(Linear) (Six-base) MAPSORT of: KIEE5708.seq Check: 6279 from: 1 to: 2211 KIEE5708.orf With 182 enzymes: SgfI * December 5, 2014 13:07 .. AccI GT'mk_AC Cuts at: 0 998 1151 2106 2115 2139 2211 Size: 998 153 955 9 24 72 Fragments arranged by size: 998 955 153 72 24 9 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 242 455 968 1184 1284 1385 2211 Size: 242 213 513 216 100 101 826 Fragments arranged by size: 826 513 242 216 213 101 100 AflIII A'CryG_T Cuts at: 0 391 855 1536 2211 Size: 391 464 681 675 Fragments arranged by size: 681 675 464 391 AhdI GACnn_n'nnGTC Cuts at: 0 503 2211 Size: 503 1708 AlwNI CAG_nnn'CTG Cuts at: 0 1653 2211 Size: 1653 558 ApoI r'AATT_y Cuts at: 0 250 2211 Size: 250 1961 AseI AT'TA_AT Cuts at: 0 758 2211 Size: 758 1453 BanI G'GyrC_C Cuts at: 0 107 2211 Size: 107 2104 BbsI GAAGACnn'nnnn_ Cuts at: 0 956 2160 2211 Size: 956 1204 51 Fragments arranged by size: 1204 956 51 BciVI GTATCCnnnnn_n' Cuts at: 0 1952 2211 Size: 1952 259 BclI T'GATC_A Cuts at: 0 1739 2211 Size: 1739 472 BfrBI ATG'CAT Cuts at: 0 1681 2211 Size: 1681 530 BglI GCCn_nnn'nGGC Cuts at: 0 2069 2211 Size: 2069 142 BglII A'GATC_T Cuts at: 0 282 2211 Size: 282 1929 BmrI ACTGGGnnnn_n' Cuts at: 0 1239 2211 Size: 1239 972 Bpu10I CC'TnA_GC Cuts at: 0 2130 2211 Size: 2130 81 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 521 2211 Size: 521 1690 BsaI GGTCTCn'nnnn_ Cuts at: 0 376 1118 1183 1601 2211 Size: 376 742 65 418 610 Fragments arranged by size: 742 610 418 376 65 BsaAI yAC'GTr Cuts at: 0 394 2211 Size: 394 1817 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 4 67 394 1382 1385 2049 2211 Size: 4 63 327 988 3 664 162 Fragments arranged by size: 988 664 327 162 63 4 3 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1691 2211 Size: 1691 520 BsiEI CG_ry'CG Cuts at: 0 2097 2211 Size: 2097 114 BsmI GAATG_Cn' Cuts at: 0 1245 2211 Size: 1245 966 BspHI T'CATG_A Cuts at: 0 924 2211 Size: 924 1287 BsrBI CCG'CTC Cuts at: 0 629 2211 Size: 629 1582 BsrDI GCAATG_nn' Cuts at: 0 1262 2211 Size: 1262 949 BsrFI r'CCGG_y Cuts at: 0 2063 2072 2096 2211 Size: 2063 9 24 115 Fragments arranged by size: 2063 115 24 9 BstAPI GCAn_nnn'nTGC Cuts at: 0 1678 1687 2211 Size: 1678 9 524 Fragments arranged by size: 1678 524 9 BstBI TT'CG_AA Cuts at: 0 768 2211 Size: 768 1443 BstXI CCAn_nnnn'nTGG Cuts at: 0 1426 1708 1773 2211 Size: 1426 282 65 438 Fragments arranged by size: 1426 438 282 65 BstYI r'GATC_y Cuts at: 0 282 837 2211 Size: 282 555 1374 Fragments arranged by size: 1374 555 282 BstZ17I GTA'TAC Cuts at: 0 999 2211 Size: 999 1212 Bsu36I CC'TnA_GG Cuts at: 0 1397 2211 Size: 1397 814 BtgI C'CryG_G Cuts at: 0 1347 2211 Size: 1347 864 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1405 2211 Size: 1405 806 BtsI GCAGTG_nn' Cuts at: 0 1679 1853 2211 Size: 1679 174 358 Fragments arranged by size: 1679 358 174 ClaI AT'CG_AT Cuts at: 0 2175 2211 Size: 2175 36 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1064 1099 1319 1354 1718 1753 2211 Size: 1064 35 220 35 364 35 458 Fragments arranged by size: 1064 458 364 220 35 35 35 DraI TTT'AAA Cuts at: 0 1317 1533 2211 Size: 1317 216 678 Fragments arranged by size: 1317 678 216 DrdI GACnn_nn'nnGTC Cuts at: 0 2123 2211 Size: 2123 88 EaeI y'GGCC_r Cuts at: 0 828 1094 1214 2094 2211 Size: 828 266 120 880 117 Fragments arranged by size: 880 828 266 120 117 EagI C'GGCC_G Cuts at: 0 2094 2211 Size: 2094 117 EarI CTCTTCn'nnn_ Cuts at: 0 431 1655 2211 Size: 431 1224 556 Fragments arranged by size: 1224 556 431 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 242 455 968 1184 1284 1385 2211 Size: 242 213 513 216 100 101 826 Fragments arranged by size: 826 513 242 216 213 101 100 EcoRI G'AATT_C Cuts at: 0 250 2211 Size: 250 1961 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 115 147 238 270 489 521 1393 1425 Size: 115 32 91 32 219 32 872 32 Cuts at: 1425 1724 1755 1756 1787 2211 Size: 299 31 1 31 424 Fragments arranged by size: 872 424 299 219 115 91 32 32 32 32 31 31 1 HincII GTy'rAC Cuts at: 0 1044 1371 1495 2116 2140 2211 Size: 1044 327 124 621 24 71 Fragments arranged by size: 1044 621 327 124 71 24 HindIII A'AGCT_T Cuts at: 0 448 1719 2211 Size: 448 1271 492 Fragments arranged by size: 1271 492 448 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 679 771 1032 1659 1976 2211 Size: 679 92 261 627 317 235 Fragments arranged by size: 679 627 317 261 235 92 MscI TGG'CCA Cuts at: 0 1096 1216 2211 Size: 1096 120 995 Fragments arranged by size: 1096 995 120 MslI CAynn'nnrTG Cuts at: 0 113 2211 Size: 113 2098 MspA1I CmG'CkG Cuts at: 0 1805 2211 Size: 1805 406 NsiI A_TGCA'T Cuts at: 0 1683 2211 Size: 1683 528 NspI r_CATG'y Cuts at: 0 859 2211 Size: 859 1352 PciI A'CATG_T Cuts at: 0 855 2211 Size: 855 1356 PmlI CAC'GTG Cuts at: 0 394 2211 Size: 394 1817 PstI C_TGCA'G Cuts at: 0 824 1692 2211 Size: 824 868 519 Fragments arranged by size: 868 824 519 PvuII CAG'CTG Cuts at: 0 1805 2211 Size: 1805 406 SalI G'TCGA_C Cuts at: 0 2114 2138 2211 Size: 2114 24 73 Fragments arranged by size: 2114 73 24 SexAI A'CCwGG_T Cuts at: 0 798 1373 2211 Size: 798 575 838 Fragments arranged by size: 838 798 575 SfcI C'TryA_G Cuts at: 0 820 1152 1688 2211 Size: 820 332 536 523 Fragments arranged by size: 820 536 523 332 SfiI GGCCn_nnn'nGGCC Cuts at: 0 2069 2211 Size: 2069 142 SmlI C'TyrA_G Cuts at: 0 536 2211 Size: 536 1675 SspI AAT'ATT Cuts at: 0 1999 2211 Size: 1999 212 StyI C'CwwG_G Cuts at: 0 47 176 2211 Size: 47 129 2035 Fragments arranged by size: 2035 129 47 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1879 2211 Size: 1879 332 Tth111I GACn'n_nGTC Cuts at: 0 2111 2211 Size: 2111 100 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 823 1252 1596 2211 Size: 823 429 344 615 Fragments arranged by size: 823 615 429 344 Enzymes that do cut: AccI AcuI AflIII AhdI AlwNI ApoI AseI BanI BbsI BciVI BclI BfrBI BglI BglII BmrI Bpu10I BpuEI BsaI BsaAI BseRI BsgI BsiEI BsmI BspHI BsrBI BsrDI BsrFI BstAPI BstBI BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI Eco57MI EcoRI Hin4I HincII HindIII MmeI MscI MslI MspA1I NsiI NspI PciI PmlI PstI PvuII SalI SexAI SfcI SfiI SmlI SspI StyI TaqII Tth111I XcmI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AgeI AleI AlfI AloI ApaI ApaLI AscI AsiSI AvaI AvrII BaeI BamHI BanII BbeI BbvCI BcgI BlpI Bme1580I BmgBI BmtI BplI BpmI BsaBI BsaHI BsaWI BsaXI BseYI BsiHKAI BsiWI BsmBI Bsp1286I BspEI BspMI BsrGI BssHII BssSI BstEII DraIII EciI EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspI FspAI HaeII HpaI KasI KpnI MfeI MluI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI PacI PasI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII SanDI SapI SbfI ScaI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI SwaI TatI TstI XbaI XhoI XmaI XmnI ZraI