##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
Acc65I AccI AccIII AgeI Alw44I
Aor51HI ApaLI AscI Asp700I Asp718I
AspI AvaI AvrII BalI BlnI
BsaWI BseAI BsiWI BsmBI BsoBI
BspDI BspEI BsrFI BssHII Bst1107I
BstEII BstPI BstXI BstZ17I Cfr10I
ClaI CpoI DrdI Eco47III EcoO65I
FseI KpnI KspI MfeI MluI
MluNI MroI MscI MunI NaeI
NcoI NdeI NgoMIV PacI PaeR7I
PflFI PinAI PshAI PvuI RsrII
SacII SalI SexAI SfiI SgrAI
SmaI SnaBI SpeI SphI SwaI
Tth111I XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AatII 1173 GACGTC GACGT^C
AclI 2871 AACGTT AA^CGTT
ApaI 2765 GGGCCC GGGCC^C
AseI 4932 ATTAAT AT^TAAT
AsnI 4932 ATTAAT AT^TAAT
AviII 3885 TGCGCA TGC^GCA
BanI 3724 GGCGCC G^G[CT][GA]CC
BbeI 3724 GGCGCC GGCGC^C
BbrPI 1056 CACGTG CAC^GTG
BciVI 3918 GTATCC GTATCC(6/5)
BglI 4048 GCCTATCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 5014 AGATCT A^GATCT
BlpI 3391 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3391 GCTGAGC GC^T[ACGT]AGC
BsaAI 1056 CACGTG [CT]AC^GT[GA]
Bsp1407I 1674 TGTACA T^GTACA
BsrBI 5799 GAGCGG CCGCTC(-3/-3)
BsrGI 1674 TGTACA T^GTACA
BstBI 779 TTCGAA TT^CGAA
CelII 3391 GCTGAGC GC^T[ACGT]AGC
DsaI 3154 CCGTGG C^C[GA][CT]GG
EcoNI 4266 CCTGCCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 4771 GAATTC G^AATTC
FspI 3885 TGCGCA TGC^GCA
HpaI 2349 GTTAAC GTT^AAC
KasI 3724 GGCGCC G^GCGCC
NarI 3724 GGCGCC GG^CGCC
NheI 4837 GCTAGC G^CTAGC
NotI 5801 GCGGCCGC GC^GGCCGC
NruI 5795 TCGCGA TCG^CGA
NspV 779 TTCGAA TT^CGAA
PmaCI 1056 CACGTG CAC^GTG
PmeI 4728 GTTTAAAC GTTT^AAAC
PmlI 1056 CACGTG CAC^GTG
PpuMI 1888 AGGACCC [GA]G^G[AT]CC[CT]
PshBI 4932 ATTAAT AT^TAAT
Psp1406I 2871 AACGTT AA^CGTT
PvuII 99 CAGCTG CAG^CTG
SacI 1282 GAGCTC GAGCT^C
SbfI 3658 CCTGCAGG CCTGCA^GG
ScaI 5202 AGTACT AGT^ACT
SfoI 3724 GGCGCC GGC^GCC
SfuI 779 TTCGAA TT^CGAA
Sse8387I 3658 CCTGCAGG CCTGCA^GG
SspBI 1674 TGTACA T^GTACA
SspI 1935 AATATT AAT^ATT
XbaI 4882 TCTAGA T^CTAGA
XcmI 935 CCATCAGGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccII 2673 CGCG CG^CG
5796 CGCG
AocI 3331 CCTGAGG CC^T[ACGT]AGG
4270 CCTCAGG
BamHI 794 GGATCC G^GATCC
4275 GGATCC
BbsI 2476 GAAGAC GAAGAC(2/6)
4590 GAAGAC
BbvCI 829 CCTCAGC CCTCAGC(-5/-2)
3904 CCTCAGC
BcgI 3754 GCAATCACATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
5374 GCAGTGGAATCG
BpuAI 2476 GAAGAC GAAGAC(2/6)
4590 GAAGAC
BsaBI 261 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3481 GATGAATATC
BsmI 1511 GAATGC GAATGC(1/-1)
5046 GCATTC
BspLU11I 3028 ACATGT A^CATGT
5344 ACATGT
BspMI 2289 ACCTGC ACCTGC(4/8)
4145 ACCTGC
BssSI 2051 CACGAG CACGAG(-5/-1)
3583 CTCGTG
BstAPI 2677 GCAGATGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4450 GCAAGGCTTGC
BstUI 2673 CGCG CG^CG
5796 CGCG
Bsu36I 3331 CCTGAGG CC^T[ACGT]AGG
4270 CCTCAGG
DraII 1888 AGGACCC [GA]G^G[ACGT]CC[CT]
2765 GGGCCCT
DraIII 2745 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
3564 CACCTTGTG
Eco81I 3331 CCTGAGG CC^T[ACGT]AGG
4270 CCTCAGG
EcoO109I 1888 AGGACCC [GA]G^G[ACGT]CC[CT]
2765 GGGCCCT
EcoRV 120 GATATC GAT^ATC
1521 GATATC
HgaI 1230 GACGC GACGC(5/10)
321 GCGTC
HindIII 314 AAGCTT A^AGCTT
3787 AAGCTT
MamI 261 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3481 GATGAATATC
MvnI 2673 CGCG CG^CG
5796 CGCG
PflMI 3563 CCACCTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5451 CCAGTAGCTGG
Van91I 3563 CCACCTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5451 CCAGTAGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AciI AcsI AcyI AfaI AflII
AflIII AhdI AluI AlwI AlwNI
ApoI AspEI AspHI AvaII BanII
BbvI BclI BcnI BfaI BfrI
BmyI BpmI BsaHI BsaI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspHI
BsrDI BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI EaeI EagI Eam1105I
EarI EclXI Eco52I EcoRII EcoT14I
EcoT22I FauI FbaI Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NsiI NspI PleI PstI RcaI
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII