HUGE

Gene/Protein Characteristic Table for KIAA1083

Link to : Rouge \| GTOP \| SWISS-PROT/TrEMBL \| GeneCards\| RefDIC
Features : DNA sequence \| Protein sequence \| Expression \| Mapping

Product ID :	ORK00733
Accession No. :	AB029006
Description :	Spastin.
HUGO Gene Name :	spastin (SPAST)
Clone Name :	hj07423 [Vector Info]
Flexi ORF Clone :	pF1KSDA1083
Source :	Human adult brain

Features of the cloned DNA sequence	Description

Length: 5120 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis) for :
cloned DNA seq.
Warning for N-terminal truncation:
NO

Warning for coding interruption:
NO

Integrity of 3' end

Length of 3'UTR 3144 bp

Genome contig ID gi89161199f_32042184
PolyA signal sequence
(AATAAA,-17)
+----*----+----*----+----*----+----
TTATCCTAATGTATTGGAAATAAATGATAAACTTT
Flanking genome sequence
(194027 - 194076)
----+----*----+----*----+----*----+----*----+----*
ATTTTGTCTTTACTTTTTTACTTTAAAACTTTTTGATATTTTAGGGGTTG

Ensembl ContigView (Add our DAS server as a DAS source)

Chr f/r start end exon identity class

ContigView(URL based/DAS) 2 f 32142184 32236209 16 99.4 Perfect prediction

Features of the protein sequence	Description

Length: 584 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
XP_862831	1.6e-175	94.9	similar to spas...	Canis lupus fam...
AAI66846	2.7e-169	93.8	Spast protein [...	Rattus norvegicus
XP_001371530	4.4e-150	90.3	similar to spas...	Monodelphis dom...
ACF60961	1.7e-131	87.9	neuronal spasti...	Gallus gallus
Q9UBP0	8.8e-118	94.8	Spastin.	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other HUGE database (FASTA output)
No significant homologues
Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method interpro_ID From To Entry Definition

HMMPfam IPR003959 345 530 PF00004 AAA ATPase

HMMSmart IPR007330 116 194 SM00745 MIT

IPR003593 342 478 SM00382 AAA+ ATPase

ScanRegExp IPR003960 448 467 PS00674 AAA ATPase

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR003959	345	530	PF00004	AAA ATPase
HMMSmart	IPR007330	116	194	SM00745	MIT
IPR003593	342	478	SM00382	AAA+ ATPase
ScanRegExp	IPR003960	448	467	PS00674	AAA ATPase

Prediction of transmembrane (TM) segments

Method No. N terminal transmembrane region C terminal type length

SOSUI2 1 55 YPLFVGFALLRLVAFHLGLLFVW 77 PRIMARY 23

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	55	YPLFVGFALLRLVAFHLGLLFVW	77	PRIMARY	23

Expression profile	Description

: RT-PCR-ELISA Description
: Experimental conditions

Primer_f
: TCGGTGTTGAGCTCTGTGTTG

Primer_r
: ACTCATTCTTTCCCTTGGCTC

PCR conditions
: 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RT-PCR-ELISA	Description

RH mapping information	Description

Chromosome No.

: 2

Experimental conditions

Panel name	: GeneBridge 4
Primer_f	: TCGGTGTTGAGCTCTGTGTTG
Primer_r	: ACTCATTCTTTCCCTTGGCTC
PCR product length	: 151 bp
PCR conditions	: 95 °C15 sec64 °C60 sec35 cycles

How to obtain KIAA clone(s) Back to the HUGE Protein Database homepage

	cloned DNA seq.
Warning for N-terminal truncation:	NO
Warning for coding interruption:	NO

Length of 3'UTR	3144 bp
Genome contig ID	gi89161199f_32042184
PolyA signal sequence (AATAAA,-17)	+--------+--------+----*----+---- TTATCCTAATGTATTGGAAATAAATGATAAACTTT
Flanking genome sequence (194027 - 194076)	----+--------+--------+--------+--------+----* ATTTTGTCTTTACTTTTTTACTTTAAAACTTTTTGATATTTTAGGGGTTG

	Chr	f/r	start	end	exon	identity	class
ContigView(URL based/DAS)	2	f	32142184	32236209	16	99.4	Perfect prediction

Gene/Protein Characteristic Table for KIAA1083

Features of the cloned DNA sequence

Features of the protein sequence

Expression profile

RH mapping information