##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
Acc65I AclI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI BamHI BbeI
BbrPI BciVI BclI BlpI Bpu1102I
BsaAI BsiEI BsiWI BspDI BstBI
Bsu36I CelII ClaI CpoI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoRV FbaI FseI
HaeII KasI KpnI MluI NarI
NdeI NheI NotI NruI NspV
PacI PaeR7I PflMI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RsrII
SalI SbfI SfoI SfuI SgrAI
SnaBI SpeI Sse8387I Van91I XbaI
XcmI XhoI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AatII 3245 GACGTC GACGT^C
AgeI 4856 ACCGGT A^CCGGT
AlwNI 2572 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG
AvaI 284 CCCGGG C^[CT]CG[GA]G
AviII 902 TGCGCA TGC^GCA
AvrII 4404 CCTAGG C^CTAGG
BanI 4479 GGTGCC G^G[CT][GA]CC
BcgI 310 CGAGTACCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 4905 AGATCT A^GATCT
BlnI 4404 CCTAGG C^CTAGG
BsaBI 4900 GATACAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsoBI 284 CCCGGG C^[CT]CG[GA]G
BspHI 3480 TCATGA T^CATGA
BsrDI 3656 GCAATG GCAATG(2/0)
BssSI 3078 CTCGTG CACGAG(-5/-1)
Bst1107I 752 GTATAC GTA^TAC
BstZ17I 752 GTATAC GTA^TAC
EcoNI 2522 CCTGTGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1508 GAATTC G^AATTC
FspI 902 TGCGCA TGC^GCA
HgaI 305 GACGC GACGC(5/10)
KspI 2 CCGCGG CCGC^GG
MamI 4900 GATACAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3334 CAATTG C^AATTG
MunI 3334 CAATTG C^AATTG
NaeI 360 GCCGGC GCC^GGC
NgoMIV 360 GCCGGC G^CCGGC
PinAI 4856 ACCGGT A^CCGGT
PshAI 4640 GACAAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 3480 TCATGA T^CATGA
SacII 2 CCGCGG CCGC^GG
SapI 1085 GCTCTTC GCTCTTC(1/4)
SexAI 2255 ACCAGGT A^CC[AT]GGT
SfiI 1706 GGCCATCCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 284 CCCGGG CCC^GGG
StuI 39 AGGCCT AGG^CCT
SwaI 1907 ATTTAAAT ATTT^AAAT
XmaCI 284 CCCGGG C^CCGGG
XmaI 284 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AccI 521 GTCTAC GT^[AC][GT]AC
752 GTATAC
AccIII 913 TCCGGA T^CCGGA
3086 TCCGGA
AcyI 305 GACGCC G[GA]^CG[CT]C
3245 GACGTC
AflII 4032 CTTAAG C^TTAAG
4496 CTTAAG
AflIII 549 ACATGT A^C[GA][CT]GT
690 ACATGT
Asp700I 1280 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3090 GAAGGGGTTC
AspI 2111 GACTAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2751 GACCCCGTC
BbvCI 2119 CCTCAGC CCTCAGC(-5/-2)
2827 GCTGAGG
BfrI 4032 CTTAAG C^TTAAG
4496 CTTAAG
BsaHI 305 GACGCC G[GA]^CG[CT]C
3245 GACGTC
BsaI 2726 GAGACC GGTCTC(1/5)
2749 GAGACC
BseAI 913 TCCGGA T^CCGGA
3086 TCCGGA
BsmBI 2756 CGTCTC CGTCTC(1/5)
3247 CGTCTC
BspEI 913 TCCGGA T^CCGGA
3086 TCCGGA
BspLU11I 549 ACATGT A^CATGT
690 ACATGT
BspMI 172 ACCTGC ACCTGC(4/8)
4629 GCAGGT
BsrBI 332 CCGCTC CCGCTC(-3/-3)
267 GAGCGG
BsrFI 360 GCCGGC [GA]^CCGG[CT]
4856 ACCGGT
BssHII 146 GCGCGC G^CGCGC
200 GCGCGC
BstXI 2240 CCAGTGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3811 CCATGATTTTGG
BstYI 1376 GGATCT [GA]^GATC[CT]
4905 AGATCT
Cfr10I 360 GCCGGC [GA]^CCGG[CT]
4856 ACCGGT
EcoT22I 3397 ATGCAT ATGCA^T
3953 ATGCAT
Hin1I 305 GACGCC G[GA]^CG[CT]C
3245 GACGTC
HindIII 596 AAGCTT A^AGCTT
4590 AAGCTT
HpaI 1218 GTTAAC GTT^AAC
3289 GTTAAC
MflI 1376 GGATCT [GA]^GATC[CT]
4905 AGATCT
MroI 913 TCCGGA T^CCGGA
3086 TCCGGA
NcoI 2156 CCATGG C^CATGG
2471 CCATGG
NsiI 3397 ATGCAT ATGCA^T
3953 ATGCAT
PflFI 2111 GACTAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2751 GACCCCGTC
PvuII 1025 CAGCTG CAG^CTG
1608 CAGCTG
SacI 344 GAGCTC GAGCT^C
386 GAGCTC
SphI 2788 GCATGC GCATG^C
2798 GCATGC
Tth111I 2111 GACTAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2751 GACCCCGTC
XhoII 1376 GGATCT [GA]^GATC[CT]
4905 AGATCT
XmnI 1280 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3090 GAAGGGGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI AspHI AvaII BalI
BanII BbsI BbvI BcnI BfaI
BglI BmyI BpmI BpuAI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
Bsp1407I BsrGI BsrI BssKI BstAPI
BstEII BstF5I BstNI BstPI BstUI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
EcoO109I EcoO65I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PpuMI
PstI RsaI Sau3AI Sau96I ScaI
ScrFI SfaNI SfcI SmlI SspBI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I