GRAMD1B ORF clone FHC00764

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Halotag ORF Clone FHC00764

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Clone info.

Species

human

Clone Name

pFN21ASDA1201 Sequence (with vector) Restriction Map

Product ID

FHC00764

Flexi Clone PID

FXC00764

ORF Sequence

DNA sequence (2094 bp)
Protein sequence (698 aa)

Linker Type

Flexi_RBS type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB384137]

Gene

KIAA1201

Symbol

GRAMD1B

Alias : LINC01059

Description

GRAM domain containing 1B, transcript variant 3

Original Clone ID

fg03153

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 2094 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 698 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS66254.1 0 100.0 GRAMD1B CCDS53720.1 0 100.0 GRAMD1B CCDS66253.1 0 99.0 GRAMD1B CCDS81640.1 0 99.4 GRAMD1B CCDS42546.1 7.8e-101 47.6 GRAMD1A The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR004182 56 123 PF02893 GRAM HMMSmart IPR004182 56 123 SM00568 GRAM
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 583 LLLVISCVICFSLVLLVILNMML 605 PRIMARY 23 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21ASDA1201 Download

>KIAA1201  2094 bp
ATGGTGGAGAAGGGCTCAGATCACTCCTCGGACAAGTCCCCGTCCACACCGGAGCAGGGC
GTGCAGCGCAGCTGCTCCTCCCAGTCCGGCCGGAGCGGCGGCAAGAATTCCAAGAAAAGC
CAGAGTTGGTATAATGTGTTAAGCCCCACCTACAAGCAGAGAAATGAAGACTTCAGAAAG
CTCTTTAAGCAGCTTCCAGACACGGAGCGCCTCATTGTTGATTACTCATGTGCACTCCAA
AGAGACATTCTCCTTCAGGGCCGACTCTACCTCTCTGAAAATTGGATCTGCTTCTACAGC
AACATCTTCCGCTGGGAAACTCTGCTGACAGTCCGTTTGAAAGACATCTGTTCCATGACT
AAAGAAAAAACAGCTCGCCTCATTCCCAATGCCATCCAAGTTTGCACTGATTCAGAAAAG
CACTTCTTCACTTCGTTTGGGGCCCGGGATAGGACATATATGATGATGTTCCGGCTCTGG
CAGAATGCTCTCCTTGAAAAGCCTCTGTGTCCCAAGGAGCTCTGGCACTTTGTTCACCAG
TGCTATGGGAACGAATTGGGCCTGACCAGTGATGACGAGGACTACGTGCCCCCTGACGAC
GACTTCAACACAATGGGATACTGTGAAGAGATCCCTGTGGAAGAGAATGAAGTGAATGAC
AGCTCATCCAAGAGCAGCATAGAGACCAAGCCAGATGCCAGTCCACAGCTGCCCAAGAAA
TCCATCACCAACAGCACACTAACATCCACAGGGAGCAGTGAGGCCCCCGTCTCGTTTGAT
GGGCTGCCCCTGGAGGAAGAGGCGCTGGAAGGAGACGGGTCCCTGGAAAAGGAGCTCGCC
ATTGACAACATCATGGGGGAGAAGATTGAGATGATCGCTCCTGTGAACTCCCCTTCACTG
GACTTCAATGACAATGAGGACATCCCCACTGAGCTCAGTGACTCTTCCGACACACACGAT
GAAGGAGAGGTCCAGGCCTTCTATGAGGACCTGAGTGGCCGGCAGTACGTGAATGAAGTC
TTCAACTTCAGCGTGGACAAGCTCTATGACCTCCTCTTCACCAACTCGCCCTTCCAGCGG
GATTTCATGGAGCAGCGGCGCTTCTCTGATATCATCTTCCATCCATGGAAAAAGGAGGAG
AATGGAAACCAGAGCCGAGTGATTCTTTACACCATCACCCTTACCAACCCTCTGGCTCCC
AAAACTGCCACTGTCAGGGAGACACAGACCATGTACAAGGCGAGCCAGGAGAGTGAATGT
TACGTGATAGATGCCGAAGTCCTCACCCACGACGTGCCCTACCACGACTACTTCTACACA
ATCAATCGCTACACGCTCACCCGTGTGGCTCGGAACAAGAGCCGACTCAGGGTCTCCACA
GAGCTGCGCTATCGAAAACAGCCCTGGGGGTTAGTGAAAACGTTCATCGAGAAGAACTTC
TGGAGTGGGCTGGAGGACTACTTCCGCCATTTAGAGAGCGAGCTGGCCAAAACGGAGAGC
ACTTATTTGGCTGAGATGCACAGACAATCTCCCAAAGAGAAGGCCAGCAAGACTACAACG
GTGCGGAGGAGGAAGCGTCCCCATGCCCACCTGCGAGTCCCTCACCTGGAAGAGGTGATG
AGCCCGGTCACCACGCCCACAGATGAGGATGTGGGCCACAGGATCAAACATGTGGCAGGT
TCCACACAGACGCGGCATATCCCGGAGGACACCCCCAACGGTTTCCACCTGCAGAGCGTG
TCCAAGCTGCTGCTGGTTATCAGCTGTGTGATCTGTTTCAGTCTGGTGCTGCTGGTCATC
CTTAACATGATGCTCTTCTACAAACTCTGGATGTTGGAATACACCACGCAGACCCTCACT
GCCTGGCAGGGTCTAAGGCTCCAAGAAAGGTTACCCCAGTCTCAGACAGAATGGGCCCAG
CTCTTAGAGTCCCAACAAAAGTACCACGATACTGAGCTCCAAAAATGGAGGGAAATCATC
AAATCCTCAGTGATGCTCCTTGACCAGATGAAGGACTCGCTCATCAACCTTCAGAACGGC
ATCAGGTCCCGCGACTACACGTCGGAAAGTGAAGAAAAGAGGAATCGCTATCAT

