Restriction map for
pFN21ASDA1214
(Circular) (Six-base) MAPSORT of: pFN21ASDA1214.seq Check: 2267 from: 1 to: 5917
pFN21ASDA1214 5917 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3025 3025
Size: 5917
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3078 3727 278
Size: 53 83 186 936 1542 649 2468
Fragments arranged by size:
2468 1542 936 649 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3260 1937
Size: 1323 4594
Acc65I G'GTAC_C
Cuts at: 3238 3238
Size: 5917
AclI AA'CG_TT
Cuts at: 1563 4044 4417 1563
Size: 2481 373 3063
Fragments arranged by size:
3063 2481 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3980 5364 5632 1632
Size: 2348 1384 268 1917
Fragments arranged by size:
2348 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5720
AflIII A'CryG_T
Cuts at: 1203 2424 4816 5793 1203
Size: 1221 2392 977 1327
Fragments arranged by size:
2392 1327 1221 977
AgeI A'CCGG_T
Cuts at: 4748 4748
Size: 5917
AhdI GACnn_n'nnGTC
Cuts at: 4646 4646
Size: 5917
AleI CACnn'nnGTG
Cuts at: 1166 2505 2978 1166
Size: 1339 473 4105
Fragments arranged by size:
4105 1339 473
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5917
AlwNI CAG_nnn'CTG
Cuts at: 2714 3660 5232 2714
Size: 946 1572 3399
Fragments arranged by size:
3399 1572 946
ApaLI G'TGCA_C
Cuts at: 3974 5130 3974
Size: 1156 4761
ApoI r'AATT_y
Cuts at: 1493 3226 3452 3604 5713 1493
Size: 1733 226 152 2109 1697
Fragments arranged by size:
2109 1733 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4471 160
Size: 872 3439 1606
Fragments arranged by size:
3439 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5917
AvaI C'yCGr_G
Cuts at: 1870 1943 2186 3242 1870
Size: 73 243 1056 4545
Fragments arranged by size:
4545 1056 243 73
AvrII C'CTAG_G
Cuts at: 2872 2872
Size: 5917
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2818 2851 2818
Size: 33 5884
BamHI G'GATC_C
Cuts at: 2772 2877 3247 3614 2772
Size: 105 370 367 5075
Fragments arranged by size:
5075 370 367 105
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2963 3238 4693 618
Size: 325 217 627 1176 275 1455 1842
Fragments arranged by size:
1842 1455 1176 627 325 275 217
BanII G_rGCy'C
Cuts at: 1391 2175 2471 2930 3236 1391
Size: 784 296 459 306 4072
Fragments arranged by size:
4072 784 459 306 296
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2240 2520 928
Size: 971 341 280 4325
Fragments arranged by size:
4325 971 341 280
BbvCI CC'TCA_GC
Cuts at: 2443 2443
Size: 5917
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2612 2646 4108 4142 1903
Size: 34 675 34 1462 34 3678
Fragments arranged by size:
3678 1462 675 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3040 3809 5019 3040
Size: 769 1210 3938
Fragments arranged by size:
3938 1210 769
BclI T'GATC_A
Cuts at: 1548 3138 1548
Size: 1590 4327
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2198 2306 2785
Size: 107 122 71 631 605 526 108 479
Cuts at: 2785 4528 136
Size: 1743 1525
Fragments arranged by size:
1743 1525 631 605 526 479 122 108
107 71
BglII A'GATC_T
Cuts at: 5912 5912
Size: 5917
BlpI GC'TnA_GC
Cuts at: 3334 3334
Size: 5917
Bme1580I G_kGCm'C
Cuts at: 1790 3978 5134 1790
Size: 2188 1156 2573
Fragments arranged by size:
2573 2188 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5917
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2648 3648 4606
Size: 416 53 83 399 24 1231 1000 958
Cuts at: 4606 442
Size: 1753
Fragments arranged by size:
1753 1231 1000 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5917
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2657 4577 1239
Size: 1418 1920 2579
Fragments arranged by size:
2579 1920 1418
Bpu10I CC'TnA_GC
Cuts at: 2443 2538 2443
Size: 95 5822
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4042 4907 5205 4042
Size: 865 298 4754
Fragments arranged by size:
4754 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2477 4580 882
Size: 617 978 2103 2219
Fragments arranged by size:
2219 2103 978 617
BsaAI yAC'GTr
Cuts at: 493 1204 2403 3214 3708 493
Size: 711 1199 811 494 2702
Fragments arranged by size:
2702 1199 811 711 494
BsaBI GATnn'nnATC
Cuts at: 5911 5911
Size: 5917
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2277 3075 3724 4106
Size: 53 83 186 936 744 798 649 382
Cuts at: 4106 4741 275
Size: 635 1451
Fragments arranged by size:
1451 936 798 744 649 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2418 2769 3015 4350 4748 5022 5169 5580 2418
Size: 351 246 1335 398 274 147 411 2755
Fragments arranged by size:
