HUGE

Gene/Protein Characteristic Table for KIAA1423

Link to : Rouge \| GTOP \| SWISS-PROT/TrEMBL \| GeneCards\| RefDIC
Features : DNA sequence \| Protein sequence \| Expression \| Mapping

Product ID :
Accession No. :	AB037844
Description :	Transmembrane protein 181.
HUGO Gene Name :
Clone Name :	fh08369 [Vector Info]
Source :	Human fetal brain

Features of the cloned DNA sequence	Description

Length: 5286 bp
Physical map
Restriction map
The cloned DNA sequence was revised by direct RT-PCR/sequencing experiments following the alert of coding interruption by GeneMark analysis.
Comparison between the cloned and the revised DNA sequences.
Prediction of protein coding region (GeneMark analysis) for :
cloned DNA seq. revised DNA seq.
Warning for N-terminal truncation:
NO YES

Warning for coding interruption:
YES NO

Integrity of 3' end

Length of 3'UTR 3538 bp

Genome contig ID gi89161210f_158777456
PolyA signal sequence
(AATAAA,-18)
+----*----+----*----+----*----+----
CCTTTCTGGGAAATGTTAATAAATTTTTAATATTC
Flanking genome sequence
(198994 - 199043)
----+----*----+----*----+----*----+----*----+----*
ACTTCTATGTGTCTTGATTTGTTTGCTCTTTGTGTGTGGAGTTAATTTGG

Ensembl ContigView (Add our DAS server as a DAS source)

Chr f/r start end exon identity class

ContigView(URL based/DAS) 6 f 158877454 158976448 17 99.2 Perfect prediction

Features of the protein sequence	Description

Length: 616 aa
This protein sequence is predicted from the revised DNA sequence

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
Q9P2C4	3.2e-197	100.0	Transmembrane p...	Homo sapiens
EAW47660	1.3e-196	99.7	hCG18157 [Homo ...	Homo sapiens
XP_001914707	4e-155	94.2	transmembrane p...	Equus caballus
XP_001381390	1.7e-142	86.0	similar to G pr...	Monodelphis dom...
XP_870842	6.4e-139	93.6	similar to Tran...	Bos taurus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other HUGE database (FASTA output)
No significant homologues
Result of motif / domain search (InterProScan and SOSUI)

[ zoom out (x2) ]

Result of InterProScan

Search method interpro_ID From To Entry Definition

None - - - - -

Search method	interpro_ID	From	To	Entry	Definition
None	-	-	-	-	-

Prediction of transmembrane (TM) segments

Method No. N terminal transmembrane region C terminal type length

SOSUI2 1 157 HFVLVFVVFFICFGLTIFVGIRG 179 PRIMARY 23

2 316 LEIWFRFFFVVLTFIVTCLFAHS 338 PRIMARY 23

3 353 WMSVLLPLLLLYNDPFFPLSFLV 375 SECONDARY 23

4 387 FQSMFLCALLLFWLCVYHGIRVQ 409 PRIMARY 23

5 416 TFYLPKFFIVGLLWLASVTLGIW 438 PRIMARY 23

6 462 KVFFMVVAAVYILYLLFLIVRAC 484 PRIMARY 23

7 498 KFLTALTFVVLVISIAILYLRFG 520 PRIMARY 23

8 541 EFLSFYGLLNFYLYTLAFVYSPS 563 SECONDARY 23

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	157	HFVLVFVVFFICFGLTIFVGIRG	179	PRIMARY	23
2	316	LEIWFRFFFVVLTFIVTCLFAHS	338	PRIMARY	23
3	353	WMSVLLPLLLLYNDPFFPLSFLV	375	SECONDARY	23
4	387	FQSMFLCALLLFWLCVYHGIRVQ	409	PRIMARY	23
5	416	TFYLPKFFIVGLLWLASVTLGIW	438	PRIMARY	23
6	462	KVFFMVVAAVYILYLLFLIVRAC	484	PRIMARY	23
7	498	KFLTALTFVVLVISIAILYLRFG	520	PRIMARY	23
8	541	EFLSFYGLLNFYLYTLAFVYSPS	563	SECONDARY	23

Expression profile	Description

: RT-PCR-ELISA Description
: Experimental conditions

Primer_f
: TCAGCCGAGTTCTTATCTTTC

Primer_r
: AATCACATCATCATCCGAGTC

PCR conditions
: 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RT-PCR-ELISA	Description

RH mapping information	Description

Chromosome No.

: 6

Experimental conditions

Panel name	: unigene
Primer_f	: -
Primer_r	: -
PCR product length	: -
PCR conditions	: -

How to obtain KIAA clone(s) Back to the HUGE Protein Database homepage

	cloned DNA seq.	revised DNA seq.
Warning for N-terminal truncation:	NO	YES
Warning for coding interruption:	YES	NO

Length of 3'UTR	3538 bp
Genome contig ID	gi89161210f_158777456
PolyA signal sequence (AATAAA,-18)	+--------+--------+----*----+---- CCTTTCTGGGAAATGTTAATAAATTTTTAATATTC
Flanking genome sequence (198994 - 199043)	----+--------+--------+--------+--------+----* ACTTCTATGTGTCTTGATTTGTTTGCTCTTTGTGTGTGGAGTTAATTTGG

	Chr	f/r	start	end	exon	identity	class
ContigView(URL based/DAS)	6	f	158877454	158976448	17	99.2	Perfect prediction

Primer_f	: TCAGCCGAGTTCTTATCTTTC
Primer_r	: AATCACATCATCATCCGAGTC
PCR conditions	: 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Gene/Protein Characteristic Table for KIAA1423

Features of the cloned DNA sequence

Features of the protein sequence

Expression profile

RH mapping information