DDX50 ORF clone FHC07845

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC07845

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Clone info.

Species

human

Clone Name

pFN21AE5708 Sequence (with vector) Restriction Map

Product ID

FHC07845

ORF Sequence

DNA sequence (2211 bp)
Protein sequence (737 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE5708

Symbol

DDX50

Alias : GU2, GUB, RH-II/GuB, mcdrh

Description

DEAD (Asp-Glu-Ala-Asp) box polypeptide 50

Original Clone ID

oc95708

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 2211 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 737 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS7283.1 2.9e-198 100.0 DDX50 CCDS31211.1 3.1e-107 60.8 DDX21 CCDS73144.1 3.2e-107 60.8 DDX21 CCDS33646.1 2.9e-25 34.4 DDX17 CCDS46706.1 2.9e-25 34.4 DDX17 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR011545 161 335 PF00270 DNA/RNA helicase IPR001650 414 483 PF00271 Helicase IPR012562 571 666 PF08152 GUCT HMMSmart IPR014001 156 357 SM00487 DEAD-like helicase IPR001650 401 483 SM00490 Helicase ProfileScan IPR014014 137 165 PS51195 RNA helicase IPR014001 168 347 PS51192 DEAD-like helicase IPR001650 380 524 PS51194 Helicase
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_076950 737 610373 100.0 100.0 XP_016872115 737 610373 100.0 100.0 XP_016872117 672 610373 100.0 91.2 XP_011538446 672 610373 100.0 91.2 XP_005270205 672 610373 100.0 91.2 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE5708 Download

>KIEE5708  2211 bp
ATGCCTGGGAAACTCCTCTGGGGGGACATTATGGAGCTGGAAGCACCCTTGGAGGAGTCC
GAGAGCCAGAAGAAGGAGAGGCAAAAGAGTGACAGAAGGAAGTCAAGGCACCATTATGAC
TCGGATGAGAAATCAGAAACAAGAGAAAATGGTGTTACAGATGACCTGGATGCTCCCAAG
GCCAAAAAATCTAAAATGAAAGAGAAGCTAAATGGAGACACTGAAGAAGGATTTAATAGA
CTTTCAGATGAATTCTCCAAATCTCATAAGTCAAGAAGAAAAGATCTACCAAATGGAGAT
ATAGATGAATATGAAAAAAAATCAAAGCGAGTATCATCTTTAGATACTTCTACTCATAAA
TCAAGTGATAATAAACTAGAGGAGACCTTAACACGTGAACAGAAAGAAGGAGCCTTCTCC
AATTTTCCTATTTCTGAAGAGACTATAAAGCTTCTGAAAGGTCGAGGGGTAACATATCTC
TTTCCTATTCAAGTTAAGACCTTTGGTCCTGTATATGAAGGAAAAGATTTAATAGCTCAA
GCACGGACAGGAACAGGAAAGACATTCTCTTTTGCCATCCCCTTAATTGAAAGACTCCAA
AGAAATCAAGAAACAATTAAAAAAAGCCGCTCACCAAAGGTACTTGTTTTGGCTCCAACA
AGGGAACTGGCAAACCAAGTAGCCAAAGACTTCAAAGATATAACTAGGAAACTCAGCGTG
GCGTGTTTTTATGGTGGAACATCATATCAAAGCCAAATTAATCATATTCGAAATGGTATT
GACATCTTGGTTGGAACACCTGGTCGTATCAAAGACCATCTGCAGAGTGGCCGATTGGAT
CTTTCTAAACTGCGACATGTTGTGCTTGATGAAGTGGATCAGATGTTAGATTTAGGTTTC
GCTGAACAAGTTGAAGATATTATTCATGAATCCTACAAAACTGATTCTGAAGACAATCCT
CAGACTTTACTTTTTTCTGCAACTTGCCCACAGTGGGTATACAAAGTTGCAAAAAAATAC
ATGAAATCCAGATATGAACAGGTTGACCTTGTTGGAAAAATGACTCAAAAGGCTGCAACT
ACTGTGGAACATTTGGCCATCCAGTGTCATTGGTCTCAGAGGCCAGCAGTTATTGGAGAT
GTCCTTCAAGTCTACAGTGGGTCTGAAGGGAGGGCTATTATTTTCTGTGAGACCAAGAAG
AATGTAACTGAAATGGCCATGAATCCACACATAAAACAGAATGCCCAGTGTTTACATGGG
GACATTGCACAGTCACAAAGAGAAATTACACTAAAAGGCTTCAGAGAAGGTAGTTTTAAA
GTTTTGGTGGCAACCAATGTGGCTGCCCGTGGTTTGGACATTCCTGAAGTTGACCTGGTG
ATTCAAAGTTCTCCTCCTCAGGATGTTGAGTCCTATATCCATCGCTCTGGACGCACAGGT
AGAGCTGGACGGACAGGGATTTGTATATGTTTTTATCAACCAAGAGAAAGAGGTCAACTA
AGATATGTGGAACAAAAAGCAGGAATTACTTTTAAACGTGTAGGTGTTCCTTCTACAATG
GATTTAGTTAAATCTAAAAGCATGGATGCCATCAGGTCTCTGGCTTCCGTTTCTTATGCT
GCTGTTGATTTTTTCCGACCATCAGCTCAGAGACTGATAGAAGAGAAAGGTGCAGTGGAT
GCATTGGCTGCAGCTTTAGCCCACATTTCTGGTGCATCAAGCTTTGAACCACGATCTTTG
ATCACCTCTGATAAGGGGTTTGTGACCATGACTCTGGAAAGCCTAGAGGAAATACAGGAT
GTCAGCTGTGCTTGGAAAGAACTTAACAGAAAGCTGAGTAGTAATGCAGTGTCTCAGATT
ACCAGAATGTGCCTCCTGAAAGGAAATATGGGTGTTTGCTTTGATGTTCCTACAACTGAG
TCAGAAAGGTTACAGGCAGAGTGGCATGATTCCGACTGGATACTCTCAGTGCCAGCCAAA
TTACCTGAAATTGAAGAATATTATGATGGAAACACATCTTCTAATTCCAGACAGAGGAGT
GGCTGGTCAAGTGGTCGATCAGGCCGGTCAGGCCGGTCAGGTGGTCGATCTGGCGGCCGG
TCAGGTAGACAGAGTCGACAAGGAAGTCGCTCAGGAAGTCGACAAGATGGTAGAAGACGA
AGTGGGAATAGAAATCGATCAAGAAGTGGGGGCCACAAACGGAGTTTTGAC

