HUGE

Gene/Protein Characteristic Table for KIAA0761

Link to : GTOP \| SWISS-PROT/TrEMBL \| GeneCards\| RefDIC
Features : DNA sequence \| Protein sequence \| Expression \| Mapping

Product ID :	ORK00125
Accession No. :	AB018304
Description :	Mid-1-related chloride channel 1 isoform 1.
HUGO Gene Name :
Clone Name :	hk04655s1 [Vector Info]
Flexi ORF Clone :	pF1KA0761
Source :	Human adult brain
Note :	We replaced hk04655, former representative clones for KIAA0761 with hk04655s1. (2002/12/27)

Features of the cloned DNA sequence	Description

Length: 4696 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis) for :
cloned DNA seq.
Warning for N-terminal truncation:
YES

Warning for coding interruption:
NO

Integrity of 3' end

Length of 3'UTR 3039 bp

Genome contig ID gi89161185r_109173653
PolyA signal sequence
(AAGAAA,-21)
+----*----+----*----+----*----+----
AGCCTGATTTCTGAAAGAAATAATCTTTAAACCTG
Flanking genome sequence
(100000 - 99951)
----+----*----+----*----+----*----+----*----+----*
AAACTGCATAAAATTTTTCCTTTAAAATGGCTTATATAGGTATAGCTGCT

Ensembl ContigView (Add our DAS server as a DAS source)

Chr f/r start end exon identity class

ContigView(URL based/DAS) 1 r 109273653 109294583 11 99.3 Perfect prediction

Features of the protein sequence	Description

Length: 551 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
XP_513612	0	99.3	Mid-1-related c...	Pan troglodytes
XP_001087523	3.4e-212	96.4	similar to Mid-...	Macaca mulatta
Q1LZF8	3.4e-177	82.0	Chloride channe...	Bos taurus
XP_001927252	3.5e-173	79.5	chloride channe...	Sus scrofa
AAH81736	6.2e-156	73.2	Clcc1 protein [...	Rattus norvegicus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other HUGE database (FASTA output)
No significant homologues
Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method interpro_ID From To Entry Definition

HMMPfam IPR009231 3 551 PF05934 Mid-1-related chloride channel

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR009231	3	551	PF05934	Mid-1-related chloride channel

Prediction of transmembrane (TM) segments

Method No. N terminal transmembrane region C terminal type length

SOSUI2 1 1 MLCSLLLCECLLLVAGYAHDDDW 23 PRIMARY 23

2 181 DPYNVLMVLLCLLCIVVLVATEL 203 PRIMARY 23

3 216 VLIISFLFSLGWNWMYLYKLAFA 238 SECONDARY 23

4 329 EIPALLHLPVLIIMALAILSFCY 351 PRIMARY 23

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	1	MLCSLLLCECLLLVAGYAHDDDW	23	PRIMARY	23
2	181	DPYNVLMVLLCLLCIVVLVATEL	203	PRIMARY	23
3	216	VLIISFLFSLGWNWMYLYKLAFA	238	SECONDARY	23
4	329	EIPALLHLPVLIIMALAILSFCY	351	PRIMARY	23

Expression profile	Description

: RT-PCR-ELISA Description
: Experimental conditions

Primer_f
: CTGTTCATCCATTCTCTTTCC

Primer_r
: GACTGAGAGATTTAACATAGC

PCR conditions
: 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RT-PCR-ELISA	Description

RH mapping information	Description

Chromosome No.

: 1

Experimental conditions

Panel name	: GeneBridge 4
Primer_f	: CTGTTCATCCATTCTCTTTCC
Primer_r	: GACTGAGAGATTTAACATAGC
PCR product length	: 197 bp
PCR conditions	: 95 °C15 sec62 °C60 sec30 cycles

How to obtain KIAA clone(s) Back to the HUGE Protein Database homepage

	cloned DNA seq.
Warning for N-terminal truncation:	YES
Warning for coding interruption:	NO

Length of 3'UTR	3039 bp
Genome contig ID	gi89161185r_109173653
PolyA signal sequence (AAGAAA,-21)	+--------+--------+----*----+---- AGCCTGATTTCTGAAAGAAATAATCTTTAAACCTG
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* AAACTGCATAAAATTTTTCCTTTAAAATGGCTTATATAGGTATAGCTGCT

	Chr	f/r	start	end	exon	identity	class
ContigView(URL based/DAS)	1	r	109273653	109294583	11	99.3	Perfect prediction

Gene/Protein Characteristic Table for KIAA0761

Features of the cloned DNA sequence

Features of the protein sequence

Expression profile

RH mapping information