2755 1335 411 398 351 274 246 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2123 2153 2453 2483 1178
Size: 30 165 30 720 30 300 30 4612
Fragments arranged by size:
4612 720 300 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5917
BseYI C'CCAG_C
Cuts at: 5120 5616 5120
Size: 496 5421
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2207 2383 2751 1163
Size: 573 471 176 368 4329
Fragments arranged by size:
4329 573 471 368 176
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2317 3367 4128 5156 1540
Size: 275 186 316 1050 761 1028 2301
Fragments arranged by size:
2301 1050 1028 761 316 275 186
BsiHKAI G_wGCw'C
Cuts at: 2262 3978 4063 5134 2262
Size: 1716 85 1071 3045
Fragments arranged by size:
3045 1716 1071 85
BsmI GAATG_Cn'
Cuts at: 3428 3521 3428
Size: 93 5824
BsmBI CGTCTCn'nnnn_
Cuts at: 3066 3066
Size: 5917
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2175 2262 2471 2930 3236 3978 4063
Size: 399 385 87 209 459 306 742 85
Cuts at: 4063 5134 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 459 399 385 306 209
87 85
BspEI T'CCGG_A
Cuts at: 2418 2769 3015 5580 2418
Size: 351 246 2565 2755
Fragments arranged by size:
2755 2565 351 246
BspHI T'CATG_A
Cuts at: 3806 5536 3806
Size: 1730 4187
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3025 3272 844
Size: 2181 247 3489
Fragments arranged by size:
3489 2181 247
BsrBI CCG'CTC
Cuts at: 1394 1823 3804 1394
Size: 429 1981 3507
Fragments arranged by size:
3507 1981 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2034 3153 4412 4586 65
Size: 1183 786 1119 1259 174 1396
Fragments arranged by size:
1396 1259 1183 1119 786 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2784 4561 4748 1700
Size: 110 974 1777 187 2869
Fragments arranged by size:
2869 1777 974 187 110
BsrGI T'GTAC_A
Cuts at: 96 2595 96
Size: 2499 3418
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5917
BssSI C'ACGA_G
Cuts at: 3977 4989 3977
Size: 1012 4905
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5917
BstBI TT'CG_AA
Cuts at: 2007 4721 2007
Size: 2714 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5917
BstYI r'GATC_y
Cuts at: 1972 2415 2772 2877 3247 3614 3999 4016 5468
Size: 443 357 105 370 367 385 17 1452
Cuts at: 5468 5554 5566 5912 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 443 385 370 367 357 346
105 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 2118 2281 2359 2448 513
Size: 552 957 96 163 78 89 3982
Fragments arranged by size:
3982 957 552 163 96 89 78
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5178
Fragments arranged by size:
5178 683 56
BtsI GCAGTG_nn'
Cuts at: 3124 3433 4227 4247 3124
Size: 309 794 20 4794
Fragments arranged by size:
4794 794 309 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5917
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5882
DraI TTT'AAA
Cuts at: 3221 3221
Size: 5917
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3011 4924 809
Size: 507 1695 1913 1802
Fragments arranged by size:
1913 1802 1695 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2121 2298 2523
Size: 54 1425 50 275 18 291 177 225
Cuts at: 2523 3364 4253 5882 8
Size: 841 889 1629 43
Fragments arranged by size:
1629 1425 889 841 291 275 225 177
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3364 1537
Size: 275 1552 4090
Fragments arranged by size:
4090 1552 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3847 1353
Size: 2494 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4504 4878 5024 387
Size: 1064 138 2915 374 146 1280
Fragments arranged by size:
2915 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2657 3980 4577 5364 5632 1239
Size: 393 1025 1323 597 787 268 1524
Fragments arranged by size:
1524 1323 1025 787 597 393 268
EcoRI G'AATT_C
Cuts at: 3226 3604 3226
Size: 378 5539
FspI TGC'GCA
Cuts at: 4423 4423
Size: 5917
HaeII r_GCGC'y
Cuts at: 1678 2251 5064 1678
Size: 573 2813 2531
Fragments arranged by size:
2813 2531 573
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3063 3095 4558 4590 4632
Size: 32 135 32 1491 32 1463 32 42
Cuts at: 4632 4664 1373
Size: 32 2626
Fragments arranged by size:
2626 1491 1463 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2812 3261 3507 669
Size: 988 281 874 449 246 3079
Fragments arranged by size:
3079 988 874 449 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5917
HpaI GTT'AAC
Cuts at: 3507 3507
Size: 5917
KpnI G_GTAC'C
Cuts at: 3242 3242
Size: 5917
MfeI C'AATT_G
Cuts at: 3516 3516
Size: 5917
MluI A'CGCG_T
Cuts at: 2424 5793 2424
Size: 3369 2548