ORF Protein

Cloned ORF protein sequence for pFN21AE5708 Download

>KIEE5708  737 aa
MPGKLLWGDIMELEAPLEESESQKKERQKSDRRKSRHHYDSDEKSETRENGVTDDLDAPK
AKKSKMKEKLNGDTEEGFNRLSDEFSKSHKSRRKDLPNGDIDEYEKKSKRVSSLDTSTHK
SSDNKLEETLTREQKEGAFSNFPISEETIKLLKGRGVTYLFPIQVKTFGPVYEGKDLIAQ
ARTGTGKTFSFAIPLIERLQRNQETIKKSRSPKVLVLAPTRELANQVAKDFKDITRKLSV
ACFYGGTSYQSQINHIRNGIDILVGTPGRIKDHLQSGRLDLSKLRHVVLDEVDQMLDLGF
AEQVEDIIHESYKTDSEDNPQTLLFSATCPQWVYKVAKKYMKSRYEQVDLVGKMTQKAAT
TVEHLAIQCHWSQRPAVIGDVLQVYSGSEGRAIIFCETKKNVTEMAMNPHIKQNAQCLHG
DIAQSQREITLKGFREGSFKVLVATNVAARGLDIPEVDLVIQSSPPQDVESYIHRSGRTG
RAGRTGICICFYQPRERGQLRYVEQKAGITFKRVGVPSTMDLVKSKSMDAIRSLASVSYA
AVDFFRPSAQRLIEEKGAVDALAAALAHISGASSFEPRSLITSDKGFVTMTLESLEEIQD
VSCAWKELNRKLSSNAVSQITRMCLLKGNMGVCFDVPTTESERLQAEWHDSDWILSVPAK
LPEIEEYYDGNTSSNSRQRSGWSSGRSGRSGRSGGRSGGRSGRQSRQGSRSGSRQDGRRR
SGNRNRSRSGGHKRSFD

