RNF150 ORF clone FHC00768

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC00768

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Clone info.

Species

human

Clone Name

pFN21ASDA1214 Sequence (with vector) Restriction Map

Product ID

FHC00768

Flexi Clone PID

FXC00768

ORF Sequence

DNA sequence (1188 bp)
Protein sequence (396 aa)

Linker Type

Flexi_RBS type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB384141]

Gene

KIAA1214

Symbol

RNF150

Description

ring finger protein 150

Original Clone ID

fh01431

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1188 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 396 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS34065.1 3.3e-71 90.2 RNF150 CCDS4451.1 1.5e-27 45.4 RNF130 CCDS64340.1 2.1e-27 45.7 RNF130 CCDS14520.1 1.2e-19 39.4 RNF128 CCDS14521.1 1.3e-19 38.3 RNF128 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR003137 85 181 PF02225 Protease-associated domain IPR018957 236 276 PF00097 Zinc finger HMMSmart IPR001841 236 276 SM00184 Zinc finger ProfileScan IPR001841 236 277 PS50089 Zinc finger
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 7 QACCSLALSTWLLSFCFVHLLCL 29 PRIMARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_078815 402 300439 39.4 86.9 NP_919445 428 300439 38.3 86.6 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21ASDA1214 Download

>KIAA1214  1188 bp
ATGGCAATGTCTCTCATCCAAGCGTGCTGCAGTCTGGCTCTCTCAACATGGCTGCTTTCC
TTTTGTTTCGTGCATCTGCTCTGCCTGGACTTTACCGTGGCCGAGAAGGAGGAATGGTAC
ACCGCCTTCGTGAACATCACCTACGCCGAGCCCGCGCCGGACCCCGGGGCCGGGGCGGCG
GGCGGCGGCGGCGCGGAGCTGCACACGGAGAAGACGGAGTGCGGGCGCTACGGAGAGCAC
TCGCCCAAGCAGGACGCCCGCGGGGAGGTGGTCATGGCCAGCTCGGCCCACGACCGCCTG
GCCTGCGACCCCAACACCAAGTTCGCCGCCCCGACCCGCGGCAAGAACTGGATAGCCCTC
ATCCCCAAGGGCAACTGCACGTACAGGGATAAGATCCGGAACGCGTTCCTGCAGAACGCC
TCAGCCGTGGTCATCTTCAACGTGGGCTCCAACACCAACGAGACCATCACCATGCCCCAC
GCGGGTGTAGAAGACATCGTGGCCATAATGATTCCTGAGCCAAAAGGGAAGGAGATAGTA
AGCCTGCTGGAAAGAAACATCACCGTGACAATGTACATCACCATCGGAACCCGGAACTTG
CAGAAATATCGCCGACTGGGGGATGCAGCAAAGAAAGCCATCAGCAAACTCCAGATCAGG
ACCATCAAGAAGGGTGACAAGGAAACAGAGTCTGATTTTGACAACTGTGCAGTTTGTATT
GAAGGGTACAAGCCCAATGACGTTGTCCGGATCCTGCCCTGCCGGCATCTTTTCCACAAG
TCCTGTGTTGACCCCTGGCTTCTAGACCATCGTACCTGTCCCATGTGCAAGATGAACATT
CTTAAAGCCCTAGGGATCCCGCCCAATGCCGACTGCATGGACGACTTGCCCACTGACTTC
GAGGGCTCTCTGGGAGGTCCACCCACCAACCAGATCACAGGTGCCAGCGACACAACAGTG
AATGAAAGTTCAGTCACTTTGGACCCTGCTGTCCGGACTGTGGGAGCCTTGCAGGTGGTC
CAGGATACAGACCCCATCCCCCAGGAGGGAGACGTCATCTTTACTACTAACAGTGAGCAG
GAGCCAGCTGTAAGCAGTGATTCTGACATTTCCTTGATCATGGCAATGGAGGTTGGACTG
TCTGATGTAGAACTTTCCACTGACCAGGACTGTGAAGAAGTGAAATCT