ORF Protein

Cloned ORF protein sequence for pFN21ASDA1201 Download

>KIAA1201  698 aa
MVEKGSDHSSDKSPSTPEQGVQRSCSSQSGRSGGKNSKKSQSWYNVLSPTYKQRNEDFRK
LFKQLPDTERLIVDYSCALQRDILLQGRLYLSENWICFYSNIFRWETLLTVRLKDICSMT
KEKTARLIPNAIQVCTDSEKHFFTSFGARDRTYMMMFRLWQNALLEKPLCPKELWHFVHQ
CYGNELGLTSDDEDYVPPDDDFNTMGYCEEIPVEENEVNDSSSKSSIETKPDASPQLPKK
SITNSTLTSTGSSEAPVSFDGLPLEEEALEGDGSLEKELAIDNIMGEKIEMIAPVNSPSL
DFNDNEDIPTELSDSSDTHDEGEVQAFYEDLSGRQYVNEVFNFSVDKLYDLLFTNSPFQR
DFMEQRRFSDIIFHPWKKEENGNQSRVILYTITLTNPLAPKTATVRETQTMYKASQESEC
YVIDAEVLTHDVPYHDYFYTINRYTLTRVARNKSRLRVSTELRYRKQPWGLVKTFIEKNF
WSGLEDYFRHLESELAKTESTYLAEMHRQSPKEKASKTTTVRRRKRPHAHLRVPHLEEVM
SPVTTPTDEDVGHRIKHVAGSTQTRHIPEDTPNGFHLQSVSKLLLVISCVICFSLVLLVI
LNMMLFYKLWMLEYTTQTLTAWQGLRLQERLPQSQTEWAQLLESQQKYHDTELQKWREII
KSSVMLLDQMKDSLINLQNGIRSRDYTSESEEKRNRYH

