HUGE

Gene/Protein Characteristic Table for KIAA1810

Link to : Rouge \| GTOP \| SWISS-PROT/TrEMBL \| GeneCards\| RefDIC
Features : DNA sequence \| Protein sequence \| Expression \| Mapping

Product ID :	ORK00924
Accession No. :	AB058713
Description :	E3 ubiquitin-protein ligase synoviolin precursor.
HUGO Gene Name :
Clone Name :	fk03376 [Vector Info]
Flexi ORF Clone :	pF1KSDA1810
Source :	Human fetal brain

Features of the cloned DNA sequence	Description

Length: 2841 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis) for :
cloned DNA seq.
Warning for N-terminal truncation:
YES

Warning for coding interruption:
NO

Integrity of 3' end

Length of 3'UTR 1101 bp

Genome contig ID gi51511727r_64551329
PolyA signal sequence
(AATAAA,-19)
+----*----+----*----+----*----+----
ATTGTATATACAGCCAAATAAAAACTTGCATTAAC
Flanking genome sequence
(100000 - 99951)
----+----*----+----*----+----*----+----*----+----*
AATTGGTGGGGTTTGTGTCACAGAATCGATCAAATCAGTGTCGGGCTCCT

Ensembl ContigView (Add our DAS server as a DAS source)

Chr f/r start end exon identity class

ContigView(URL based/DAS) 11 r 64651329 64658527 14 99.6 Perfect prediction

Features of the protein sequence	Description

Length: 579 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
EAW74361	5.2e-143	100.0	synovial apopto...	Homo sapiens
AAL26903	8.4e-111	91.7	HRD1 [Homo sapi...	Homo sapiens
CAD38937	8.4e-111	91.7	hypothetical pr...	Homo sapiens
Q86TM6	2.5e-110	91.6	E3 ubiquitin-pr...	Homo sapiens
ABZ92145	3.2e-110	91.4	synovial apopto...	synthetic construct

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other HUGE database (FASTA output)
No significant homologues
Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method interpro_ID From To Entry Definition

FPrintScan NULL 302 323 PR01217 NULL

NULL 323 339 PR01217 NULL

NULL 348 365 PR01217 NULL

NULL 419 444 PR01217 NULL

HMMPfam IPR001841 254 292 PF00097 Zinc finger

HMMSmart IPR001841 254 292 SM00184 Zinc finger

ProfileScan IPR001841 254 293 PS50089 Zinc finger

Search method	interpro_ID	From	To	Entry	Definition
FPrintScan	NULL	302	323	PR01217	NULL
NULL	323	339	PR01217	NULL
NULL	348	365	PR01217	NULL
NULL	419	444	PR01217	NULL
HMMPfam	IPR001841	254	292	PF00097	Zinc finger
HMMSmart	IPR001841	254	292	SM00184	Zinc finger
ProfileScan	IPR001841	254	293	PS50089	Zinc finger

Prediction of transmembrane (TM) segments

Method No. N terminal transmembrane region C terminal type length

SOSUI2 1 16 FRTAVMMAASLALTGAVVAHAYY 38 PRIMARY 23

2 54 SPSMAVLYIQAFVLVFLLGKVMG 76 PRIMARY 23

3 139 DFYAILMTMVLTIFIKYVLHSVD 161 SECONDARY 23

4 182 TGFIKVLLYMAFMTIMIKVHTFP 204 PRIMARY 23

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	16	FRTAVMMAASLALTGAVVAHAYY	38	PRIMARY	23
2	54	SPSMAVLYIQAFVLVFLLGKVMG	76	PRIMARY	23
3	139	DFYAILMTMVLTIFIKYVLHSVD	161	SECONDARY	23
4	182	TGFIKVLLYMAFMTIMIKVHTFP	204	PRIMARY	23

Expression profile	Description

: RT-PCR-ELISA Description
: Experimental conditions

Primer_f
: CCTTCCTCTCCAACTCTTCAG

Primer_r
: TGGGAACGGGAGCTAATACTG

PCR conditions
: 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RT-PCR-ELISA	Description

RH mapping information	Description

Chromosome No.

: 11

Experimental conditions

Panel name	: unigene
Primer_f	: -
Primer_r	: -
PCR product length	: -
PCR conditions	: -

How to obtain KIAA clone(s) Back to the HUGE Protein Database homepage

	cloned DNA seq.
Warning for N-terminal truncation:	YES
Warning for coding interruption:	NO

Length of 3'UTR	1101 bp
Genome contig ID	gi51511727r_64551329
PolyA signal sequence (AATAAA,-19)	+--------+--------+----*----+---- ATTGTATATACAGCCAAATAAAAACTTGCATTAAC
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* AATTGGTGGGGTTTGTGTCACAGAATCGATCAAATCAGTGTCGGGCTCCT

	Chr	f/r	start	end	exon	identity	class
ContigView(URL based/DAS)	11	r	64651329	64658527	14	99.6	Perfect prediction

Primer_f	: CCTTCCTCTCCAACTCTTCAG
Primer_r	: TGGGAACGGGAGCTAATACTG
PCR conditions	: 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Gene/Protein Characteristic Table for KIAA1810

Features of the cloned DNA sequence

Features of the protein sequence

Expression profile

RH mapping information