ORF Protein

Cloned ORF protein sequence for pFN21ASDA1214 Download

>KIAA1214  396 aa
MAMSLIQACCSLALSTWLLSFCFVHLLCLDFTVAEKEEWYTAFVNITYAEPAPDPGAGAA
GGGGAELHTEKTECGRYGEHSPKQDARGEVVMASSAHDRLACDPNTKFAAPTRGKNWIAL
IPKGNCTYRDKIRNAFLQNASAVVIFNVGSNTNETITMPHAGVEDIVAIMIPEPKGKEIV
SLLERNITVTMYITIGTRNLQKYRRLGDAAKKAISKLQIRTIKKGDKETESDFDNCAVCI
EGYKPNDVVRILPCRHLFHKSCVDPWLLDHRTCPMCKMNILKALGIPPNADCMDDLPTDF
EGSLGGPPTNQITGASDTTVNESSVTLDPAVRTVGALQVVQDTDPIPQEGDVIFTTNSEQ
EPAVSSDSDISLIMAMEVGLSDVELSTDQDCEEVKS

ORF+Vector

Nucleotide Sequence (with vector) for pFN21ASDA1214 Download

>pFN21ASDA1214  5917 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCTTTCGAAGGAGATAGAACCATGGCAATGTCTCTCAT
CCAAGCGTGCTGCAGTCTGGCTCTCTCAACATGGCTGCTTTCCTTTTGTTTCGTGCATCT
GCTCTGCCTGGACTTTACCGTGGCCGAGAAGGAGGAATGGTACACCGCCTTCGTGAACAT
CACCTACGCCGAGCCCGCGCCGGACCCCGGGGCCGGGGCGGCGGGCGGCGGCGGCGCGGA
GCTGCACACGGAGAAGACGGAGTGCGGGCGCTACGGAGAGCACTCGCCCAAGCAGGACGC
CCGCGGGGAGGTGGTCATGGCCAGCTCGGCCCACGACCGCCTGGCCTGCGACCCCAACAC
CAAGTTCGCCGCCCCGACCCGCGGCAAGAACTGGATAGCCCTCATCCCCAAGGGCAACTG
CACGTACAGGGATAAGATCCGGAACGCGTTCCTGCAGAACGCCTCAGCCGTGGTCATCTT
CAACGTGGGCTCCAACACCAACGAGACCATCACCATGCCCCACGCGGGTGTAGAAGACAT
CGTGGCCATAATGATTCCTGAGCCAAAAGGGAAGGAGATAGTAAGCCTGCTGGAAAGAAA
CATCACCGTGACAATGTACATCACCATCGGAACCCGGAACTTGCAGAAATATCGCCGACT
GGGGGATGCAGCAAAGAAAGCCATCAGCAAACTCCAGATCAGGACCATCAAGAAGGGTGA
CAAGGAAACAGAGTCTGATTTTGACAACTGTGCAGTTTGTATTGAAGGGTACAAGCCCAA
TGACGTTGTCCGGATCCTGCCCTGCCGGCATCTTTTCCACAAGTCCTGTGTTGACCCCTG
GCTTCTAGACCATCGTACCTGTCCCATGTGCAAGATGAACATTCTTAAAGCCCTAGGGAT
CCCGCCCAATGCCGACTGCATGGACGACTTGCCCACTGACTTCGAGGGCTCTCTGGGAGG
TCCACCCACCAACCAGATCACAGGTGCCAGCGACACAACAGTGAATGAAAGTTCAGTCAC
TTTGGACCCTGCTGTCCGGACTGTGGGAGCCTTGCAGGTGGTCCAGGATACAGACCCCAT
CCCCCAGGAGGGAGACGTCATCTTTACTACTAACAGTGAGCAGGAGCCAGCTGTAAGCAG
TGATTCTGACATTTCCTTGATCATGGCAATGGAGGTTGGACTGTCTGATGTAGAACTTTC
CACTGACCAGGACTGTGAAGAAGTGAAATCTTACGTAGTTTAAACGAATTCGGGCTCGGT
ACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAG
CCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTG
GGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACT
AGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTA
ACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAG
GTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAA
ATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAG
CTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCT
TAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTC
TAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAA
TATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTT
GCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCT
GAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATC
CTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTA
TGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACAC
TATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGC
ATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAAC
TTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGG
GATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGAC
GAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGC
GAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTT
GCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGA
GCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCC
CGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAG
ATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCG
ACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGG
ATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGG
CCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGAC
GCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTG
GAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCT
TTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGG
TGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCT
GCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCAC
TGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGT
TCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTC
TGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCA
CCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGAT
TTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCAC
GTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGT
CCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAA
TGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTA
TTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCA
TTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAG
GGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACT
CCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT