HUGE

Gene/Protein Characteristic Table for KIAA1635

Link to : Rouge \| GTOP \| SWISS-PROT/TrEMBL \| GeneCards\| RefDIC
Features : DNA sequence \| Protein sequence \| Expression \| Mapping

Product ID :
Accession No. :	AB046855
Description :	mediator of RNA polymerase II transcription, subunit 12 homolog (S. cerevisiae)-like.
HUGO Gene Name :	mediator complex subunit 12-like (MED12L)
Clone Name :	fh23184 [Vector Info]
Source :	Human fetal brain

Features of the cloned DNA sequence	Description

Length: 5808 bp
Physical map
Restriction map
The cloned DNA sequence was revised by direct RT-PCR/sequencing experiments following the alert of coding interruption by GeneMark analysis.
Comparison between the cloned and the revised DNA sequences.
Prediction of protein coding region (GeneMark analysis) for :
cloned DNA seq. revised DNA seq.
Warning for N-terminal truncation:
YES YES

Warning for coding interruption:
YES NO

Integrity of 3' end

Length of 3'UTR 1232 bp

Genome contig ID gi89161205f_152450536
PolyA signal sequence
(None)
+----*----+----*----+----*----+----
CAGTTTGTGTAACTTAAGAGATCGCGAGCGGCCGC
Flanking genome sequence None

Ensembl ContigView (Add our DAS server as a DAS source)

Chr f/r start end exon identity class

ContigView(URL based/DAS) 3 f 152550533 152634501 29 99.7 Terminal No-hit

Features of the protein sequence	Description

Length: 1435 aa
This protein sequence is predicted from the revised DNA sequence

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
Q86YW9	100.0	Mediator of RNA...	Homo sapiens
AAO38813	100.0	TRALPUSH [Homo ...	Homo sapiens
XP_516821	99.8	mediator of RNA...	Pan troglodytes
EAW78801	99.6	mediator of RNA...	Homo sapiens
XP_001107022	99.7	similar to medi...	Macaca mulatta

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
D83783	4.6e-57	58.5	KIAA0192

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

Motif_DB interpro_ID From To Entry Definition

None - - - - -

Motif_DB	interpro_ID	From	To	Entry	Definition
None	-	-	-	-	-

Prediction of transmembrane (TM) segments

Method No. N terminal transmembrane region C terminal type length

- - - - - - -

Method	No.	N terminal	transmembrane region	C terminal	type	length
-	-	-	-	-	-	-

Expression profile	Description

: RT-PCR-ELISA Description
: Experimental conditions

Primer_f
: AACAGCATTGAAGTGGGAGCC

Primer_r
: TTTGGATTTTGTGAGGCAGTC

PCR conditions
: 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RT-PCR-ELISA	Description

RH mapping information	Description

Chromosome No.

: 3

Experimental conditions

Panel name	: CCR
Primer_f	: GGCAATCCTGGTTAGAACTCC
Primer_r	: TACTGTTTGGGTTGAAGAGGC
PCR product length	: 186(300) bp
PCR conditions	: 95 °C15 sec66 °C60 sec30 cycles

How to obtain KIAA clone(s) Back to the HUGE Protein Database homepage

	cloned DNA seq.	revised DNA seq.
Warning for N-terminal truncation:	YES	YES
Warning for coding interruption:	YES	NO

Length of 3'UTR	1232 bp
Genome contig ID	gi89161205f_152450536
PolyA signal sequence (None)	+--------+--------+----*----+---- CAGTTTGTGTAACTTAAGAGATCGCGAGCGGCCGC
Flanking genome sequence	None

	Chr	f/r	start	end	exon	identity	class
ContigView(URL based/DAS)	3	f	152550533	152634501	29	99.7	Terminal No-hit

Primer_f	: AACAGCATTGAAGTGGGAGCC
Primer_r	: TTTGGATTTTGTGAGGCAGTC
PCR conditions	: 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Gene/Protein Characteristic Table for KIAA1635

Features of the cloned DNA sequence

Features of the protein sequence

Expression profile

RH mapping information