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE5708 Download

>pFN21AE5708  6916 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGCCTGGGAAACTCCTCTGGGGGGACATTATGGA
GCTGGAAGCACCCTTGGAGGAGTCCGAGAGCCAGAAGAAGGAGAGGCAAAAGAGTGACAG
AAGGAAGTCAAGGCACCATTATGACTCGGATGAGAAATCAGAAACAAGAGAAAATGGTGT
TACAGATGACCTGGATGCTCCCAAGGCCAAAAAATCTAAAATGAAAGAGAAGCTAAATGG
AGACACTGAAGAAGGATTTAATAGACTTTCAGATGAATTCTCCAAATCTCATAAGTCAAG
AAGAAAAGATCTACCAAATGGAGATATAGATGAATATGAAAAAAAATCAAAGCGAGTATC
ATCTTTAGATACTTCTACTCATAAATCAAGTGATAATAAACTAGAGGAGACCTTAACACG
TGAACAGAAAGAAGGAGCCTTCTCCAATTTTCCTATTTCTGAAGAGACTATAAAGCTTCT
GAAAGGTCGAGGGGTAACATATCTCTTTCCTATTCAAGTTAAGACCTTTGGTCCTGTATA
TGAAGGAAAAGATTTAATAGCTCAAGCACGGACAGGAACAGGAAAGACATTCTCTTTTGC
CATCCCCTTAATTGAAAGACTCCAAAGAAATCAAGAAACAATTAAAAAAAGCCGCTCACC
AAAGGTACTTGTTTTGGCTCCAACAAGGGAACTGGCAAACCAAGTAGCCAAAGACTTCAA
AGATATAACTAGGAAACTCAGCGTGGCGTGTTTTTATGGTGGAACATCATATCAAAGCCA
AATTAATCATATTCGAAATGGTATTGACATCTTGGTTGGAACACCTGGTCGTATCAAAGA
CCATCTGCAGAGTGGCCGATTGGATCTTTCTAAACTGCGACATGTTGTGCTTGATGAAGT
GGATCAGATGTTAGATTTAGGTTTCGCTGAACAAGTTGAAGATATTATTCATGAATCCTA
CAAAACTGATTCTGAAGACAATCCTCAGACTTTACTTTTTTCTGCAACTTGCCCACAGTG
GGTATACAAAGTTGCAAAAAAATACATGAAATCCAGATATGAACAGGTTGACCTTGTTGG
AAAAATGACTCAAAAGGCTGCAACTACTGTGGAACATTTGGCCATCCAGTGTCATTGGTC
TCAGAGGCCAGCAGTTATTGGAGATGTCCTTCAAGTCTACAGTGGGTCTGAAGGGAGGGC
TATTATTTTCTGTGAGACCAAGAAGAATGTAACTGAAATGGCCATGAATCCACACATAAA
ACAGAATGCCCAGTGTTTACATGGGGACATTGCACAGTCACAAAGAGAAATTACACTAAA
AGGCTTCAGAGAAGGTAGTTTTAAAGTTTTGGTGGCAACCAATGTGGCTGCCCGTGGTTT
GGACATTCCTGAAGTTGACCTGGTGATTCAAAGTTCTCCTCCTCAGGATGTTGAGTCCTA
TATCCATCGCTCTGGACGCACAGGTAGAGCTGGACGGACAGGGATTTGTATATGTTTTTA
TCAACCAAGAGAAAGAGGTCAACTAAGATATGTGGAACAAAAAGCAGGAATTACTTTTAA
ACGTGTAGGTGTTCCTTCTACAATGGATTTAGTTAAATCTAAAAGCATGGATGCCATCAG
GTCTCTGGCTTCCGTTTCTTATGCTGCTGTTGATTTTTTCCGACCATCAGCTCAGAGACT
GATAGAAGAGAAAGGTGCAGTGGATGCATTGGCTGCAGCTTTAGCCCACATTTCTGGTGC
ATCAAGCTTTGAACCACGATCTTTGATCACCTCTGATAAGGGGTTTGTGACCATGACTCT
GGAAAGCCTAGAGGAAATACAGGATGTCAGCTGTGCTTGGAAAGAACTTAACAGAAAGCT
GAGTAGTAATGCAGTGTCTCAGATTACCAGAATGTGCCTCCTGAAAGGAAATATGGGTGT
TTGCTTTGATGTTCCTACAACTGAGTCAGAAAGGTTACAGGCAGAGTGGCATGATTCCGA
CTGGATACTCTCAGTGCCAGCCAAATTACCTGAAATTGAAGAATATTATGATGGAAACAC
ATCTTCTAATTCCAGACAGAGGAGTGGCTGGTCAAGTGGTCGATCAGGCCGGTCAGGCCG
GTCAGGTGGTCGATCTGGCGGCCGGTCAGGTAGACAGAGTCGACAAGGAAGTCGCTCAGG
AAGTCGACAAGATGGTAGAAGACGAAGTGGGAATAGAAATCGATCAAGAAGTGGGGGCCA
CAAACGGAGTTTTGACGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTC
GACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGG
CTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGAC
ATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGC
TTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAA
CAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAG
GTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTC
TTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAG
CACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTT
CGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTAT
CCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATG
AGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTT
TTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGA
GTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAA
GAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGT
ATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTT
GAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGC
AGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGA
GGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGAT
CGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCT
GTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCC
CGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCG
GCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGC
GGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACG
ACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCA
CTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTC
ACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGT
GAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCC
ATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAA
ACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTC
CTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGG
CGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGC
TGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATC
GTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACA
GGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACT
ACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCG
GAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTT
TTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCT
TTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGA
GATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACG
CTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAG
TGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATC
AGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGT
GATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTA
CTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCG
TAGAGGATCGAGATCT