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2496 3136 5031 5215 1305
Size: 1191 640 1895 184 2007
Fragments arranged by size:
2007 1895 1191 640 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2300 2525 10
Size: 54 1425 343 468 225 3402
Fragments arranged by size:
3402 1425 468 343 225 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2027 2492 2505 2585 2978 3146 3875
Size: 648 861 465 13 80 393 168 729
Cuts at: 3875 4234 4393 518
Size: 359 159 2042
Fragments arranged by size:
2042 861 729 648 465 393 359 168
159 80 13
MspA1I CmG'CkG
Cuts at: 1606 2283 2361 3110 3333 4010 5158 5403 1606
Size: 677 78 749 223 677 1148 245 2120
Fragments arranged by size:
2120 1148 749 677 677 245 223 78
NaeI GCC'GGC
Cuts at: 2786 2786
Size: 5917
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 4408
Fragments arranged by size:
4408 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5917
NgoMIV G'CCGG_C
Cuts at: 2784 2784
Size: 5917
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5917
NotI GC'GGCC_GC
Cuts at: 3364 3364
Size: 5917
NspI r_CATG'y
Cuts at: 1132 1229 3275 4820 1132
Size: 97 2046 1545 2229
Fragments arranged by size:
2229 2046 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5917
PciI A'CATG_T
Cuts at: 4816 4816
Size: 5917
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5917
PfoI T'CCnGG_A
Cuts at: 3042 3042
Size: 5917
PmeI GTTT'AAAC
Cuts at: 3221 3221
Size: 5917
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3986 4018 5499 5531 3986
Size: 32 1481 32 4372
Fragments arranged by size:
4372 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5917
PsiI TTA'TAA
Cuts at: 3487 3487
Size: 5917
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2147 2179 2147
Size: 32 5885
PstI C_TGCA'G
Cuts at: 830 2054 2436 3269 830
Size: 1224 382 833 3478
Fragments arranged by size:
3478 1224 833 382
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5917
PvuII CAG'CTG
Cuts at: 3110 3110
Size: 5917
SacII CC_GC'GG
Cuts at: 2284 2362 2284
Size: 78 5839
SalI G'TCGA_C
Cuts at: 1936 3259 1936
Size: 1323 4594
SbfI CC_TGCA'GG
Cuts at: 3269 3269
Size: 5917
ScaI AGT'ACT
Cuts at: 4165 5877 4165
Size: 1712 4205
SfcI C'TryA_G
Cuts at: 826 1045 2050 2432 3265 4400 5081 5272 826
Size: 219 1005 382 833 1135 681 191 1471
Fragments arranged by size:
1471 1135 1005 833 681 382 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2306 2306
Size: 5917
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5917
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5917
SmaI CCC'GGG
Cuts at: 1872 2188 3244 1872
Size: 316 1056 4545
Fragments arranged by size:
4545 1056 316
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4021 4922 5184 820
Size: 197 926 2078 901 262 1553
Fragments arranged by size:
2078 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3214 3708 493
Size: 2721 494 2702
Fragments arranged by size:
2721 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5917
SphI G_CATG'C
Cuts at: 1132 3275 1132
Size: 2143 3774
SspI AAT'ATT
Cuts at: 5 52 3841 5
Size: 47 3789 2081
Fragments arranged by size:
3789 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5917
StyI C'CwwG_G
Cuts at: 513 1065 2022 2388 2872 3356 513
Size: 552 957 366 484 484 3074
Fragments arranged by size:
3074 957 552 484 484 366
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2959 3944 3961 4114 4299 4744 325
Size: 1255 1379 985 17 153 185 445 1498
Fragments arranged by size:
1498 1379 1255 985 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2595 4163 5875
Size: 274 80 33 51 1443 618 1568 1712
Cuts at: 5875 96
Size: 138
Fragments arranged by size:
1712 1568 1443 618 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5877 5909 5877
Size: 32 5885
Tth111I GACn'n_nGTC
Cuts at: 1316 2765 1316
Size: 1449 4468
XbaI T'CTAG_A
Cuts at: 2824 3253 2824
Size: 429 5488
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5917
XmaI C'CCGG_G
Cuts at: 1870 2186 3242 1870
Size: 316 1056 4545
Fragments arranged by size:
4545 1056 316
XmnI GAAnn'nnTTC
Cuts at: 2426 4046 2426
Size: 1620 4297
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3076 3725 276
Size: 53 83 186 936 1542 649 2468
Fragments arranged by size:
2468 1542 936 649 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PsrI PstI PvuI PvuII SacII SalI
SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI BbeI BfrBI BplI BsiWI
BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV
FalI FseI FspAI KasI NarI NruI NsiI PacI
PmlI PpuMI PspOMI PspXI RsrII SacI SanDI SapI
SexAI SfoI SrfI SwaI XcmI