HUGE

Gene/Protein Characteristic Table for KIAA1972

Link to : Rouge \| GTOP \| SWISS-PROT/TrEMBL \| GeneCards\| RefDIC
Features : DNA sequence \| Protein sequence \| Expression \| Mapping

Product ID :	ORK00963
Accession No. :	AB075852
Description :	RING finger and SPRY domain-containing protein 1 precursor.
HUGO Gene Name :	ring finger and SPRY domain containing 1 (RSPRY1)
Clone Name :	fk09334 [Vector Info]
Flexi ORF Clone :	pF1KSDA1972
Source :	Human fetal brain

Features of the cloned DNA sequence	Description

Length: 3473 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis) for :
cloned DNA seq.
Warning for N-terminal truncation:
NO

Warning for coding interruption:
NO

Integrity of 3' end

Length of 3'UTR 1499 bp

Genome contig ID gi51511732f_55677755
PolyA signal sequence
(AATAAA,-31)
+----*----+----*----+----*----+----
GAAGAATAAAACATCTGTTGCCTTTTTTGACTAAG
Flanking genome sequence
(154134 - 154183)
----+----*----+----*----+----*----+----*----+----*
ATTTCACAACTTTCATTGGTGTATACTGCTAAGGTTCCAAGACATTATAC

Ensembl ContigView (Add our DAS server as a DAS source)

Chr f/r start end exon identity class

ContigView(URL based/DAS) 16 f 55777755 55831887 15 99.5 Perfect prediction

Features of the protein sequence	Description

Length: 576 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
Q5R881	99.8	RING finger and...	Pongo abelii
BAG51341	99.8	unnamed protein...	Homo sapiens
Q95LP3	99.1	RING finger and...	Macaca fascicularis
XP_850920	97.2	similar to F16A...	Canis lupus fam...
AAI34576	96.9	RSPRY1 protein ...	Bos taurus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other HUGE database (FASTA output)
No significant homologues
Result of motif / domain search (InterProScan and SOSUI)

[ zoom out (x2) ]

Result of InterProScan

Search method interpro_ID From To Entry Definition

HMMPfam IPR003877 358 482 PF00622 SPla/RYanodine receptor SPRY

HMMSmart IPR003877 358 482 SM00449 SPla/RYanodine receptor SPRY

IPR001841 527 561 SM00184 Zinc finger

ProfileScan IPR001870 300 483 PS50188 B302

IPR001841 527 562 PS50089 Zinc finger

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR003877	358	482	PF00622	SPla/RYanodine receptor SPRY
HMMSmart	IPR003877	358	482	SM00449	SPla/RYanodine receptor SPRY
IPR001841	527	561	SM00184	Zinc finger
ProfileScan	IPR001870	300	483	PS50188	B302
IPR001841	527	562	PS50089	Zinc finger

Prediction of transmembrane (TM) segments

Method No. N terminal transmembrane region C terminal type length

SOSUI2 1 1 MIVFGWAVFLASRSLGQGLLLTL 23 PRIMARY 23

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	1	MIVFGWAVFLASRSLGQGLLLTL	23	PRIMARY	23

Expression profile	Description

: RT-PCR-ELISA Description
: Experimental conditions

Primer_f
: CCAGTATTAGTGACCGGCTTG

Primer_r
: GGTACTCGCTGACATCATTGC

PCR conditions
: 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RT-PCR-ELISA	Description

RH mapping information	Description

Chromosome No.

: 16

Experimental conditions

Panel name	: genbank
Primer_f	: -
Primer_r	: -
PCR product length	: -
PCR conditions	: -

How to obtain KIAA clone(s) Back to the HUGE Protein Database homepage

	cloned DNA seq.
Warning for N-terminal truncation:	NO
Warning for coding interruption:	NO

Length of 3'UTR	1499 bp
Genome contig ID	gi51511732f_55677755
PolyA signal sequence (AATAAA,-31)	+--------+--------+----*----+---- GAAGAATAAAACATCTGTTGCCTTTTTTGACTAAG
Flanking genome sequence (154134 - 154183)	----+--------+--------+--------+--------+----* ATTTCACAACTTTCATTGGTGTATACTGCTAAGGTTCCAAGACATTATAC

	Chr	f/r	start	end	exon	identity	class
ContigView(URL based/DAS)	16	f	55777755	55831887	15	99.5	Perfect prediction

Primer_f	: CCAGTATTAGTGACCGGCTTG
Primer_r	: GGTACTCGCTGACATCATTGC
PCR conditions	: 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Gene/Protein Characteristic Table for KIAA1972

Features of the cloned DNA sequence

Features of the protein sequence

Expression profile

RH mapping information