2223.seq Check: 8822 from: 1 to: 1014 KIEE2223.orf With 182 enzymes: SgfI * July 15, 2010 18:56 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 689 1014 Size: 689 325 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 60 904 1014 Size: 60 844 110 Fragments arranged by size: 844 110 60 AlwNI CAG_nnn'CTG Cuts at: 0 140 237 914 1014 Size: 140 97 677 100 Fragments arranged by size: 677 140 100 97 ApaI G_GGCC'C Cuts at: 0 30 694 1014 Size: 30 664 320 Fragments arranged by size: 664 320 30 AvaI C'yCGr_G Cuts at: 0 62 179 1014 Size: 62 117 835 Fragments arranged by size: 835 117 62 BamHI G'GATC_C Cuts at: 0 466 1014 Size: 466 548 BanI G'GyrC_C Cuts at: 0 378 840 893 1014 Size: 378 462 53 121 Fragments arranged by size: 462 378 121 53 BanII G_rGCy'C Cuts at: 0 30 365 694 1014 Size: 30 335 329 320 Fragments arranged by size: 335 329 320 30 BbsI GAAGACnn'nnnn_ Cuts at: 0 720 782 1014 Size: 720 62 232 Fragments arranged by size: 720 232 62 BbvCI CC'TCA_GC Cuts at: 0 523 1014 Size: 523 491 BciVI GTATCCnnnnn_n' Cuts at: 0 178 298 1014 Size: 178 120 716 Fragments arranged by size: 716 178 120 BlpI GC'TnA_GC Cuts at: 0 364 532 1014 Size: 364 168 482 Fragments arranged by size: 482 364 168 Bme1580I G_kGCm'C Cuts at: 0 30 383 694 1014 Size: 30 353 311 320 Fragments arranged by size: 353 320 311 30 BmrI ACTGGGnnnn_n' Cuts at: 0 155 379 1014 Size: 155 224 635 Fragments arranged by size: 635 224 155 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 511 1014 Size: 511 503 Bpu10I CC'TnA_GC Cuts at: 0 523 1014 Size: 523 491 BsaBI GATnn'nnATC Cuts at: 0 637 1014 Size: 637 377 BsaWI w'CCGG_w Cuts at: 0 998 1014 Size: 998 16 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 42 72 833 863 1014 Size: 42 30 761 30 151 Fragments arranged by size: 761 151 42 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 63 817 1014 Size: 63 754 197 Fragments arranged by size: 754 197 63 BseYI C'CCAG_C Cuts at: 0 68 106 257 1014 Size: 68 38 151 757 Fragments arranged by size: 757 151 68 38 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 349 1014 Size: 349 665 BsmI GAATG_Cn' Cuts at: 0 23 609 1014 Size: 23 586 405 Fragments arranged by size: 586 405 23 Bsp1286I G_dGCh'C Cuts at: 0 30 365 383 694 1014 Size: 30 335 18 311 320 Fragments arranged by size: 335 320 311 30 18 BspEI T'CCGG_A Cuts at: 0 998 1014 Size: 998 16 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 689 1014 Size: 689 325 BsrDI GCAATG_nn' Cuts at: 0 557 1014 Size: 557 457 BsrFI r'CCGG_y Cuts at: 0 243 324 926 1014 Size: 243 81 602 88 Fragments arranged by size: 602 243 88 81 BstAPI GCAn_nnn'nTGC Cuts at: 0 321 1014 Size: 321 693 BstXI CCAn_nnnn'nTGG Cuts at: 0 129 145 1014 Size: 129 16 869 Fragments arranged by size: 869 129 16 BstYI r'GATC_y Cuts at: 0 466 761 1014 Size: 466 295 253 Fragments arranged by size: 466 295 253 Bsu36I CC'TnA_GG Cuts at: 0 173 238 370 1014 Size: 173 65 132 644 Fragments arranged by size: 644 173 132 65 BtgI C'CryG_G Cuts at: 0 397 695 782 1014 Size: 397 298 87 232 Fragments arranged by size: 397 298 232 87 DrdI GACnn_nn'nnGTC Cuts at: 0 706 1014 Size: 706 308 EaeI y'GGCC_r Cuts at: 0 400 1014 Size: 400 614 EarI CTCTTCn'nnn_ Cuts at: 0 154 431 1014 Size: 154 277 583 Fragments arranged by size: 583 277 154 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 60 511 904 1014 Size: 60 451 393 110 Fragments arranged by size: 451 393 110 60 EcoNI CCTnn'n_nnAGG Cuts at: 0 663 1014 Size: 663 351 EcoO109I rG'GnC_Cy Cuts at: 0 27 452 690 691 980 1014 Size: 27 425 238 1 289 34 Fragments arranged by size: 425 289 238 34 27 1 FspI TGC'GCA Cuts at: 0 961 1014 Size: 961 53 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 291 323 643 675 1014 Size: 291 32 320 32 339 Fragments arranged by size: 339 320 291 32 32 MslI CAynn'nnrTG Cuts at: 0 562 602 1014 Size: 562 40 412 Fragments arranged by size: 562 412 40 MspA1I CmG'CkG Cuts at: 0 399 750 784 914 1014 Size: 399 351 34 130 100 Fragments arranged by size: 399 351 130 100 34 NaeI GCC'GGC Cuts at: 0 245 928 1014 Size: 245 683 86 Fragments arranged by size: 683 245 86 NcoI C'CATG_G Cuts at: 0 695 1014 Size: 695 319 NgoMIV G'CCGG_C Cuts at: 0 243 926 1014 Size: 243 683 88 Fragments arranged by size: 683 243 88 PasI CC'CwG_GG Cuts at: 0 43 686 1014 Size: 43 643 328 Fragments arranged by size: 643 328 43 PflMI CCAn_nnn'nTGG Cuts at: 0 462 741 831 1014 Size: 462 279 90 183 Fragments arranged by size: 462 279 183 90 PfoI T'CCnGG_A Cuts at: 0 290 1014 Size: 290 724 PpuMI rG'GwC_Cy Cuts at: 0 452 1014 Size: 452 562 PshAI GACnn'nnGTC Cuts at: 0 148 1014 Size: 148 866 PspOMI G'GGCC_C Cuts at: 0 26 690 1014 Size: 26 664 324 Fragments arranged by size: 664 324 26 PvuII CAG'CTG Cuts at: 0 750 914 1014 Size: 750 164 100 Fragments arranged by size: 750 164 100 SacII CC_GC'GG Cuts at: 0 400 785 1014 Size: 400 385 229 Fragments arranged by size: 400 385 229 SfcI C'TryA_G Cuts at: 0 280 1014 Size: 280 734 SmaI CCC'GGG Cuts at: 0 64 181 1014 Size: 64 117 833 Fragments arranged by size: 833 117 64 StyI C'CwwG_G Cuts at: 0 213 339 695 836 889 1014 Size: 213 126 356 141 53 125 Fragments arranged by size: 356 213 141 126 125 53 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 417 1014 Size: 417 597 TatI w'GTAC_w Cuts at: 0 194 332 1014 Size: 194 138 682 Fragments arranged by size: 682 194 138 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 833 865 1014 Size: 833 32 149 Fragments arranged by size: 833 149 32 XmaI C'CCGG_G Cuts at: 0 62 179 1014 Size: 62 117 835 Fragments arranged by size: 835 117 62 Enzymes that do cut: AarI AcuI AlwNI ApaI AvaI BamHI BanI BanII BbsI BbvCI BciVI BlpI Bme1580I BmrI BpmI Bpu10I BsaBI BsaWI BsaXI BseRI BseYI BsgI BsmI Bsp1286I BspEI BspMI BsrDI BsrFI BstAPI BstXI BstYI Bsu36I BtgI DrdI EaeI EarI Eco57MI EcoNI EcoO109I FspI Hin4I MslI MspA1I NaeI NcoI NgoMIV PasI PflMI PfoI PpuMI PshAI PspOMI PvuII SacII SfcI SmaI StyI TaqII TatI TstI XmaI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BbeI BcgI BclI BfrBI BglI BglII BmgBI BmtI BplI BpuEI BsaI BsaAI BsaHI BsiEI BsiHKAI BsiWI BsmBI BspHI BsrBI BsrGI BssHII BssSI BstBI BstEII BstZ17I BtgZI BtsI ClaI CspCI DraI DraIII EagI EciI EcoICRI EcoRI EcoRV FalI FseI FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI NarI NdeI NheI NotI NruI NsiI NspI PacI PciI PmeI PmlI PpiI PsiI PspXI PsrI PstI PvuI RsrII SacI SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I XbaI XcmI XhoI XmnI ZraI