ORF+Vector

Nucleotide Sequence (with vector) for pFN21ASDA1201 Download

>pFN21ASDA1201  6823 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCTTTCGAAGGAGATAGAACCATGGTGGAGAAGGGCTC
AGATCACTCCTCGGACAAGTCCCCGTCCACACCGGAGCAGGGCGTGCAGCGCAGCTGCTC
CTCCCAGTCCGGCCGGAGCGGCGGCAAGAATTCCAAGAAAAGCCAGAGTTGGTATAATGT
GTTAAGCCCCACCTACAAGCAGAGAAATGAAGACTTCAGAAAGCTCTTTAAGCAGCTTCC
AGACACGGAGCGCCTCATTGTTGATTACTCATGTGCACTCCAAAGAGACATTCTCCTTCA
GGGCCGACTCTACCTCTCTGAAAATTGGATCTGCTTCTACAGCAACATCTTCCGCTGGGA
AACTCTGCTGACAGTCCGTTTGAAAGACATCTGTTCCATGACTAAAGAAAAAACAGCTCG
CCTCATTCCCAATGCCATCCAAGTTTGCACTGATTCAGAAAAGCACTTCTTCACTTCGTT
TGGGGCCCGGGATAGGACATATATGATGATGTTCCGGCTCTGGCAGAATGCTCTCCTTGA
AAAGCCTCTGTGTCCCAAGGAGCTCTGGCACTTTGTTCACCAGTGCTATGGGAACGAATT
GGGCCTGACCAGTGATGACGAGGACTACGTGCCCCCTGACGACGACTTCAACACAATGGG
ATACTGTGAAGAGATCCCTGTGGAAGAGAATGAAGTGAATGACAGCTCATCCAAGAGCAG
CATAGAGACCAAGCCAGATGCCAGTCCACAGCTGCCCAAGAAATCCATCACCAACAGCAC
ACTAACATCCACAGGGAGCAGTGAGGCCCCCGTCTCGTTTGATGGGCTGCCCCTGGAGGA
AGAGGCGCTGGAAGGAGACGGGTCCCTGGAAAAGGAGCTCGCCATTGACAACATCATGGG
GGAGAAGATTGAGATGATCGCTCCTGTGAACTCCCCTTCACTGGACTTCAATGACAATGA
GGACATCCCCACTGAGCTCAGTGACTCTTCCGACACACACGATGAAGGAGAGGTCCAGGC
CTTCTATGAGGACCTGAGTGGCCGGCAGTACGTGAATGAAGTCTTCAACTTCAGCGTGGA
CAAGCTCTATGACCTCCTCTTCACCAACTCGCCCTTCCAGCGGGATTTCATGGAGCAGCG
GCGCTTCTCTGATATCATCTTCCATCCATGGAAAAAGGAGGAGAATGGAAACCAGAGCCG
AGTGATTCTTTACACCATCACCCTTACCAACCCTCTGGCTCCCAAAACTGCCACTGTCAG
GGAGACACAGACCATGTACAAGGCGAGCCAGGAGAGTGAATGTTACGTGATAGATGCCGA
AGTCCTCACCCACGACGTGCCCTACCACGACTACTTCTACACAATCAATCGCTACACGCT
CACCCGTGTGGCTCGGAACAAGAGCCGACTCAGGGTCTCCACAGAGCTGCGCTATCGAAA
ACAGCCCTGGGGGTTAGTGAAAACGTTCATCGAGAAGAACTTCTGGAGTGGGCTGGAGGA
CTACTTCCGCCATTTAGAGAGCGAGCTGGCCAAAACGGAGAGCACTTATTTGGCTGAGAT
GCACAGACAATCTCCCAAAGAGAAGGCCAGCAAGACTACAACGGTGCGGAGGAGGAAGCG
TCCCCATGCCCACCTGCGAGTCCCTCACCTGGAAGAGGTGATGAGCCCGGTCACCACGCC
CACAGATGAGGATGTGGGCCACAGGATCAAACATGTGGCAGGTTCCACACAGACGCGGCA
TATCCCGGAGGACACCCCCAACGGTTTCCACCTGCAGAGCGTGTCCAAGCTGCTGCTGGT
TATCAGCTGTGTGATCTGTTTCAGTCTGGTGCTGCTGGTCATCCTTAACATGATGCTCTT
CTACAAACTCTGGATGTTGGAATACACCACGCAGACCCTCACTGCCTGGCAGGGTCTAAG
GCTCCAAGAAAGGTTACCCCAGTCTCAGACAGAATGGGCCCAGCTCTTAGAGTCCCAACA
AAAGTACCACGATACTGAGCTCCAAAAATGGAGGGAAATCATCAAATCCTCAGTGATGCT
CCTTGACCAGATGAAGGACTCGCTCATCAACCTTCAGAACGGCATCAGGTCCCGCGACTA
CACGTCGGAAAGTGAAGAAAAGAGGAATCGCTATCATTACGTAGTTTAAACGAATTCGGG
CTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTA
ACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAAC
CCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACC
ACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTA
TTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATG
TTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGT
GGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCC
CAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATG
GTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTA
TTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTT
CAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCC
TTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAA
GATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGT
AAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTT
CTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGC
ATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACG
GATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCG
GCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAAC
ATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCA
AACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTA
ACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGAT
AAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAA
TCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAG
CCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAAT
AGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGAC
CAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAAT
CAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTA
AAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAA
ATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTC
CCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGT
CCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCA
GTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCG
ACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTAT
CGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTA
CAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCT
GCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAAC
AAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAA
AAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAA
ACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTT
TATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCAT
GAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCA
TAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTG
TTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACA
ATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGC
AGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT